##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551905_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	224827
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.987225733564028	33.0	31.0	34.0	30.0	34.0
2	32.08819225448901	33.0	31.0	34.0	30.0	34.0
3	32.09383214649486	33.0	31.0	34.0	30.0	34.0
4	35.72120341418068	37.0	35.0	37.0	35.0	37.0
5	35.76904909107892	37.0	35.0	37.0	35.0	37.0
6	35.67433181957683	37.0	35.0	37.0	35.0	37.0
7	35.790234269015734	37.0	35.0	37.0	35.0	37.0
8	34.99449354392488	37.0	35.0	37.0	32.0	37.0
9	36.74686314366157	39.0	37.0	39.0	32.0	39.0
10	36.69428049122214	38.0	35.0	39.0	32.0	39.0
11	37.07897627953938	39.0	37.0	39.0	34.0	39.0
12	37.07890956157401	39.0	37.0	39.0	34.0	39.0
13	37.09475285441695	39.0	37.0	39.0	33.0	39.0
14	38.12668407264252	40.0	37.0	41.0	33.0	41.0
15	38.071188069048645	40.0	37.0	41.0	33.0	41.0
16	38.33891392048108	40.0	37.0	41.0	34.0	41.0
17	38.18709051848755	40.0	37.0	41.0	34.0	41.0
18	38.039261298687435	39.0	37.0	41.0	34.0	41.0
19	37.94549587015794	39.0	37.0	41.0	34.0	41.0
20	37.8018254035325	40.0	35.0	41.0	33.0	41.0
21	37.878817935568236	40.0	36.0	41.0	34.0	41.0
22	37.98765717640675	40.0	36.0	41.0	34.0	41.0
23	37.934216086146236	40.0	36.0	41.0	34.0	41.0
24	37.78066246491747	40.0	35.0	41.0	33.0	41.0
25	37.59779741756996	39.0	35.0	41.0	33.0	41.0
26	37.65699404430963	39.0	35.0	41.0	33.0	41.0
27	37.685291357354764	40.0	35.0	41.0	33.0	41.0
28	37.51262971084434	40.0	35.0	41.0	33.0	41.0
29	37.51564536287901	40.0	35.0	41.0	33.0	41.0
30	37.245188522730814	39.0	35.0	41.0	33.0	41.0
31	37.094953008313055	39.0	35.0	41.0	32.0	41.0
32	36.9242395263914	39.0	35.0	41.0	32.0	41.0
33	36.80017969372006	39.0	35.0	41.0	31.0	41.0
34	36.503373705115486	39.0	35.0	41.0	30.0	41.0
35	36.45432710484061	39.0	35.0	41.0	30.0	41.0
36	36.35542439297771	39.0	35.0	41.0	30.0	41.0
37	36.36940403065468	39.0	35.0	41.0	30.0	41.0
38	36.128147420016276	39.0	35.0	41.0	30.0	41.0
39	36.041498574459474	39.0	35.0	41.0	30.0	41.0
40	35.94172408118242	39.0	35.0	41.0	29.0	41.0
41	35.65487686087525	39.0	35.0	40.0	27.0	41.0
42	35.95140263402527	39.0	35.0	41.0	29.0	41.0
43	35.865051795380445	39.0	35.0	41.0	29.0	41.0
44	36.025842091919564	39.0	35.0	41.0	29.0	41.0
45	36.01740004536822	39.0	35.0	41.0	29.0	41.0
46	35.91310652190351	39.0	35.0	41.0	29.0	41.0
47	35.67939348921615	39.0	35.0	41.0	28.0	41.0
48	35.73735360966432	39.0	35.0	41.0	28.0	41.0
49	35.74869121591268	39.0	35.0	41.0	28.0	41.0
50	35.62131772429468	38.0	35.0	41.0	28.0	41.0
51	34.83096336294128	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	3.0
12	7.0
13	4.0
14	6.0
15	6.0
16	17.0
17	29.0
18	49.0
19	119.0
20	279.0
21	505.0
22	904.0
23	1166.0
24	1543.0
25	2078.0
26	2916.0
27	3361.0
28	3218.0
29	3030.0
30	3350.0
31	4141.0
32	5202.0
33	7246.0
34	13256.0
35	17510.0
36	17301.0
37	25571.0
38	47038.0
39	64943.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.78106277270968	14.546295596169498	16.47088650384518	20.20175512727564
2	28.93335764832516	35.51130424726567	18.50356051541852	17.051777588990646
3	25.731784883488196	33.69568601635925	25.265648698777284	15.306880401375281
4	25.105970368327647	18.398590916571408	39.45300164126195	17.042437073838997
5	32.20342752427422	21.419580388476472	25.402642921001483	20.974349166247826
6	21.194073665529494	37.576447668651895	26.300666734867256	14.928811930951355
7	61.976541963376285	5.249369515227264	26.22861133226881	6.545477189127641
8	57.542465984957325	16.231591401388624	17.12516735089647	9.100775262757587
9	54.671814328350244	6.789664942377917	17.282621749167138	21.255898980104703
10	40.63302005542039	17.140290089713424	25.135326273089976	17.091363581776207
11	31.584729592086358	20.035849786724906	27.265408514101953	21.114012107086783
12	25.614361264438877	15.882878835727027	32.776312453575414	25.72644744625868
13	24.59179724855111	20.149270327852083	37.81707713041583	17.44185529318098
14	19.760526982969125	28.27952158770966	28.49123993114706	23.46871149817415
15	15.676497929519142	21.073981327865425	42.04299305688374	21.206527685731697
16	17.20078104498125	22.47861689209926	30.78678272627398	29.53381933664551
17	17.561502844409258	24.099418664128418	33.562250085621386	24.776828405840938
18	17.66291415177003	22.71124019801892	33.3625409759504	26.263304674260652
19	18.975923710230532	24.17280842603424	29.465767011969202	27.385500851766025
20	21.59349188487148	24.199940398617603	35.96454162533859	18.242026091172324
21	20.886726238396633	29.41417178541723	29.9826088503605	19.716493125825636
22	18.174418552931808	21.256343766540496	33.16016314766465	27.40907453286305
23	19.989147210966653	27.15198797297478	31.240909677218482	21.617955138840088
24	22.727697296143255	21.4115742326322	28.827498476606454	27.033229994618086
25	17.165198130117822	32.17807469743403	27.140423525644163	23.516303646803987
26	17.615766789575986	24.24130553714634	31.86361068732848	26.279316985949198
27	23.48249987768373	25.399529415950933	26.620468182202316	24.49750252416302
28	17.997838337922047	24.89291766558287	33.408798765272856	23.70044523122223
29	20.497538107077887	25.297228535718574	28.726531955681477	25.47870140152206
30	20.955668135944528	25.719330863285993	28.55795789651599	24.767043104253492
31	25.917705613649606	23.24275998879138	24.937396309162153	25.90213808839686
32	24.622487512620815	30.72273347951981	23.14624133222433	21.508537675635043
33	25.50805730628439	20.413473470713036	25.651278538609684	28.42719068439289
34	23.151578769453845	25.190924577564083	28.847958652652927	22.80953800032914
35	19.71160047503191	21.70068541589756	25.461799516961932	33.1259145921086
36	23.61682538129317	26.22683218652564	28.472114114407965	21.684228317773222
37	19.21566359912288	24.206167408718706	31.007396798427234	25.570772193731177
38	26.62269211438128	22.777958163387847	23.680874628047345	26.91847509418353
39	23.30013743900866	25.93416271177394	30.366014758013936	20.39968509120346
40	24.37251753570523	20.082552362483153	29.924341827271633	25.62058827453998
41	19.010617052222376	23.0488331027857	31.98281345212096	25.957736392870963
42	19.769867498120778	20.117245704474996	31.92765993408265	28.185226863321578
43	23.757377895003714	21.697127124411214	29.742424175032355	24.803070805552714
44	22.019597290361034	20.79421065975172	30.775218278943367	26.41097377094388
45	21.8412379296081	19.153393498111882	27.200024908040405	31.805343664239615
46	27.425531630987383	23.89081382574157	26.84730926445667	21.836345278814377
47	18.315860639513936	21.08999363955397	37.84687782161395	22.747267899318143
48	21.875486485164146	21.406236795402688	28.708295711813975	28.00998100761919
49	20.117245704474996	18.149955298963203	37.18103252723205	24.551766469329753
50	22.429690384162047	18.455078793917103	31.900083175063493	27.215147646857364
51	20.111908267245482	18.858944877617013	27.467341555951908	33.5618052991856
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30.0
1	106.0
2	182.0
3	2380.0
4	4578.0
5	3006.0
6	1434.0
7	1427.0
8	1420.0
9	1404.5
10	1389.0
11	1274.5
12	1160.0
13	1132.5
14	1105.0
15	982.0
16	859.0
17	847.0
18	835.0
19	826.5
20	818.0
21	816.0
22	814.0
23	757.0
24	700.0
25	764.5
26	955.5
27	1082.0
28	1351.0
29	1620.0
30	1862.5
31	2105.0
32	2379.5
33	2654.0
34	2779.0
35	2904.0
36	3061.5
37	3219.0
38	3772.0
39	4325.0
40	4926.5
41	5528.0
42	6558.0
43	7588.0
44	9038.5
45	10489.0
46	26573.5
47	42658.0
48	32331.5
49	22005.0
50	22261.5
51	22518.0
52	18929.0
53	15340.0
54	13798.5
55	12257.0
56	11111.0
57	9965.0
58	9224.5
59	8484.0
60	7989.5
61	7495.0
62	6934.0
63	6373.0
64	5756.5
65	5140.0
66	4409.5
67	3679.0
68	3370.0
69	3061.0
70	2694.5
71	2328.0
72	2206.5
73	2085.0
74	1776.0
75	1192.0
76	917.0
77	760.0
78	603.0
79	545.0
80	487.0
81	317.5
82	148.0
83	114.0
84	80.0
85	59.0
86	38.0
87	30.5
88	23.0
89	15.0
90	7.0
91	4.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	224827.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.23853006978699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.21876917789724	26.89579098595809
2	10.264624297321845	7.439497925071277
3	3.6109679161450274	3.9256850823077296
4	1.9883643861845497	2.882216103937694
5	1.3955372265998969	2.528610887482375
6	1.0580062351179518	2.3004354459206415
7	0.8640793381937797	2.1919075555871848
8	0.8235756192159462	2.3876135873360402
9	0.6959275351645924	2.269745181850934
>10	4.983184819697081	28.134965996076982
>50	0.05645972948425265	1.405080350669626
>100	0.025775093894984905	1.9570603174885577
>500	0.0024547708471414193	0.6680692265608668
>1k	0.008591697964994966	5.672806202102061
>5k	0.0036821562707121287	9.340515151649935
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC	7949	3.5356073781173967	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	6908	3.0725846984570357	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG	6143	2.7323230750755023	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	2786	1.2391750101188914	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2712	1.2062608138702202	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGAGTCTT	1772	0.7881615642249373	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCT	1579	0.7023177821169166	No Hit
GAACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT	1423	0.6329310981332313	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTA	1263	0.5617652684063746	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTC	1219	0.5421946652314891	No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	926	0.41187223954418284	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	576	0.256196987016684	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATG	458	0.20371218759312718	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT	344	0.1530065339127418	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACGAGTCT	316	0.14055251371054187	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTAT	297	0.13210157143047765	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGT	291	0.12943285281572053	No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	285	0.1267641342009634	No Hit
TGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT	251	0.11164139538400637	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCC	237	0.1054143852829064	No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	228	0.10141130736077072	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2642031428609553	0.0
2	0.0	0.0	0.0	2.197689779252492	0.0
3	0.0	0.0	0.0	2.6309117677147316	0.0
4	0.0	0.0	0.0	3.825163347818545	0.0
5	0.0	0.0	0.0	8.478519039083384	0.0
6	0.0	0.0	0.0	9.535776396962998	0.0
7	0.0	0.0	0.0	11.195274588906138	0.0
8	0.0	0.0	0.0	13.07538685300253	0.0
9	0.0	0.0	0.0	13.584667321985348	0.0
10	0.0	0.0	0.0	17.456978031997938	0.0
11	0.0	0.0	0.0	19.211215734764952	0.0
12	0.0	0.0	0.0	24.296903841620445	0.0
13	0.0	0.0	0.0	25.128654476553084	0.0
14	0.0	0.0	0.0	25.513839529949696	0.0
15	0.0	0.0	0.0	26.50615806820355	0.0
16	0.0	0.0	0.0	27.432203427524275	0.0
17	0.0	0.0	0.0	28.471669327972172	0.0
18	0.0	0.0	0.0	29.42662580561943	0.0
19	0.0	0.0	0.0	30.86773385758828	0.0
20	0.0	0.0	0.0	31.61586464259186	0.0
21	0.0	0.0	0.0	32.33419473639732	0.0
22	0.0	0.0	0.0	33.10589920249792	0.0
23	0.0	0.0	0.0	33.806437838871666	0.0
24	0.0	0.0	0.0	34.403341235705675	0.0
25	0.0	0.0	0.0	34.97933967005742	0.0
26	0.0	0.0	0.0	35.471273468044316	0.0
27	0.0	0.0	0.0	36.025477367042214	0.0
28	0.0	0.0	0.0	36.483607395908855	0.0
29	0.0	0.0	0.0	36.9662006787441	0.0
30	0.0	0.0	0.0	37.486156022185945	0.0
31	0.0	0.0	0.0	38.02256846375213	0.0
32	0.0	0.0	0.0	38.51983969896854	0.0
33	0.0	0.0	0.0	38.98597588367945	0.0
34	0.0	0.0	0.0	39.46056301067043	0.0
35	0.0	0.0	0.0	39.987634937084955	0.0
36	0.0	0.0	0.0	40.532498320931204	0.0
37	0.0	0.0	0.0	41.006195875050594	0.0
38	0.0	0.0	0.0	41.47722471055523	0.0
39	0.0	0.0	0.0	41.94647440031669	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATAAG	20	7.021168E-4	45.000004	12
CCTTAGG	30	2.1576889E-6	45.000004	23
CAGTTAT	20	7.021168E-4	45.000004	36
TATATGG	30	2.1576889E-6	45.000004	2
TAACGAG	20	7.021168E-4	45.000004	1
ACGGGTA	20	7.021168E-4	45.000004	5
ACGGGAC	20	7.021168E-4	45.000004	5
TCAATGG	20	7.021168E-4	45.000004	2
CCATCGA	30	2.1576889E-6	45.000004	41
ATGAGTC	20	7.021168E-4	45.000004	38
CACTAGG	20	7.021168E-4	45.000004	2
GTGTAAG	20	7.021168E-4	45.000004	1
TTGCGCG	20	7.021168E-4	45.000004	1
ACTAATC	20	7.021168E-4	45.000004	10
GCGATGT	20	7.021168E-4	45.000004	9
GTATCCA	20	7.021168E-4	45.000004	24
TTAGTCA	20	7.021168E-4	45.000004	32
TTTGCGT	20	7.021168E-4	45.000004	18
ATTGCGG	40	6.7720975E-9	45.000004	2
ACGAGAG	20	7.021168E-4	45.000004	15
>>END_MODULE