FastQCFastQC Report
Sat 18 Jun 2016
SRR3551904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413741
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC99942.4155208209967105No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC97762.362830853118255No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG93512.260109585465303No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC37380.9034637611452576TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34800.8411059092524067No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT22330.5397096251036276No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTAT21360.5162650063687186No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTA17110.41354373871576666No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC17090.41306034451504686TruSeq Adapter, Index 14 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT17010.4111267677121677No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC16540.3997670039952531No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT8100.19577465129150848Illumina PCR Primer Index 8 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG5910.1428429863126932No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATG5660.13680055880369604No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT5370.1297913428932593No Hit
TGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTT4440.10731351255978983TruSeq Adapter, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAACC302.16212E-645.0000048
TACGAAT453.8380676E-1045.00000412
CTAGATC207.028046E-445.019
TCGCAAA253.8864626E-545.037
CACGGGT207.028046E-445.04
CGACGAA207.028046E-445.041
ATAGCGG406.7975634E-945.02
AACGGGC253.8864626E-545.04
TTAAGCG253.8864626E-545.01
TCCACTA253.8864626E-545.027
TTGCCGT207.028046E-445.027
TATACGG253.8864626E-545.02
ATTTGCG207.028046E-445.01
CGTTTTT16350.042.5229381
CGATGAA6800.042.35294319
CACCGAG750.042.016
GATGAAT7300.041.91780520
TACGGCT12300.041.8902447
TGAATGA7200.041.87500422
CATCGAA603.6379788E-1241.25000442
Produced by FastQC (version 0.11.5)