##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551904_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413741
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01404743547292	33.0	31.0	34.0	30.0	34.0
2	32.11698623051619	33.0	31.0	34.0	30.0	34.0
3	32.1265912732845	33.0	31.0	34.0	30.0	34.0
4	35.720172765087334	37.0	35.0	37.0	35.0	37.0
5	35.76300149127111	37.0	35.0	37.0	35.0	37.0
6	35.66665377615465	37.0	35.0	37.0	35.0	37.0
7	35.80812150596629	37.0	35.0	37.0	35.0	37.0
8	35.103692889996395	37.0	35.0	37.0	32.0	37.0
9	36.82249039858269	39.0	37.0	39.0	32.0	39.0
10	36.708303020488664	38.0	35.0	39.0	32.0	39.0
11	37.05387186669922	39.0	37.0	39.0	34.0	39.0
12	37.058091898071496	39.0	37.0	39.0	33.0	39.0
13	37.17234211741162	39.0	37.0	39.0	34.0	39.0
14	38.263950152390024	40.0	38.0	41.0	34.0	41.0
15	38.2239782859325	40.0	38.0	41.0	33.0	41.0
16	38.41163916556493	40.0	38.0	41.0	34.0	41.0
17	38.26251688858489	40.0	37.0	41.0	34.0	41.0
18	38.161110453157896	40.0	37.0	41.0	34.0	41.0
19	38.110687604080816	40.0	37.0	41.0	34.0	41.0
20	38.04042142306419	40.0	37.0	41.0	34.0	41.0
21	38.07028551678466	40.0	37.0	41.0	34.0	41.0
22	38.153729023712906	40.0	37.0	41.0	34.0	41.0
23	38.14693008427978	40.0	37.0	41.0	34.0	41.0
24	38.01594234073974	40.0	36.0	41.0	34.0	41.0
25	37.81000916032011	40.0	36.0	41.0	33.0	41.0
26	37.901977323978045	40.0	36.0	41.0	34.0	41.0
27	37.89858631366	40.0	36.0	41.0	34.0	41.0
28	37.737323591328874	40.0	36.0	41.0	33.0	41.0
29	37.80701936718865	40.0	36.0	41.0	33.0	41.0
30	37.47855300779957	40.0	36.0	41.0	33.0	41.0
31	37.422455110806034	40.0	36.0	41.0	33.0	41.0
32	37.324628209435375	40.0	36.0	41.0	33.0	41.0
33	37.19962246912924	40.0	36.0	41.0	32.0	41.0
34	37.06861297285016	40.0	36.0	41.0	32.0	41.0
35	36.88686400429254	40.0	35.0	41.0	31.0	41.0
36	36.81556094271537	40.0	35.0	41.0	31.0	41.0
37	36.73917257414663	40.0	35.0	41.0	31.0	41.0
38	36.59566975474995	40.0	35.0	41.0	31.0	41.0
39	36.54261724122096	40.0	35.0	41.0	31.0	41.0
40	36.40859136512939	39.0	35.0	41.0	30.0	41.0
41	36.20371440103833	39.0	35.0	41.0	29.0	41.0
42	36.28766547187733	39.0	35.0	41.0	30.0	41.0
43	36.247954638288206	39.0	35.0	41.0	30.0	41.0
44	36.37522749739571	39.0	35.0	41.0	31.0	41.0
45	36.334866498606615	39.0	35.0	41.0	31.0	41.0
46	36.10217744917714	39.0	35.0	41.0	30.0	41.0
47	35.84480870882992	38.0	35.0	40.0	29.0	41.0
48	35.75979658772034	38.0	35.0	40.0	30.0	41.0
49	35.66547429430489	37.0	35.0	40.0	30.0	41.0
50	35.50101875327802	37.0	35.0	40.0	29.0	41.0
51	34.51015490367162	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	6.0
11	7.0
12	8.0
13	17.0
14	23.0
15	29.0
16	31.0
17	66.0
18	101.0
19	248.0
20	586.0
21	1205.0
22	1662.0
23	2107.0
24	2636.0
25	3394.0
26	4319.0
27	4820.0
28	4935.0
29	5063.0
30	5902.0
31	7121.0
32	9067.0
33	12486.0
34	22776.0
35	29666.0
36	29995.0
37	48557.0
38	93035.0
39	123818.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.01371147650342	15.956359171559017	18.46058282838781	18.569346523549758
2	29.158338187416767	33.562301053074265	19.810702830998135	17.46865792851083
3	27.636129849350198	32.01302263976739	24.798605891125124	15.552241619757288
4	25.78908060839994	20.694105732813522	36.00368346380948	17.51313019497705
5	31.106416816317456	23.80764777964959	24.29345895137296	20.792476452659997
6	22.484356155179206	37.134825893493755	25.15148365765056	15.229334293676477
7	66.22186343630435	6.019466284462985	21.593702340353023	6.164967938879638
8	63.77153823285582	14.119219511723518	14.304359490599191	7.80488276482147
9	59.61579828926792	7.508320422679889	14.760441918978298	18.115439369073886
10	39.40363657457201	20.6141039925944	23.23965959380385	16.742599839029733
11	30.592085386751616	22.678680623868555	26.340391694320843	20.388842295058986
12	25.52152191830155	19.05564108947385	30.94665503297957	24.476181959245036
13	24.576486255894388	20.882146076893516	34.98299660898968	19.558371058222416
14	19.973606676640703	28.3097396680532	27.328449440592063	24.388204214714033
15	17.317355543685544	22.90805117211009	38.08227852690451	21.692314757299858
16	18.577805922062353	24.004630916442895	29.40656110948637	28.011002052008383
17	18.525841045484977	26.752243553334093	31.65941978194088	23.062495619240057
18	18.617685943621733	25.351367159648184	30.91789307803674	25.113053818693338
19	20.37675744004099	25.821468019848165	28.03444667074329	25.767327869367552
20	23.09923357849476	24.769602239081937	33.78611256800752	18.34505161441578
21	22.287856412586617	29.465293504873824	28.833738981633438	19.41311110090612
22	19.583990950860564	22.793245049439143	31.33917112396403	26.28359287573627
23	21.292789450404964	27.056298505586827	30.064460616665983	21.586451427342226
24	22.73185398594773	23.812723418757145	27.904897024950394	25.550525570344735
25	19.278485816005666	31.400078793254714	26.33966660301976	22.981768787719854
26	20.08357885730445	24.038951904694	30.849009404434174	25.02845983356738
27	23.62782513698183	28.026470666431415	26.201174164513546	22.144530032073206
28	19.824721262819008	25.938932810623072	31.322735721139555	22.913610205418365
29	26.63574555096063	21.36674876311509	28.064900505388636	23.93260518053565
30	20.971090609825954	29.6323062012225	27.221619322232993	22.174983866718552
31	28.287986929020814	23.28679052837403	23.260687241535162	25.164535301069996
32	28.837122741038474	26.766745379355683	24.605973302138295	19.790158577467544
33	23.45839546962955	24.76573508547618	23.402321742346057	28.373547702548212
34	30.118359070046235	21.971233211115166	25.507745183581033	22.402662535257566
35	23.397004406138137	24.61080724414549	26.926507162693568	25.065681187022797
36	27.778489441462174	27.77824774436181	23.67326419184949	20.769998622326526
37	24.843561551792064	26.446255024278475	29.029271935824585	19.680911488104876
38	24.23424316178479	24.385545546610075	24.796672314322247	26.58353897728289
39	29.807294901883065	23.317486060119737	25.54182447473178	21.333394563265422
40	22.150814156682564	21.757331277296664	30.955839522793244	25.136015043227527
41	24.073997984246184	26.71598898828011	25.679350124836553	23.530662902637157
42	21.863436304354657	23.20267993744879	32.95660811957239	21.977275638624164
43	24.486091540359787	26.256764497596325	25.687084432048067	23.570059529995817
44	24.0793153204541	22.56677486640193	28.482311397710163	24.87159841543381
45	22.764966488697034	20.657851167759542	27.433104285047893	29.14407805849553
46	27.49860419924542	24.973111197584963	25.946425420734226	21.58185918243539
47	19.941944356493554	22.867929453450348	34.93489888601806	22.255227304038034
48	23.514469196913048	23.92777123852845	26.752243553334093	25.805516011224412
49	21.316959160440952	20.76613146872077	34.38769665080328	23.529212720034998
50	23.102859035000158	21.134961243869956	30.243316470932296	25.518863250197587
51	22.00482910806519	20.770723713627607	27.01690187822817	30.207545300079037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	95.0
1	337.5
2	580.0
3	3190.0
4	5800.0
5	3864.0
6	1928.0
7	1891.5
8	1855.0
9	1807.0
10	1759.0
11	1673.0
12	1587.0
13	1470.5
14	1354.0
15	1292.5
16	1231.0
17	1213.5
18	1196.0
19	1103.0
20	1010.0
21	1114.0
22	1218.0
23	1230.5
24	1243.0
25	1559.5
26	2227.0
27	2578.0
28	3258.0
29	3938.0
30	4532.0
31	5126.0
32	5791.0
33	6456.0
34	7232.0
35	8008.0
36	8581.5
37	9155.0
38	10194.5
39	11234.0
40	12587.0
41	13940.0
42	15213.5
43	16487.0
44	17693.5
45	18900.0
46	20656.5
47	22413.0
48	44857.0
49	67301.0
50	52996.5
51	38692.0
52	38923.5
53	39155.0
54	33491.0
55	27827.0
56	24852.5
57	21878.0
58	19910.5
59	17943.0
60	17305.5
61	16668.0
62	14747.0
63	12826.0
64	11425.5
65	10025.0
66	7993.0
67	5961.0
68	5202.0
69	4443.0
70	3665.0
71	2887.0
72	2802.5
73	2718.0
74	2292.0
75	1517.0
76	1168.0
77	940.0
78	712.0
79	552.0
80	392.0
81	298.5
82	205.0
83	126.0
84	47.0
85	49.5
86	52.0
87	28.0
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	413741.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.552306603618284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.28301937740986	23.855313156123458
2	9.820784723221346	6.393783907968635
3	3.4918584895538416	3.410041445052123
4	1.9043130343392243	2.4795912705230845
5	1.3218587526951553	2.1514775702204565
6	0.9771518029248891	1.908512705225363
7	0.8698607321793079	1.9821181279444104
8	0.7900036345874951	2.0573152424851973
9	0.7185189918716789	2.105050547153665
>10	6.719990008575858	38.08712310514228
>50	0.05727039748827793	1.3108092307304244
>100	0.03421348421377643	2.156273921109191
>500	0.002975085583806646	0.6062553220814523
>1k	0.005950171167613292	4.44571095210049
>5k	0.0022313141878549845	7.050623496139766
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	9994	2.4155208209967105	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	9776	2.362830853118255	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	9351	2.260109585465303	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	3738	0.9034637611452576	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3480	0.8411059092524067	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	2233	0.5397096251036276	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTAT	2136	0.5162650063687186	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTA	1711	0.41354373871576666	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1709	0.41306034451504686	TruSeq Adapter, Index 14 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	1701	0.4111267677121677	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC	1654	0.3997670039952531	No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	810	0.19577465129150848	Illumina PCR Primer Index 8 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	591	0.1428429863126932	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATG	566	0.13680055880369604	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	537	0.1297913428932593	No Hit
TGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTT	444	0.10731351255978983	TruSeq Adapter, Index 14 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4169710035988699E-4	0.0	0.0	0.20230047300122542	0.0
2	2.4169710035988699E-4	0.0	0.0	1.7153243212541178	0.0
3	2.4169710035988699E-4	0.0	0.0	2.108323806439294	0.0
4	2.4169710035988699E-4	0.0	0.0	2.92985225056255	0.0
5	2.4169710035988699E-4	0.0	0.0	6.391196424816491	0.0
6	2.4169710035988699E-4	0.0	0.0	7.249221131094091	0.0
7	2.4169710035988699E-4	0.0	0.0	8.541575526718406	0.0
8	2.4169710035988699E-4	0.0	0.0	9.974114240551456	0.0
9	2.4169710035988699E-4	0.0	0.0	10.37267275904491	0.0
10	2.4169710035988699E-4	0.0	0.0	13.51570185212488	0.0
11	2.4169710035988699E-4	0.0	0.0	14.789928965222204	0.0
12	2.4169710035988699E-4	0.0	0.0	18.40716776920827	0.0
13	2.4169710035988699E-4	0.0	0.0	19.028812711333902	0.0
14	2.4169710035988699E-4	0.0	0.0	19.322474688271164	0.0
15	2.4169710035988699E-4	0.0	0.0	20.087687708010566	0.0
16	2.4169710035988699E-4	0.0	0.0	20.842991146635214	0.0
17	2.4169710035988699E-4	0.0	0.0	21.648809279235078	0.0
18	2.4169710035988699E-4	0.0	0.0	22.38791901213561	0.0
19	2.4169710035988699E-4	0.0	0.0	23.46008734933207	0.0
20	2.4169710035988699E-4	0.0	0.0	24.033634568486082	0.0
21	2.4169710035988699E-4	0.0	0.0	24.646820112099114	0.0
22	2.4169710035988699E-4	0.0	0.0	25.33976569883091	0.0
23	2.4169710035988699E-4	0.0	0.0	25.947392209135668	0.0
24	2.4169710035988699E-4	0.0	0.0	26.448671995282073	0.0
25	2.4169710035988699E-4	0.0	0.0	26.913213822173777	0.0
26	2.4169710035988699E-4	0.0	0.0	27.340534295610055	0.0
27	2.4169710035988699E-4	0.0	0.0	27.79855030079204	0.0
28	2.4169710035988699E-4	0.0	0.0	28.234088475640558	0.0
29	2.4169710035988699E-4	0.0	0.0	28.652224459263163	0.0
30	2.4169710035988699E-4	0.0	0.0	29.10927367604371	0.0
31	2.4169710035988699E-4	0.0	0.0	29.567773075426413	0.0
32	2.4169710035988699E-4	0.0	0.0	29.985909059049018	0.0
33	2.4169710035988699E-4	0.0	0.0	30.422414022298973	0.0
34	2.4169710035988699E-4	0.0	0.0	30.846350736330216	0.0
35	2.4169710035988699E-4	0.0	0.0	31.30581692411436	0.0
36	2.4169710035988699E-4	0.0	0.0	31.739663219260358	0.0
37	2.4169710035988699E-4	0.0	0.0	32.17834345641355	0.0
38	2.4169710035988699E-4	0.0	0.0	32.616781996466386	0.0
39	2.4169710035988699E-4	0.0	0.0	33.03830173949403	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAACC	30	2.16212E-6	45.000004	8
TACGAAT	45	3.8380676E-10	45.000004	12
CTAGATC	20	7.028046E-4	45.0	19
TCGCAAA	25	3.8864626E-5	45.0	37
CACGGGT	20	7.028046E-4	45.0	4
CGACGAA	20	7.028046E-4	45.0	41
ATAGCGG	40	6.7975634E-9	45.0	2
AACGGGC	25	3.8864626E-5	45.0	4
TTAAGCG	25	3.8864626E-5	45.0	1
TCCACTA	25	3.8864626E-5	45.0	27
TTGCCGT	20	7.028046E-4	45.0	27
TATACGG	25	3.8864626E-5	45.0	2
ATTTGCG	20	7.028046E-4	45.0	1
CGTTTTT	1635	0.0	42.522938	1
CGATGAA	680	0.0	42.352943	19
CACCGAG	75	0.0	42.0	16
GATGAAT	730	0.0	41.917805	20
TACGGCT	1230	0.0	41.890244	7
TGAATGA	720	0.0	41.875004	22
CATCGAA	60	3.6379788E-12	41.250004	42
>>END_MODULE