##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551903_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458888
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04114293683862	33.0	31.0	34.0	30.0	34.0
2	32.16283711929708	33.0	31.0	34.0	30.0	34.0
3	32.16220515681386	33.0	31.0	34.0	30.0	34.0
4	35.761425445860425	37.0	35.0	37.0	35.0	37.0
5	35.797588954167466	37.0	35.0	37.0	35.0	37.0
6	35.69599989539931	37.0	35.0	37.0	35.0	37.0
7	35.840377172643436	37.0	35.0	37.0	35.0	37.0
8	35.28462718571852	37.0	35.0	37.0	32.0	37.0
9	36.99218545701783	39.0	37.0	39.0	32.0	39.0
10	36.82358440403759	39.0	35.0	39.0	33.0	39.0
11	37.125176513659106	39.0	37.0	39.0	34.0	39.0
12	37.10317768170011	39.0	37.0	39.0	34.0	39.0
13	37.21573891668555	39.0	37.0	39.0	34.0	39.0
14	38.29912527675599	40.0	38.0	41.0	34.0	41.0
15	38.254545771517236	40.0	38.0	41.0	33.0	41.0
16	38.41477005282335	40.0	38.0	41.0	34.0	41.0
17	38.32047035442199	40.0	38.0	41.0	34.0	41.0
18	38.20898127647705	40.0	37.0	41.0	34.0	41.0
19	38.105943062359444	40.0	37.0	41.0	34.0	41.0
20	38.06225484214013	40.0	36.0	41.0	34.0	41.0
21	38.05889454507418	40.0	36.0	41.0	34.0	41.0
22	38.169102264604874	40.0	37.0	41.0	34.0	41.0
23	38.17786693049285	40.0	36.0	41.0	34.0	41.0
24	38.04407393525217	40.0	36.0	41.0	34.0	41.0
25	37.80199307892122	40.0	36.0	41.0	33.0	41.0
26	37.89966179111243	40.0	36.0	41.0	34.0	41.0
27	37.91091290249473	40.0	36.0	41.0	34.0	41.0
28	37.750150363487386	40.0	36.0	41.0	33.0	41.0
29	37.851573804501314	40.0	36.0	41.0	34.0	41.0
30	37.51327992887153	40.0	36.0	41.0	33.0	41.0
31	37.348472829971584	39.0	35.0	41.0	33.0	41.0
32	37.35060188978574	40.0	35.0	41.0	33.0	41.0
33	37.17857298512927	40.0	35.0	41.0	33.0	41.0
34	37.001475305521176	40.0	35.0	41.0	32.0	41.0
35	36.94809190913687	40.0	35.0	41.0	32.0	41.0
36	36.76369179407612	40.0	35.0	41.0	31.0	41.0
37	36.72261205348582	40.0	35.0	41.0	31.0	41.0
38	36.51701286588449	39.0	35.0	41.0	31.0	41.0
39	36.44687592615192	39.0	35.0	41.0	31.0	41.0
40	36.325327748818886	39.0	35.0	41.0	30.0	41.0
41	36.12366416206133	39.0	35.0	41.0	29.0	41.0
42	36.35476848381305	39.0	35.0	41.0	31.0	41.0
43	36.22437936925786	39.0	35.0	41.0	30.0	41.0
44	36.304154826450024	39.0	35.0	41.0	31.0	41.0
45	36.33131613814264	39.0	35.0	41.0	31.0	41.0
46	36.20388635135371	39.0	35.0	41.0	30.0	41.0
47	36.037521138055475	39.0	35.0	41.0	30.0	41.0
48	36.05779841704294	39.0	35.0	41.0	30.0	41.0
49	36.02144749917191	39.0	35.0	41.0	30.0	41.0
50	35.85973919562072	38.0	35.0	41.0	30.0	41.0
51	34.95603720297763	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	4.0
10	6.0
11	10.0
12	11.0
13	6.0
14	11.0
15	26.0
16	31.0
17	77.0
18	107.0
19	227.0
20	606.0
21	1094.0
22	1405.0
23	1827.0
24	2512.0
25	3378.0
26	4826.0
27	5805.0
28	5771.0
29	5783.0
30	6300.0
31	7782.0
32	10074.0
33	13865.0
34	25765.0
35	34685.0
36	33917.0
37	52517.0
38	97507.0
39	142897.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.38059831592894	16.163639057896482	17.82635414305887	19.629408483115707
2	30.242891511654257	30.785071738637747	20.934955806209793	18.037080943498196
3	28.224752009204863	30.033908055996232	26.09547427694775	15.645865657851154
4	26.670124300482907	20.689580028242187	34.50384407524276	18.136451596032146
5	29.88005787904674	24.561766705601368	25.341913495231953	20.216261920119944
6	23.57023936123847	35.68801101793902	25.873415735429994	14.868333885392515
7	67.4816077125573	5.846524642178483	20.39735185927721	6.274515785986995
8	65.8132267568557	12.087481041125503	14.351432157737836	7.747860044280958
9	60.764500270218434	6.871175537386029	15.992791269329334	16.371532923066194
10	40.30024755495894	20.277932741758338	22.931085580795312	16.490734122487403
11	30.566717804780254	21.92866233154931	27.335428252645528	20.16919161102491
12	26.10876728090514	18.331488293439794	31.01759034884329	24.54215407681177
13	24.748086679102528	21.196457523404405	34.91570927982427	19.139746517668797
14	20.8070378828821	26.455474974285664	28.884608008925927	23.852879133906313
15	17.68492529767612	22.647574135736825	37.635545056745876	22.03195550984118
16	20.02645525705619	23.12111016195673	30.30478025139032	26.547654329596764
17	19.96739945258974	24.929612454455118	31.367784731786408	23.73520336116874
18	19.538318718292917	23.45997280382141	31.758511880894684	25.243196596990984
19	20.81640836108157	25.29048482418368	28.669304928435697	25.223801886299054
20	23.08406408535416	24.176705427032307	33.80715991701679	18.932070570596746
21	22.96682414881191	28.52308188490438	29.321533794738585	19.188560171545127
22	19.515219399940726	22.428348529488677	31.515751120099022	26.540680950471575
23	21.265537560363313	26.4811893098098	30.386717456111295	21.86655567371559
24	22.97074667456983	22.45079409354788	29.102961942783423	25.475497289098865
25	19.428923833266502	29.55339865065114	27.037970049336657	23.979707466745698
26	20.235656630811878	23.914549955544707	31.04679137393002	24.803002039713395
27	23.396558637401718	26.79368386185736	27.049301790415093	22.76045571032583
28	18.611295130838027	26.29486933630864	31.822579801607365	23.27125573124597
29	24.331427276372448	22.126314046128904	29.421776119663186	24.120482557835462
30	20.440281724516655	29.48758738515716	27.904412405641466	22.167718484684716
31	22.536217987831453	27.66927877826398	24.909128153274875	24.885375080629696
32	23.723871620090307	27.07588779832988	26.797388469517617	22.402852112062202
33	21.751930754345285	24.95576262617458	24.48985373337285	28.80245288610729
34	23.910191593591463	26.43629818169139	25.911333484423217	23.742176740293928
35	21.56452119035582	24.59706943742264	25.608209410575128	28.230199961646413
36	25.858597304788965	28.204485626122278	22.970092920276844	22.96682414881191
37	20.263550147312635	28.9521626192012	29.892043374418158	20.892243859068007
38	20.40127438503513	27.42521050888234	24.673994525897385	27.499520580185145
39	23.355154198845906	25.361308205923887	28.863469953452693	22.420067641777514
40	21.072897962029952	22.587646658879727	28.117972141350396	28.221483237739932
41	21.608976482278898	27.825743972385418	24.60491448893848	25.960365056397205
42	21.318709576192884	22.97880964418333	29.216715189763082	26.485765589860705
43	26.667073447115637	22.086217116159062	26.35283555028678	24.893873886438524
44	21.93890448213943	23.415299593800665	29.057417060372032	25.588378863687872
45	21.309774934188734	22.288445110789564	27.04799428182912	29.35378567319259
46	25.55416572235491	24.705156813863077	26.495136068060184	23.245541395721833
47	18.68822021931277	23.332926552884363	33.868176984362194	24.110676243440665
48	21.215634315998674	24.111547915831313	27.867584247136556	26.805233521033458
49	20.765633444326284	20.814882934397936	33.70212339394362	24.71736022733216
50	22.493288122592006	20.491274559369607	30.443376161503462	26.572061156534925
51	20.700258015027632	21.22783772946776	27.256106065096493	30.815798190408117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	128.0
1	340.0
2	552.0
3	4457.0
4	8362.0
5	5446.0
6	2530.0
7	2447.5
8	2365.0
9	2293.5
10	2222.0
11	2136.0
12	2050.0
13	1940.5
14	1831.0
15	1635.0
16	1439.0
17	1322.5
18	1206.0
19	1202.0
20	1198.0
21	1175.5
22	1153.0
23	1343.0
24	1533.0
25	1783.0
26	2394.0
27	2755.0
28	3274.0
29	3793.0
30	4357.5
31	4922.0
32	5253.0
33	5584.0
34	6796.5
35	8009.0
36	8755.0
37	9501.0
38	9942.5
39	10384.0
40	12126.0
41	13868.0
42	15971.5
43	18075.0
44	21048.5
45	24022.0
46	46116.5
47	68211.0
48	56675.0
49	45139.0
50	45654.0
51	46169.0
52	38871.5
53	31574.0
54	28679.5
55	25785.0
56	23094.0
57	20403.0
58	19472.0
59	18541.0
60	17557.0
61	16573.0
62	15585.5
63	14598.0
64	13081.0
65	11564.0
66	9608.0
67	7652.0
68	7092.5
69	6533.0
70	5919.0
71	5305.0
72	4688.0
73	4071.0
74	3344.0
75	2251.0
76	1885.0
77	1651.0
78	1417.0
79	1090.5
80	764.0
81	536.0
82	308.0
83	223.5
84	139.0
85	121.0
86	103.0
87	58.5
88	14.0
89	9.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	458888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.12330905094426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.77899123867219	24.106810192145037
2	10.419526778645174	6.902584113073076
3	3.842057029731464	3.817849271614446
4	1.9588704841115117	2.595370897439936
5	1.2455433533287106	2.0628258714328176
6	1.0016729988046922	1.9907234584436364
7	0.8121545176716433	1.8830871560170888
8	0.6988927520842512	1.8519712496601295
9	0.6454470913153255	1.9241409133503515
>10	6.477747396512154	37.69465655785544
>50	0.07079616903413855	1.6320785251932992
>100	0.03969878637428329	2.7642549265829306
>500	6.616464395713883E-4	0.12316746759213565
>1k	0.005954817956142494	4.694609116176402
>5k	0.0019849393187141647	5.955870283423271
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC	9582	2.0880912117989574	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC	8995	1.9601732884712608	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	8599	1.8738777217970397	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4953	1.079348337720751	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	4006	0.8729798992346716	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAATCACGT	2364	0.5151583828733809	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT	2041	0.4447708373284984	No Hit
GAACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT	1949	0.4247223723435784	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTC	1633	0.3558602534823312	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	1604	0.34954062865012814	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTA	1603	0.34932271055246594	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	1268	0.2763201478356374	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATG	562	0.12246997088614214	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT	466	0.10154983351057338	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1791809766217465E-4	0.0	0.0	0.2843831174491379	0.0
2	2.1791809766217465E-4	0.0	0.0	1.7322309583166262	0.0
3	2.1791809766217465E-4	0.0	0.0	2.1377765380659333	0.0
4	2.1791809766217465E-4	0.0	0.0	3.0467129234148635	0.0
5	2.1791809766217465E-4	0.0	0.0	6.294564250971915	0.0
6	2.1791809766217465E-4	0.0	0.0	7.271055246596119	0.0
7	2.1791809766217465E-4	0.0	0.0	8.572026289639302	0.0
8	2.1791809766217465E-4	0.0	0.0	10.24541936158714	0.0
9	2.1791809766217465E-4	0.0	0.0	10.763628597827793	0.0
10	4.358361953243493E-4	0.0	0.0	13.688743222747163	0.0
11	4.358361953243493E-4	0.0	0.0	15.252741409668591	0.0
12	4.358361953243493E-4	0.0	0.0	18.69388608985199	0.0
13	4.358361953243493E-4	0.0	0.0	19.392967347152247	0.0
14	4.358361953243493E-4	0.0	0.0	19.70110353724656	0.0
15	4.358361953243493E-4	0.0	0.0	20.468828995310403	0.0
16	4.358361953243493E-4	0.0	0.0	21.340937222154427	0.0
17	4.358361953243493E-4	0.0	0.0	22.260115758093477	0.0
18	4.358361953243493E-4	0.0	0.0	23.140504872648663	0.0
19	4.358361953243493E-4	0.0	0.0	24.29743205313715	0.0
20	4.358361953243493E-4	0.0	0.0	24.967748121546	0.0
21	4.358361953243493E-4	0.0	0.0	25.717822213699204	0.0
22	4.358361953243493E-4	0.0	0.0	26.449809103746446	0.0
23	4.358361953243493E-4	0.0	0.0	27.15150537821865	0.0
24	4.358361953243493E-4	0.0	0.0	27.756446017328848	0.0
25	4.358361953243493E-4	0.0	0.0	28.355720785899827	0.0
26	4.358361953243493E-4	0.0	0.0	28.834486846463626	0.0
27	4.358361953243493E-4	0.0	0.0	29.327199665277803	0.0
28	4.358361953243493E-4	0.0	0.0	29.833205488049373	0.0
29	4.358361953243493E-4	0.0	0.0	30.34095465560224	0.0
30	4.358361953243493E-4	0.0	0.0	30.87420024058158	0.0
31	4.358361953243493E-4	0.0	0.0	31.398729101654435	0.0
32	4.358361953243493E-4	0.0	0.0	31.934807621903385	0.0
33	4.358361953243493E-4	0.0	0.0	32.435801328428724	0.0
34	4.358361953243493E-4	0.0	0.0	32.905196910793045	0.0
35	4.358361953243493E-4	0.0	0.0	33.414035668834224	0.0
36	4.358361953243493E-4	0.0	0.0	33.90631265145308	0.0
37	4.358361953243493E-4	0.0	0.0	34.42168895242412	0.0
38	4.358361953243493E-4	0.0	0.0	34.89413538815572	0.0
39	4.358361953243493E-4	0.0	0.0	35.38597653457924	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	30	2.1626402E-6	45.000004	1
ACGCAAA	20	7.0288527E-4	45.0	28
GCGAAAA	25	3.88713E-5	45.0	33
CGATTAC	25	3.88713E-5	45.0	10
AGACCTT	20	7.0288527E-4	45.0	18
CATACGG	25	3.88713E-5	45.0	2
TCACTAG	20	7.0288527E-4	45.0	38
CAGTAAT	20	7.0288527E-4	45.0	36
CGATAGT	20	7.0288527E-4	45.0	10
AATACGG	35	1.2097917E-7	45.0	2
CGTTTTT	2365	0.0	43.85835	1
TACGGCT	1130	0.0	42.809734	7
CTACGAA	80	0.0	42.1875	11
TACGAAT	80	0.0	42.1875	12
CGAATAT	75	0.0	42.000004	14
AGGGATA	145	0.0	41.89655	6
CGAACAC	70	0.0	41.785713	34
ACGGCTG	1160	0.0	41.702583	8
GCGAACA	65	0.0	41.53846	33
TAAGGGT	60	3.6379788E-12	41.250004	4
>>END_MODULE