##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551901_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	321102
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.014503179674996	33.0	31.0	34.0	30.0	34.0
2	32.11540881090744	33.0	31.0	34.0	30.0	34.0
3	32.12131347671456	33.0	31.0	34.0	30.0	34.0
4	35.7453270300403	37.0	35.0	37.0	35.0	37.0
5	35.76513382040598	37.0	35.0	37.0	35.0	37.0
6	35.66404444693586	37.0	35.0	37.0	35.0	37.0
7	35.806939850888504	37.0	35.0	37.0	35.0	37.0
8	35.09432516770372	37.0	35.0	37.0	32.0	37.0
9	36.860860411956324	39.0	37.0	39.0	32.0	39.0
10	36.71979931610516	38.0	35.0	39.0	32.0	39.0
11	37.079663159992776	39.0	37.0	39.0	34.0	39.0
12	37.0912762922685	39.0	37.0	39.0	34.0	39.0
13	37.14792184414921	39.0	37.0	39.0	33.0	39.0
14	38.22838537287217	40.0	38.0	41.0	33.0	41.0
15	38.18829842230818	40.0	37.0	41.0	33.0	41.0
16	38.39901028333676	40.0	38.0	41.0	34.0	41.0
17	38.26034406512572	40.0	37.0	41.0	34.0	41.0
18	38.14455531264209	40.0	37.0	41.0	34.0	41.0
19	38.06463055353127	40.0	37.0	41.0	34.0	41.0
20	37.9695953310786	40.0	36.0	41.0	33.0	41.0
21	38.0144190942442	40.0	36.0	41.0	34.0	41.0
22	38.0929673437101	40.0	37.0	41.0	34.0	41.0
23	38.06786939975459	40.0	36.0	41.0	34.0	41.0
24	37.906372429944376	40.0	36.0	41.0	33.0	41.0
25	37.738447596090964	39.0	36.0	41.0	33.0	41.0
26	37.73446443809132	40.0	36.0	41.0	33.0	41.0
27	37.736326774669735	40.0	36.0	41.0	33.0	41.0
28	37.55582649749924	40.0	36.0	41.0	33.0	41.0
29	37.52868247472766	40.0	36.0	41.0	33.0	41.0
30	37.26411856668597	39.0	35.0	41.0	32.0	41.0
31	37.22703377742898	39.0	35.0	41.0	32.0	41.0
32	37.02952644331085	39.0	35.0	41.0	32.0	41.0
33	36.92559373656969	40.0	35.0	41.0	31.0	41.0
34	36.65622138759647	40.0	35.0	41.0	30.0	41.0
35	36.57733056785694	40.0	35.0	41.0	30.0	41.0
36	36.47472454235726	39.0	35.0	41.0	30.0	41.0
37	36.60365242197184	39.0	35.0	41.0	30.0	41.0
38	36.55776669095801	39.0	35.0	41.0	30.0	41.0
39	36.3572821097346	39.0	35.0	41.0	30.0	41.0
40	36.32182297213969	39.0	35.0	41.0	30.0	41.0
41	36.0758419443043	39.0	35.0	41.0	29.0	41.0
42	36.1742000984111	39.0	35.0	41.0	30.0	41.0
43	36.10572652926484	39.0	35.0	41.0	30.0	41.0
44	36.28892065449608	39.0	35.0	41.0	30.0	41.0
45	36.299683589638185	39.0	35.0	41.0	30.0	41.0
46	36.18922647632217	39.0	35.0	41.0	30.0	41.0
47	36.05880374460452	39.0	35.0	41.0	30.0	41.0
48	36.03019912675723	39.0	35.0	41.0	30.0	41.0
49	36.04376179531737	39.0	35.0	41.0	29.0	41.0
50	35.92741870184552	39.0	35.0	41.0	29.0	41.0
51	35.10321953771699	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	6.0
11	5.0
12	6.0
13	7.0
14	10.0
15	11.0
16	28.0
17	45.0
18	81.0
19	169.0
20	378.0
21	685.0
22	1273.0
23	1846.0
24	2339.0
25	3104.0
26	3778.0
27	4050.0
28	4133.0
29	4236.0
30	4611.0
31	5542.0
32	7071.0
33	9839.0
34	17675.0
35	22243.0
36	23006.0
37	36024.0
38	68885.0
39	99974.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.28839434198479	15.702487060186481	17.512815242508612	18.496303355320116
2	29.294118379829463	34.12996493326108	19.79464469234075	16.7812719945687
3	27.272953765470163	33.101319829836	24.59343137071709	15.032295033976743
4	26.146208992781112	20.501273738562826	36.590242352897214	16.762274915758855
5	31.64508473942859	23.74416851966042	24.291035247366878	20.31971149354411
6	22.305684797976966	38.335170755710024	24.649176897060748	14.709967549252262
7	65.64674153384283	5.653343797298055	22.867188619192653	5.832726049666461
8	62.7436141786722	14.762287372859713	14.686610485141793	7.807487963326295
9	58.69567925456708	7.286158292380613	15.269914232860588	18.748248220191716
10	40.00815940106259	21.219737030600868	22.952208332554765	15.819895235781775
11	32.96522600295233	22.172393818786553	25.223449246656827	19.638930931604285
12	28.253950458109884	17.460183991379687	29.686517056885354	24.59934849362508
13	25.82793006583578	20.293240154218907	34.7210543690167	19.157775410928615
14	19.491625713947595	29.113801844896635	27.307522220353658	24.087050220802112
15	16.342470616813348	22.843520127560712	39.747494565589754	21.066514690036186
16	17.09861663894962	22.45610429084839	32.1028831959938	28.342395874208197
17	17.42841838418945	24.71675666922037	33.32554764529651	24.52927730129367
18	18.134736002890048	24.334946527894562	30.869007355918054	26.661310113297333
19	20.84882685252661	25.42525428057128	27.924771567912998	25.801147298989108
20	25.954369639553786	23.702125804261573	32.93439467832651	17.409109877858125
21	22.90269135664057	29.416820823289797	27.696183767151872	19.984304052917764
22	18.639871442719137	23.37076692141438	31.05088102845825	26.938480607408238
23	22.341810390467824	27.998268462980608	28.882099768920778	20.777821377630783
24	23.95064496639697	22.783103188394964	27.5532385347958	25.71301331041227
25	19.291066390119028	30.906067230973335	25.997035209995577	23.805831168912057
26	19.560139768671636	25.59217943208077	30.287260745806627	24.56042005344096
27	24.266432473170518	27.586249852071926	25.22189210904946	22.925425565708093
28	18.028227790546307	27.26454522239039	30.444220216628988	24.263006770434316
29	21.929168924516198	24.81329920087698	28.940648142957688	24.316883731649135
30	19.66041943058592	26.484107853579236	27.092325803015864	26.763146912818982
31	22.72735766205131	23.395992550653684	23.41779247715679	30.45885731013821
32	24.24494397418889	27.092637230537335	27.265168077433337	21.39725071784044
33	21.837297805681683	22.03972569463909	24.961538701098092	31.161437798581137
34	25.874644194056717	24.198541273489422	26.383516764143483	23.54329776831038
35	20.722698706330075	21.40192213066253	28.656937670895854	29.21844149211154
36	19.551419798070395	27.40219618688143	26.747886964266804	26.29849705078137
37	20.40410835186327	22.3122247759279	31.967723651674547	25.315943220534287
38	19.364251857665167	21.921383236479375	26.303479891124937	32.410885014730525
39	25.230923507172175	20.8086527022566	30.88956157233527	23.07086221823595
40	21.064957552428822	20.117595032108177	32.314965338116856	26.50248207734614
41	21.74324669419686	24.21971834494958	29.373532397805057	24.6635025630485
42	20.316597218329377	21.9624916693138	35.080753156317925	22.64015795603889
43	23.196678936911013	23.379798319537095	29.30564119812396	24.117881545427934
44	22.342744673032243	21.49036754676084	29.459486393731588	26.707401386475325
45	20.559822112599736	19.671942248880416	27.498115863495087	32.27011977502476
46	25.42899141082896	24.416229110998998	27.014468922647634	23.14031055552441
47	18.571668815516563	22.737634770259916	36.09164689101905	22.599049523204464
48	22.396933061768536	22.386033098516982	27.173608386120296	28.043425453594185
49	19.88807294878263	19.48290574334635	35.910707501043284	24.718313806827737
50	20.9743321436802	20.50376515873461	31.056486723844763	27.46541597374043
51	19.220372342744675	20.861283953385527	27.404376179531738	32.513967524338064
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	185.0
2	302.0
3	2534.0
4	4766.0
5	3219.5
6	1673.0
7	1676.0
8	1679.0
9	1607.0
10	1535.0
11	1459.5
12	1384.0
13	1309.0
14	1234.0
15	1187.0
16	1140.0
17	1039.5
18	939.0
19	910.0
20	881.0
21	873.5
22	866.0
23	975.5
24	1085.0
25	1142.0
26	1459.5
27	1720.0
28	2092.0
29	2464.0
30	2956.5
31	3449.0
32	3833.0
33	4217.0
34	4735.5
35	5254.0
36	5731.5
37	6209.0
38	6828.5
39	7448.0
40	8748.5
41	10049.0
42	10807.0
43	11565.0
44	13661.0
45	15757.0
46	34551.0
47	53345.0
48	41525.5
49	29706.0
50	30494.0
51	31282.0
52	26545.5
53	21809.0
54	19857.5
55	17906.0
56	16327.5
57	14749.0
58	14379.5
59	14010.0
60	14367.0
61	14724.0
62	13290.5
63	11857.0
64	10085.0
65	8313.0
66	6766.5
67	5220.0
68	4427.0
69	3634.0
70	3132.5
71	2631.0
72	2232.5
73	1834.0
74	1588.5
75	1147.0
76	951.0
77	733.5
78	516.0
79	350.5
80	185.0
81	148.0
82	111.0
83	76.5
84	42.0
85	29.0
86	16.0
87	18.0
88	20.0
89	10.5
90	1.0
91	7.5
92	14.0
93	7.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	321102.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.17336225344294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.84623941019406	22.793617248383192
2	10.714480134931481	6.894417014769375
3	4.052141660767896	3.9111306466246027
4	2.1891431640990557	2.817283841728288
5	1.4998121178938315	2.4126999290550857
6	1.1612854592082755	2.2417474655258207
7	1.014500121278444	2.284791593563722
8	0.9665707574433049	2.487826489824654
9	0.7998173100220335	2.3159530846721847
>10	6.644175234432079	35.568785795314426
>50	0.07389073749988527	1.6753634755016609
>100	0.02596161047293266	1.741542742223299
>500	9.985234797281793E-4	0.20592674742024247
>1k	0.007988187837825435	4.755590705244695
>5k	0.002995570439184538	7.8933232201487655
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGC	9107	2.836170438053952	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	7993	2.4892401791331102	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCG	7470	2.3263635854027696	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	3193	0.9943880760630578	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2695	0.83929717036954	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCTCGTTT	1957	0.6094636595225194	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	1752	0.5456210176205691	No Hit
GAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	1428	0.44471850066334057	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTA	1372	0.4272785594608567	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTC	1280	0.39862722748534735	No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1126	0.3506673891785165	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	641	0.1996250412641466	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATG	482	0.1501080653499511	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	370	0.11522818294498322	No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	338	0.10526250225784953	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.206476446736551	0.0
2	0.0	0.0	0.0	1.794445378727009	0.0
3	0.0	0.0	0.0	2.1401299275619583	0.0
4	0.0	0.0	0.0	3.011815560164683	0.0
5	0.0	0.0	0.0	6.718425920735467	0.0
6	0.0	0.0	0.0	7.620942877964011	0.0
7	0.0	0.0	0.0	8.968489763377368	0.0
8	0.0	0.0	0.0	10.47922467004254	0.0
9	0.0	0.0	0.0	10.926746018399138	0.0
10	0.0	0.0	0.0	14.21822349284651	0.0
11	0.0	0.0	0.0	15.612484506480806	0.0
12	0.0	0.0	0.0	19.707756413849804	0.0
13	0.0	0.0	0.0	20.35396852090613	0.0
14	0.0	0.0	0.0	20.67941028084534	0.0
15	0.0	0.0	0.0	21.483516141288437	0.0
16	0.0	0.0	0.0	22.261150662406337	0.0
17	0.0	0.0	0.0	23.116330636370996	0.0
18	0.0	0.0	0.0	23.92853361237239	0.0
19	0.0	0.0	0.0	25.097943955503236	0.0
20	0.0	0.0	0.0	25.706161904939865	0.0
21	0.0	0.0	0.0	26.32278839745626	0.0
22	0.0	0.0	0.0	27.090145810365552	0.0
23	0.0	0.0	0.0	27.74819216323785	0.0
24	0.0	0.0	0.0	28.256130450760196	0.0
25	0.0	0.0	0.0	28.703963226638265	0.0
26	0.0	0.0	0.0	29.136224626442687	0.0
27	0.0	0.0	0.0	29.629525820455804	0.0
28	3.114275214729276E-4	0.0	0.0	30.029398758027046	0.0
29	3.114275214729276E-4	0.0	0.0	30.446711636800767	0.0
30	3.114275214729276E-4	0.0	0.0	30.907935796102173	0.0
31	3.114275214729276E-4	0.0	0.0	31.39500843968583	0.0
32	3.114275214729276E-4	0.0	0.0	31.86806684480321	0.0
33	3.114275214729276E-4	0.0	0.0	32.26544836220266	0.0
34	3.114275214729276E-4	0.0	0.0	32.68805550884143	0.0
35	3.114275214729276E-4	0.0	0.0	33.10225411240042	0.0
36	3.114275214729276E-4	0.0	0.0	33.585589625726406	0.0
37	3.114275214729276E-4	0.0	0.0	34.03186526399711	0.0
38	3.114275214729276E-4	0.0	0.0	34.473158061924245	0.0
39	3.114275214729276E-4	0.0	0.0	34.879882404967894	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAACGG	25	3.8845057E-5	45.000004	2
GTAGGGT	50	2.1827873E-11	45.000004	4
CCGTATG	25	3.8845057E-5	45.000004	42
GCTACGA	25	3.8845057E-5	45.000004	10
TCGAATA	30	2.1605974E-6	45.000004	44
TAGTAGG	25	3.8845057E-5	45.000004	2
GTGCAAG	20	7.025687E-4	45.0	1
GTACCGC	20	7.025687E-4	45.0	39
CCGATCG	70	0.0	45.0	40
CGTGACT	20	7.025687E-4	45.0	21
TACGGGT	20	7.025687E-4	45.0	4
TCGATGG	20	7.025687E-4	45.0	2
TGTGCCG	20	7.025687E-4	45.0	1
GTTGTAG	20	7.025687E-4	45.0	1
CGAGGGT	20	7.025687E-4	45.0	4
GAAACGC	20	7.025687E-4	45.0	16
CACGTAG	20	7.025687E-4	45.0	1
CGATCGA	70	0.0	45.0	41
CCCATCG	35	1.2082819E-7	45.0	40
TCTATAC	20	7.025687E-4	45.0	20
>>END_MODULE