FastQCFastQC Report
Sat 18 Jun 2016
SRR3551899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754749
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCG172712.2883104184304983No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGC131731.7453484535918564No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTC131391.7408436447083735No Hit
GCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC49380.6542572431364599No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTTCGTT45810.6069567498598872No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT36010.47711225851243255No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34610.45856304546279625No Hit
GAACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT29470.39046093469484555No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTC20030.2653862409887261No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTA18100.23981482585601305No Hit
CCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC11540.15289851328057408No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTGTTCGT8130.10771793006681692No Hit
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT7780.10308062680440783Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATCGAATGC7600.1006957279837403No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7550.10003325608911043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC650.045.00000412
CCATACT302.1645064E-645.00000411
CTCGTAG207.031745E-445.01
ATCCGTT207.031745E-445.015
CAATGCG253.8895283E-545.01
CCGCGTA207.031745E-445.040
TCGAATA207.031745E-445.044
ATCGAAG253.8895283E-545.043
TACGGCT16150.043.3281757
CGATCGA2050.042.8048841
TGATACC19450.042.5706944
ACGGCTG16500.042.5454528
CGATGAA15800.042.29430419
GATGAAT16550.042.00906420
GATCGAA2000.041.62542
CGAATGC2400.041.25000445
CGTTAGG1150.041.0869562
GATACCT20100.041.082095
CCGATCG2150.040.81395340
CGAATAT1050.040.71428714