SRR3551896_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551896_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411153
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG	11647	2.832765418226305	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC	10518	2.5581717754704454	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC	9913	2.411024606411725	No Hit
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	3152	0.7666245898728696	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAGAGTTT	2975	0.723574922230897	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCT	2424	0.5895615500798973	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2285	0.5557541839655797	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTA	2148	0.5224332547737703	No Hit
GAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT	1949	0.47403278098420776	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTC	1682	0.4090934518293676	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCAGAGTT	630	0.1532276305900723	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATG	598	0.14544463982994166	No Hit
CCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	548	0.1332837167672375	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGT	512	0.12452785216209052	No Hit
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	464	0.11285336602189452	TruSeq Adapter, Index 15 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	452	0.10993474448684554	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT	429	0.10434071987800161	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTAT	426	0.10361106449423937	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	35	1.2093915E-7	45.0	4
AATCCGG	20	7.0279953E-4	45.0	3
CTCACGA	20	7.0279953E-4	45.0	24
CTATCCC	25	3.8864207E-5	45.0	34
CCTAGAT	20	7.0279953E-4	45.0	36
CCGGCTA	20	7.0279953E-4	45.0	30
CGGTTAT	20	7.0279953E-4	45.0	31
GGATCGA	20	7.0279953E-4	45.0	9
TTCGTGC	20	7.0279953E-4	45.0	40
CTAAGCG	25	3.8864207E-5	45.0	1
CACGACG	20	7.0279953E-4	45.0	26
ACGGGTA	35	1.2093915E-7	45.0	5
ATCCGTA	20	7.0279953E-4	45.0	43
ATCCGGC	35	1.2093915E-7	45.0	6
TATTGAG	20	7.0279953E-4	45.0	1
ACGATCG	20	7.0279953E-4	45.0	40
CGGATGG	35	1.2093915E-7	45.0	2
CGTAGGC	25	3.8864207E-5	45.0	35
CGGGCTA	35	1.2093915E-7	45.0	6
AAACCGG	20	7.0279953E-4	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content