##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551896_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411153
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.489927107427164	28.0	25.0	31.0	16.0	33.0
2	27.646027148044645	30.0	25.0	33.0	16.0	33.0
3	27.54058221635255	30.0	25.0	33.0	16.0	34.0
4	31.909911881951487	35.0	30.0	35.0	19.0	37.0
5	33.28043331801057	35.0	32.0	35.0	28.0	37.0
6	33.065381986754325	35.0	32.0	35.0	28.0	37.0
7	33.954360055745674	35.0	33.0	36.0	31.0	37.0
8	33.714174528703424	35.0	33.0	37.0	28.0	37.0
9	35.91277942760967	39.0	35.0	39.0	30.0	39.0
10	35.129630575479204	37.0	34.0	39.0	30.0	39.0
11	35.76622814378103	37.0	35.0	39.0	31.0	39.0
12	35.73076202776095	37.0	35.0	39.0	31.0	39.0
13	35.62597378591425	37.0	35.0	39.0	30.0	39.0
14	36.57736171206339	38.0	35.0	40.0	31.0	41.0
15	36.74090423759525	38.0	35.0	40.0	31.0	41.0
16	36.48593346029337	38.0	34.0	40.0	31.0	41.0
17	36.34946114949909	38.0	35.0	40.0	31.0	41.0
18	36.32371647537535	38.0	35.0	40.0	30.0	41.0
19	36.01823408804022	37.0	34.0	40.0	30.0	41.0
20	35.895411197291516	38.0	34.0	40.0	30.0	41.0
21	36.37051413950525	38.0	35.0	40.0	31.0	41.0
22	36.68392544867726	39.0	35.0	40.0	31.0	41.0
23	36.9478101825841	39.0	35.0	40.0	31.0	41.0
24	36.950991480057304	39.0	35.0	40.0	31.0	41.0
25	36.2659131758737	38.0	35.0	40.0	30.0	41.0
26	36.35675770333671	38.0	35.0	40.0	30.0	41.0
27	36.43641418158204	38.0	35.0	40.0	30.0	41.0
28	36.376541092975124	39.0	35.0	40.0	30.0	41.0
29	36.519835681607574	39.0	35.0	40.0	30.0	41.0
30	36.16324336682452	38.0	35.0	40.0	30.0	41.0
31	36.408988867891026	38.0	35.0	40.0	30.0	41.0
32	36.08364769319451	38.0	35.0	40.0	30.0	41.0
33	36.021580774067075	39.0	35.0	40.0	29.0	41.0
34	36.03084253307163	39.0	35.0	40.0	29.0	41.0
35	35.772439943281455	39.0	35.0	40.0	27.0	41.0
36	35.72873845016332	39.0	35.0	40.0	26.0	41.0
37	35.94566742794045	39.0	35.0	40.0	29.0	41.0
38	35.60548749492281	38.0	35.0	40.0	27.0	41.0
39	35.60036288194419	39.0	35.0	40.0	27.0	41.0
40	35.28902136187745	38.0	35.0	40.0	25.0	41.0
41	35.21313233759695	38.0	34.0	40.0	25.0	41.0
42	35.245650645866625	38.0	35.0	40.0	25.0	41.0
43	35.383652800782194	38.0	35.0	40.0	27.0	41.0
44	35.74651285531177	38.0	35.0	40.0	29.0	41.0
45	35.72220073792481	38.0	35.0	40.0	28.0	41.0
46	35.565266457985224	38.0	35.0	40.0	28.0	41.0
47	35.40903507939867	38.0	35.0	40.0	27.0	41.0
48	35.5011443428602	38.0	35.0	40.0	28.0	41.0
49	35.51654736801142	38.0	35.0	40.0	28.0	41.0
50	35.34733298796312	38.0	35.0	40.0	27.0	41.0
51	34.56100283835944	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	14.0
10	22.0
11	19.0
12	21.0
13	22.0
14	28.0
15	50.0
16	74.0
17	124.0
18	264.0
19	682.0
20	1406.0
21	2184.0
22	3020.0
23	3608.0
24	4492.0
25	5078.0
26	5597.0
27	5556.0
28	6230.0
29	7028.0
30	9106.0
31	12324.0
32	17149.0
33	26436.0
34	35180.0
35	35624.0
36	48537.0
37	73313.0
38	83460.0
39	24500.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.50953537977346	15.876571495282779	17.799213431496305	14.814679693447452
2	28.559198157376937	34.50661919042303	19.9820991212517	16.952083530948332
3	28.51833745588625	32.92472631842647	23.79284597218067	14.764090253506602
4	25.832475988257414	20.62906022818756	36.614350375650915	16.924113407904112
5	33.68940516060931	24.189535282486084	22.572618952068936	19.54844060483567
6	22.24159862630213	40.71890512777482	23.449907941812416	13.589588304110636
7	66.87753707257396	5.576269661172361	22.575537573603988	4.970655692649695
8	64.8522569457112	16.806638891118393	12.081390625874066	6.259713537296335
9	60.671818033676026	7.128246662434666	12.098415918161852	20.101519385727453
10	44.026432982369094	21.385956079610267	20.378545213095855	14.209065724924786
11	35.770625533560505	21.513888990229916	24.674512894226723	18.04097258198286
12	27.862863702806496	18.9576629624495	27.95407062577678	25.22540270896722
13	27.14926073748702	23.27333133894195	32.955858281467	16.621549642104032
14	20.033418216576308	32.66934693410969	25.477377034826453	21.819857814487552
15	16.056796375072054	24.365382229972784	37.189805254978076	22.388016139977086
16	18.661179658180775	23.58951533857226	29.439892205577973	28.309412797668994
17	18.474144661476384	27.930721653496388	30.662308191840992	22.932825493186236
18	18.84383672258259	24.648245300411283	30.3111007337901	26.19681724321603
19	21.729623765362284	26.927688719284546	25.727162394534396	25.615525120818774
20	26.41547063988345	25.20156729976432	31.763601384399482	16.619360675952745
21	22.320644626209706	32.399374442117654	26.618314836569358	18.661666095103282
22	19.793604813779787	23.07413541917486	31.60818478765812	25.524074979387233
23	23.24536121589773	28.97753391073396	27.642264558449043	20.134840314919263
24	22.324049684667266	26.343721193813497	27.90688624429349	23.425342877225752
25	18.495061449144238	33.99026639718061	25.813504948279594	21.701167205395556
26	18.79422015648676	25.53745199475621	31.22073777888037	24.447590069876664
27	24.49501766982121	27.91102095813481	25.357713551889443	22.236247820154542
28	16.792532220365654	30.515890678166034	31.370803569474138	21.32077353199417
29	22.028539254243555	22.627099887389853	30.091717681738917	25.252643176627675
30	18.36956072313713	32.5299827558111	26.857155365520864	22.243301155530908
31	24.251799208567128	26.971711260771535	21.881392085184835	26.8950974454765
32	29.33263286416492	28.311844982281535	23.521900606343625	18.83362154720992
33	24.032172938054693	27.669261807648247	22.627829542773615	25.670735711523445
34	24.821660063285446	25.05077185378679	24.29533531313161	25.83223276979616
35	21.755891359177724	23.366970446524775	28.21796265623746	26.659175538060044
36	20.60060366822083	29.530855910087002	26.40793086758457	23.460609554107595
37	23.257278920499182	24.938648143148654	28.58911402811119	23.21495890824097
38	19.491041047979706	31.377370467927996	24.152079639452953	24.979508844639344
39	27.90007612737837	25.72570308376687	25.592176148538382	20.78204464031638
40	20.917517323234904	26.26005404314209	29.935571429613795	22.886857204009214
41	21.152223138345093	25.09649692450256	28.95831965229489	24.79296028485746
42	21.291830535104936	22.794677407193916	33.15675673046287	22.756735327238278
43	22.73606175803168	23.992528328870275	27.924154755042522	25.347255158055514
44	22.550486072094817	23.299842151218648	29.251641116567313	24.898030660119225
45	20.580902972859253	23.868000476708183	27.620861333858688	27.93023521657388
46	26.520297796683963	27.571244767762852	24.707347386496025	21.201110049057164
47	18.272030120174243	26.498408135171093	33.68454079138423	21.545020953270438
48	23.465230704871423	25.578799133169404	26.544862861270623	24.41110730068855
49	21.266535815134514	21.804048614506037	34.02456020021744	22.904855370142016
50	20.89587088018329	24.364895793050277	30.2427563461777	24.496476980588735
51	20.727563704995465	23.581975566273382	26.778839020997047	28.911621707734103
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	206.0
1	463.5
2	721.0
3	2357.5
4	3994.0
5	2786.0
6	1578.0
7	1594.0
8	1610.0
9	1588.0
10	1566.0
11	1500.5
12	1435.0
13	1352.0
14	1269.0
15	1132.5
16	996.0
17	1044.0
18	1092.0
19	1233.0
20	1374.0
21	1382.0
22	1390.0
23	1412.0
24	1434.0
25	1695.0
26	2480.5
27	3005.0
28	3511.5
29	4018.0
30	5016.5
31	6015.0
32	6217.0
33	6419.0
34	7206.5
35	7994.0
36	9165.0
37	10336.0
38	11053.5
39	11771.0
40	13941.5
41	16112.0
42	18303.5
43	20495.0
44	23054.0
45	25613.0
46	48340.0
47	71067.0
48	53497.0
49	35927.0
50	35362.5
51	34798.0
52	30376.0
53	25954.0
54	23894.0
55	21834.0
56	20628.0
57	19422.0
58	18348.5
59	17275.0
60	16696.0
61	16117.0
62	14147.5
63	12178.0
64	10470.0
65	8762.0
66	6741.0
67	4720.0
68	4177.5
69	3635.0
70	2962.0
71	2289.0
72	2050.5
73	1812.0
74	1583.0
75	1003.5
76	653.0
77	571.5
78	490.0
79	369.0
80	248.0
81	206.0
82	164.0
83	98.5
84	33.0
85	21.5
86	10.0
87	7.5
88	5.0
89	5.5
90	6.0
91	3.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	411153.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.886589264991755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.47379487309954	18.28512154747085
2	10.303534666144447	5.128396704278816
3	3.622726127444362	2.7047189145978603
4	1.7882937577793523	1.7801812894001359
5	1.0988558685938765	1.367338733156078
6	0.7392239370392591	1.1038057497567701
7	0.579391239196417	1.0093350255531075
8	0.4565202997920301	0.9088986553644124
9	0.41756123350234686	0.9352507420040599
>10	6.800853768116729	41.6636554247721
>50	0.6463208560440429	9.735855801308663
>100	0.058938068789178566	2.7033271023806345
>500	0.003995801273842615	0.5688074682956494
>1k	0.006992652229224575	4.130566471036807
>5k	9.989503184606536E-4	2.464418021509953
>10k+	0.0019979006369213073	5.510322349114103
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG	11647	2.832765418226305	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC	10518	2.5581717754704454	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC	9913	2.411024606411725	No Hit
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	3152	0.7666245898728696	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAGAGTTT	2975	0.723574922230897	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCT	2424	0.5895615500798973	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2285	0.5557541839655797	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTA	2148	0.5224332547737703	No Hit
GAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT	1949	0.47403278098420776	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTC	1682	0.4090934518293676	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCAGAGTT	630	0.1532276305900723	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATG	598	0.14544463982994166	No Hit
CCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	548	0.1332837167672375	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGT	512	0.12452785216209052	No Hit
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	464	0.11285336602189452	TruSeq Adapter, Index 15 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	452	0.10993474448684554	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT	429	0.10434071987800161	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTAT	426	0.10361106449423937	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11820417216948435	0.0
2	0.0	0.0	0.0	1.1611249340269925	0.0
3	0.0	0.0	0.0	1.3369718815136944	0.0
4	0.0	0.0	0.0	2.062735769895878	0.0
5	0.0	0.0	0.0	5.254005200010702	0.0
6	0.0	0.0	0.0	5.723173611769828	0.0
7	0.0	0.0	0.0	6.688994121409792	0.0
8	0.0	0.0	0.0	7.62295301262547	0.0
9	0.0	0.0	0.0	7.8401470985253665	0.0
10	0.0	0.0	0.0	11.233531069942332	0.0
11	0.0	0.0	0.0	11.990183702903785	0.0
12	0.0	0.0	0.0	15.257580511391136	0.0
13	0.0	0.0	0.0	15.64016315094381	0.0
14	0.0	0.0	0.0	15.848114935316051	0.0
15	0.0	0.0	0.0	16.601119291358692	0.0
16	0.0	0.0	0.0	17.08828586925062	0.0
17	0.0	0.0	0.0	17.584208311747695	0.0
18	0.0	0.0	0.0	18.055565689658106	0.0
19	0.0	0.0	0.0	19.16780371297303	0.0
20	0.0	0.0	0.0	19.597813952470247	0.0
21	0.0	0.0	0.0	19.9582637120488	0.0
22	0.0	0.0	0.0	20.445916726863235	0.0
23	0.0	0.0	0.0	20.839930634094852	0.0
24	0.0	0.0	0.0	21.172410270629182	0.0
25	0.0	0.0	0.0	21.444814947233755	0.0
26	0.0	0.0	0.0	21.707490885388165	0.0
27	0.0	0.0	0.0	22.085938811099517	0.0
28	0.0	0.0	0.0	22.36126210923914	0.0
29	0.0	0.0	0.0	22.648746330441465	0.0
30	0.0	0.0	0.0	22.95690412085039	0.0
31	0.0	0.0	0.0	23.25776535742169	0.0
32	0.0	0.0	0.0	23.570787517055695	0.0
33	0.0	0.0	0.0	23.880647836693395	0.0
34	0.0	0.0	0.0	24.194642870172416	0.0
35	0.0	0.0	0.0	24.531500439009324	0.0
36	0.0	0.0	0.0	24.84525225402709	0.0
37	0.0	0.0	0.0	25.170192118262545	0.0
38	0.0	0.0	0.0	25.469107607143812	0.0
39	0.0	0.0	0.0	25.77142815448264	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	35	1.2093915E-7	45.0	4
AATCCGG	20	7.0279953E-4	45.0	3
CTCACGA	20	7.0279953E-4	45.0	24
CTATCCC	25	3.8864207E-5	45.0	34
CCTAGAT	20	7.0279953E-4	45.0	36
CCGGCTA	20	7.0279953E-4	45.0	30
CGGTTAT	20	7.0279953E-4	45.0	31
GGATCGA	20	7.0279953E-4	45.0	9
TTCGTGC	20	7.0279953E-4	45.0	40
CTAAGCG	25	3.8864207E-5	45.0	1
CACGACG	20	7.0279953E-4	45.0	26
ACGGGTA	35	1.2093915E-7	45.0	5
ATCCGTA	20	7.0279953E-4	45.0	43
ATCCGGC	35	1.2093915E-7	45.0	6
TATTGAG	20	7.0279953E-4	45.0	1
ACGATCG	20	7.0279953E-4	45.0	40
CGGATGG	35	1.2093915E-7	45.0	2
CGTAGGC	25	3.8864207E-5	45.0	35
CGGGCTA	35	1.2093915E-7	45.0	6
AAACCGG	20	7.0279953E-4	45.0	2
>>END_MODULE