##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551894_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 430717 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.295936775191134 28.0 25.0 31.0 16.0 33.0 2 27.71438786952918 30.0 25.0 33.0 16.0 34.0 3 27.517042512833253 30.0 25.0 33.0 16.0 34.0 4 31.73418044795074 35.0 28.0 35.0 19.0 37.0 5 33.22450239948736 35.0 32.0 35.0 28.0 37.0 6 32.936519803026115 35.0 32.0 35.0 28.0 37.0 7 33.998393376625486 35.0 33.0 37.0 31.0 37.0 8 33.879296614714534 35.0 35.0 37.0 30.0 37.0 9 36.125734531026175 39.0 35.0 39.0 31.0 39.0 10 35.28183702988273 37.0 34.0 39.0 31.0 39.0 11 35.817622708181936 37.0 35.0 39.0 31.0 39.0 12 35.82049466354938 37.0 35.0 39.0 31.0 39.0 13 35.83458047859732 37.0 35.0 39.0 31.0 39.0 14 36.70274449348412 38.0 36.0 40.0 31.0 41.0 15 36.97688969787587 39.0 35.0 40.0 32.0 41.0 16 36.57424248404405 38.0 34.0 40.0 31.0 41.0 17 36.63150281971689 38.0 35.0 40.0 31.0 41.0 18 36.5253612000455 38.0 35.0 40.0 31.0 41.0 19 36.16092004727002 38.0 34.0 40.0 30.0 41.0 20 36.34177894069656 38.0 35.0 40.0 31.0 41.0 21 36.622123110998636 38.0 35.0 40.0 31.0 41.0 22 36.964271203597725 39.0 35.0 40.0 32.0 41.0 23 37.264554220056326 39.0 35.0 40.0 32.0 41.0 24 37.2898144257134 39.0 35.0 41.0 32.0 41.0 25 36.385317969803836 38.0 35.0 40.0 30.0 41.0 26 36.62115727960587 38.0 35.0 40.0 31.0 41.0 27 36.819677421601654 39.0 35.0 40.0 31.0 41.0 28 36.799808226747494 39.0 35.0 40.0 31.0 41.0 29 36.902314048435514 39.0 35.0 40.0 31.0 41.0 30 36.52473201661416 39.0 35.0 40.0 30.0 41.0 31 36.70913151791083 39.0 35.0 40.0 31.0 41.0 32 36.558187858849315 39.0 35.0 40.0 31.0 41.0 33 36.605228026755384 39.0 35.0 40.0 31.0 41.0 34 36.61843623539354 39.0 35.0 41.0 31.0 41.0 35 36.52684709449592 39.0 35.0 41.0 31.0 41.0 36 36.48064042050813 39.0 35.0 41.0 30.0 41.0 37 36.56496493056926 39.0 35.0 41.0 31.0 41.0 38 36.37658833990764 39.0 35.0 41.0 30.0 41.0 39 36.313005987690296 39.0 35.0 40.0 30.0 41.0 40 36.23601343805794 39.0 35.0 40.0 30.0 41.0 41 35.937866394871804 39.0 35.0 40.0 29.0 41.0 42 36.07851559144403 39.0 35.0 40.0 30.0 41.0 43 36.10901125332875 39.0 35.0 40.0 30.0 41.0 44 36.209436822786195 39.0 35.0 40.0 30.0 41.0 45 36.20227434719317 39.0 35.0 40.0 30.0 41.0 46 35.99003986376205 39.0 35.0 40.0 30.0 41.0 47 35.87177659576938 38.0 35.0 40.0 29.0 41.0 48 35.94629652416784 38.0 35.0 40.0 30.0 41.0 49 35.948323377066615 38.0 35.0 40.0 30.0 41.0 50 35.822769939426585 38.0 35.0 40.0 30.0 41.0 51 34.87970987910855 37.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 9.0 9 15.0 10 22.0 11 24.0 12 26.0 13 27.0 14 38.0 15 48.0 16 73.0 17 104.0 18 245.0 19 446.0 20 939.0 21 1694.0 22 2329.0 23 2890.0 24 3667.0 25 4406.0 26 4879.0 27 5038.0 28 5554.0 29 6678.0 30 8655.0 31 11789.0 32 17208.0 33 27076.0 34 35653.0 35 35969.0 36 51467.0 37 79294.0 38 95285.0 39 29169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.647062920664844 17.032297308905846 19.683690218867607 15.636949551561699 2 31.406468748621485 28.588609226011513 21.642981354346357 18.361940671020648 3 30.74106664004439 27.686624860407182 24.935398417058067 16.63691008249036 4 27.337439664559327 21.98659444600515 31.22142845534307 19.454537434092455 5 29.280478829486643 26.576150929728804 23.899219208900508 20.244151031884044 6 24.288802160119058 37.51349493983288 23.98465813979945 14.213044760248609 7 72.01294585539925 6.601782608998484 16.518038526457048 4.867233009145216 8 71.26721257809653 11.995811635017889 10.925735459710204 5.811240327175384 9 65.80144271064296 8.401340091057468 11.842578769818697 13.95463842848088 10 42.60871987871386 23.13909132910937 19.530689524676294 14.721499267500471 11 34.3211435815164 23.84930244220683 23.68446102661376 18.145092949663002 12 28.761808797888172 21.52364545629729 28.19600805169055 21.518537694123985 13 25.861528567481663 24.236331512338726 31.539967078151083 18.362172842028524 14 20.201199395426695 31.082358021624408 26.814590554819056 21.901852028129838 15 17.872292015406867 25.415992403364623 36.80537336580632 19.90634221542219 16 20.304283322924334 24.745482532614222 30.232379961784652 24.717854182676792 17 20.797414543656277 26.44404562624647 29.644290798830784 23.11424903126647 18 20.941360568540365 26.191907911691438 29.644522969838665 23.222208549929533 19 24.00555353050843 26.999398677089598 25.94371710427032 23.05133068813165 20 25.99433038398763 25.947431840396362 29.976759682111453 18.08147809350455 21 23.771989496583604 28.72001801647021 27.158667988493608 20.34932449845258 22 21.77996224899412 24.33036077052914 29.389367032181223 24.500309948295516 23 22.74625798378053 28.387316962181664 28.088745046051116 20.77768000798668 24 22.068782982793806 25.836454098630888 28.348312232858238 23.746450685717072 25 20.766071457592805 30.201036875721183 27.629046450453547 21.40384521623247 26 20.855689466633542 26.376948204969853 29.57881857460932 23.18854375378729 27 23.664029977920535 27.693125648627753 27.192332784635852 21.450511588815857 28 19.005983046873006 27.31375822175582 32.356048170840715 21.324210560530467 29 23.209903486512026 24.7953992993079 29.068738870302308 22.925958343877767 30 20.70268877244223 28.726286633682907 27.79179832697572 22.779226266899148 31 23.619220973400168 25.94673532737273 25.824613377229134 24.609430321997973 32 25.543454286689403 27.80387121938535 24.911716974254556 21.74095751967069 33 22.32835016960092 26.16497607477764 25.0178191248546 26.488854630766838 34 23.698623458094296 26.368125706670504 26.580330007870597 23.352920827364603 35 21.590975048581782 25.196590800920326 27.17724166912381 26.035192481374082 36 23.073154762872143 29.894803316330677 26.069089448524206 20.962952472272978 37 20.756784617277702 26.954589672569227 27.82267707102343 24.465948639129635 38 20.130619409031915 27.043975510602092 27.02470531694825 25.800699763417747 39 22.7455614707569 28.27285665529803 25.480768114562462 23.50081375938261 40 21.744207913780976 24.43901680221584 29.780343009447037 24.036432274556148 41 19.175932224639382 29.209434501076114 26.906065931922818 24.70856734236169 42 20.628626220929288 25.168498108967142 30.46896221881189 23.733913451291684 43 22.83053605964009 27.14961331918638 25.876155340977952 24.143695280195583 44 21.350910226436383 25.461033578892867 28.76784524409299 24.420210950577758 45 21.321424508435936 25.372112082875763 26.977574602349108 26.328888806339197 46 24.132086729801703 28.14330523290235 25.38116675218299 22.343441285112963 47 20.125975988874366 25.656289396517902 31.947195025968327 22.27053958863941 48 21.277312016939195 26.27665032956675 28.139126154760554 24.30691149873351 49 20.745872579907456 24.381670563270085 31.754028747414196 23.118428109408264 50 20.798343227687784 25.17708843625861 29.364292563330448 24.660275772723157 51 20.930448531170118 25.611944734013285 26.43499095693924 27.022615777877352 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 238.0 1 605.5 2 973.0 3 2252.5 4 3532.0 5 2563.5 6 1595.0 7 1615.0 8 1635.0 9 1591.5 10 1548.0 11 1563.5 12 1579.0 13 1525.5 14 1472.0 15 1451.5 16 1431.0 17 1411.5 18 1392.0 19 1351.0 20 1310.0 21 1379.0 22 1448.0 23 1429.0 24 1410.0 25 1949.0 26 3066.0 27 3644.0 28 4322.0 29 5000.0 30 6094.0 31 7188.0 32 7344.0 33 7500.0 34 8665.0 35 9830.0 36 10341.0 37 10852.0 38 12197.0 39 13542.0 40 15432.0 41 17322.0 42 20121.5 43 22921.0 44 24791.5 45 26662.0 46 41409.0 47 56156.0 48 46804.5 49 37453.0 50 36757.0 51 36061.0 52 32151.5 53 28242.0 54 27020.5 55 25799.0 56 23893.5 57 21988.0 58 20424.0 59 18860.0 60 18101.0 61 17342.0 62 15639.0 63 13936.0 64 11873.5 65 9811.0 66 8289.0 67 6767.0 68 5510.5 69 4254.0 70 3490.5 71 2727.0 72 2414.0 73 2101.0 74 1661.0 75 979.5 76 738.0 77 539.5 78 341.0 79 272.0 80 203.0 81 164.0 82 125.0 83 81.5 84 38.0 85 28.0 86 18.0 87 18.0 88 18.0 89 12.0 90 6.0 91 3.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 430717.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.931635055984977 #Duplication Level Percentage of deduplicated Percentage of total 1 75.67163986810459 20.37960989015716 2 9.354188247396063 5.03847168247709 3 3.211823307763572 2.594989595669851 4 1.5546052654547562 1.6747224666136058 5 0.9952680695324935 1.3402098215761897 6 0.6262753503985424 1.0119971508895595 7 0.534696521562659 1.0080176109101093 8 0.4120341609080808 0.8877402921740339 9 0.357614284649396 0.8668023664486195 >10 6.501841284176574 43.45503361817121 >50 0.7186836639250657 11.872584981363495 >100 0.05182814884074993 2.5913338710180853 >500 8.638024806791655E-4 0.1267866917770766 >1k 0.006046617364754158 2.4636166347142043 >5k 0.0025914074420374964 4.68808332603972 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGC 6888 1.599193902260649 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCG 6802 1.579227195583179 No Hit GAATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC 6462 1.5002890529048076 No Hit GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC 2012 0.4671280678496553 RNA PCR Primer, Index 14 (95% over 24bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCATAT 1880 0.4364814948098171 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1839 0.426962483486837 No Hit GAATGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT 1387 0.3220211879261789 No Hit GAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT 1280 0.29717889008327975 RNA PCR Primer, Index 14 (95% over 22bp) GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTA 1137 0.2639784359567883 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTC 1055 0.24494041331082822 No Hit CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC 545 0.12653319929327145 Illumina PCR Primer Index 7 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.321710078775623E-4 0.0 0.0 0.08938583803286149 0.0 2 2.321710078775623E-4 0.0 0.0 0.7793980734449766 0.0 3 2.321710078775623E-4 0.0 0.0 0.9228797563133101 0.0 4 2.321710078775623E-4 0.0 0.0 1.4276195274391306 0.0 5 2.321710078775623E-4 0.0 0.0 3.548037342384907 0.0 6 2.321710078775623E-4 0.0 0.0 3.9589800263281925 0.0 7 2.321710078775623E-4 0.0 0.0 4.634365488244021 0.0 8 2.321710078775623E-4 0.0 0.0 5.381259620586139 0.0 9 2.321710078775623E-4 0.0 0.0 5.589981356668067 0.0 10 4.643420157551246E-4 0.0 0.0 7.733151930385845 0.0 11 4.643420157551246E-4 0.0 0.0 8.487707705987923 0.0 12 4.643420157551246E-4 0.0 0.0 10.749285493723256 0.0 13 4.643420157551246E-4 0.0 0.0 11.096845492515968 0.0 14 4.643420157551246E-4 0.0 0.0 11.252864409809689 0.0 15 4.643420157551246E-4 0.0 0.0 11.730208002005957 0.0 16 4.643420157551246E-4 0.0 0.0 12.187584887524755 0.0 17 4.643420157551246E-4 0.0 0.0 12.645658286067185 0.0 18 4.643420157551246E-4 0.0 0.0 13.169436079838967 0.0 19 4.643420157551246E-4 0.0 0.0 13.906811200858105 0.0 20 4.643420157551246E-4 0.0 0.0 14.29291158695849 0.0 21 4.643420157551246E-4 0.0 0.0 14.651383623121447 0.0 22 4.643420157551246E-4 0.0 0.0 15.114564783837183 0.0 23 4.643420157551246E-4 0.0 0.0 15.4920748426461 0.0 24 4.643420157551246E-4 0.0 0.0 15.808756097391095 0.0 25 4.643420157551246E-4 0.0 0.0 16.1036132773956 0.0 26 4.643420157551246E-4 0.0 0.0 16.375949869635978 0.0 27 4.643420157551246E-4 0.0 0.0 16.724206381452323 0.0 28 4.643420157551246E-4 0.0 0.0 16.98748830438548 0.0 29 4.643420157551246E-4 0.0 0.0 17.270272591980348 0.0 30 4.643420157551246E-4 0.0 0.0 17.5874181887411 0.0 31 4.643420157551246E-4 0.0 0.0 17.892490893092216 0.0 32 4.643420157551246E-4 0.0 0.0 18.20731477977419 0.0 33 4.643420157551246E-4 0.0 0.0 18.49961807869204 0.0 34 4.643420157551246E-4 0.0 0.0 18.781009340239645 0.0 35 4.643420157551246E-4 0.0 0.0 19.096529739945254 0.0 36 4.643420157551246E-4 0.0 0.0 19.401138102280616 0.0 37 4.643420157551246E-4 0.0 0.0 19.687172783985773 0.0 38 4.643420157551246E-4 0.0 0.0 19.98922726523448 0.0 39 4.643420157551246E-4 0.0 0.0 20.288727865396538 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTGT 30 2.162329E-6 45.000004 10 TAGTGCG 30 2.162329E-6 45.000004 1 CGCAAGT 30 2.162329E-6 45.000004 23 TAATGCG 35 1.2095552E-7 45.000004 1 TGCACGG 60 0.0 45.000004 2 TCGCCTT 20 7.028369E-4 45.0 27 GGTCGTT 20 7.028369E-4 45.0 8 CGACGGT 20 7.028369E-4 45.0 28 ATAATGC 20 7.028369E-4 45.0 18 CGTCTAG 20 7.028369E-4 45.0 25 TAACGCG 25 3.8867303E-5 45.0 1 CATTCCG 20 7.028369E-4 45.0 26 CGTTCGG 20 7.028369E-4 45.0 2 CTAACCG 20 7.028369E-4 45.0 1 CTACGAA 50 2.1827873E-11 45.0 11 GCCTCGA 25 3.8867303E-5 45.0 14 CGAATAT 50 2.1827873E-11 45.0 14 GGACGTA 20 7.028369E-4 45.0 8 CGTAAGG 55 1.8189894E-12 45.0 2 CGGCTAT 25 3.8867303E-5 45.0 31 >>END_MODULE