FastQCFastQC Report
Sat 18 Jun 2016
SRR3551893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430669
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG105452.4485161458103555No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC104322.4222778978751665No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC88332.05099507974802No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41700.9682610078737963No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAAGCCAT35010.812921292222101No Hit
GCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC27160.6306467379820698No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT21090.4897032291620711No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTA19120.4439604429387766No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCACCCATCGAATGC18180.4221339358068493No Hit
GAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT15690.36431691159568025No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTC13530.3141623845691238No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGAAGCCA6460.14999918731090467No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGAACCCATCGAATGC6150.1428010838950563No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATG5780.1342097991729147No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC5770.1339776022885325No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCATCGAATGC5750.13351320851976808No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGT4790.11122230761907637No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT4510.1047207948563746No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT4470.1037920073188458No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGA207.028367E-445.00000412
GATACCC406.7975634E-945.00000435
AGTCATA253.8867285E-545.00000435
ATAGGTA207.028367E-445.00000416
CGAAAGG302.1623273E-645.0000042
GCGCGAC207.028367E-445.0000049
CACGCTA207.028367E-445.00000441
GGCCCGT207.028367E-445.00000438
ATCCGGC406.7975634E-945.0000046
CACTATA253.8867285E-545.00000416
TCGTCAC406.7975634E-945.00000412
GTCAAGT207.028367E-445.00000440
CGGGATA253.8867285E-545.0000046
ACGCTAA207.028367E-445.00000442
CGTACAC302.1623273E-645.00000413
CGTAAGG600.045.0000042
ACGTAAG207.028367E-445.0000041
CCAACGG302.1623273E-645.0000042
GGTTCGA207.028367E-445.00000438
CCACGCA302.1623273E-645.00000441
Produced by FastQC (version 0.11.5)