##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551893_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	430669
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.58963844623133	30.0	25.0	31.0	16.0	33.0
2	27.632666850876195	30.0	25.0	33.0	16.0	33.0
3	27.58386835365443	30.0	25.0	33.0	16.0	34.0
4	31.838031063299194	35.0	30.0	35.0	19.0	37.0
5	33.26060152924868	35.0	32.0	35.0	28.0	37.0
6	33.08289660969329	35.0	32.0	35.0	28.0	37.0
7	33.89155708908698	35.0	33.0	36.0	30.0	37.0
8	33.672258741632206	35.0	33.0	37.0	28.0	37.0
9	35.79596395375567	38.0	35.0	39.0	30.0	39.0
10	35.132932716308815	37.0	34.0	39.0	30.0	39.0
11	35.818110892588045	37.0	35.0	39.0	31.0	39.0
12	35.752146079703905	37.0	35.0	39.0	31.0	39.0
13	35.62424506987965	37.0	35.0	39.0	30.0	39.0
14	36.61282330513689	38.0	35.0	40.0	31.0	41.0
15	36.71565633932324	39.0	35.0	40.0	31.0	41.0
16	36.49288200450926	38.0	34.0	40.0	31.0	41.0
17	36.242176706472954	38.0	35.0	40.0	30.0	41.0
18	36.32945487137454	38.0	35.0	40.0	30.0	41.0
19	35.80860243017259	37.0	34.0	40.0	29.0	41.0
20	35.45712136234556	37.0	34.0	40.0	27.0	41.0
21	36.18682096923624	38.0	35.0	40.0	30.0	41.0
22	36.44993486877393	38.0	35.0	40.0	31.0	41.0
23	36.57168266116205	38.0	35.0	40.0	30.0	41.0
24	36.797066888956486	39.0	35.0	40.0	31.0	41.0
25	36.11301254559766	38.0	35.0	40.0	30.0	41.0
26	36.030719647803764	38.0	35.0	40.0	30.0	41.0
27	36.049581000722135	38.0	35.0	40.0	30.0	41.0
28	36.078628830958344	38.0	35.0	40.0	29.0	41.0
29	36.25064260487753	38.0	35.0	40.0	30.0	41.0
30	35.73797974778775	38.0	35.0	40.0	27.0	41.0
31	35.81180906914591	38.0	35.0	40.0	28.0	41.0
32	35.3790428380032	38.0	35.0	40.0	25.0	41.0
33	35.429538694449796	38.0	35.0	40.0	25.0	41.0
34	35.28451084243352	39.0	35.0	40.0	24.0	41.0
35	35.059419182713405	39.0	35.0	40.0	23.0	41.0
36	35.01502546038837	39.0	35.0	40.0	23.0	41.0
37	34.74999825852337	39.0	35.0	40.0	19.0	41.0
38	34.611211858759276	38.0	34.0	40.0	20.0	41.0
39	34.64221478676199	38.0	34.0	40.0	18.0	41.0
40	34.530627930034434	38.0	34.0	40.0	21.0	41.0
41	34.63666992516294	38.0	34.0	40.0	22.0	41.0
42	34.640296840496994	38.0	34.0	40.0	22.0	41.0
43	34.55491572414081	38.0	34.0	40.0	20.0	41.0
44	34.72326775319329	38.0	35.0	40.0	20.0	41.0
45	34.71943882656983	38.0	35.0	40.0	20.0	41.0
46	34.519359415235364	38.0	34.0	40.0	20.0	41.0
47	34.271951312957285	38.0	34.0	40.0	20.0	41.0
48	34.43179797013484	38.0	34.0	40.0	20.0	41.0
49	34.435039438640814	38.0	34.0	40.0	20.0	41.0
50	34.219597881435625	37.0	34.0	40.0	20.0	41.0
51	33.43758431649364	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	14.0
10	25.0
11	17.0
12	18.0
13	22.0
14	36.0
15	51.0
16	83.0
17	124.0
18	313.0
19	1085.0
20	2520.0
21	4633.0
22	6146.0
23	6513.0
24	6477.0
25	6914.0
26	7299.0
27	7049.0
28	6896.0
29	7441.0
30	9324.0
31	12301.0
32	17356.0
33	25508.0
34	34194.0
35	35872.0
36	47755.0
37	71902.0
38	85567.0
39	27209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.37534626360384	15.720890057097215	17.67343365786718	16.230330021431772
2	28.60456638392826	35.78037889887594	19.81591431006179	15.799140407134015
3	27.30913996595994	34.33193473409974	25.059152156296367	13.299773143643959
4	25.05195405288052	20.083869514638852	38.96379818375597	15.900378248724659
5	35.15669806742533	23.400802008038657	23.008853667201492	18.43364625733452
6	21.21002440389255	42.30139619986579	23.766976494709393	12.721602901532266
7	64.61992852979898	5.625898311696453	25.42880959623284	4.325363562271722
8	63.814205340992736	18.93913887463458	12.051714890089604	5.19494089428308
9	58.6982113873996	7.766753585700386	12.363323108930524	21.171711917969485
10	44.37166362101753	20.763045401456804	20.54152957375618	14.323761403769483
11	37.35026203418402	20.92070708595232	24.542978482314727	17.186052397548927
12	26.333913051554674	18.731090466228125	27.990173427852945	26.94482305436426
13	29.455335768304664	21.37627737311021	31.70230501847126	17.46608184011387
14	18.393243999452018	33.67992588275451	26.701480719531705	21.225349398261777
15	16.16159974365464	22.5038254436702	36.38641276711349	24.948162045561673
16	18.690223814576857	22.49802052156064	28.62894705678839	30.182808607074108
17	18.73248364753442	29.62019555621603	30.233195330985048	21.414125465264508
18	18.876213518967003	24.352344840236935	29.016948050591058	27.75449359020501
19	20.864283243047442	24.915886678632546	26.274935042921594	27.944895035398414
20	28.260450601273835	23.27796985620047	32.058959432882325	16.40262010964337
21	22.337340277568156	32.81777885104338	27.467730437992984	17.377150433395485
22	19.9097218513522	21.844386292024733	34.80166903120494	23.44422282541813
23	25.447153150099034	26.55171372910518	29.143495352579357	18.857637768216424
24	20.916991935802205	28.61408645618793	27.891025358221743	22.57789624978812
25	18.617081795996462	35.067302266938185	26.588633033721955	19.726982903343405
26	18.366076963979296	23.97897225015035	34.92821633319324	22.726734452677114
27	27.947913594895386	25.904581012331978	26.18948658946894	19.9580188033037
28	17.380633386661216	31.318019174818712	31.85927011231363	19.442077326206437
29	21.35120010959693	23.13981270999306	34.70112778026744	20.80785940014257
30	21.30313535452981	32.31205403685893	28.08769611929347	18.29711448931778
31	22.172248292772405	25.762476519090065	24.147314991327445	27.91796019681008
32	28.90967309000648	28.744116711441965	24.09390970791954	18.252300490632017
33	27.92446170957278	24.95512795209314	23.123791124970687	23.996619213363395
34	21.62194167678658	24.395765657616405	26.104734726669438	27.877557938927577
35	27.630964847713674	23.298635378910486	24.50466599639166	24.56573377698418
36	20.678061341772917	34.765678514125696	25.009229826154193	19.547030317947193
37	23.18648428375388	28.078408243918183	27.252251729286296	21.48285574304164
38	22.705836733082716	28.625464103522656	22.80103745567942	25.867661707715207
39	21.2197766730366	30.466553199789164	24.57177089597812	23.741899231196115
40	22.85699690481553	22.929210135858398	26.975008649333944	27.23878430999213
41	19.110267978424268	27.317731250682076	24.739417046502073	28.832583724391586
42	19.768313948763435	24.545764844927312	28.704178847328226	26.981742358981027
43	21.757776854150173	28.953558301154715	25.33198349544546	23.956681349249656
44	23.243604717311904	23.194146780938492	29.81222237960011	23.750026122149492
45	20.717070418349127	24.317747504463984	26.4184327174698	28.546749359717094
46	26.197613480422323	28.92430149372256	24.46542472293107	20.412660302924053
47	19.56792803754159	28.103021113662695	32.752531526531975	19.57651932226373
48	21.299188007495314	27.95673707650191	26.08755215722515	24.656522758777623
49	21.40414099923607	23.715428786376545	31.79146862207403	23.088961592313353
50	22.002744567173398	26.012552563569702	28.340790723270075	23.643912145986825
51	20.310261476911503	23.402195189344948	28.490325516812216	27.79721781693133
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	242.0
1	565.0
2	888.0
3	3947.5
4	7007.0
5	4736.0
6	2465.0
7	2478.5
8	2492.0
9	2405.0
10	2318.0
11	2235.0
12	2152.0
13	1959.0
14	1766.0
15	1649.0
16	1532.0
17	1448.0
18	1364.0
19	1345.5
20	1327.0
21	1430.5
22	1534.0
23	1453.5
24	1373.0
25	1652.5
26	2509.0
27	3086.0
28	3333.0
29	3580.0
30	4918.5
31	6257.0
32	7041.0
33	7825.0
34	8821.5
35	9818.0
36	10258.5
37	10699.0
38	11095.5
39	11492.0
40	13778.5
41	16065.0
42	17965.5
43	19866.0
44	21407.5
45	22949.0
46	44089.5
47	65230.0
48	50036.5
49	34843.0
50	33528.0
51	32213.0
52	31164.0
53	30115.0
54	29407.0
55	28699.0
56	25387.0
57	22075.0
58	20310.0
59	18545.0
60	17891.5
61	17238.0
62	15307.5
63	13377.0
64	11676.0
65	9975.0
66	8217.5
67	6460.0
68	5481.0
69	4502.0
70	3563.5
71	2625.0
72	2225.5
73	1826.0
74	1575.5
75	1100.0
76	875.0
77	621.5
78	368.0
79	294.0
80	220.0
81	152.5
82	85.0
83	56.0
84	27.0
85	19.0
86	11.0
87	6.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	430669.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.051711301307186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.73937984581164	17.49506564287584
2	9.669554300935312	4.651386569168188
3	3.463760216031195	2.499280821988071
4	1.8536512941969852	1.7833394312528128
5	1.2362293045803918	1.4866715167991666
6	0.8650047937821275	1.248290734457669
7	0.6704469738267321	1.128777794012093
8	0.6215593000409527	1.1959651872982053
9	0.540746494483121	1.1705290715251733
>10	7.616344241711627	42.02338380343703
>50	0.6345290720076837	9.45829685877536
>100	0.07283116707006432	3.393286378119099
>500	0.004988436100689337	0.7177229019838925
>1k	0.00798149776110294	4.5947703534562265
>5k	9.976872201378675E-4	2.119574186968814
>10k+	0.001995374440275735	5.033658747882351
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG	10545	2.4485161458103555	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC	10432	2.4222778978751665	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC	8833	2.05099507974802	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4170	0.9682610078737963	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAAGCCAT	3501	0.812921292222101	No Hit
GCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	2716	0.6306467379820698	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT	2109	0.4897032291620711	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTA	1912	0.4439604429387766	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCACCCATCGAATGC	1818	0.4221339358068493	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT	1569	0.36431691159568025	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTC	1353	0.3141623845691238	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGAAGCCA	646	0.14999918731090467	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGAACCCATCGAATGC	615	0.1428010838950563	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATG	578	0.1342097991729147	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	577	0.1339776022885325	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCATCGAATGC	575	0.13351320851976808	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGT	479	0.11122230761907637	No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT	451	0.1047207948563746	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT	447	0.1037920073188458	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10727496058457887	0.0
2	0.0	0.0	0.0	0.9190352683847688	0.0
3	0.0	0.0	0.0	1.0270068196224944	0.0
4	0.0	0.0	0.0	1.5285520898880578	0.0
5	0.0	0.0	0.0	4.012594359008891	0.0
6	0.0	0.0	0.0	4.381323011407833	0.0
7	0.0	0.0	0.0	5.114136378518073	0.0
8	0.0	0.0	0.0	5.749891447956552	0.0
9	0.0	0.0	0.0	5.893853516273519	0.0
10	0.0	0.0	0.0	8.731996034077214	0.0
11	0.0	0.0	0.0	9.25374243328403	0.0
12	0.0	0.0	0.0	12.183602720418698	0.0
13	0.0	0.0	0.0	12.509839342975695	0.0
14	0.0	0.0	0.0	12.682129431187292	0.0
15	0.0	0.0	0.0	13.299773143643959	0.0
16	0.0	0.0	0.0	13.674074521268073	0.0
17	0.0	0.0	0.0	14.01888689457565	0.0
18	0.0	0.0	0.0	14.370432977530308	0.0
19	0.0	0.0	0.0	15.462919318548584	0.0
20	0.0	0.0	0.0	15.840935846322814	0.0
21	0.0	0.0	0.0	16.076847880855134	0.0
22	0.0	0.0	0.0	16.39844056572449	0.0
23	0.0	0.0	0.0	16.648516610204123	0.0
24	0.0	0.0	0.0	16.8693358472516	0.0
25	0.0	0.0	0.0	17.07668766500491	0.0
26	0.0	0.0	0.0	17.282414104567543	0.0
27	0.0	0.0	0.0	17.515307579602897	0.0
28	0.0	0.0	0.0	17.695027968114722	0.0
29	0.0	0.0	0.0	17.88612600396128	0.0
30	0.0	0.0	0.0	18.094638806136498	0.0
31	0.0	0.0	0.0	18.307795545999365	0.0
32	0.0	0.0	0.0	18.533955311387633	0.0
33	0.0	0.0	0.0	18.758257501700843	0.0
34	0.0	0.0	0.0	18.95817901915392	0.0
35	0.0	0.0	0.0	19.18201681569837	0.0
36	2.3219688438220535E-4	0.0	0.0	19.41189173123675	0.0
37	2.3219688438220535E-4	0.0	0.0	19.64548179692525	0.0
38	2.3219688438220535E-4	0.0	0.0	19.863282474475756	0.0
39	2.3219688438220535E-4	0.0	0.0	20.079689970719972	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGA	20	7.028367E-4	45.000004	12
GATACCC	40	6.7975634E-9	45.000004	35
AGTCATA	25	3.8867285E-5	45.000004	35
ATAGGTA	20	7.028367E-4	45.000004	16
CGAAAGG	30	2.1623273E-6	45.000004	2
GCGCGAC	20	7.028367E-4	45.000004	9
CACGCTA	20	7.028367E-4	45.000004	41
GGCCCGT	20	7.028367E-4	45.000004	38
ATCCGGC	40	6.7975634E-9	45.000004	6
CACTATA	25	3.8867285E-5	45.000004	16
TCGTCAC	40	6.7975634E-9	45.000004	12
GTCAAGT	20	7.028367E-4	45.000004	40
CGGGATA	25	3.8867285E-5	45.000004	6
ACGCTAA	20	7.028367E-4	45.000004	42
CGTACAC	30	2.1623273E-6	45.000004	13
CGTAAGG	60	0.0	45.000004	2
ACGTAAG	20	7.028367E-4	45.000004	1
CCAACGG	30	2.1623273E-6	45.000004	2
GGTTCGA	20	7.028367E-4	45.000004	38
CCACGCA	30	2.1623273E-6	45.000004	41
>>END_MODULE