FastQCFastQC Report
Sat 18 Jun 2016
SRR3551892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509399
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG110032.159996387900251No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC99391.951122793723584No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC98241.9285471702928354No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35460.6961144407429146No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAAAGGT28390.5573234340860505No Hit
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC26420.5186504096003329Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT20480.40204240683629144No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTA19230.37750368571591225No Hit
GAACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCT17190.33745649284745355No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTC15040.2952498925204015No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7170.1407541043464946No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATG6240.12249729583293253No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTT5680.11150394877100268No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCAAAGG5380.10561465570211169No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCGTCAAAGGT5130.10070691147803588No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC207.029582E-445.00000425
TATTTCG207.029582E-445.00000444
AACCGGT207.029582E-445.00000423
GATCGAC207.029582E-445.0000049
CGCGGTA207.029582E-445.00000430
AAGCGCG207.029582E-445.0000041
TACGCGG253.8877355E-545.0000042
TATAGCG253.8877355E-545.0000041
ACGAGCC207.029582E-445.00000440
TAAGACG253.8877355E-545.0000041
TCGAATA502.1827873E-1145.00000444
ATCGACT207.029582E-445.00000410
TTTAGCG351.2101555E-745.01
CGCGCGA351.2101555E-745.041
CGAATGC2650.045.045
CGATCGA302.163113E-644.99999641
TCGAATG2750.044.1818244
GATACCT11650.043.2618035
CGGCGAT2200.042.95454431
TGATACC11900.042.352944