##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551892_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	509399
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.442258426106058	28.0	25.0	31.0	16.0	33.0
2	27.627184191566926	30.0	25.0	33.0	16.0	33.0
3	27.584482890622088	30.0	25.0	33.0	16.0	34.0
4	31.920881273814828	35.0	30.0	35.0	19.0	37.0
5	33.27744655957314	35.0	32.0	35.0	28.0	37.0
6	33.009120551866026	35.0	32.0	35.0	28.0	37.0
7	33.912789385138176	35.0	33.0	36.0	31.0	37.0
8	33.73087304843551	35.0	33.0	37.0	28.0	37.0
9	35.87365110650001	39.0	35.0	39.0	30.0	39.0
10	35.12296647618076	37.0	34.0	39.0	30.0	39.0
11	35.771846823413476	37.0	35.0	39.0	31.0	39.0
12	35.72624406408336	37.0	35.0	39.0	31.0	39.0
13	35.73464612219498	37.0	35.0	39.0	30.0	39.0
14	36.67946540923716	38.0	36.0	40.0	31.0	41.0
15	36.83666438292969	39.0	35.0	40.0	31.0	41.0
16	36.4654602776998	38.0	34.0	40.0	31.0	41.0
17	36.32437048364838	38.0	35.0	40.0	30.0	41.0
18	36.3245746458081	38.0	35.0	40.0	30.0	41.0
19	35.8796738902118	37.0	34.0	40.0	30.0	41.0
20	35.809962328155336	38.0	34.0	40.0	29.0	41.0
21	36.3113237364031	38.0	35.0	40.0	30.0	41.0
22	36.59006986664677	39.0	35.0	40.0	31.0	41.0
23	36.85600678446561	39.0	35.0	40.0	31.0	41.0
24	36.95928339081938	39.0	35.0	40.0	31.0	41.0
25	36.1618613307054	38.0	35.0	40.0	30.0	41.0
26	36.25799226146891	38.0	35.0	40.0	30.0	41.0
27	36.31499865527808	38.0	35.0	40.0	30.0	41.0
28	36.296253035439804	39.0	35.0	40.0	30.0	41.0
29	36.429368726675946	39.0	35.0	40.0	30.0	41.0
30	35.96224570523303	38.0	35.0	40.0	29.0	41.0
31	36.283174878631485	38.0	35.0	40.0	30.0	41.0
32	35.876028417802154	38.0	35.0	40.0	28.0	41.0
33	35.84121484337425	39.0	35.0	40.0	27.0	41.0
34	35.71291070457539	39.0	35.0	40.0	27.0	41.0
35	35.583305032008305	39.0	35.0	40.0	26.0	41.0
36	35.45606292905954	39.0	35.0	40.0	25.0	41.0
37	35.23417203410293	39.0	35.0	40.0	24.0	41.0
38	35.38382486027652	39.0	35.0	40.0	24.0	41.0
39	35.33716791748708	38.0	35.0	40.0	25.0	41.0
40	35.069701746568015	38.0	35.0	40.0	23.0	41.0
41	34.975647773160134	38.0	34.0	40.0	23.0	41.0
42	34.95101089715528	38.0	34.0	40.0	23.0	41.0
43	34.93223975704703	38.0	35.0	40.0	23.0	41.0
44	35.15019267803824	38.0	35.0	40.0	24.0	41.0
45	35.162903735578595	38.0	35.0	40.0	24.0	41.0
46	35.03907545951209	38.0	35.0	40.0	24.0	41.0
47	34.86364519757597	38.0	34.0	40.0	24.0	41.0
48	35.01178840162623	38.0	34.0	40.0	24.0	41.0
49	35.004440526973944	38.0	34.0	40.0	25.0	41.0
50	34.774196651347964	38.0	34.0	40.0	24.0	41.0
51	33.927834565831496	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	23.0
10	32.0
11	36.0
12	48.0
13	50.0
14	56.0
15	80.0
16	106.0
17	194.0
18	574.0
19	1320.0
20	2506.0
21	3731.0
22	4665.0
23	5256.0
24	6317.0
25	7373.0
26	7629.0
27	7536.0
28	7978.0
29	9383.0
30	11465.0
31	15426.0
32	20794.0
33	30638.0
34	41039.0
35	42654.0
36	58810.0
37	88495.0
38	103329.0
39	31850.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.03896748913916	16.536742317907965	18.044793963081986	15.379496229870886
2	30.621968241005575	32.50752357189551	20.36242709545955	16.50808109163936
3	29.492598140161245	30.90877681346057	24.634716597402036	14.963908448976145
4	26.051287890239283	21.207933270383332	34.944120424264675	17.796658415112713
5	32.29060127719136	24.830830056596106	23.4464535658688	19.43211510034374
6	22.593291309955458	39.64063533693627	24.775078082210605	12.990995270897665
7	67.84524508293106	5.655291824287052	21.809819022023994	4.689644070757893
8	66.71018199878681	15.566186820154732	11.994330573872347	5.729300607186116
9	61.938087825064436	7.426987489178424	12.26307864758274	18.371846038174397
10	42.72289501942485	22.449592559074517	20.024774292843134	14.802738128657497
11	35.88405159805967	21.584651717023394	24.691842740170276	17.83945394474665
12	27.58014837092338	19.662190149568413	27.7536862066867	25.0039752728215
13	28.049132408976067	22.197138196188057	31.911134493785813	17.84259490105006
14	19.985119719512603	32.09429150822832	26.46216423667891	21.458424535580164
15	17.779186845675003	23.313551852280824	35.89131505951131	23.015946242532866
16	19.787828401704754	23.98198661559995	28.4382183710608	27.791966611634493
17	19.613112707327655	28.0473656210554	30.30944308881643	22.030078582800517
18	19.703807820588576	24.3035420171614	30.017334152599435	25.97531600965059
19	21.689088514111727	25.409943875037055	26.773118910716352	26.127848700134866
20	28.082308759930818	24.09702414021229	30.93351184434991	16.88715525550698
21	23.38441967887648	31.900141146723886	26.213439759402746	18.501999414996888
22	21.051867004057723	22.11390285414773	32.656326376769485	24.177903765025057
23	25.216382442839503	27.40700315469799	28.117251898806238	19.25936250365627
24	23.520266038998898	26.01928939789831	27.592712196137015	22.867732366965775
25	19.992972110271122	32.90249882704913	26.762518183192352	20.342010879487397
26	20.39069570219023	24.25956862891368	32.582906523177314	22.766829145718777
27	25.966482070047253	26.51673835245063	26.956472234927826	20.560307342574287
28	18.51318907182778	29.13629590949334	31.154556644202287	21.19595837447659
29	24.68320511033591	23.500438752333633	31.19362228822593	20.62273384910453
30	19.689084587916348	30.358520531057188	30.620594072622836	19.331800808403628
31	23.34751344231143	23.782535890333513	24.72501909112503	28.144931576230025
32	28.31650631430372	28.985530006929732	24.63294980948137	18.065013869285178
33	25.45705821958818	27.002016101327253	23.776450287495656	23.764475391588913
34	24.648065661691522	26.740138869530565	24.99219668668372	23.619598782094194
35	25.845555252366026	24.650225069150117	27.335350089026477	22.16886958945738
36	25.728750939833017	29.240340087043755	23.342605698087354	21.688303275035874
37	21.180057283190585	27.723258192497436	29.82612843762944	21.27055608668254
38	20.98237334584481	28.333781573972466	23.22148257063716	27.462362509545564
39	23.118223632162607	30.966688195304666	24.914261708405395	21.000826464127336
40	23.946061927879718	26.149050155182874	27.389924204798206	22.514963712139206
41	22.244056230970223	27.29922909153728	25.290980155045457	25.165734522447043
42	24.23149633195197	24.736405057724888	28.528324555014834	22.503774055308316
43	26.786075355467915	24.609588946974768	25.037936862852106	23.56639883470521
44	22.592506070879605	24.406800955635955	29.345562123207934	23.6551308502765
45	22.034397397717704	23.610175913183966	27.225612928176147	27.129813760922183
46	27.111556952408623	28.166133031278033	24.481987597148798	20.240322419164546
47	19.83356857787314	28.21579940282568	31.925072487382188	20.025559531918987
48	22.458622808446815	26.437232896020607	27.246814383224155	23.857329912308426
49	22.076603998044757	24.34908588356082	30.946271979332508	22.628038139061914
50	22.55736662223522	25.02929923301773	28.609989418903453	23.80334472584359
51	21.512017102507073	24.142764316380678	26.87127379519787	27.473944785914384
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	257.0
1	720.0
2	1183.0
3	3727.5
4	6272.0
5	4327.5
6	2383.0
7	2362.5
8	2342.0
9	2275.5
10	2209.0
11	2187.0
12	2165.0
13	2019.5
14	1874.0
15	1769.0
16	1664.0
17	1648.5
18	1633.0
19	1557.0
20	1481.0
21	1696.5
22	1912.0
23	1992.5
24	2073.0
25	2380.5
26	3227.5
27	3767.0
28	4382.0
29	4997.0
30	5831.0
31	6665.0
32	7722.0
33	8779.0
34	10332.0
35	11885.0
36	12925.5
37	13966.0
38	14414.5
39	14863.0
40	16462.0
41	18061.0
42	20739.5
43	23418.0
44	26150.5
45	28883.0
46	50233.5
47	71584.0
48	55486.0
49	39388.0
50	39223.5
51	39059.0
52	36458.5
53	33858.0
54	31891.5
55	29925.0
56	29000.5
57	28076.0
58	26821.5
59	25567.0
60	24187.0
61	22807.0
62	20356.0
63	17905.0
64	15140.0
65	12375.0
66	10131.0
67	7887.0
68	6865.0
69	5843.0
70	4866.0
71	3889.0
72	3157.5
73	2426.0
74	1958.0
75	1191.5
76	893.0
77	696.5
78	500.0
79	412.5
80	325.0
81	218.5
82	112.0
83	84.0
84	56.0
85	30.0
86	4.0
87	4.0
88	4.0
89	4.0
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	509399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.998986864629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.86940170223235	20.483969799858322
2	8.269094167365605	4.465143296141686
3	2.905021637131382	2.3529792306711963
4	1.5677761746543648	1.6931347338468596
5	1.0653496004420795	1.438167993428673
6	0.8084417397909694	1.309626474806052
7	0.6506044241968016	1.2295962212071272
8	0.6021964821111929	1.3006955928356712
9	0.5247593896516907	1.2751174681467055
>10	7.19365366319966	44.16742916297795
>50	0.48458721713380265	8.060455584893585
>100	0.0481668017030587	2.3821968324377494
>500	0.0036490001290195983	0.5832342947903836
>1k	0.005108600180627437	3.196163343173923
>5k	0.0014596000516078392	3.8940741107913346
>10k+	7.298000258039196E-4	2.1680158599927672
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG	11003	2.159996387900251	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC	9939	1.951122793723584	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	9824	1.9285471702928354	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3546	0.6961144407429146	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAAAGGT	2839	0.5573234340860505	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	2642	0.5186504096003329	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT	2048	0.40204240683629144	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTA	1923	0.37750368571591225	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCT	1719	0.33745649284745355	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTC	1504	0.2952498925204015	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	717	0.1407541043464946	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATG	624	0.12249729583293253	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTT	568	0.11150394877100268	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCAAAGG	538	0.10561465570211169	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCGTCAAAGGT	513	0.10070691147803588	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06753056052328332	0.0
2	0.0	0.0	0.0	0.7707121529488672	0.0
3	0.0	0.0	0.0	0.9018470786161732	0.0
4	0.0	0.0	0.0	1.3873211372617535	0.0
5	0.0	0.0	0.0	3.793097355903722	0.0
6	0.0	0.0	0.0	4.168637943930004	0.0
7	0.0	0.0	0.0	4.826471979725127	0.0
8	0.0	0.0	0.0	5.451325974334461	0.0
9	0.0	0.0	0.0	5.613477843497926	0.0
10	0.0	0.0	0.0	8.169038415858688	0.0
11	0.0	0.0	0.0	8.698289552983026	0.0
12	0.0	0.0	0.0	11.178074554524057	0.0
13	0.0	0.0	0.0	11.496488999782096	0.0
14	0.0	0.0	0.0	11.650395858649114	0.0
15	0.0	0.0	0.0	12.194762847983604	0.0
16	0.0	0.0	0.0	12.570499745778848	0.0
17	0.0	0.0	0.0	12.923464710374382	0.0
18	0.0	0.0	0.0	13.296453271404145	0.0
19	0.0	0.0	0.0	14.140585277945187	0.0
20	0.0	0.0	0.0	14.45605507666878	0.0
21	0.0	0.0	0.0	14.714398732624131	0.0
22	0.0	0.0	0.0	15.056174040388772	0.0
23	0.0	0.0	0.0	15.32021067964405	0.0
24	0.0	0.0	0.0	15.541648099034353	0.0
25	0.0	0.0	0.0	15.763281828193616	0.0
26	0.0	0.0	0.0	15.961751004615243	0.0
27	0.0	0.0	0.0	16.257589826442533	0.0
28	0.0	0.0	0.0	16.460770437319272	0.0
29	0.0	0.0	0.0	16.655902347668526	0.0
30	0.0	0.0	0.0	16.872824642372677	0.0
31	0.0	0.0	0.0	17.084250263545865	0.0
32	0.0	0.0	0.0	17.300976248481053	0.0
33	0.0	0.0	0.0	17.526143553481653	0.0
34	0.0	0.0	0.0	17.770549215840628	0.0
35	0.0	0.0	0.0	18.00003533575841	0.0
36	0.0	0.0	0.0	18.222846923531456	0.0
37	0.0	0.0	0.0	18.43937659869768	0.0
38	0.0	0.0	0.0	18.668077479539615	0.0
39	0.0	0.0	0.0	18.906201229291774	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.029582E-4	45.000004	25
TATTTCG	20	7.029582E-4	45.000004	44
AACCGGT	20	7.029582E-4	45.000004	23
GATCGAC	20	7.029582E-4	45.000004	9
CGCGGTA	20	7.029582E-4	45.000004	30
AAGCGCG	20	7.029582E-4	45.000004	1
TACGCGG	25	3.8877355E-5	45.000004	2
TATAGCG	25	3.8877355E-5	45.000004	1
ACGAGCC	20	7.029582E-4	45.000004	40
TAAGACG	25	3.8877355E-5	45.000004	1
TCGAATA	50	2.1827873E-11	45.000004	44
ATCGACT	20	7.029582E-4	45.000004	10
TTTAGCG	35	1.2101555E-7	45.0	1
CGCGCGA	35	1.2101555E-7	45.0	41
CGAATGC	265	0.0	45.0	45
CGATCGA	30	2.163113E-6	44.999996	41
TCGAATG	275	0.0	44.18182	44
GATACCT	1165	0.0	43.261803	5
CGGCGAT	220	0.0	42.954544	31
TGATACC	1190	0.0	42.35294	4
>>END_MODULE