##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551891_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 365777 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.630280198044165 30.0 25.0 31.0 16.0 33.0 2 27.653231340406858 30.0 25.0 33.0 16.0 33.0 3 27.608854575328685 30.0 25.0 33.0 16.0 34.0 4 31.989518203714287 35.0 32.0 35.0 19.0 37.0 5 33.275960489587916 35.0 32.0 35.0 28.0 37.0 6 33.175407420368145 35.0 32.0 35.0 28.0 37.0 7 33.941584626698784 35.0 33.0 36.0 31.0 37.0 8 33.454449022218455 35.0 33.0 37.0 28.0 37.0 9 35.6473370386875 38.0 35.0 39.0 28.0 39.0 10 35.02733086005954 37.0 34.0 39.0 30.0 39.0 11 35.698009989693176 37.0 35.0 39.0 31.0 39.0 12 35.66387170325089 37.0 35.0 39.0 31.0 39.0 13 35.497327606711195 37.0 34.0 39.0 30.0 39.0 14 36.42794106791843 38.0 35.0 40.0 30.0 41.0 15 36.572449880665 38.0 35.0 40.0 31.0 41.0 16 36.347963376592844 38.0 34.0 40.0 31.0 41.0 17 36.116114463183855 38.0 34.0 40.0 30.0 41.0 18 36.141178915021996 38.0 34.0 40.0 30.0 41.0 19 35.81482706676472 37.0 34.0 40.0 30.0 41.0 20 35.51110649384734 37.0 34.0 40.0 27.0 41.0 21 36.26100055498296 38.0 35.0 40.0 30.0 41.0 22 36.46687735970277 38.0 35.0 40.0 31.0 41.0 23 36.68796561839591 38.0 35.0 40.0 31.0 41.0 24 36.7155124570435 39.0 35.0 40.0 31.0 41.0 25 36.17792534795791 38.0 35.0 40.0 30.0 41.0 26 36.247380234405114 38.0 35.0 40.0 30.0 41.0 27 36.20206847341413 38.0 35.0 40.0 30.0 41.0 28 36.16269749054752 38.0 35.0 40.0 30.0 41.0 29 36.218488313918044 38.0 35.0 40.0 30.0 41.0 30 35.770182378881124 38.0 35.0 40.0 29.0 41.0 31 36.0975922488292 38.0 35.0 40.0 30.0 41.0 32 35.787348575771574 38.0 35.0 40.0 29.0 41.0 33 35.56217312734262 38.0 35.0 40.0 26.0 41.0 34 35.6810187627981 38.0 35.0 40.0 27.0 41.0 35 35.33006996065909 38.0 35.0 40.0 25.0 41.0 36 35.52510956129007 38.0 35.0 40.0 27.0 41.0 37 35.23734406482638 38.0 35.0 40.0 24.0 41.0 38 35.06110553698018 38.0 35.0 40.0 24.0 41.0 39 35.16197847322275 38.0 35.0 40.0 24.0 41.0 40 34.80886168348474 38.0 34.0 40.0 23.0 41.0 41 34.947812464971825 38.0 34.0 40.0 23.0 41.0 42 35.02085150241814 38.0 35.0 40.0 24.0 41.0 43 35.114367497136236 38.0 35.0 40.0 24.0 41.0 44 35.43104405143024 38.0 35.0 40.0 26.0 41.0 45 35.42126486903222 38.0 35.0 40.0 26.0 41.0 46 35.181955125664 38.0 35.0 40.0 26.0 41.0 47 34.84850332306296 37.0 34.0 40.0 25.0 41.0 48 35.01735210251054 38.0 34.0 40.0 26.0 41.0 49 35.13055768952121 38.0 35.0 40.0 26.0 41.0 50 35.00723938355883 38.0 34.0 40.0 26.0 41.0 51 34.042941464334824 36.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 12.0 10 20.0 11 21.0 12 27.0 13 22.0 14 31.0 15 42.0 16 68.0 17 122.0 18 346.0 19 833.0 20 1700.0 21 2470.0 22 2898.0 23 3587.0 24 4374.0 25 5207.0 26 5392.0 27 5523.0 28 5704.0 29 6760.0 30 8651.0 31 11622.0 32 16290.0 33 24761.0 34 32496.0 35 32124.0 36 42163.0 37 62352.0 38 70280.0 39 19877.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.560776101285754 15.868411627849754 16.44881990939835 14.121992361466141 2 25.672745962704052 38.682858681655766 19.701348089136278 15.943047266503907 3 26.13176880995798 36.91046730658297 22.601749153172563 14.356014730286487 4 24.51028905590018 18.585914368590696 40.806010219341296 16.097786356167827 5 36.62504750161984 22.494853421620277 21.87042925060898 19.00966982615091 6 20.958671540310082 41.98979159433207 23.81232280870585 13.239214056652004 7 62.63789139284318 5.392356545108085 26.741703278226897 5.228048783821837 8 59.671876580539504 20.610645283875147 13.079007154632466 6.638470980952875 9 56.61099522386591 7.024771923877117 12.171350303600281 24.192882548656694 10 44.15750580271586 19.3027445684119 21.021551382399657 15.518198246472577 11 36.723741514638704 19.815625367368643 25.05679690084396 18.4038362171487 12 27.403581963874164 17.394478056302066 29.161210245586794 26.04072973423698 13 26.904917477042023 21.75505840990549 35.31331931750766 16.026704795544827 14 18.44320446610913 33.66368032981844 25.835686770901393 22.05742843317103 15 15.93293181364602 22.320976988711703 39.80102630837915 21.94506488926313 16 17.38190208788415 22.812259928863764 28.186572693198315 31.61926529005378 17 17.217867717215682 27.437755791096762 32.880416209876515 22.463960281811048 18 18.009060165073254 23.55232833119633 30.860606325712116 27.5780051780183 19 18.867780095522683 27.01235998982987 26.548142720838108 27.57171719380934 20 25.531403013311387 24.454243979255118 34.230145689860215 15.784207317573276 21 22.377022065356762 33.263709856005164 26.45245600461483 17.906812074023243 22 18.755143160997 20.66012898569347 33.93050957277248 26.65421828053705 23 22.428419501499548 28.940857407655486 29.777432698064665 18.853290392780302 24 22.448103625979762 26.290335368270835 26.256708322283796 25.00485268346561 25 16.927800271750275 36.21277445000642 25.471803858635177 21.38762141960812 26 19.0566930124092 22.854635474619784 33.62513225271135 24.46353926025967 27 25.798779037500992 25.226299083868042 26.610202391074345 22.364719487556624 28 17.6528321901049 28.474179623103694 33.31319355782348 20.55979462896792 29 26.951667272682535 21.303690499949425 30.792532061884696 20.95211016548334 30 18.43199545078012 32.43232898733381 31.33247853200174 17.80319702988433 31 28.055345196663538 21.486042042009203 22.195490695150326 28.263122066176933 32 32.25599203886521 28.789125614787153 22.796403273032475 16.158479073315164 33 24.04115075578836 26.57848907941177 22.563747857301035 26.816612307498833 34 27.47165622770158 23.755730950825228 23.90746274369356 24.86515007777963 35 22.338747378867453 26.804856510934254 27.728370017797726 23.12802609240056 36 27.84893528023905 30.379165447800162 22.4262323765573 19.345666895403482 37 23.913203946666957 23.97717735122766 31.797789363464624 20.31182933864076 38 23.501751066906888 30.33979719883973 21.96939665424562 24.189055080007765 39 27.245562186796874 24.88948184276212 23.996314694472314 23.86864127596869 40 19.357969473203617 30.47129808599228 27.635417207752266 22.53531523305183 41 19.13734324465453 29.712639121650625 23.458555349297523 27.69146228439732 42 20.81459468473961 26.55607104875375 30.102220751988234 22.52711351451841 43 24.40858774608573 27.642798754432345 23.840755432955053 24.107858066526873 44 22.963444940496533 22.31687612944499 30.490708819854724 24.228970110203758 45 21.77939017488798 22.467240969224417 27.085355284777336 28.66801357111027 46 29.85288850857216 27.75625586081137 23.560803440347534 18.830052190268933 47 18.854930736486985 26.824814026032257 35.091052745251886 19.229202492228872 48 23.73221935769608 25.18091624131643 24.838357797237116 26.24850660375037 49 20.602170174723945 21.914171749453903 34.53306249436132 22.950595581460835 50 21.898861874858177 24.024473928103735 28.578341448478174 25.498322748559914 51 22.503875312007043 21.400197388026037 26.33571821082244 29.760209089144478 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 230.0 1 430.5 2 631.0 3 2447.0 4 4263.0 5 2943.5 6 1624.0 7 1621.0 8 1618.0 9 1560.0 10 1502.0 11 1451.5 12 1401.0 13 1364.5 14 1328.0 15 1227.5 16 1127.0 17 1009.5 18 892.0 19 895.0 20 898.0 21 891.0 22 884.0 23 936.0 24 988.0 25 1241.5 26 1951.0 27 2407.0 28 3179.0 29 3951.0 30 4000.5 31 4050.0 32 5055.5 33 6061.0 34 6518.5 35 6976.0 36 7647.5 37 8319.0 38 8953.0 39 9587.0 40 10879.0 41 12171.0 42 14257.0 43 16343.0 44 18925.0 45 21507.0 46 47683.5 47 73860.0 48 52713.5 49 31567.0 50 30846.0 51 30125.0 52 26644.5 53 23164.0 54 21406.0 55 19648.0 56 18306.5 57 16965.0 58 16205.5 59 15446.0 60 14483.5 61 13521.0 62 12159.5 63 10798.0 64 8982.0 65 7166.0 66 5874.0 67 4582.0 68 3933.5 69 3285.0 70 2753.5 71 2222.0 72 1726.0 73 1230.0 74 1107.5 75 707.0 76 429.0 77 345.5 78 262.0 79 212.0 80 162.0 81 107.0 82 52.0 83 41.0 84 30.0 85 22.5 86 15.0 87 11.0 88 7.0 89 4.5 90 2.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 365777.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.39477058426309 #Duplication Level Percentage of deduplicated Percentage of total 1 76.2196258778199 20.11799539063418 2 9.411057941292233 4.968054306312316 3 3.0959335446315746 2.4514936696402456 4 1.6085596503221264 1.6983025176541993 5 0.9560209641000146 1.261697701058295 6 0.6390736022207031 1.0120920670244438 7 0.5914279203695648 1.0927422992697737 8 0.4578128560478943 0.9667092244728346 9 0.40602407142709174 0.9645220995305882 >10 6.07378866032772 38.28206803598914 >50 0.4692063886644708 7.60135273677678 >100 0.05696766308288277 2.8159233631420237 >500 0.004143102769664201 0.6853902787764129 >1k 0.007250429846912353 5.312253094098317 >5k 0.0 0.0 >10k+ 0.003107327077248151 10.769403215620446 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG 15096 4.12710476601864 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC 12181 3.330171115187669 No Hit GAATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC 12115 3.3121273344141375 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGACAAT 3962 1.0831736276474464 No Hit GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC 3626 0.9913143800731047 No Hit GAATGACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCT 2781 0.7602993080483464 No Hit GAACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCT 2483 0.6788289039496743 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2472 0.6758216071540857 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGACAATCGTA 2090 0.5713863911618281 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTC 2017 0.5514288760638313 No Hit GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTGGACAA 732 0.20012193221553023 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATG 644 0.17606355785082167 No Hit CCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC 592 0.16184724572622117 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGGACAATCGT 539 0.14735754298383988 No Hit GAATGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTT 494 0.13505496518370483 No Hit GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTAT 417 0.11400388761458485 No Hit TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC 395 0.1079892940234077 No Hit CTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT 390 0.10662234093450382 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.11263693452568095 0.0 2 0.0 0.0 0.0 1.3825363541173994 0.0 3 0.0 0.0 0.0 1.5531320996126055 0.0 4 0.0 0.0 0.0 2.470357622267119 0.0 5 0.0 0.0 0.0 6.478264078933339 0.0 6 0.0 0.0 0.0 6.944668472867348 0.0 7 0.0 0.0 0.0 8.058188459088461 0.0 8 0.0 0.0 0.0 9.112382681251145 0.0 9 0.0 0.0 0.0 9.317152253968949 0.0 10 0.0 0.0 0.0 14.054738269492068 0.0 11 0.0 0.0 0.0 14.736574470237331 0.0 12 0.0 0.0 0.0 18.81118823764206 0.0 13 0.0 0.0 0.0 19.25025356979799 0.0 14 0.0 0.0 0.0 19.45967078301807 0.0 15 0.0 0.0 0.0 20.241294559253316 0.0 16 0.0 0.0 0.0 20.743513124116607 0.0 17 0.0 0.0 0.0 21.22905486129527 0.0 18 0.0 0.0 0.0 21.71705711403396 0.0 19 0.0 0.0 0.0 23.21578448070819 0.0 20 0.0 0.0 0.0 23.682462265259982 0.0 21 0.0 0.0 0.0 24.014085084628064 0.0 22 0.0 0.0 0.0 24.46764011952638 0.0 23 0.0 0.0 0.0 24.83070285993925 0.0 24 0.0 0.0 0.0 25.136626961235944 0.0 25 0.0 0.0 0.0 25.392520579478752 0.0 26 0.0 0.0 0.0 25.65060132266381 0.0 27 0.0 0.0 0.0 26.021592390992325 0.0 28 0.0 0.0 0.0 26.26873750946615 0.0 29 0.0 0.0 0.0 26.512601940526604 0.0 30 0.0 0.0 0.0 26.788999855102972 0.0 31 0.0 0.0 0.0 27.05528231682145 0.0 32 0.0 0.0 0.0 27.326212419042204 0.0 33 0.0 0.0 0.0 27.590307755818436 0.0 34 0.0 0.0 0.0 27.88392927931499 0.0 35 0.0 0.0 0.0 28.170442646749247 0.0 36 0.0 0.0 0.0 28.47308606063257 0.0 37 0.0 0.0 0.0 28.745109725324447 0.0 38 0.0 0.0 0.0 29.033536827083168 0.0 39 0.0 0.0 0.0 29.28696992976595 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGGTA 20 7.026974E-4 45.0 16 CGAACCC 45 3.8380676E-10 45.0 34 TCACCCG 25 3.885574E-5 45.0 37 CCGATCG 40 6.7939254E-9 45.0 40 GCGAACC 45 3.8380676E-10 45.0 33 CGGCCAA 25 3.885574E-5 45.0 37 CGGTTAT 20 7.026974E-4 45.0 31 TAGAACG 20 7.026974E-4 45.0 1 CCTACGC 20 7.026974E-4 45.0 28 CGATTGA 20 7.026974E-4 45.0 10 TAACGGG 20 7.026974E-4 45.0 3 TCGGATC 20 7.026974E-4 45.0 41 ACGGCTA 50 2.1827873E-11 45.0 30 CGGATCA 20 7.026974E-4 45.0 42 CACAGTC 20 7.026974E-4 45.0 18 GCCTATA 25 3.885574E-5 45.0 32 ACCGTAA 25 3.885574E-5 45.0 41 ACTCCGC 25 3.885574E-5 45.0 15 ATCGGTT 20 7.026974E-4 45.0 33 TATCGGT 20 7.026974E-4 45.0 32 >>END_MODULE