##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551889_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	491774
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.39921589998658	28.0	25.0	31.0	16.0	33.0
2	27.64586171696755	30.0	25.0	33.0	16.0	33.0
3	27.478571457620777	30.0	25.0	33.0	16.0	34.0
4	31.881703790765677	35.0	30.0	35.0	19.0	37.0
5	33.2436180033918	35.0	32.0	35.0	28.0	37.0
6	32.98940570261949	35.0	32.0	35.0	28.0	37.0
7	33.985157816395336	35.0	33.0	36.0	31.0	37.0
8	33.74995628073058	35.0	35.0	37.0	29.0	37.0
9	35.934524802043214	39.0	35.0	39.0	30.0	39.0
10	35.142101859797386	37.0	34.0	39.0	30.0	39.0
11	35.72958106772623	37.0	35.0	39.0	31.0	39.0
12	35.7406471265256	37.0	35.0	39.0	31.0	39.0
13	35.68169728371162	37.0	35.0	39.0	30.0	39.0
14	36.66178976521736	38.0	35.0	40.0	31.0	41.0
15	36.76116874824615	38.0	35.0	40.0	31.0	41.0
16	36.48348835033979	38.0	34.0	40.0	31.0	41.0
17	36.33474522849927	38.0	35.0	40.0	30.0	41.0
18	36.34417232305896	38.0	35.0	40.0	31.0	41.0
19	35.98605660323645	37.0	34.0	40.0	30.0	41.0
20	35.897786381549246	38.0	34.0	40.0	30.0	41.0
21	36.39562481953092	38.0	35.0	40.0	31.0	41.0
22	36.66290816513276	39.0	35.0	40.0	31.0	41.0
23	36.92512210893622	39.0	35.0	40.0	31.0	41.0
24	36.97154587269762	39.0	35.0	40.0	31.0	41.0
25	36.263200169183406	38.0	35.0	40.0	30.0	41.0
26	36.357902207111394	38.0	35.0	40.0	30.0	41.0
27	36.44922057693168	38.0	35.0	40.0	30.0	41.0
28	36.34637455416512	39.0	35.0	40.0	30.0	41.0
29	36.4351856747205	39.0	35.0	40.0	30.0	41.0
30	36.1302854563275	38.0	35.0	40.0	30.0	41.0
31	36.431313977558794	39.0	35.0	40.0	30.0	41.0
32	36.088866023823954	39.0	35.0	40.0	30.0	41.0
33	35.90648346598234	39.0	35.0	40.0	27.0	41.0
34	35.9583243522431	39.0	35.0	40.0	29.0	41.0
35	35.76163034239305	39.0	35.0	40.0	27.0	41.0
36	35.81021770162717	39.0	35.0	40.0	28.0	41.0
37	35.72500579534502	39.0	35.0	41.0	28.0	41.0
38	35.70191388727342	39.0	35.0	40.0	27.0	41.0
39	35.48721973914847	39.0	35.0	40.0	26.0	41.0
40	35.171513744118236	38.0	34.0	40.0	24.0	41.0
41	35.2026255963105	38.0	34.0	40.0	24.0	41.0
42	35.34048160333812	38.0	35.0	40.0	26.0	41.0
43	35.365419887997334	38.0	35.0	40.0	26.0	41.0
44	35.619101457173414	38.0	35.0	40.0	28.0	41.0
45	35.64040595883475	38.0	35.0	40.0	28.0	41.0
46	35.34149629707955	38.0	35.0	40.0	27.0	41.0
47	35.164713872632554	38.0	34.0	40.0	26.0	41.0
48	35.25054191559538	38.0	34.0	40.0	27.0	41.0
49	35.26248439323754	37.0	35.0	40.0	28.0	41.0
50	34.99679527587876	37.0	34.0	40.0	27.0	41.0
51	34.14978831739783	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	11.0
10	23.0
11	29.0
12	18.0
13	28.0
14	29.0
15	65.0
16	71.0
17	182.0
18	425.0
19	1103.0
20	2012.0
21	2651.0
22	3451.0
23	4508.0
24	5394.0
25	6165.0
26	6718.0
27	6744.0
28	7487.0
29	8798.0
30	11114.0
31	14724.0
32	20723.0
33	31687.0
34	42538.0
35	42631.0
36	57983.0
37	86470.0
38	98272.0
39	29716.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.2735524854913	15.98945857243368	17.93649115243994	14.800497789635076
2	29.24473436985282	33.637605892137444	20.813422425748414	16.304237312261325
3	28.383973125866763	32.66744480188054	23.22814951583451	15.720432556418192
4	26.09288006279307	21.024901682480163	35.87216892312322	17.01004933160354
5	33.220747741848896	24.860200010573962	22.364134744821808	19.55491750275533
6	21.7726028622904	40.55358762358319	23.8483531052882	13.825456408838205
7	68.21263425882621	5.444574133646756	21.522284626678108	4.8205069808489265
8	66.48155453521333	16.278615786926515	11.415609609292073	5.824220068568082
9	61.687279116016704	7.374525696763147	11.725508058579754	19.21268712864039
10	45.18315323705605	20.385176930866617	19.226107927625293	15.205561904452045
11	35.638728358961636	21.897253616498634	24.496008329029188	17.96800969551054
12	28.25118855409192	19.526652486711377	27.90102770784954	24.321131251347165
13	27.26699662853262	22.64251465103889	32.01755277830873	18.072935942119756
14	20.1385189131593	31.287135960827534	26.771647138726323	21.80269798728684
15	17.984480676082917	23.229979624786996	36.440926116468134	22.344613582661953
16	20.57652498912102	23.473384115467674	28.25057851777443	27.699512377636882
17	19.87457653312294	27.98114581088061	30.056082672121747	22.088194983874708
18	20.396157584581534	24.578973268208568	29.43201551932392	25.59285362788598
19	21.75796199067051	26.762496593963892	25.916376221597726	25.563165193767865
20	27.209653214688046	25.346398955617826	31.534404014852353	15.909543814841776
21	23.59295123369678	31.6747123678763	26.68237849093283	18.049957907494093
22	20.606416768678297	22.484108553929243	32.01185910601211	24.89761557138035
23	24.773371508050445	27.389410582910035	28.289010805776638	19.54820710326288
24	23.67754293638948	26.158967330521744	26.813942990072675	23.3495467430161
25	19.83167064545909	32.943791253705974	26.08779642681394	21.136741674020993
26	20.016714995099374	24.506175600987447	31.675119058754632	23.80199034515855
27	25.026536579811054	27.671857397910422	25.353922736866934	21.947683285411593
28	18.399102026540646	29.121710379157907	30.77551883588803	21.703668758413418
29	25.84500197245076	23.426207973581363	29.53755180225063	21.19123825171725
30	20.344101152155257	32.66846152907637	27.258659465526847	19.72877785324153
31	24.35732673951856	23.26922529454587	23.659648537742946	28.713799428192626
32	30.82045817794353	28.79086734963622	22.944279282759965	17.44439518966029
33	24.660921480192123	26.621374859183284	23.652531447372166	25.065172213252428
34	25.20202369381057	24.414060117045636	27.81989287762265	22.564023311521144
35	22.613232907799112	29.764688657798093	24.891921899083723	22.730156535319068
36	23.20557817208718	29.493425841951794	22.837726272637433	24.463269713323598
37	23.828628597689182	28.76138226095727	27.24889888444692	20.16109025690663
38	20.73310097727818	29.183730738103275	22.24090740868773	27.842260875930812
39	25.881197460622158	25.852729099139037	26.89426443854291	21.3718090016959
40	20.646475820193828	29.549142492283043	26.679124963906187	23.125256723616946
41	18.946914639651546	25.668091440377083	27.649286054163092	27.73570786580828
42	20.792477845514405	27.07686864291321	29.247784551440297	22.882868960132093
43	22.589238145977625	24.8418989210491	24.00370902081037	28.565153912162906
44	22.83650620000244	24.779268525786236	27.925632505988524	24.4585927682228
45	21.092005677404664	24.050071780940023	26.629305331310725	28.228617210344588
46	26.860915786519822	27.70500270449434	23.88190510275045	21.552176406235386
47	18.655113934449563	27.32088316991138	32.81304013632278	21.21096275931627
48	21.930398923082553	25.384017861863377	27.905501307511173	24.780081907542893
49	21.72868024743073	23.529507456677255	32.183075965789165	22.558736330102853
50	21.587355167210955	24.048445017426705	28.527128315039025	25.837071500323322
51	20.875239439254617	22.793600312338594	27.880896509372192	28.450263739034597
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	255.0
1	631.5
2	1008.0
3	3062.0
4	5116.0
5	3580.5
6	2045.0
7	1977.5
8	1910.0
9	1850.5
10	1791.0
11	1692.5
12	1594.0
13	1582.5
14	1571.0
15	1478.5
16	1386.0
17	1355.5
18	1325.0
19	1315.0
20	1305.0
21	1363.5
22	1422.0
23	1547.0
24	1672.0
25	1947.5
26	2937.5
27	3652.0
28	4572.5
29	5493.0
30	6195.0
31	6897.0
32	7742.5
33	8588.0
34	9513.0
35	10438.0
36	11409.5
37	12381.0
38	12809.5
39	13238.0
40	15820.0
41	18402.0
42	19931.5
43	21461.0
44	24428.0
45	27395.0
46	53096.0
47	78797.0
48	59286.0
49	39775.0
50	39135.5
51	38496.0
52	34783.0
53	31070.0
54	29937.5
55	28805.0
56	27159.0
57	25513.0
58	24652.0
59	23791.0
60	23122.5
61	22454.0
62	19757.0
63	17060.0
64	15197.5
65	13335.0
66	10476.5
67	7618.0
68	6091.0
69	4564.0
70	3811.0
71	3058.0
72	2384.0
73	1710.0
74	1621.5
75	1201.5
76	870.0
77	632.5
78	395.0
79	315.5
80	236.0
81	160.5
82	85.0
83	51.0
84	17.0
85	17.0
86	17.0
87	10.5
88	4.0
89	2.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	491774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.93040453970015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.36286487384046	19.541895734494414
2	8.843288325982359	4.586200875078605
3	3.0460786571372207	2.3695815551794412
4	1.6175068726106097	1.6777043021015334
5	1.0632063339725124	1.378468517453939
6	0.7471240301306462	1.1623937005571268
7	0.6178249643184969	1.1214315881653103
8	0.5201895038132832	1.079097941694746
9	0.43282573796820256	1.010101183263877
>10	7.162026550160759	43.67212577649288
>50	0.521747361251207	8.311736376117084
>100	0.05351254987191866	2.4364659808219087
>500	0.003934746314111667	0.6340117087448636
>1k	0.005508644839756333	3.7026528661652875
>5k	0.0	0.0
>10k+	0.0023608477884669997	7.316131893669002
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG	12474	2.5365310081460186	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC	11981	2.436281706637602	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC	11398	2.317731315604322	No Hit
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	3235	0.657822495699244	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACGATACT	3172	0.6450117330318399	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2809	0.5711973386148922	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCT	2468	0.5018565438595778	No Hit
GAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	2395	0.4870123268005222	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACGATACTCGTA	2249	0.45732389268241097	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTC	1817	0.3694786629630684	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	676	0.137461516875638	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATG	666	0.13542806248398653	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGACGATAC	617	0.12546413596489445	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT	590	0.11997380910743552	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACGATACTCGT	558	0.11346675505415088	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07747461232192022	0.0
2	0.0	0.0	0.0	0.9128176764123358	0.0
3	0.0	0.0	0.0	1.0453989027480102	0.0
4	0.0	0.0	0.0	1.7164388519929887	0.0
5	0.0	0.0	0.0	4.581982780708212	0.0
6	0.0	0.0	0.0	5.007381439441695	0.0
7	0.0	0.0	0.0	5.803275488334072	0.0
8	0.0	0.0	0.0	6.541826123381878	0.0
9	0.0	0.0	0.0	6.715686473868078	0.0
10	0.0	0.0	0.0	9.722352137363911	0.0
11	0.0	0.0	0.0	10.29741303932294	0.0
12	0.0	0.0	0.0	13.294318121738847	0.0
13	0.0	0.0	0.0	13.638785295684603	0.0
14	0.0	0.0	0.0	13.819356045663252	0.0
15	0.0	0.0	0.0	14.45359047041934	0.0
16	0.0	0.0	0.0	14.867195093681245	0.0
17	0.0	0.0	0.0	15.258838409513313	0.0
18	0.0	0.0	0.0	15.641941216900445	0.0
19	0.0	0.0	0.0	16.60051161712494	0.0
20	0.0	0.0	0.0	16.96694009850053	0.0
21	0.0	0.0	0.0	17.238609605225165	0.0
22	0.0	0.0	0.0	17.61784884926816	0.0
23	0.0	0.0	0.0	17.911683008861793	0.0
24	0.0	0.0	0.0	18.18111571575561	0.0
25	0.0	0.0	0.0	18.419233225017997	0.0
26	0.0	0.0	0.0	18.655520625327895	0.0
27	0.0	0.0	0.0	18.954438420900658	0.0
28	0.0	0.0	0.0	19.19174254840638	0.0
29	0.0	0.0	0.0	19.421929585541324	0.0
30	0.0	0.0	0.0	19.671231093957793	0.0
31	2.0334543916514496E-4	0.0	0.0	19.932123292406676	0.0
32	2.0334543916514496E-4	0.0	0.0	20.19403221805138	0.0
33	2.0334543916514496E-4	0.0	0.0	20.446587253494492	0.0
34	2.0334543916514496E-4	0.0	0.0	20.70625937930838	0.0
35	2.0334543916514496E-4	0.0	0.0	20.972845250053886	0.0
36	2.0334543916514496E-4	0.0	0.0	21.23028057603696	0.0
37	2.0334543916514496E-4	0.0	0.0	21.50418688259241	0.0
38	2.0334543916514496E-4	0.0	0.0	21.78521027951864	0.0
39	2.0334543916514496E-4	0.0	0.0	22.054439640973293	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATGTAC	45	3.8380676E-10	45.000004	42
CGTTAGG	45	3.8380676E-10	45.000004	2
TCGGATA	35	1.2100281E-7	45.000004	17
CGAATAC	35	1.2100281E-7	45.000004	45
TTGATCG	20	7.029346E-4	45.0	1
TATCACG	25	3.8875394E-5	45.0	1
TCGCATC	20	7.029346E-4	45.0	44
GTCGATA	20	7.029346E-4	45.0	16
CCGATCG	20	7.029346E-4	45.0	40
CGACAGC	20	7.029346E-4	45.0	13
CCTCGTG	40	6.8012014E-9	45.0	15
TCGATAG	25	3.8875394E-5	45.0	17
ATCCGAT	25	3.8875394E-5	45.0	10
TGTTCGG	20	7.029346E-4	45.0	2
GTACTAT	20	7.029346E-4	45.0	34
CGTGTAC	40	6.8012014E-9	45.0	18
ACGTATG	20	7.029346E-4	45.0	1
GCGCTAG	20	7.029346E-4	45.0	1
CGATCGA	20	7.029346E-4	45.0	41
TGCGTAA	20	7.029346E-4	45.0	1
>>END_MODULE