SRR3551887_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551887_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	383305
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG	14586	3.805324741393929	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC	13950	3.6393994338711995	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC	11701	3.052660414030602	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	4234	1.1046033837283624	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTCTGT	3221	0.8403229803942031	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT	3208	0.8369314253662228	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT	2695	0.7030954461851529	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTA	2431	0.6342207902323215	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2406	0.627698569024667	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTC	2245	0.5856954644473722	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	783	0.20427596822373828	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATG	704	0.18366574920755013	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTTCTCTG	677	0.17662175030328328	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGT	538	0.14035820038872437	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT	526	0.13722753420905023	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT	525	0.13696664536074404	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTAT	486	0.12679198027680305	No Hit
GAATGATACGGCGACCACCGAGGAATCTGTCTCTTATACACATCTGACGCC	424	0.11061687168181997	Illumina Single End PCR Primer 1 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	393	0.1025293173843284	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACC	55	1.8189894E-12	45.000004	33
GGCAATA	30	2.1617016E-6	45.000004	8
ATCGAAT	30	2.1617016E-6	45.000004	43
CGGCGAT	150	0.0	45.0	31
TCTTGCG	20	7.027398E-4	45.0	1
AAATCGG	20	7.027398E-4	45.0	38
GCGACAG	20	7.027398E-4	45.0	1
ACGTTGT	20	7.027398E-4	45.0	37
CCGATCA	20	7.027398E-4	45.0	40
CGTGCGT	25	3.8859245E-5	45.0	11
CTATCGA	25	3.8859245E-5	45.0	41
GAGTCGT	25	3.8859245E-5	45.0	10
TCATTAC	20	7.027398E-4	45.0	43
GACCGGG	45	3.8380676E-10	45.0	3
CGCGCCA	20	7.027398E-4	45.0	41
ATGCTCG	25	3.8859245E-5	45.0	1
CGTCTCC	20	7.027398E-4	45.0	14
TTGTACG	20	7.027398E-4	45.0	36
CGATTCG	20	7.027398E-4	45.0	19
CGACAGG	35	1.2091004E-7	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content