FastQCFastQC Report
Sat 18 Jun 2016
SRR3551884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626339
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCG157132.5087053496588907No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTC142522.2754450864467963No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGC131912.106048002758889No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52020.8305406497120569No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGTCGATT39780.6351193203680435No Hit
GCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC37500.5987173080392567No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCT31340.500368011572008No Hit
GAACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCT28110.44879849410622685No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTA25260.4032959786952433No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTC21500.34326458994250714No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCGTCGAT8120.12964225443410038No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATG7810.12469285802097585No Hit
CCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC7710.12309627853287117No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTT7350.11734859237569431No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6510.10393732467561496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGCG302.1639135E-645.0000041
CTCGAGT302.1639135E-645.00000418
CGATACC302.1639135E-645.00000434
TAGTCGG302.1639135E-645.0000042
TTGGACG207.030825E-445.01
CGTTGAT207.030825E-445.025
ATAGCCG253.888765E-545.01
CATTACG253.888765E-545.035
CGTTACC207.030825E-445.041
TACGACG207.030825E-445.01
CTCTCGA207.030825E-445.011
AGAATCG253.888765E-545.028
TATCCGA253.888765E-545.012
GATGAAT22350.042.68456320
CGATGAA21750.042.51724219
CGATCGA850.042.3529441
CCGATGA21850.042.2196818
CGTTTTT24000.042.18751
TACGGCT14450.041.7301037
TGATACC18500.041.4729734
Produced by FastQC (version 0.11.5)