##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551884_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626339
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.52661577835645	30.0	25.0	31.0	16.0	33.0
2	27.649858942202226	30.0	25.0	33.0	16.0	33.0
3	27.576108145908208	30.0	25.0	33.0	16.0	34.0
4	31.891285709495975	35.0	30.0	35.0	19.0	37.0
5	33.29649279383848	35.0	32.0	35.0	28.0	37.0
6	33.07095518561035	35.0	32.0	35.0	28.0	37.0
7	33.942732290341176	35.0	33.0	36.0	31.0	37.0
8	33.63116299639652	35.0	33.0	37.0	28.0	37.0
9	35.81905326029514	39.0	35.0	39.0	30.0	39.0
10	35.159027299912665	37.0	34.0	39.0	30.0	39.0
11	35.831969588353914	37.0	35.0	39.0	31.0	39.0
12	35.83796155117277	37.0	35.0	39.0	31.0	39.0
13	35.816026145585695	37.0	35.0	39.0	31.0	39.0
14	36.79060860013507	38.0	36.0	40.0	31.0	41.0
15	36.863125240484784	39.0	35.0	40.0	31.0	41.0
16	36.56007529468866	38.0	34.0	40.0	31.0	41.0
17	36.38625728239819	38.0	35.0	40.0	30.0	41.0
18	36.37437234468874	38.0	35.0	40.0	30.0	41.0
19	35.97438767185182	37.0	34.0	40.0	30.0	41.0
20	35.844924234320395	38.0	34.0	40.0	30.0	41.0
21	36.41853692648869	38.0	35.0	40.0	31.0	41.0
22	36.669284524834	39.0	35.0	40.0	31.0	41.0
23	36.89641711597074	39.0	35.0	40.0	31.0	41.0
24	36.97480597567771	39.0	35.0	40.0	31.0	41.0
25	36.26117964872058	38.0	35.0	40.0	30.0	41.0
26	36.346992603047234	38.0	35.0	40.0	30.0	41.0
27	36.4185496991246	38.0	35.0	40.0	30.0	41.0
28	36.37050862232753	39.0	35.0	40.0	30.0	41.0
29	36.59286903737433	39.0	35.0	40.0	30.0	41.0
30	36.10141472908441	38.0	35.0	40.0	29.0	41.0
31	36.28627468511461	39.0	35.0	40.0	30.0	41.0
32	35.85681396176831	39.0	35.0	40.0	28.0	41.0
33	35.87240456046965	39.0	35.0	40.0	27.0	41.0
34	35.757465206541504	39.0	35.0	40.0	27.0	41.0
35	35.54844101995884	39.0	35.0	41.0	25.0	41.0
36	35.45661854043897	39.0	35.0	41.0	25.0	41.0
37	35.62443022069518	39.0	35.0	41.0	26.0	41.0
38	35.460525370446355	39.0	35.0	40.0	25.0	41.0
39	35.22692822896227	39.0	35.0	40.0	24.0	41.0
40	35.25859638310883	38.0	35.0	40.0	24.0	41.0
41	35.002865860181146	38.0	34.0	40.0	23.0	41.0
42	34.95400733468617	38.0	35.0	40.0	23.0	41.0
43	34.99591435308994	38.0	35.0	40.0	23.0	41.0
44	35.34296922273721	38.0	35.0	40.0	25.0	41.0
45	35.35779984960221	38.0	35.0	40.0	26.0	41.0
46	35.15032434512301	38.0	35.0	40.0	25.0	41.0
47	34.95657623108253	38.0	34.0	40.0	24.0	41.0
48	35.02759687645189	38.0	34.0	40.0	25.0	41.0
49	35.00326979479164	38.0	34.0	40.0	24.0	41.0
50	34.80390012437354	38.0	34.0	40.0	24.0	41.0
51	33.946193674671385	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	26.0
10	25.0
11	37.0
12	42.0
13	34.0
14	53.0
15	88.0
16	110.0
17	209.0
18	444.0
19	1263.0
20	2848.0
21	4644.0
22	5897.0
23	6740.0
24	7546.0
25	8560.0
26	9228.0
27	9288.0
28	9752.0
29	11494.0
30	14045.0
31	18275.0
32	24893.0
33	37249.0
34	49768.0
35	52473.0
36	72429.0
37	109224.0
38	130024.0
39	39617.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.09028657005232	15.736845382452632	17.859338153939	16.313529893556044
2	29.492974251962593	33.88835758271479	20.31040698407731	16.3082611812453
3	27.856001302808863	32.222646202775174	24.514360434205756	15.406992060210206
4	26.042287004321942	20.166714830147892	36.48407651447539	17.30692165105478
5	32.53988654706158	24.176524214522807	23.211232256014714	20.072356982400905
6	22.103206091270064	40.017945553446296	24.700202286621142	13.178646068662497
7	66.57752431191416	5.9930804244985545	22.448067260700675	4.981328002886616
8	64.79669955088218	16.712036133786974	12.39249032871975	6.098773986611084
9	60.40450937910621	7.455068261756013	12.914731479278792	19.225690879858988
10	42.74410502938505	21.983462629662213	20.563145517044283	14.709286823908457
11	35.04970950236214	22.57595327769786	24.52457854292963	17.84975867701037
12	26.876978760703068	19.495672471297492	29.115383203025836	24.5119655649736
13	27.691713273482893	21.872660013187748	32.53477749269964	17.900849220629723
14	19.35517347634428	31.915783625161453	26.190609238766864	22.5384336597274
15	17.96471240015391	22.564457905383506	36.31579703642915	23.155032658033427
16	19.454640378453202	23.916601073859365	28.75870734538325	27.870051202304186
17	19.44059047895788	27.666806633468454	31.037505248755064	21.855097638818595
18	19.540376696964422	24.90600138263784	29.511813889922227	26.04180803047551
19	21.59820799918255	26.10152010333062	26.273950688045932	26.026321209440894
20	26.768730671409575	24.683917175842478	31.68491823118152	16.862433921566435
21	23.689248154753255	30.97635625436066	27.82486800279082	17.509527588095263
22	20.341380626146545	22.897983360448578	32.41758855827276	24.343047455132126
23	24.751612146138115	26.88081055147452	28.76158118846184	19.605996113925524
24	22.64109372081253	27.152548380349938	26.804334393994306	23.402023504843225
25	19.654851446261528	33.83598977550496	25.89508237551869	20.614076402714822
26	19.383432933283736	24.79791295129315	32.92402357189956	22.894630543523554
27	25.173428446895375	26.0325478694445	26.626635096968254	22.16738858669187
28	18.599991378470765	28.99244658244178	31.71285837222335	20.69470366686411
29	22.33997882935599	22.573718066414514	33.51986703686023	21.566436067369267
30	19.99220869209805	29.769661477251137	27.373993955350056	22.864135875300757
31	26.42530642351825	22.832842917333902	24.399726026959843	26.342124632188003
32	26.99975572333832	27.141053008035588	26.840576748374286	19.018614520251813
33	24.87327150313169	23.027625614882673	24.509730353690255	27.58937252829538
34	24.5287296495987	23.926659524634424	28.155200298879677	23.389410526887197
35	23.697550368091402	25.1173885068629	25.71482855131167	25.47023257373403
36	23.80931093225873	27.88665562898047	27.969358446464295	20.334674992296502
37	20.85484058952101	25.416268187036096	30.77390997526898	22.95498124817391
38	23.188081853437197	24.94176476317138	24.58429061578474	27.285862767606677
39	24.844373414396994	24.605365465027727	29.085686824547093	21.46457429602819
40	20.291088372271247	25.73143297798796	27.873723335126826	26.10375531461397
41	22.375263236043104	24.843734782601754	27.966484603385705	24.814517377969437
42	20.757768556644248	26.76489888063812	29.79057666854531	22.686755894172325
43	22.090433455365226	24.215959727878992	28.74513641973436	24.94847039702142
44	22.178085669262174	22.88712661992946	29.786425561876236	25.14836214893213
45	21.953446935285843	22.631035270037472	27.902142450015088	27.513375344661593
46	25.89859485039252	26.32743610089744	26.588476847202553	21.18549220150749
47	18.903980113005897	26.064479459206595	33.3562176393295	21.675322788458008
48	23.7860008717324	24.769334178456077	26.981075743327494	24.46358920648403
49	20.926846324434532	23.105219378004563	33.441315326045476	22.526618971515425
50	21.81310759828144	23.385419078166937	30.212392969302567	24.589080354249056
51	21.260052463601976	22.410547642730215	28.74082565511648	27.588574238551328
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	308.0
1	863.5
2	1419.0
3	5034.0
4	8649.0
5	5876.5
6	3104.0
7	3019.5
8	2935.0
9	2826.0
10	2717.0
11	2650.5
12	2584.0
13	2449.5
14	2315.0
15	2099.0
16	1883.0
17	1868.5
18	1854.0
19	1808.5
20	1763.0
21	1726.5
22	1690.0
23	1969.0
24	2248.0
25	2785.0
26	4022.5
27	4723.0
28	5852.0
29	6981.0
30	7725.5
31	8470.0
32	9642.0
33	10814.0
34	12670.0
35	14526.0
36	14729.5
37	14933.0
38	16267.5
39	17602.0
40	19912.0
41	22222.0
42	24915.0
43	27608.0
44	31217.5
45	34827.0
46	65659.5
47	96492.0
48	74042.5
49	51593.0
50	50203.5
51	48814.0
52	44057.5
53	39301.0
54	37905.0
55	36509.0
56	34711.5
57	32914.0
58	31001.5
59	29089.0
60	27802.5
61	26516.0
62	23795.5
63	21075.0
64	18210.0
65	15345.0
66	12644.5
67	9944.0
68	8383.0
69	6822.0
70	5625.5
71	4429.0
72	3722.0
73	3015.0
74	2650.5
75	1799.5
76	1313.0
77	994.0
78	675.0
79	523.5
80	372.0
81	301.5
82	231.0
83	150.5
84	70.0
85	48.0
86	26.0
87	15.0
88	4.0
89	4.5
90	5.0
91	2.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	626339.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.77996632711752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.42910144743212	19.445596953995313
2	8.807022047145052	4.540894636351622
3	3.1933460117310783	2.469730579597867
4	1.6821824385376156	1.7346642648627273
5	1.0192577588900602	1.3138215351419513
6	0.7443372282088423	1.1513393207546367
7	0.6261820089258774	1.1300065773329147
8	0.5040005448795525	1.0394493660674995
9	0.4796778322786689	1.1129470527607928
>10	6.766556746090176	40.54364391271417
>50	0.6760570278358413	10.903958023129734
>100	0.059193933312815604	2.7530963974959195
>500	0.0068540343835891745	1.145477006274777
>1k	0.0037385642092304587	2.947462850671138
>5k	6.230940348717431E-4	0.8356151152210615
>10k+	0.0018692821046152293	6.932296407627861
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCG	15713	2.5087053496588907	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTC	14252	2.2754450864467963	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGC	13191	2.106048002758889	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5202	0.8305406497120569	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGTCGATT	3978	0.6351193203680435	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC	3750	0.5987173080392567	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCT	3134	0.500368011572008	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCT	2811	0.44879849410622685	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTA	2526	0.4032959786952433	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTC	2150	0.34326458994250714	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCGTCGAT	812	0.12964225443410038	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATG	781	0.12469285802097585	No Hit
CCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC	771	0.12309627853287117	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTT	735	0.11734859237569431	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	651	0.10393732467561496	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.193158976209369E-4	0.0	0.0	0.08190452773977032	0.0
2	3.193158976209369E-4	0.0	0.0	0.8886561430790674	0.0
3	3.193158976209369E-4	0.0	0.0	1.042566405732359	0.0
4	3.193158976209369E-4	0.0	0.0	1.6668289855812906	0.0
5	3.193158976209369E-4	0.0	0.0	4.516244398001721	0.0
6	3.193158976209369E-4	0.0	0.0	4.941253857735188	0.0
7	3.193158976209369E-4	0.0	0.0	5.728367545370798	0.0
8	3.193158976209369E-4	0.0	0.0	6.4455510514274215	0.0
9	3.193158976209369E-4	0.0	0.0	6.6187799258867805	0.0
10	3.193158976209369E-4	0.0	0.0	9.624181154295039	0.0
11	3.193158976209369E-4	0.0	0.0	10.23790630952248	0.0
12	3.193158976209369E-4	0.0	0.0	13.003820614715034	0.0
13	3.193158976209369E-4	0.0	0.0	13.385243454423243	0.0
14	3.193158976209369E-4	0.0	0.0	13.573959149917217	0.0
15	3.193158976209369E-4	0.0	0.0	14.175230985137441	0.0
16	3.193158976209369E-4	0.0	0.0	14.601517708461392	0.0
17	3.193158976209369E-4	0.0	0.0	15.022216403576977	0.0
18	3.193158976209369E-4	0.0	0.0	15.467342764860563	0.0
19	3.193158976209369E-4	0.0	0.0	16.416349612589986	0.0
20	3.193158976209369E-4	0.0	0.0	16.799209373837492	0.0
21	3.193158976209369E-4	0.0	0.0	17.08882889297968	0.0
22	3.193158976209369E-4	0.0	0.0	17.470730706534322	0.0
23	3.193158976209369E-4	0.0	0.0	17.774559783120644	0.0
24	3.193158976209369E-4	0.0	0.0	18.033844291988842	0.0
25	3.193158976209369E-4	0.0	0.0	18.269020450586662	0.0
26	3.193158976209369E-4	0.0	0.0	18.48950807789392	0.0
27	3.193158976209369E-4	0.0	0.0	18.784715625244477	0.0
28	3.193158976209369E-4	0.0	0.0	19.022126995125642	0.0
29	3.193158976209369E-4	0.0	0.0	19.24500949166506	0.0
30	3.193158976209369E-4	0.0	0.0	19.50173947335229	0.0
31	4.789738464314054E-4	0.0	0.0	19.731806577588173	0.0
32	4.789738464314054E-4	0.0	0.0	19.98646100594087	0.0
33	4.789738464314054E-4	0.0	0.0	20.23472911634115	0.0
34	4.789738464314054E-4	0.0	0.0	20.474216039556854	0.0
35	4.789738464314054E-4	0.0	0.0	20.725836966882152	0.0
36	4.789738464314054E-4	0.0	0.0	20.985440791647974	0.0
37	4.789738464314054E-4	0.0	0.0	21.242490089232827	0.0
38	4.789738464314054E-4	0.0	0.0	21.470641298082988	0.0
39	4.789738464314054E-4	0.0	0.0	21.72785025361665	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATGCG	30	2.1639135E-6	45.000004	1
CTCGAGT	30	2.1639135E-6	45.000004	18
CGATACC	30	2.1639135E-6	45.000004	34
TAGTCGG	30	2.1639135E-6	45.000004	2
TTGGACG	20	7.030825E-4	45.0	1
CGTTGAT	20	7.030825E-4	45.0	25
ATAGCCG	25	3.888765E-5	45.0	1
CATTACG	25	3.888765E-5	45.0	35
CGTTACC	20	7.030825E-4	45.0	41
TACGACG	20	7.030825E-4	45.0	1
CTCTCGA	20	7.030825E-4	45.0	11
AGAATCG	25	3.888765E-5	45.0	28
TATCCGA	25	3.888765E-5	45.0	12
GATGAAT	2235	0.0	42.684563	20
CGATGAA	2175	0.0	42.517242	19
CGATCGA	85	0.0	42.35294	41
CCGATGA	2185	0.0	42.21968	18
CGTTTTT	2400	0.0	42.1875	1
TACGGCT	1445	0.0	41.730103	7
TGATACC	1850	0.0	41.472973	4
>>END_MODULE