FastQCFastQC Report
Sat 18 Jun 2016
SRR3551883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763032
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC109741.4382096687950179No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCG82521.081474957799935No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC65350.8564516298136907No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTT38100.4993237505111188No Hit
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC33050.4331404187504587TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.4099434886086036No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT26080.3417943153104981TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCCTTTT20250.26538860755512217No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCT17110.2242369913712662No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTA15750.20641336143176173No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTC14020.183740655699892No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC9580.12555174619150966No Hit
CCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC7950.10418960148460353TruSeq Adapter, Index 23 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAA207.031793E-445.00000420
AACGGTC207.031793E-445.00000435
TCCCGAT207.031793E-445.00000441
ATGCGAG207.031793E-445.0000041
TTACGCG207.031793E-445.0000041
TCGAACC207.031793E-445.00000414
ATTCCGT253.8895683E-545.012
ACCTAAC253.8895683E-545.045
CGAATAC502.1827873E-1145.045
CACGTAG253.8895683E-545.01
GCATCGT453.8380676E-1045.035
GGCGAAC2100.043.9285732
CGAATGC1600.043.59375445
CTTCGAA1000.042.7542
TACGCGG650.041.5384642
CGGCGAA2450.041.3265331
CCGATGA13100.041.04961818
CGGTCTA556.002665E-1140.90909231
CGTTTTT17300.040.7080961
CGCATCG501.0804797E-940.521