##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551883_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	763032
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.201505310393273	28.0	25.0	31.0	16.0	33.0
2	27.504448044118725	30.0	25.0	33.0	16.0	33.0
3	27.44226061292318	30.0	25.0	33.0	16.0	34.0
4	31.593853206680716	35.0	28.0	35.0	19.0	37.0
5	33.04553937449543	35.0	32.0	35.0	28.0	37.0
6	32.754898877111316	35.0	32.0	35.0	28.0	37.0
7	33.88411233080657	35.0	33.0	37.0	30.0	37.0
8	33.92301240314954	35.0	35.0	37.0	30.0	37.0
9	36.075941769152536	39.0	35.0	39.0	30.0	39.0
10	35.17797942943415	37.0	34.0	39.0	30.0	39.0
11	35.75737321632644	37.0	35.0	39.0	31.0	39.0
12	35.767385902557166	37.0	35.0	39.0	31.0	39.0
13	35.88628786210801	37.0	35.0	39.0	31.0	39.0
14	36.811571467513815	38.0	36.0	40.0	31.0	41.0
15	36.980555206072616	39.0	36.0	40.0	32.0	41.0
16	36.55070036381174	38.0	35.0	40.0	31.0	41.0
17	36.65858836850879	38.0	35.0	40.0	31.0	41.0
18	36.42757446607744	38.0	35.0	40.0	31.0	41.0
19	36.03818319546232	38.0	34.0	40.0	30.0	41.0
20	36.3282706360939	38.0	35.0	40.0	31.0	41.0
21	36.52387973243586	38.0	35.0	40.0	31.0	41.0
22	36.88632062613364	39.0	35.0	40.0	31.0	41.0
23	37.210216609526206	39.0	35.0	40.0	32.0	41.0
24	37.29303227125468	39.0	35.0	40.0	32.0	41.0
25	36.276496922802714	38.0	35.0	40.0	30.0	41.0
26	36.550957233772635	38.0	35.0	40.0	31.0	41.0
27	36.75408239759276	39.0	35.0	40.0	31.0	41.0
28	36.730834355570934	39.0	35.0	40.0	31.0	41.0
29	36.75650562492792	39.0	35.0	40.0	31.0	41.0
30	36.39004261944453	39.0	35.0	40.0	30.0	41.0
31	36.57243601841076	39.0	35.0	40.0	31.0	41.0
32	36.479197464850756	39.0	35.0	40.0	31.0	41.0
33	36.53765372880823	39.0	35.0	40.0	31.0	41.0
34	36.56134342989547	39.0	35.0	41.0	31.0	41.0
35	36.47002484823703	39.0	35.0	41.0	30.0	41.0
36	36.31217825726837	39.0	35.0	41.0	30.0	41.0
37	36.355576175049016	39.0	35.0	41.0	30.0	41.0
38	36.21634348231791	39.0	35.0	40.0	30.0	41.0
39	36.056386888099055	39.0	35.0	40.0	30.0	41.0
40	35.95730454292874	39.0	35.0	40.0	29.0	41.0
41	35.72269708216693	38.0	35.0	40.0	28.0	41.0
42	35.90971807211231	39.0	35.0	40.0	29.0	41.0
43	35.902032942261926	39.0	35.0	40.0	30.0	41.0
44	36.012025707965066	39.0	35.0	40.0	30.0	41.0
45	36.044391323037566	39.0	35.0	40.0	30.0	41.0
46	35.855239360865596	38.0	35.0	40.0	29.0	41.0
47	35.78338261042787	38.0	35.0	40.0	29.0	41.0
48	35.801844483586535	38.0	35.0	40.0	30.0	41.0
49	35.742061931871795	38.0	35.0	40.0	29.0	41.0
50	35.59721348514872	38.0	35.0	40.0	29.0	41.0
51	34.69769419893268	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	35.0
10	45.0
11	38.0
12	35.0
13	69.0
14	88.0
15	114.0
16	161.0
17	271.0
18	480.0
19	976.0
20	1838.0
21	3100.0
22	4401.0
23	5444.0
24	6676.0
25	7717.0
26	8680.0
27	9496.0
28	10535.0
29	12603.0
30	16261.0
31	21846.0
32	31091.0
33	47615.0
34	62814.0
35	67460.0
36	94819.0
37	140688.0
38	160225.0
39	47397.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.398971471707604	18.007370595204396	20.07347005105946	15.520187882028539
2	31.251638201281207	28.158321014059702	22.913980016565493	17.676060768093606
3	32.15173151322618	26.232189475670747	24.977982574780615	16.63809643632246
4	28.26015160569937	22.917780643537885	29.8343975088856	18.98767024187714
5	29.14255234380734	27.15875612031999	23.019873347382546	20.678818188490126
6	24.681533670933852	37.06725274955703	23.114495853384916	15.1367177261242
7	74.15390180228351	6.126872791704673	15.009200138395245	4.710025267616561
8	73.98510154226821	10.10612923180155	10.092106228834439	5.816662997095796
9	67.58196248650123	8.275799704337434	11.658226653665901	12.484011155495445
10	41.98788517388524	23.70215141697858	18.84206691200369	15.467896497132491
11	32.82784994600488	24.931850826701897	23.341222910703614	18.899076316589607
12	27.671971817695717	21.208546954780402	28.845448159448097	22.27403306807578
13	26.847628932993633	22.77768166996928	30.87590035542415	19.498789041612934
14	20.957181350192393	29.40178131454513	27.634751884586755	22.006285450675726
15	20.324573543442476	24.791358684825802	33.31393703016387	21.57013074156785
16	22.4712725023328	24.309596452049192	29.408858344079935	23.810272701538075
17	22.053465647574413	25.965621363193154	28.046792270835297	23.934120718397132
18	22.88383711299133	25.150976630075807	27.396360834145884	24.568825422786986
19	24.731203933779973	25.922897073779342	25.58084064626385	23.765058346176833
20	26.986548401639777	25.19239035846465	27.888607555122196	19.932453684773378
21	26.290509441281625	26.967807378982794	26.9928390945596	19.748844085175982
22	23.1798928485306	23.555237526080163	29.34110233908932	23.923767286299917
23	24.46935384099225	26.139139642898336	27.39544344142841	21.99606307468101
24	23.562314555614968	25.93456106690152	27.57407290913094	22.929051468352572
25	22.854742658237136	28.74361756780843	26.06915568416528	22.332484089789155
26	21.80288637960138	25.55908533324946	28.22083477495046	24.4171935121987
27	25.606658698455636	26.08239235051741	25.688306650310867	22.622642300716088
28	21.919657366925634	27.64851277534887	29.004026043468688	21.42780381425681
29	22.989861499910884	24.800794724205538	27.66620534918588	24.5431384266977
30	22.51570052107906	28.71478522525923	26.144512943100683	22.625001310561025
31	25.954350538378467	25.225940720703722	23.827834219272585	24.991874521645226
32	25.55200830371465	26.738983424024156	25.064086434120718	22.644921838140473
33	23.960594051101396	24.68100944652387	25.12620702670399	26.232189475670747
34	22.51111355749169	24.756497761561768	27.51339393367513	25.218994747271413
35	23.04280816531941	25.38530494133929	27.99423877373426	23.57764811960704
36	23.366123570177923	26.859292926115813	27.058105033602786	22.716478470103482
37	22.585946592017113	27.23214753771795	27.751653928013504	22.430251942251438
38	23.370972645970287	25.083351681187683	25.366432862579813	26.179242810262217
39	23.5827593076044	24.11052223235723	27.55506977426897	24.751648685769403
40	22.216630495182375	23.871082733096383	29.489064678807704	24.423222092913534
41	20.091686849306452	25.372068274987157	29.761530315897627	24.774714559808764
42	21.906420700573502	24.291903878212185	29.9999213663385	23.80175405487581
43	23.430865284811123	23.7914006227786	27.776030363077826	25.00170372933245
44	23.0935268769855	24.418241961018673	27.08877216158693	25.399459000408896
45	22.643480221013014	23.661392969102213	26.64711309617421	27.048013713710567
46	24.047615303158977	26.356037492529804	25.904811331634846	23.691535872676376
47	21.74351796517053	24.67773304396146	30.27160066681345	23.30714832405456
48	22.18557019889074	24.880345778420825	27.639732016481616	25.294352006206815
49	21.654661927678003	23.809486364923096	30.730165969448205	23.805685737950704
50	21.64968179578314	23.904108870925466	29.78027133855461	24.665937994736787
51	21.526358003334067	24.891223434928026	27.137525031715576	26.444893530022334
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	242.0
1	902.0
2	1562.0
3	3772.0
4	5982.0
5	4252.5
6	2523.0
7	2542.5
8	2562.0
9	2498.0
10	2434.0
11	2350.0
12	2266.0
13	2187.0
14	2108.0
15	2163.0
16	2218.0
17	2045.5
18	1873.0
19	1879.0
20	1885.0
21	1995.0
22	2105.0
23	2351.0
24	2597.0
25	3034.0
26	4810.0
27	6149.0
28	6743.0
29	7337.0
30	8045.0
31	8753.0
32	10757.5
33	12762.0
34	14417.5
35	16073.0
36	17148.5
37	18224.0
38	19368.0
39	20512.0
40	23195.5
41	25879.0
42	30225.5
43	34572.0
44	38823.5
45	43075.0
46	66564.5
47	90054.0
48	77604.5
49	65155.0
50	63651.0
51	62147.0
52	56810.0
53	51473.0
54	48246.0
55	45019.0
56	44119.5
57	43220.0
58	42359.5
59	41499.0
60	39837.5
61	38176.0
62	34414.5
63	30653.0
64	26819.0
65	22985.0
66	19070.0
67	15155.0
68	12917.0
69	10679.0
70	8896.0
71	7113.0
72	5990.5
73	4868.0
74	4062.5
75	2790.0
76	2323.0
77	1725.0
78	1127.0
79	880.5
80	634.0
81	420.5
82	207.0
83	126.5
84	46.0
85	48.5
86	51.0
87	30.5
88	10.0
89	11.5
90	13.0
91	7.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	763032.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.53033218234333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67283186295785	20.076253665028865
2	9.25858097468676	4.912664575911276
3	3.165780534654888	2.51967627602372
4	1.582981522935236	1.6798810256793417
5	1.0222233732119284	1.3559962827933987
6	0.6846608535599702	1.0898567926315639
7	0.5760838762450611	1.0698587621171407
8	0.40740882340759693	0.8646953135216959
9	0.3984668214694066	0.9514311423503264
>10	6.264700251804513	40.73040380372233
>50	0.8940218166529009	15.06309056259816
>100	0.06279082993207871	2.970522054911419
>500	0.003986719360766902	0.7231954620066522
>1k	0.003986719360766902	2.58658062499052
>5k	9.966798401917255E-4	1.9550075496695365
>10k+	4.983399200958628E-4	1.4508861060440585
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC	10974	1.4382096687950179	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCG	8252	1.081474957799935	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC	6535	0.8564516298136907	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTT	3810	0.4993237505111188	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	3305	0.4331404187504587	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3128	0.4099434886086036	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT	2608	0.3417943153104981	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCCTTTT	2025	0.26538860755512217	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCT	1711	0.2242369913712662	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTA	1575	0.20641336143176173	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTC	1402	0.183740655699892	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	958	0.12555174619150966	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	795	0.10418960148460353	TruSeq Adapter, Index 23 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0809926713427484	0.0
2	0.0	0.0	0.0	0.7246091907023559	0.0
3	0.0	0.0	0.0	0.8809591209805093	0.0
4	0.0	0.0	0.0	1.4296910221327546	0.0
5	0.0	0.0	0.0	3.423447509409828	0.0
6	0.0	0.0	0.0	4.370196793843508	0.0
7	0.0	0.0	0.0	4.942649849547594	0.0
8	0.0	0.0	0.0	5.608021681921597	0.0
9	0.0	0.0	0.0	5.823608970528103	0.0
10	0.0	0.0	0.0	7.386846161104645	0.0
11	0.0	0.0	0.0	8.006348357604924	0.0
12	0.0	0.0	0.0	9.441805848247517	0.0
13	0.0	0.0	0.0	9.83602260455656	0.0
14	0.0	0.0	0.0	10.015045240566582	0.0
15	0.0	0.0	0.0	10.411358894515564	0.0
16	0.0	0.0	0.0	10.787883076987598	0.0
17	0.0	0.0	0.0	11.200054519338638	0.0
18	0.0	0.0	0.0	11.67159437612053	0.0
19	0.0	0.0	0.0	12.220064165067782	0.0
20	0.0	0.0	0.0	12.545345411463739	0.0
21	0.0	0.0	0.0	12.85214774740771	0.0
22	0.0	0.0	0.0	13.224216022394867	0.0
23	0.0	0.0	0.0	13.540061229411087	0.0
24	0.0	0.0	0.0	13.816065381268414	0.0
25	0.0	0.0	0.0	14.063106134474046	0.0
26	0.0	0.0	0.0	14.401624047222136	0.0
27	0.0	0.0	0.0	14.686408957946718	0.0
28	0.0	0.0	0.0	14.946948489709476	0.0
29	0.0	0.0	0.0	15.197003533272524	0.0
30	0.0	0.0	0.0	15.482050556202099	0.0
31	0.0	0.0	0.0	15.77443672087147	0.0
32	0.0	0.0	0.0	16.057648958366098	0.0
33	0.0	0.0	0.0	16.361305947850155	0.0
34	0.0	0.0	0.0	16.665225049539206	0.0
35	0.0	0.0	0.0	16.961018672873482	0.0
36	0.0	0.0	0.0	17.240692395600707	0.0
37	0.0	0.0	0.0	17.535699682320008	0.0
38	0.0	0.0	0.0	17.825726837144444	0.0
39	0.0	0.0	0.0	18.144717390620578	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAA	20	7.031793E-4	45.000004	20
AACGGTC	20	7.031793E-4	45.000004	35
TCCCGAT	20	7.031793E-4	45.000004	41
ATGCGAG	20	7.031793E-4	45.000004	1
TTACGCG	20	7.031793E-4	45.000004	1
TCGAACC	20	7.031793E-4	45.000004	14
ATTCCGT	25	3.8895683E-5	45.0	12
ACCTAAC	25	3.8895683E-5	45.0	45
CGAATAC	50	2.1827873E-11	45.0	45
CACGTAG	25	3.8895683E-5	45.0	1
GCATCGT	45	3.8380676E-10	45.0	35
GGCGAAC	210	0.0	43.92857	32
CGAATGC	160	0.0	43.593754	45
CTTCGAA	100	0.0	42.75	42
TACGCGG	65	0.0	41.538464	2
CGGCGAA	245	0.0	41.32653	31
CCGATGA	1310	0.0	41.049618	18
CGGTCTA	55	6.002665E-11	40.909092	31
CGTTTTT	1730	0.0	40.708096	1
CGCATCG	50	1.0804797E-9	40.5	21
>>END_MODULE