FastQCFastQC Report
Sat 18 Jun 2016
SRR3551880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561707
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC232464.1384565262672535No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG232034.1308012896403286No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC221123.9365719138269597No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGAACACT63221.1254978129166986No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58631.0437826126432463No Hit
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC55100.9809384607989574TruSeq Adapter, Index 19 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT53980.9609992398172TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCT48160.8573865022155678No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTA46010.8191103190809443No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTC37850.6738388519281405No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATG13970.24870617599567035No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT12520.22289200597464515No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTGAACAC11330.2017065836815279No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGAACACTCGT10910.1942293758133689No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTAT8610.15328276129725996No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCAACCCTCGAATGC7400.13174128148661135No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT5910.10521499643052339No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCTGAACACT5860.10432485263669493No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAA207.0302014E-445.00000425
AACGTCC207.0302014E-445.00000432
CTATCCC453.8380676E-1045.00000434
GTTACCG207.0302014E-445.00000438
CGGTGGT207.0302014E-445.00000437
GAACGAA207.0302014E-445.00000436
CGATCGA207.0302014E-445.00000441
CGTCAAT207.0302014E-445.00000436
TCGAAAG207.0302014E-445.00000444
GTACGAG207.0302014E-445.0000041
GCGAACC2550.045.033
TCGTGTA750.045.017
TACACGC351.2104465E-745.035
TTTAGCG302.1635133E-644.9999961
TCCGAAT302.1635133E-644.99999631
ACCGCGA302.1635133E-644.99999630
GTAATCG253.8882506E-544.9999961
ACGATCA253.8882506E-544.99999640
CCCGTAA253.8882506E-544.99999641
AATTGCG302.1635133E-644.9999961
Produced by FastQC (version 0.11.5)