##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551880_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	561707
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.744363164425582	30.0	25.0	31.0	16.0	33.0
2	27.594692606643676	30.0	25.0	31.0	16.0	33.0
3	27.500645354250526	30.0	25.0	31.0	16.0	33.0
4	31.99341293592567	35.0	32.0	35.0	19.0	37.0
5	33.29447736987433	35.0	32.0	35.0	28.0	37.0
6	33.23398497793333	35.0	33.0	35.0	28.0	37.0
7	33.783549074517495	35.0	33.0	35.0	30.0	37.0
8	33.34787887635368	35.0	33.0	37.0	28.0	37.0
9	35.345265414175	37.0	34.0	39.0	28.0	39.0
10	34.79184343438839	35.0	33.0	38.0	30.0	39.0
11	35.606730911311416	37.0	35.0	39.0	31.0	39.0
12	35.652446916274855	37.0	35.0	39.0	31.0	39.0
13	35.35351882743139	37.0	34.0	39.0	30.0	39.0
14	36.44038083912075	38.0	35.0	40.0	30.0	41.0
15	36.429448093044954	38.0	35.0	40.0	30.0	41.0
16	36.316082940038136	38.0	34.0	40.0	31.0	41.0
17	35.765854796183774	37.0	34.0	40.0	29.0	41.0
18	35.97275981962126	38.0	34.0	39.0	30.0	41.0
19	35.58082060576066	37.0	34.0	40.0	29.0	41.0
20	34.810636150163695	37.0	33.0	39.0	26.0	41.0
21	35.70135853745814	38.0	34.0	40.0	30.0	41.0
22	35.99830694650414	38.0	34.0	40.0	30.0	41.0
23	36.06093568355032	38.0	34.0	40.0	30.0	41.0
24	36.19167822370026	38.0	35.0	40.0	30.0	41.0
25	35.81443706416334	37.0	34.0	40.0	30.0	41.0
26	35.59138305201822	38.0	34.0	40.0	27.0	41.0
27	35.4095800835667	38.0	34.0	40.0	27.0	41.0
28	35.274178530799865	38.0	34.0	40.0	25.0	41.0
29	35.583017480643825	38.0	34.0	40.0	27.0	41.0
30	35.08296140158481	38.0	34.0	40.0	24.0	41.0
31	35.241766614978985	38.0	34.0	40.0	25.0	41.0
32	34.57458069776592	38.0	34.0	40.0	22.0	41.0
33	34.39880400279861	38.0	33.0	40.0	22.0	41.0
34	34.56419628026711	38.0	34.0	40.0	22.0	41.0
35	34.09737639018207	38.0	33.0	40.0	18.0	41.0
36	34.04031461242249	38.0	33.0	40.0	18.0	41.0
37	33.78935815291602	38.0	34.0	40.0	12.0	41.0
38	33.82958375095913	38.0	33.0	40.0	14.0	41.0
39	33.828050923346154	38.0	33.0	40.0	13.0	41.0
40	33.51212464861574	37.0	33.0	40.0	15.0	41.0
41	33.836872248343	38.0	33.0	40.0	20.0	41.0
42	33.42351439451529	38.0	33.0	40.0	11.0	41.0
43	33.61855914204381	38.0	33.0	40.0	12.0	41.0
44	34.08495176310781	38.0	34.0	40.0	17.0	41.0
45	34.17603483666751	38.0	34.0	40.0	18.0	41.0
46	33.96078204473151	38.0	34.0	40.0	17.0	41.0
47	33.62905749794822	37.0	33.0	40.0	18.0	41.0
48	33.72340917951886	37.0	33.0	40.0	18.0	41.0
49	33.78990826178061	37.0	34.0	40.0	15.0	41.0
50	33.57188890293338	37.0	33.0	40.0	14.0	41.0
51	32.90160172474261	36.0	32.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	12.0
10	21.0
11	15.0
12	26.0
13	33.0
14	44.0
15	54.0
16	90.0
17	203.0
18	499.0
19	1602.0
20	4001.0
21	6976.0
22	8920.0
23	10009.0
24	11144.0
25	11982.0
26	11643.0
27	10692.0
28	10215.0
29	11419.0
30	14244.0
31	18346.0
32	24602.0
33	34902.0
34	45881.0
35	48927.0
36	63274.0
37	87828.0
38	96783.0
39	27312.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	56.93644551340824	12.926668173976823	14.452018579081265	15.684867733533675
2	25.388681287575192	43.680958221991176	16.786509692775773	14.143850797657853
3	22.70329549035351	43.01050191647959	21.61963443574675	12.66656815742015
4	20.833637465796226	16.741290388049286	47.59527654097243	14.829795605182062
5	40.450448365428954	20.109772532654922	21.308618194183087	18.131160907733033
6	18.23673196168109	45.615774772256714	24.201941581643098	11.945551684419101
7	56.248186332020076	5.12064118837757	34.0791551467224	4.552017332879953
8	52.57794544130658	25.440309627617246	15.515918441465034	6.465826489611132
9	49.86834773289277	6.150003471560796	13.775153238254106	30.206495557292325
10	45.124593426822166	17.542419802494898	22.02037717172832	15.312609598954616
11	38.453143720836664	19.376828132816577	24.80296667123607	17.367061475110688
12	24.41593214967946	15.894407582600895	29.350355256388116	30.33930501133153
13	29.92681237727142	19.734665937935613	35.79784478384639	14.540676900946577
14	18.33642806658988	35.738561207177405	24.71662272323471	21.208388002998007
15	13.754501902237287	20.44126208147664	39.57205446967903	26.232181546607038
16	15.217008155497439	21.760455183930414	27.46894733375229	35.55358932681985
17	15.713174306177418	30.56504547744643	32.14505071149193	21.576729504884216
18	15.73008703826016	22.285461993530433	30.352835196997724	31.63161577121168
19	17.660630898315315	24.815606713108433	25.40933262359201	32.11442976498424
20	27.731717781690456	22.42699485674916	34.772399133356004	15.068888228204383
21	20.536151409898757	35.931722410438184	26.70662818871761	16.825497990945458
22	16.860035570146	18.691773469086197	38.45260963456037	25.995581326207436
23	25.88021869052727	27.776403000140643	28.691114762678765	17.652263546653327
24	21.505696030136708	28.7391825275455	25.364647405141827	24.390474037175967
25	15.79293119010445	39.87452533082194	24.342228243550462	19.990315235523145
26	16.77155527703945	19.899876626070174	37.71200999809509	25.616558098795277
27	28.640198537671775	24.416466235955756	25.715898146186532	21.227437080185933
28	15.892627295013236	30.527125351829337	32.00476404958457	21.57548330357286
29	20.68213499208662	20.256824287395386	33.59491692287972	25.466123797638268
30	16.939970482831797	34.372190483650726	31.355493166366095	17.332345867151382
31	25.138194823991867	20.771149371469466	21.3184097759152	32.772246028623464
32	29.07832731299414	31.771546375601517	22.012365877583864	17.13776043382048
33	25.325659106972143	25.14122131289088	21.75351206233855	27.77960751779843
34	21.34778452111154	22.326230579287778	22.10956957986993	34.21641531973075
35	23.194298807029288	26.36356677057612	26.845490620554845	23.596643801839747
36	20.99297320489152	28.368348623036567	21.368613885887125	29.270064286184788
37	16.866266576702802	27.401296405421334	32.88244583029943	22.84999118757644
38	22.797650732499328	26.73084009990974	20.978196817913965	29.493312349676966
39	20.6500898155088	29.456282367853703	23.125401677386964	26.768226139250533
40	17.047855910643804	30.86484590720785	27.362308107251643	24.7249900748967
41	16.999610117018303	24.22615349283523	22.137164037478616	36.63707235266785
42	19.5484478562667	26.31069222922271	30.51679968382092	23.62406023068967
43	21.0755785489588	23.669635592933684	22.408123808319996	32.846662049787525
44	20.85624711815947	20.609677287268983	31.50201083483026	27.032064759741285
45	20.766342594982792	20.90271262419731	27.37263377526006	30.958311005559835
46	27.845300129782963	27.805243659060686	22.70080308773079	21.648653123425557
47	15.313321713989678	27.34966806537928	36.93277812097766	20.404232099653377
48	22.179178824547318	25.311772863788416	24.90640138007894	27.60264693158533
49	19.43486550817419	22.82435504631418	35.224058094344564	22.516721351167067
50	21.418461938341522	22.97977415271663	28.46145766387102	27.14030624507083
51	19.36543429225557	20.688187969884655	27.02298529304424	32.92339244481553
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	173.0
1	543.5
2	914.0
3	5166.0
4	9418.0
5	6384.5
6	3351.0
7	3250.0
8	3149.0
9	3446.5
10	3744.0
11	3273.0
12	2802.0
13	2643.5
14	2485.0
15	2316.0
16	2147.0
17	2037.0
18	1927.0
19	1770.5
20	1614.0
21	1628.5
22	1643.0
23	1605.0
24	1567.0
25	2094.0
26	2973.5
27	3326.0
28	3928.0
29	4530.0
30	5305.0
31	6080.0
32	7001.0
33	7922.0
34	8744.0
35	9566.0
36	10182.0
37	10798.0
38	12217.0
39	13636.0
40	14946.0
41	16256.0
42	18110.5
43	19965.0
44	23077.0
45	26189.0
46	74021.0
47	121853.0
48	84233.0
49	46613.0
50	46148.5
51	45684.0
52	40725.5
53	35767.0
54	34154.5
55	32542.0
56	31005.5
57	29469.0
58	26465.0
59	23461.0
60	22072.0
61	20683.0
62	18015.0
63	15347.0
64	13227.0
65	11107.0
66	9147.0
67	7187.0
68	6231.0
69	5275.0
70	4427.5
71	3580.0
72	3112.0
73	2644.0
74	2169.0
75	1537.0
76	1380.0
77	1055.0
78	730.0
79	590.0
80	450.0
81	363.0
82	276.0
83	188.5
84	101.0
85	61.5
86	22.0
87	13.5
88	5.0
89	6.0
90	7.0
91	5.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	561707.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.11004813265676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0979556552199	19.86921284954572
2	9.105615272539954	4.754961060869454
3	3.26931697036393	2.560860703713414
4	1.6826062610536527	1.75731721857682
5	1.0709316315219908	1.3981038222911908
6	0.7759749177539588	1.2156445471374142
7	0.5916829354663105	1.0814208947007908
8	0.5630548641895975	1.1761111684253525
9	0.46345519438028626	1.0890753689339163
>10	5.800898533900119	33.511658340754366
>50	0.5031098323340609	8.21676051265903
>100	0.06237465223760155	3.136586181060772
>500	0.0034271786943737116	0.5943514040455794
>1k	0.004798050172123196	3.234839394195679
>5k	0.002741742955498969	4.132898817712907
>10k+	0.002056307216624227	12.270197715377588
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	23246	4.1384565262672535	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG	23203	4.1308012896403286	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC	22112	3.9365719138269597	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGAACACT	6322	1.1254978129166986	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5863	1.0437826126432463	No Hit
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	5510	0.9809384607989574	TruSeq Adapter, Index 19 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT	5398	0.9609992398172	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCT	4816	0.8573865022155678	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTA	4601	0.8191103190809443	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTC	3785	0.6738388519281405	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATG	1397	0.24870617599567035	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT	1252	0.22289200597464515	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTGAACAC	1133	0.2017065836815279	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGAACACTCGT	1091	0.1942293758133689	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTAT	861	0.15328276129725996	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCAACCCTCGAATGC	740	0.13174128148661135	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	591	0.10521499643052339	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCTGAACACT	586	0.10432485263669493	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10325668008410079	0.0
2	0.0	0.0	0.0	1.2882160984285402	0.0
3	0.0	0.0	0.0	1.4463056362124738	0.0
4	0.0	0.0	0.0	2.636605917319884	0.0
5	0.0	0.0	0.0	7.4355491386078505	0.0
6	0.0	0.0	0.0	7.959131718137748	0.0
7	0.0	0.0	0.0	9.145693395311078	0.0
8	0.0	0.0	0.0	10.24003617544378	0.0
9	0.0	0.0	0.0	10.440140500296417	0.0
10	0.0	0.0	0.0	15.070846544550808	0.0
11	0.0	0.0	0.0	15.642318860188675	0.0
12	0.0	0.0	0.0	20.27320293320183	0.0
13	0.0	0.0	0.0	20.760467645943525	0.0
14	0.0	0.0	0.0	21.017897231118717	0.0
15	0.0	0.0	0.0	22.035865673740936	0.0
16	1.78028758765691E-4	0.0	0.0	22.53060759435079	0.0
17	1.78028758765691E-4	0.0	0.0	22.942032055858302	0.0
18	1.78028758765691E-4	0.0	0.0	23.35132017226063	0.0
19	1.78028758765691E-4	0.0	0.0	24.82735661118697	0.0
20	1.78028758765691E-4	0.0	0.0	25.289519268942705	0.0
21	1.78028758765691E-4	0.0	0.0	25.575077398002872	0.0
22	1.78028758765691E-4	0.0	0.0	26.037418084517373	0.0
23	1.78028758765691E-4	0.0	0.0	26.331877651515825	0.0
24	1.78028758765691E-4	0.0	0.0	26.59998896221696	0.0
25	1.78028758765691E-4	0.0	0.0	26.814157559012084	0.0
26	1.78028758765691E-4	0.0	0.0	27.093840739032984	0.0
27	1.78028758765691E-4	0.0	0.0	27.473754110238968	0.0
28	1.78028758765691E-4	0.0	0.0	27.692017368485704	0.0
29	1.78028758765691E-4	0.0	0.0	27.910636684249972	0.0
30	1.78028758765691E-4	0.0	0.0	28.149195220996	0.0
31	1.78028758765691E-4	0.0	0.0	28.383303038772883	0.0
32	1.78028758765691E-4	0.0	0.0	28.660137758653534	0.0
33	1.78028758765691E-4	0.0	0.0	28.91115830851316	0.0
34	1.78028758765691E-4	0.0	0.0	29.167875778653283	0.0
35	1.78028758765691E-4	0.0	0.0	29.4204985873418	0.0
36	1.78028758765691E-4	0.0	0.0	29.66350784305697	0.0
37	1.78028758765691E-4	0.0	0.0	29.901888351044228	0.0
38	1.78028758765691E-4	0.0	0.0	30.140446887790254	0.0
39	1.78028758765691E-4	0.0	0.0	30.375978935637264	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAA	20	7.0302014E-4	45.000004	25
AACGTCC	20	7.0302014E-4	45.000004	32
CTATCCC	45	3.8380676E-10	45.000004	34
GTTACCG	20	7.0302014E-4	45.000004	38
CGGTGGT	20	7.0302014E-4	45.000004	37
GAACGAA	20	7.0302014E-4	45.000004	36
CGATCGA	20	7.0302014E-4	45.000004	41
CGTCAAT	20	7.0302014E-4	45.000004	36
TCGAAAG	20	7.0302014E-4	45.000004	44
GTACGAG	20	7.0302014E-4	45.000004	1
GCGAACC	255	0.0	45.0	33
TCGTGTA	75	0.0	45.0	17
TACACGC	35	1.2104465E-7	45.0	35
TTTAGCG	30	2.1635133E-6	44.999996	1
TCCGAAT	30	2.1635133E-6	44.999996	31
ACCGCGA	30	2.1635133E-6	44.999996	30
GTAATCG	25	3.8882506E-5	44.999996	1
ACGATCA	25	3.8882506E-5	44.999996	40
CCCGTAA	25	3.8882506E-5	44.999996	41
AATTGCG	30	2.1635133E-6	44.999996	1
>>END_MODULE