##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551879_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	505903
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.670407172916548	28.0	25.0	31.0	16.0	33.0
2	27.84321105033969	31.0	25.0	33.0	16.0	34.0
3	27.663055961320648	31.0	25.0	33.0	16.0	34.0
4	31.66974103731348	35.0	28.0	35.0	19.0	37.0
5	33.31430728815603	35.0	32.0	35.0	28.0	37.0
6	33.15131359173596	35.0	32.0	35.0	28.0	37.0
7	33.99603481299775	35.0	33.0	37.0	31.0	37.0
8	34.044986884837606	35.0	35.0	37.0	31.0	37.0
9	36.30145304534664	39.0	35.0	39.0	32.0	39.0
10	35.516587171849146	37.0	34.0	39.0	31.0	39.0
11	36.159771734897795	37.0	35.0	39.0	32.0	39.0
12	36.22501151406495	37.0	35.0	39.0	32.0	39.0
13	36.37060068827424	37.0	35.0	39.0	32.0	39.0
14	37.35182040826008	39.0	36.0	40.0	32.0	41.0
15	37.488664823098496	39.0	36.0	40.0	32.0	41.0
16	37.08613311247413	39.0	36.0	40.0	32.0	41.0
17	37.10251372298642	38.0	36.0	40.0	32.0	41.0
18	37.00085391863658	38.0	36.0	40.0	32.0	41.0
19	36.6378021083093	38.0	35.0	40.0	31.0	41.0
20	36.81744326481559	38.0	35.0	40.0	32.0	41.0
21	37.06571417840969	39.0	35.0	40.0	32.0	41.0
22	37.421266527377774	39.0	36.0	40.0	33.0	41.0
23	37.717483391084855	39.0	36.0	40.0	33.0	41.0
24	37.75546498044091	39.0	36.0	41.0	33.0	41.0
25	36.841787852612065	39.0	35.0	40.0	31.0	41.0
26	37.12813721207425	39.0	35.0	40.0	32.0	41.0
27	37.43425122997887	39.0	36.0	40.0	33.0	41.0
28	37.45178423531784	39.0	36.0	41.0	33.0	41.0
29	37.561765793047286	40.0	36.0	41.0	33.0	41.0
30	37.195855727283686	39.0	36.0	40.0	32.0	41.0
31	37.33448506927217	39.0	36.0	40.0	33.0	41.0
32	37.213691162139774	39.0	36.0	40.0	33.0	41.0
33	37.19012735642999	40.0	36.0	41.0	32.0	41.0
34	37.10094425215901	40.0	36.0	41.0	32.0	41.0
35	37.04016580253527	40.0	36.0	41.0	32.0	41.0
36	37.031098847012174	40.0	36.0	41.0	32.0	41.0
37	37.01823076755821	40.0	36.0	41.0	32.0	41.0
38	36.94640276891025	40.0	36.0	41.0	31.0	41.0
39	36.84334546345841	40.0	36.0	41.0	31.0	41.0
40	36.71082796504468	39.0	35.0	41.0	31.0	41.0
41	36.475241301198054	39.0	35.0	41.0	30.0	41.0
42	36.68918152294017	39.0	35.0	41.0	31.0	41.0
43	36.69264266074722	40.0	35.0	41.0	31.0	41.0
44	36.80445263222396	40.0	36.0	41.0	31.0	41.0
45	36.79552799647363	40.0	36.0	41.0	32.0	41.0
46	36.6396858686349	39.0	35.0	41.0	31.0	41.0
47	36.477998746795336	39.0	35.0	41.0	31.0	41.0
48	36.552764067420036	39.0	35.0	41.0	31.0	41.0
49	36.568405405779366	39.0	35.0	41.0	31.0	41.0
50	36.46543309685849	39.0	35.0	41.0	31.0	41.0
51	35.50594086218109	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	14.0
10	9.0
11	7.0
12	19.0
13	19.0
14	32.0
15	35.0
16	47.0
17	86.0
18	179.0
19	342.0
20	676.0
21	1261.0
22	1939.0
23	2586.0
24	3528.0
25	4824.0
26	5428.0
27	5612.0
28	5640.0
29	6278.0
30	8278.0
31	11000.0
32	15660.0
33	23610.0
34	32916.0
35	41178.0
36	62396.0
37	102366.0
38	129045.0
39	40887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.028011298608625	18.33987938399258	21.056408046601817	17.57570127079697
2	30.691061329938744	28.662213902665133	22.96705099594191	17.679673771454212
3	28.346540740023286	27.08799908282813	27.3787662852365	17.186693891912086
4	26.204035160890527	22.855171841242296	32.92152843529293	18.01926456257425
5	28.535707437987124	26.254242414059615	25.604513118127386	19.605537029825875
6	23.04512920461037	36.17827923534749	25.986404508374132	14.790187051668008
7	71.41863163491816	5.464881607739033	17.830690863663587	5.285795893679223
8	70.92486108997178	10.588195760847436	12.421551166923303	6.065391982257468
9	64.91145535804294	8.66905315841179	13.381814300369834	13.03767718317543
10	34.54081118317148	30.960282900081637	20.998689472092476	13.50021644465441
11	27.389440268193706	24.05421592676857	29.826468710404956	18.729875094632767
12	24.69860823122219	20.719189251694495	32.68096848605365	21.901234031029666
13	23.503122140014984	22.22936017378826	35.672648709337565	18.5948689768592
14	18.984469354797266	28.363540046214393	30.674062023747634	21.977928575240707
15	17.49090240619249	26.01486846292669	36.996420262382316	19.497808868498506
16	19.569166421230946	26.66005143278455	30.73158293190592	23.039199214078586
17	19.4997855320091	26.952992965054566	32.09429475610937	21.45292674682696
18	19.026572287572915	27.207784891570125	31.798388228573458	21.9672545922835
19	20.14872416253709	27.428182873001344	30.130874891036424	22.292218073425143
20	21.846085119084094	27.447751841756222	33.67740456174405	17.028758477415632
21	21.808528512382807	29.179704409738626	31.23266713184148	17.779099946037086
22	19.02083996339219	24.04176294665183	32.75548870040304	24.181908389552937
23	19.71682318547231	27.627825887571333	32.1978719240645	20.45747900289186
24	21.21216913123662	25.463577009822043	30.556252878516236	22.7680009804251
25	18.592496980646487	31.50979535602675	28.363935378916512	21.53377228441025
26	18.491687141606196	27.861862847225655	31.345139285594275	22.301310725573874
27	21.80477285171268	27.696613777740005	29.745425506470607	20.75318786407671
28	17.30351470538819	28.05201787694479	32.51512641751482	22.129341000152202
29	19.612455352112953	27.459414156468732	30.621285108014778	22.306845383403537
30	18.385144978385185	27.969591008553024	32.57086832851357	21.074395684548225
31	20.504325928092936	26.832021158206214	27.21727287642097	25.446380037279877
32	21.074593350899285	30.85235707240321	27.142159663018404	20.930889913679103
33	19.852619968650117	26.37580721996114	26.94231898209736	26.82925382929139
34	22.47901277517627	27.34180267758839	27.104207723615005	23.07497682362034
35	19.11908013986871	26.37995821333339	28.37124903390571	26.1297126128922
36	18.951459074170344	30.257183689363377	27.16667029054977	23.62468694591651
37	18.450572540585846	27.764018003451252	32.088760098279714	21.696649357683192
38	19.100301836518067	26.849811129801566	25.93362759264128	28.116259441039094
39	23.128346738406375	28.09056281540137	27.044512485595064	21.736577960597188
40	20.416759734573624	24.605309713522157	29.466122952423685	25.511807599480534
41	20.462815994370462	28.71736281461071	27.139392334103572	23.68042885691526
42	18.809336967758643	26.328762628409002	30.491813648070874	24.37008675576148
43	21.069651692122797	28.066645186923182	27.22181920249534	23.641883918458678
44	20.93662223785983	25.152450173254557	29.349302138947586	24.56162544993803
45	20.055425644837054	24.13565446340504	27.950022039798146	27.858897851959764
46	22.623506877800686	27.888547804618675	27.018618193606287	22.46932712397436
47	17.976370964394363	25.209971081412842	34.03221566189566	22.78144229229714
48	19.94295349108426	26.07555203270192	28.6214946343469	25.35999984186692
49	19.535958474252972	23.890350521740334	32.52817239668474	24.045518607321956
50	20.257835988321872	24.273823242795554	29.420659691680022	26.047681077202544
51	19.33078080185332	23.665208547883683	27.92412774780936	29.07988290245363
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	109.0
1	357.0
2	605.0
3	3646.5
4	6688.0
5	4538.5
6	2389.0
7	2335.5
8	2282.0
9	2149.0
10	2016.0
11	1982.5
12	1949.0
13	1970.5
14	1992.0
15	1896.0
16	1800.0
17	1755.5
18	1711.0
19	1764.0
20	1817.0
21	1961.5
22	2106.0
23	2323.5
24	2541.0
25	3046.0
26	4282.0
27	5013.0
28	6085.5
29	7158.0
30	8657.0
31	10156.0
32	11304.0
33	12452.0
34	13338.0
35	14224.0
36	15752.0
37	17280.0
38	19195.0
39	21110.0
40	22794.5
41	24479.0
42	25952.5
43	27426.0
44	29990.5
45	32555.0
46	52424.5
47	72294.0
48	61084.0
49	49874.0
50	47860.0
51	45846.0
52	39353.5
53	32861.0
54	28446.0
55	24031.0
56	21219.5
57	18408.0
58	16868.5
59	15329.0
60	14328.5
61	13328.0
62	11851.0
63	10374.0
64	8723.5
65	7073.0
66	5622.5
67	4172.0
68	3471.0
69	2770.0
70	2393.5
71	2017.0
72	1717.0
73	1417.0
74	1196.5
75	867.0
76	758.0
77	613.5
78	469.0
79	368.0
80	267.0
81	203.0
82	139.0
83	95.5
84	52.0
85	36.0
86	20.0
87	16.5
88	13.0
89	8.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	505903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.08546542869993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.6444463514617	26.189351419086567
2	10.087693936409778	7.078628737224224
3	3.5557878127564995	3.742694111287822
4	1.8507428240964119	2.5973669348899655
5	1.2302655646900562	2.1582219969026486
6	0.9118274025868461	1.9195133286241233
7	0.7397417572493757	1.8167928695097098
8	0.6585603252540736	1.8484716419532148
9	0.5576611826229105	1.7609221929459642
>10	5.649969151057596	38.25826935671171
>50	0.07308088122051938	1.6746005010391696
>100	0.03229155216720619	2.1170528114620977
>500	0.002266073836295171	0.5764203505060637
>1k	0.00396562921351655	3.1893934290414814
>5k	0.0016995553772213784	5.0723003188152544
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTC	8988	1.7766251633218224	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCG	8303	1.6412237128461384	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGC	8228	1.626398736516684	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4032	0.7969907274714718	No Hit
GCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTGC	3142	0.6210676750286123	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCACGCAT	2353	0.4651089240427513	No Hit
GAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCT	2089	0.41292500736307153	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCT	1848	0.3652874167577579	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTC	1341	0.26507057677064577	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCACGCATCGTA	1241	0.24530394166470648	No Hit
CCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTGC	882	0.17434172163438447	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCC	811	0.16030741070916757	No Hit
CTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTGCT	705	0.13935477749687195	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1423197727627628	0.0
2	0.0	0.0	0.0	1.1118732247090846	0.0
3	0.0	0.0	0.0	1.3923617768623628	0.0
4	0.0	0.0	0.0	2.15179589763255	0.0
5	0.0	0.0	0.0	4.9054858342409515	0.0
6	0.0	0.0	0.0	5.627363348309854	0.0
7	0.0	0.0	0.0	6.580115160416127	0.0
8	0.0	0.0	0.0	7.714127016443864	0.0
9	0.0	0.0	0.0	8.094832408584255	0.0
10	0.0	0.0	0.0	10.6127063883788	0.0
11	0.0	0.0	0.0	11.921059966040922	0.0
12	0.0	0.0	0.0	14.614461665576208	0.0
13	0.0	0.0	0.0	15.14756781438339	0.0
14	0.0	0.0	0.0	15.391092758888561	0.0
15	0.0	0.0	0.0	15.932105561738119	0.0
16	0.0	0.0	0.0	16.629670114626716	0.0
17	0.0	0.0	0.0	17.398196887545637	0.0
18	0.0	0.0	0.0	18.18372296665566	0.0
19	0.0	0.0	0.0	19.19399568692022	0.0
20	0.0	0.0	0.0	19.85163163689482	0.0
21	0.0	0.0	0.0	20.478036303402035	0.0
22	0.0	0.0	0.0	21.242214416597648	0.0
23	0.0	0.0	0.0	21.928116654773742	0.0
24	0.0	0.0	0.0	22.48869842637818	0.0
25	0.0	0.0	0.0	22.970608990260978	0.0
26	0.0	0.0	0.0	23.442240903888692	0.0
27	0.0	0.0	0.0	23.938778777749885	0.0
28	0.0	0.0	0.0	24.410213025026536	0.0
29	1.976663510593928E-4	0.0	0.0	24.93284285722757	0.0
30	1.976663510593928E-4	0.0	0.0	25.48670397289599	0.0
31	1.976663510593928E-4	0.0	0.0	26.016845126437282	0.0
32	1.976663510593928E-4	0.0	0.0	26.524254649606743	0.0
33	1.976663510593928E-4	0.0	0.0	27.021583188872174	0.0
34	1.976663510593928E-4	0.0	0.0	27.49202910439353	0.0
35	1.976663510593928E-4	0.0	0.0	28.008531279711722	0.0
36	1.976663510593928E-4	0.0	0.0	28.53135877826382	0.0
37	1.976663510593928E-4	0.0	0.0	29.059127935592397	0.0
38	1.976663510593928E-4	0.0	0.0	29.57286278199576	0.0
39	1.976663510593928E-4	0.0	0.0	30.07928397340992	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	35	1.2101191E-7	45.000004	2
CTATGCG	20	7.029538E-4	45.0	1
CGGTTAT	20	7.029538E-4	45.0	42
CTGCCGG	20	7.029538E-4	45.0	2
CGAATAT	40	6.8012014E-9	45.0	14
CCGGTTA	20	7.029538E-4	45.0	41
ACGTAGG	20	7.029538E-4	45.0	2
TGTAACG	20	7.029538E-4	45.0	1
AGCCGTT	30	2.163084E-6	44.999996	45
ATCGTAG	30	2.163084E-6	44.999996	1
CGTTTTT	2330	0.0	42.972107	1
TACGGCT	855	0.0	42.894737	7
CGGCGAT	80	0.0	42.1875	31
TCGAATG	80	0.0	42.1875	44
TTAGCGG	70	0.0	41.785717	2
TTGTGCG	60	3.6379788E-12	41.249996	1
ACCACCG	560	0.0	40.982143	14
ACGGCTG	895	0.0	40.977654	8
CATAAGG	55	6.002665E-11	40.909092	2
CGCGAGG	50	1.0786607E-9	40.5	2
>>END_MODULE