SRR3551874_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551874_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434802
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	13630	3.1347601896955393	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	11574	2.6619012792029473	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	9732	2.2382601735962577	No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	4194	0.964576979866698	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3693	0.8493521188954972	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCGTGTTT	3582	0.8238232574827163	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	2502	0.575434335628631	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCT	2406	0.5533553203527122	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTA	1879	0.43215072607761695	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTC	1855	0.4266309722586373	No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1568	0.36062391617333867	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	1000	0.2299897424574864	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGCGTGTT	811	0.18652168113302148	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTT	675	0.1552430761588033	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCC	580	0.13339405062534213	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGT	572	0.13155413268568222	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATG	525	0.12074461479018035	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTAT	463	0.1064852507578162	No Hit
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	456	0.10487532256061378	TruSeq Adapter, Index 14 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	45	3.8380676E-10	45.0	13
CTCCGGA	20	7.0284447E-4	45.0	18
CCGATCG	50	2.1827873E-11	45.0	40
ATATGCG	20	7.0284447E-4	45.0	1
GACCATA	20	7.0284447E-4	45.0	9
AACGGGT	40	6.7975634E-9	45.0	4
TACGATC	20	7.0284447E-4	45.0	45
CGAATAC	25	3.886791E-5	45.0	45
TCCCGTT	25	3.886791E-5	45.0	35
TCTCCCG	25	3.886791E-5	45.0	33
CTCGAGT	20	7.0284447E-4	45.0	20
TTACGCT	20	7.0284447E-4	45.0	28
TTACGCG	20	7.0284447E-4	45.0	1
ATAACGG	25	3.886791E-5	45.0	2
CGCAATA	20	7.0284447E-4	45.0	4
TTCGATA	20	7.0284447E-4	45.0	19
TGTCGCG	20	7.0284447E-4	45.0	1
TCGAATA	25	3.886791E-5	45.0	44
TCGGTCG	20	7.0284447E-4	45.0	2
CCTCTAG	20	7.0284447E-4	45.0	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content