##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551874_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434802
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.66513033518705	30.0	25.0	31.0	16.0	33.0
2	27.865844683327122	30.0	25.0	33.0	16.0	33.0
3	27.62098610401976	30.0	25.0	33.0	16.0	34.0
4	31.80453631768023	35.0	28.0	35.0	19.0	37.0
5	33.33280205702825	35.0	32.0	35.0	28.0	37.0
6	33.242544882498244	35.0	32.0	35.0	28.0	37.0
7	33.94578681790792	35.0	33.0	36.0	31.0	37.0
8	33.66470255426608	35.0	33.0	37.0	28.0	37.0
9	35.89012930023321	39.0	35.0	39.0	30.0	39.0
10	35.366957833680615	37.0	34.0	39.0	31.0	39.0
11	36.1406525268973	37.0	35.0	39.0	32.0	39.0
12	36.19565457380601	37.0	35.0	39.0	32.0	39.0
13	36.241174143633195	37.0	35.0	39.0	32.0	39.0
14	37.2607347712292	39.0	36.0	40.0	32.0	41.0
15	37.34769389285238	39.0	36.0	40.0	32.0	41.0
16	36.967362155647855	38.0	35.0	40.0	32.0	41.0
17	36.90991991757168	38.0	36.0	40.0	32.0	41.0
18	36.85806873013463	38.0	36.0	40.0	32.0	41.0
19	36.525618557412336	38.0	34.0	40.0	31.0	41.0
20	36.5465223251043	38.0	35.0	40.0	31.0	41.0
21	36.979310122768524	39.0	35.0	40.0	32.0	41.0
22	37.26545876053928	39.0	36.0	40.0	32.0	41.0
23	37.57113352744467	39.0	36.0	40.0	33.0	41.0
24	37.55183738805249	39.0	36.0	41.0	32.0	41.0
25	36.85413590553861	39.0	35.0	40.0	31.0	41.0
26	37.043840644707245	39.0	35.0	40.0	32.0	41.0
27	37.24755175919154	39.0	36.0	40.0	32.0	41.0
28	37.194040045813956	39.0	36.0	40.0	32.0	41.0
29	37.312829747793245	39.0	36.0	41.0	32.0	41.0
30	36.88000745166766	39.0	35.0	40.0	31.0	41.0
31	37.23574868560862	39.0	36.0	40.0	32.0	41.0
32	36.98778984457293	39.0	36.0	40.0	31.0	41.0
33	36.814658166245785	39.0	35.0	41.0	30.0	41.0
34	36.794814651266556	40.0	36.0	41.0	31.0	41.0
35	36.70378931099673	40.0	36.0	41.0	30.0	41.0
36	36.58934871504731	40.0	35.0	41.0	30.0	41.0
37	36.727383958675446	40.0	36.0	41.0	31.0	41.0
38	36.471614665985896	39.0	35.0	41.0	30.0	41.0
39	36.46192289823874	39.0	35.0	41.0	30.0	41.0
40	36.115650341994744	39.0	35.0	40.0	29.0	41.0
41	36.05260325389488	39.0	35.0	40.0	28.0	41.0
42	36.27835428539887	39.0	35.0	41.0	30.0	41.0
43	36.348947336948775	39.0	35.0	41.0	30.0	41.0
44	36.575802319216564	39.0	35.0	41.0	31.0	41.0
45	36.57036076191002	39.0	35.0	41.0	31.0	41.0
46	36.45701721703212	39.0	35.0	40.0	31.0	41.0
47	36.22412960381967	39.0	35.0	41.0	30.0	41.0
48	36.305663267418275	39.0	35.0	41.0	30.0	41.0
49	36.34855865428402	39.0	35.0	41.0	30.0	41.0
50	36.190562600908	39.0	35.0	41.0	30.0	41.0
51	35.2293733699477	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	7.0
10	16.0
11	10.0
12	11.0
13	18.0
14	9.0
15	24.0
16	40.0
17	76.0
18	152.0
19	414.0
20	851.0
21	1391.0
22	1833.0
23	2483.0
24	3247.0
25	4473.0
26	5113.0
27	5333.0
28	5424.0
29	6376.0
30	7958.0
31	10601.0
32	14671.0
33	21366.0
34	29213.0
35	36329.0
36	53261.0
37	87163.0
38	105594.0
39	31336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.940207266755905	16.85410830676952	18.691496359262377	15.514188067212203
2	29.437077106361052	32.82643594095703	20.987483958215464	16.749002994466448
3	27.952493318797984	32.02124185261337	25.114419896872597	14.911844931716045
4	25.432035731206387	20.56798266797301	36.88276502867972	17.117216572140883
5	32.595986219014634	23.826017359625762	24.40421157216388	19.173784849195727
6	21.804407523424455	38.19945630424883	25.88925533921187	14.106880833114843
7	66.1655190178518	5.876237919788777	22.539454740318583	5.418788322040837
8	63.04800805884058	15.374814283282964	14.315021550038868	7.2621561078375905
9	57.59403130620374	8.747659854370495	14.971182285270077	18.687126554155682
10	33.74593493130206	27.672135822742305	23.149387537315835	15.432541708639794
11	30.403494004167413	22.933657158890714	28.31794701956293	18.344901817378943
12	26.23699982980759	19.289929669136757	31.079433857249967	23.39363664380569
13	24.336134608396463	21.137437270297745	36.1141393093868	18.41228881191899
14	18.119741859513066	29.57116112621377	28.7947157556773	23.51438125859587
15	15.52361764665296	25.144548553134534	39.14126429961224	20.190569500600272
16	18.26210551009425	25.682264571000136	29.370610070790843	26.68501984811477
17	18.329492504634292	27.00907539523737	33.115533047226094	21.54589905290224
18	17.151255054024592	26.40650226999876	33.1371520830171	23.305090592959555
19	18.791081917746467	27.421447003463644	29.310122768524522	24.477348310265363
20	21.727360959701198	26.382583336783178	35.74362583428779	16.146429869227834
21	21.605696385941187	30.117846744035216	30.344156650613385	17.932300219410212
22	18.13653111071246	23.063371373636734	32.94442067883772	25.85567683681308
23	20.002897870754964	28.58496511055607	31.791482099898342	19.620654918790624
24	21.691022580392914	24.42537062846997	29.365090316971866	24.518516474165253
25	17.663442210477413	32.71880074148693	27.628437771675387	21.989319276360277
26	18.266015335716027	24.82072299575439	33.966495094318795	22.946766574210788
27	24.04657752264249	26.219060629895907	28.275398917208292	21.45896293025331
28	16.551441805695465	26.787595273250815	33.585862070551656	23.075100850502068
29	23.14708763989126	22.193320177920064	32.88278342785912	21.776808754329558
30	21.088909434639216	27.934554118886297	31.077133959825392	19.899402486649095
31	22.391571335918417	23.174686408986158	26.666850658460632	27.766891596634792
32	26.879131190748893	26.609123233103805	28.349455614279602	18.1622899618677
33	21.461032837935427	22.233568382850123	31.20983804122336	25.095560737991086
34	24.53162588948533	22.884899333489727	31.93660562738902	20.646869149635926
35	24.176981706615884	22.180670742084903	32.0357312063882	21.606616344911018
36	20.24300716188058	23.930202712959	33.113003160059066	22.713786965101356
37	25.731482375886035	22.912268112842167	31.88669785327574	19.469551657996053
38	19.851794609960397	23.394786592517974	30.771477592099394	25.98194120542224
39	28.543566956913725	22.014848137773054	29.79609109433719	19.64549381097603
40	20.618580411313655	21.59925667315238	37.176001950313015	20.60616096522095
41	21.094429188458193	24.63121144796942	31.917746468507502	22.35661289506488
42	19.919181604500437	22.921467702540465	36.43773487702448	20.72161581593461
43	22.971835456138656	22.42147000243789	31.488585609081838	23.11810893234162
44	23.90306392334902	21.460572858450515	30.792866638147938	23.843496580052527
45	20.50565544776703	20.60294110882655	30.7855069663893	28.105896477017122
46	25.815658621625477	24.94169760028703	28.950418811321015	20.292224966766483
47	18.57167170344203	22.80049309800783	38.60722811762595	20.02060708092419
48	22.679058513990277	22.850170882378645	29.27516432767099	25.195606275960092
49	20.607310913933237	20.13491198292556	36.496382261351144	22.761394841790057
50	20.827181107722595	20.329483305044597	33.12703253434896	25.716303052883845
51	20.695626975036912	20.12111259837811	30.84300440200367	28.340256024581308
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	178.0
1	350.5
2	523.0
3	3290.0
4	6057.0
5	4087.5
6	2118.0
7	2063.0
8	2008.0
9	1850.5
10	1693.0
11	1621.0
12	1549.0
13	1487.5
14	1426.0
15	1338.0
16	1250.0
17	1286.0
18	1322.0
19	1360.5
20	1399.0
21	1474.5
22	1550.0
23	1800.5
24	2051.0
25	2558.5
26	3513.0
27	3960.0
28	4804.5
29	5649.0
30	6687.0
31	7725.0
32	8311.0
33	8897.0
34	10296.0
35	11695.0
36	12016.0
37	12337.0
38	13730.5
39	15124.0
40	16886.5
41	18649.0
42	20270.5
43	21892.0
44	24060.0
45	26228.0
46	51540.5
47	76853.0
48	59737.5
49	42622.0
50	41377.5
51	40133.0
52	33468.0
53	26803.0
54	23859.5
55	20916.0
56	19248.0
57	17580.0
58	16201.5
59	14823.0
60	13700.0
61	12577.0
62	11479.5
63	10382.0
64	7992.0
65	5602.0
66	4407.0
67	3212.0
68	2702.5
69	2193.0
70	1645.0
71	1097.0
72	922.0
73	747.0
74	586.0
75	338.5
76	252.0
77	207.5
78	163.0
79	107.0
80	51.0
81	32.5
82	14.0
83	10.0
84	6.0
85	4.5
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434802.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.97912369791436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.55787004754487	24.58853218951006
2	10.111110270539962	6.669111126707794
3	3.5994469884121445	3.561198224247883
4	1.7922971983851603	2.364335640358782
5	1.1736789621185304	1.9353451836673377
6	0.8906708434919076	1.7624126352987206
7	0.7110685843810826	1.6415293161403202
8	0.6076561111380471	1.60319728440122
9	0.521408084533249	1.5476023545223143
>10	5.906736548603608	36.54365897889559
>50	0.08117233802608328	1.747891532349363
>100	0.03498807673538073	2.0091290837910685
>500	0.004198569208245687	0.9607172496924211
>1k	0.005598092277660917	5.002976040375209
>5k	6.997615347076146E-4	2.2459044616878794
>10k+	0.0013995230694152292	5.816458698354019
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	13630	3.1347601896955393	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	11574	2.6619012792029473	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	9732	2.2382601735962577	No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	4194	0.964576979866698	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3693	0.8493521188954972	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCGTGTTT	3582	0.8238232574827163	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	2502	0.575434335628631	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCT	2406	0.5533553203527122	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTA	1879	0.43215072607761695	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTC	1855	0.4266309722586373	No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1568	0.36062391617333867	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	1000	0.2299897424574864	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGCGTGTT	811	0.18652168113302148	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTT	675	0.1552430761588033	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCC	580	0.13339405062534213	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGT	572	0.13155413268568222	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATG	525	0.12074461479018035	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTAT	463	0.1064852507578162	No Hit
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	456	0.10487532256061378	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2382693731859559	0.0
2	0.0	0.0	0.0	1.7985197860175437	0.0
3	0.0	0.0	0.0	2.1253352100496317	0.0
4	0.0	0.0	0.0	3.0972718616749693	0.0
5	0.0	0.0	0.0	6.853464335490637	0.0
6	0.0	0.0	0.0	7.614040413797545	0.0
7	0.0	0.0	0.0	8.735010418535333	0.0
8	0.0	0.0	0.0	9.877599459064125	0.0
9	0.0	0.0	0.0	10.198895129277235	0.0
10	0.0	0.0	0.0	14.198186760870465	0.0
11	0.0	0.0	0.0	15.334106098867991	0.0
12	0.0	0.0	0.0	18.560862185546526	0.0
13	0.0	0.0	0.0	19.0716694035446	0.0
14	0.0	0.0	0.0	19.380545627665008	0.0
15	0.0	0.0	0.0	20.162970731505375	0.0
16	0.0	0.0	0.0	20.886748451019084	0.0
17	0.0	0.0	0.0	21.67492329842089	0.0
18	0.0	0.0	0.0	22.358222823262082	0.0
19	0.0	0.0	0.0	23.714932314018796	0.0
20	0.0	0.0	0.0	24.41088127469515	0.0
21	0.0	0.0	0.0	25.026103835768925	0.0
22	0.0	0.0	0.0	25.782080119226684	0.0
23	0.0	0.0	0.0	26.426971357077473	0.0
24	0.0	0.0	0.0	26.966527292882738	0.0
25	0.0	0.0	0.0	27.433636459813894	0.0
26	0.0	0.0	0.0	27.899365688290303	0.0
27	0.0	0.0	0.0	28.404193172984485	0.0
28	0.0	0.0	0.0	28.8347339708649	0.0
29	0.0	0.0	0.0	29.28459390711174	0.0
30	0.0	0.0	0.0	29.78643152515398	0.0
31	0.0	0.0	0.0	30.252620733115304	0.0
32	0.0	0.0	0.0	30.71214023854536	0.0
33	0.0	0.0	0.0	31.156250431230767	0.0
34	0.0	0.0	0.0	31.622899618677007	0.0
35	0.0	0.0	0.0	32.08471902153164	0.0
36	0.0	0.0	0.0	32.5734472242538	0.0
37	0.0	0.0	0.0	33.05895557058155	0.0
38	0.0	0.0	0.0	33.53089452210431	0.0
39	0.0	0.0	0.0	34.005593350536564	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	45	3.8380676E-10	45.0	13
CTCCGGA	20	7.0284447E-4	45.0	18
CCGATCG	50	2.1827873E-11	45.0	40
ATATGCG	20	7.0284447E-4	45.0	1
GACCATA	20	7.0284447E-4	45.0	9
AACGGGT	40	6.7975634E-9	45.0	4
TACGATC	20	7.0284447E-4	45.0	45
CGAATAC	25	3.886791E-5	45.0	45
TCCCGTT	25	3.886791E-5	45.0	35
TCTCCCG	25	3.886791E-5	45.0	33
CTCGAGT	20	7.0284447E-4	45.0	20
TTACGCT	20	7.0284447E-4	45.0	28
TTACGCG	20	7.0284447E-4	45.0	1
ATAACGG	25	3.886791E-5	45.0	2
CGCAATA	20	7.0284447E-4	45.0	4
TTCGATA	20	7.0284447E-4	45.0	19
TGTCGCG	20	7.0284447E-4	45.0	1
TCGAATA	25	3.886791E-5	45.0	44
TCGGTCG	20	7.0284447E-4	45.0	2
CCTCTAG	20	7.0284447E-4	45.0	45
>>END_MODULE