FastQCFastQC Report
Sat 18 Jun 2016
SRR3551873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314461
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG91962.924368999653375No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC84372.6830036157106925No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC81202.582196202390757No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29710.9447912459732686No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC25090.7978731861820703No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTACCGT21910.696747768403713No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCT16630.5288414143566292No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT15960.5075351156423213No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTA13530.43026003224565207No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTC12480.3968695641112888No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAGTACCG4950.157412206919141No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATG4760.1513701222091134No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC4690.14914409100015583No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGT4090.13006382349480541No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT4050.12879180566111537No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC3560.11320958719841251No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT3430.10907552923891993No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3280.10430546236258231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCC302.1604537E-645.00000434
CTACGAA650.045.00000411
TACGAAT650.045.00000412
TGCTATA302.1604537E-645.00000432
AGCGTTC207.025463E-445.07
ATTAGTC253.8843227E-545.031
GCGACAC207.025463E-445.033
CTCAACG207.025463E-445.01
GTCGAAT950.045.043
GTACACG207.025463E-445.01
CGGCCTT253.8843227E-545.044
GCGAACC351.2081728E-745.033
TAGCGCG253.8843227E-545.01
ACTCTCG207.025463E-445.033
TTGTCCG207.025463E-445.025
TTCGTAC207.025463E-445.033
CGATTGG253.8843227E-545.02
ACGGGCC406.7884685E-945.05
CCGTCGA950.045.041
CCCACGC207.025463E-445.039