##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551873_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	314461
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.64549816988434	30.0	25.0	31.0	16.0	33.0
2	27.828605137044022	30.0	25.0	33.0	16.0	33.0
3	27.596916628771137	30.0	25.0	33.0	16.0	34.0
4	31.745189387555214	35.0	28.0	35.0	19.0	37.0
5	33.317702354187006	35.0	32.0	35.0	28.0	37.0
6	33.21499963429487	35.0	32.0	35.0	28.0	37.0
7	33.95228025096912	35.0	33.0	36.0	31.0	37.0
8	33.66260680974747	35.0	33.0	37.0	28.0	37.0
9	35.9588152425897	39.0	35.0	39.0	30.0	39.0
10	35.36091598004204	37.0	34.0	39.0	31.0	39.0
11	36.1131110058163	37.0	35.0	39.0	32.0	39.0
12	36.14286668299089	37.0	35.0	39.0	32.0	39.0
13	36.119353433335135	37.0	35.0	39.0	32.0	39.0
14	37.16134592206983	39.0	36.0	40.0	32.0	41.0
15	37.23060411306967	39.0	36.0	40.0	32.0	41.0
16	36.91510552977953	38.0	35.0	40.0	32.0	41.0
17	36.81687077252823	38.0	35.0	40.0	31.0	41.0
18	36.81502634666938	38.0	35.0	40.0	32.0	41.0
19	36.469651244510445	38.0	34.0	40.0	31.0	41.0
20	36.40775485672309	38.0	35.0	40.0	30.0	41.0
21	36.90494210728834	39.0	35.0	40.0	32.0	41.0
22	37.156391412607604	39.0	35.0	40.0	32.0	41.0
23	37.43459443301395	39.0	35.0	40.0	32.0	41.0
24	37.47888927402762	39.0	35.0	41.0	32.0	41.0
25	36.7092485236643	38.0	35.0	40.0	31.0	41.0
26	36.897771106750916	39.0	35.0	40.0	31.0	41.0
27	37.0593269117633	39.0	35.0	40.0	32.0	41.0
28	37.0144533026353	39.0	35.0	40.0	31.0	41.0
29	37.05090297365969	39.0	35.0	41.0	31.0	41.0
30	36.79439739745151	39.0	35.0	40.0	31.0	41.0
31	36.94481032624078	39.0	35.0	40.0	31.0	41.0
32	36.706322882646816	39.0	35.0	40.0	31.0	41.0
33	36.6301862552113	39.0	35.0	41.0	30.0	41.0
34	36.56906261825791	39.0	35.0	41.0	30.0	41.0
35	36.420182470958245	39.0	35.0	41.0	30.0	41.0
36	36.42125414598313	39.0	35.0	41.0	30.0	41.0
37	36.299795523133234	39.0	35.0	41.0	30.0	41.0
38	36.21968383996744	39.0	35.0	41.0	30.0	41.0
39	36.03970285663405	39.0	35.0	41.0	29.0	41.0
40	35.945322313418835	39.0	35.0	40.0	28.0	41.0
41	35.84645790733986	39.0	35.0	40.0	26.0	41.0
42	36.067089400593396	39.0	35.0	40.0	29.0	41.0
43	36.04842571892858	39.0	35.0	41.0	29.0	41.0
44	36.290716495845274	39.0	35.0	41.0	30.0	41.0
45	36.32841592439126	39.0	35.0	41.0	30.0	41.0
46	36.13991242157215	39.0	35.0	40.0	29.0	41.0
47	35.92846807712244	39.0	35.0	41.0	27.0	41.0
48	36.01638676974251	39.0	35.0	40.0	29.0	41.0
49	36.06922957059858	39.0	35.0	40.0	29.0	41.0
50	35.90742572210863	39.0	35.0	40.0	28.0	41.0
51	35.06600818543476	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	16.0
11	7.0
12	8.0
13	8.0
14	15.0
15	15.0
16	35.0
17	74.0
18	115.0
19	297.0
20	736.0
21	1302.0
22	1626.0
23	2167.0
24	2779.0
25	3563.0
26	4089.0
27	4127.0
28	4225.0
29	4716.0
30	5946.0
31	7799.0
32	11020.0
33	16229.0
34	21670.0
35	26033.0
36	38282.0
37	61670.0
38	73403.0
39	22481.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.85502494744976	16.365781448255902	19.433888463116254	15.345305141178079
2	30.53001803085279	33.12716044278941	19.68479398081161	16.65802754554619
3	27.246303993181986	32.342007434944236	24.683188058296576	15.7285005135772
4	24.9547638657894	20.471537010948893	37.62247146704997	16.95122765621174
5	32.55125436858625	24.222717602500786	23.93905762558791	19.286970403325054
6	22.127386225954886	38.75393133011725	25.835954220078168	13.282728223849698
7	66.37611659315463	5.626452882869418	22.884554841458876	5.1128756825170685
8	63.83780500602618	15.995624258652105	13.917783127319444	6.248787608002264
9	57.77854805524374	9.147080242064993	14.427862278629147	18.646509424062124
10	35.3481035804122	27.32008102753601	22.51312563402139	14.818689758030407
11	31.67419807225697	22.51566966968877	27.527737938885906	18.282394319168354
12	25.74500494496933	19.238951730103256	30.72749879953317	24.288544525394247
13	24.764915204111162	21.124082159631875	36.712660711503176	17.398341924753787
14	19.503531439510784	29.06242745523292	29.003278625966335	22.43076247928996
15	16.13650023373328	24.93250355369982	38.763153459411505	20.167842753155398
16	18.186356972724756	25.67281793290742	29.12062227112424	27.020202823243583
17	18.59626471963137	27.50420560896264	32.953212004032295	20.946317667373695
18	18.273490194332524	25.514133708154592	32.529630065413514	23.68274603209937
19	19.42244030261304	27.051685264627412	29.19885136789618	24.327023064863372
20	21.75086894718264	27.318809009702317	34.563586581483875	16.366735461631173
21	21.14729648509672	31.16189288973831	30.294694731620137	17.396115893544827
22	18.416910205081074	22.794559579725306	34.162900963871515	24.625629251322103
23	20.617501057364823	28.301760790686288	32.331831292274714	18.74890685967417
24	20.97557407754857	25.400606116497755	29.321919093305688	24.301900712647992
25	17.40024995150432	34.35561166567555	27.950683868587838	20.29345451423229
26	17.87980067480546	26.350803438264204	32.5350361412067	23.234359745723633
27	22.71124241161861	28.2934926747673	27.864186655896916	21.131078257717174
28	17.585964555223065	28.013966755813918	33.43180871395817	20.968259975004848
29	20.466766944072557	27.177615030162723	31.40230426030573	20.953313765458994
30	18.140564330711918	34.04364929196307	29.88637700700564	17.92940937031937
31	23.226409634263074	25.466433039391212	26.581992679537368	24.72516464680835
32	22.92812145226276	29.605897074676985	28.704036430590758	18.761945042469495
33	21.385163819996755	28.013012742438647	25.752637051971465	24.849186385593125
34	20.53322987588286	27.229449756885593	27.12673431681512	25.11058605041643
35	19.905171070498408	28.332607223153268	25.962201990071897	25.800019716276424
36	24.665061804166495	30.15922483233215	24.900703107857574	20.27501025564378
37	18.807419680023916	27.828570156553596	32.814562060160085	20.549448103262407
38	22.350307351309066	28.00697065772862	25.772035323935246	23.870686667027073
39	21.680907966329688	25.6400634736899	29.888603038214594	22.790425521765815
40	18.884694763420583	27.688012185930848	29.07959969598774	24.347693354660834
41	21.574376472758146	26.41599435224082	25.26927027516926	26.740358899831772
42	18.847170237326726	24.184557067490086	32.396068192876065	24.57220450230712
43	24.814841904083494	25.878884821965205	26.41408632549028	22.892186948461017
44	21.517771679158944	25.127758291171244	29.734052871421255	23.620417158248557
45	19.927431382587983	23.362515542467904	28.408292284257826	28.301760790686288
46	24.69845227230086	28.028912965359776	26.828128130356387	20.444506631982982
47	17.72747653922108	26.2108814765583	35.47371534148909	20.58792664273153
48	21.567698379131276	26.148552602707493	27.516607782841117	24.76714123532012
49	19.657763601845698	22.891868944002596	34.03124711808459	23.419120336067113
50	21.442404622512807	23.449012755158826	29.783979571393594	25.324603050934773
51	19.80881571959639	23.400676077478604	27.631407392331642	29.159100810593365
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	127.0
1	286.5
2	446.0
3	2620.5
4	4795.0
5	3233.0
6	1671.0
7	1570.0
8	1469.0
9	1455.0
10	1441.0
11	1356.0
12	1271.0
13	1237.0
14	1203.0
15	1177.5
16	1152.0
17	1078.0
18	1004.0
19	1095.0
20	1186.0
21	1208.5
22	1231.0
23	1245.5
24	1260.0
25	1746.0
26	2584.0
27	2936.0
28	3680.0
29	4424.0
30	4938.5
31	5453.0
32	6363.0
33	7273.0
34	8049.5
35	8826.0
36	9370.5
37	9915.0
38	10992.5
39	12070.0
40	13194.0
41	14318.0
42	14986.0
43	15654.0
44	16867.5
45	18081.0
46	36183.0
47	54285.0
48	41517.5
49	28750.0
50	28491.0
51	28232.0
52	24040.5
53	19849.0
54	17767.0
55	15685.0
56	13794.0
57	11903.0
58	11228.5
59	10554.0
60	9708.0
61	8862.0
62	7553.0
63	6244.0
64	5229.5
65	4215.0
66	3391.0
67	2567.0
68	1986.0
69	1405.0
70	1224.0
71	1043.0
72	834.0
73	625.0
74	499.5
75	283.5
76	193.0
77	148.0
78	103.0
79	97.0
80	91.0
81	62.5
82	34.0
83	20.0
84	6.0
85	4.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	314461.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.57092774166435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.64504043266462	23.986872904642684
2	10.041564592357705	6.541261495018761
3	3.508508810854056	3.4282616087796045
4	1.8427637586162777	2.4008210090739435
5	1.2750881866021861	2.076540259503481
6	0.9733004070148886	1.9020778336688666
7	0.8553849001171608	1.9502475841118774
8	0.7414667986652851	1.9320209217736146
9	0.6715170992473738	1.968474142519043
>10	6.338441904328403	37.334611755385325
>50	0.06495329083724388	1.387175395798897
>100	0.03197700471987391	2.3056664720411515
>500	0.0	0.0
>1k	0.006994969782472419	4.4040052277228705
>5k	0.002997844192488179	8.381963389959877
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	9196	2.924368999653375	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC	8437	2.6830036157106925	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	8120	2.582196202390757	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2971	0.9447912459732686	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	2509	0.7978731861820703	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTACCGT	2191	0.696747768403713	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCT	1663	0.5288414143566292	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	1596	0.5075351156423213	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTA	1353	0.43026003224565207	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTC	1248	0.3968695641112888	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAGTACCG	495	0.157412206919141	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATG	476	0.1513701222091134	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	469	0.14914409100015583	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGT	409	0.13006382349480541	No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	405	0.12879180566111537	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC	356	0.11320958719841251	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT	343	0.10907552923891993	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	328	0.10430546236258231	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13419788145429798	0.0
2	0.0	0.0	0.0	1.2259071872187648	0.0
3	0.0	0.0	0.0	1.4660005533277576	0.0
4	0.0	0.0	0.0	2.2982182210194586	0.0
5	0.0	0.0	0.0	5.727260296189352	0.0
6	0.0	0.0	0.0	6.289492178680345	0.0
7	0.0	0.0	0.0	7.250819656491584	0.0
8	0.0	0.0	0.0	8.207377067426485	0.0
9	3.180044584225071E-4	0.0	0.0	8.457010567288153	0.0
10	3.180044584225071E-4	0.0	0.0	12.089893500306875	0.0
11	3.180044584225071E-4	0.0	0.0	13.101783687007291	0.0
12	3.180044584225071E-4	0.0	0.0	16.62368306403656	0.0
13	3.180044584225071E-4	0.0	0.0	17.108639863130882	3.180044584225071E-4
14	3.180044584225071E-4	0.0	0.0	17.344917175738804	3.180044584225071E-4
15	3.180044584225071E-4	0.0	0.0	18.063289247315247	3.180044584225071E-4
16	3.180044584225071E-4	0.0	0.0	18.684351954614403	3.180044584225071E-4
17	3.180044584225071E-4	0.0	0.0	19.35470535296905	3.180044584225071E-4
18	3.180044584225071E-4	0.0	0.0	19.956687792762853	3.180044584225071E-4
19	3.180044584225071E-4	0.0	0.0	21.11581404371289	3.180044584225071E-4
20	3.180044584225071E-4	0.0	0.0	21.714616438922473	3.180044584225071E-4
21	3.180044584225071E-4	0.0	0.0	22.228193639274824	3.180044584225071E-4
22	3.180044584225071E-4	0.0	0.0	22.866428587328794	3.180044584225071E-4
23	3.180044584225071E-4	0.0	0.0	23.43502055898824	3.180044584225071E-4
24	3.180044584225071E-4	0.0	0.0	23.88340684536397	3.180044584225071E-4
25	3.180044584225071E-4	0.0	0.0	24.28027640947526	3.180044584225071E-4
26	3.180044584225071E-4	0.0	0.0	24.659019719456467	3.180044584225071E-4
27	3.180044584225071E-4	0.0	0.0	25.114402103917495	3.180044584225071E-4
28	3.180044584225071E-4	0.0	0.0	25.526853886491487	3.180044584225071E-4
29	3.180044584225071E-4	0.0	0.0	25.95584190090345	3.180044584225071E-4
30	3.180044584225071E-4	0.0	0.0	26.42521648153507	3.180044584225071E-4
31	3.180044584225071E-4	0.0	0.0	26.828128130356387	3.180044584225071E-4
32	3.180044584225071E-4	0.0	0.0	27.264430247312067	3.180044584225071E-4
33	3.180044584225071E-4	0.0	0.0	27.677518038802905	3.180044584225071E-4
34	3.180044584225071E-4	0.0	0.0	28.110004102257513	3.180044584225071E-4
35	3.180044584225071E-4	0.0	0.0	28.556482361882715	3.180044584225071E-4
36	3.180044584225071E-4	0.0	0.0	29.01536279538639	3.180044584225071E-4
37	3.180044584225071E-4	0.0	0.0	29.471381188764266	3.180044584225071E-4
38	3.180044584225071E-4	0.0	0.0	29.920085479598423	3.180044584225071E-4
39	3.180044584225071E-4	0.0	0.0	30.405042278692747	3.180044584225071E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	30	2.1604537E-6	45.000004	34
CTACGAA	65	0.0	45.000004	11
TACGAAT	65	0.0	45.000004	12
TGCTATA	30	2.1604537E-6	45.000004	32
AGCGTTC	20	7.025463E-4	45.0	7
ATTAGTC	25	3.8843227E-5	45.0	31
GCGACAC	20	7.025463E-4	45.0	33
CTCAACG	20	7.025463E-4	45.0	1
GTCGAAT	95	0.0	45.0	43
GTACACG	20	7.025463E-4	45.0	1
CGGCCTT	25	3.8843227E-5	45.0	44
GCGAACC	35	1.2081728E-7	45.0	33
TAGCGCG	25	3.8843227E-5	45.0	1
ACTCTCG	20	7.025463E-4	45.0	33
TTGTCCG	20	7.025463E-4	45.0	25
TTCGTAC	20	7.025463E-4	45.0	33
CGATTGG	25	3.8843227E-5	45.0	2
ACGGGCC	40	6.7884685E-9	45.0	5
CCGTCGA	95	0.0	45.0	41
CCCACGC	20	7.025463E-4	45.0	39
>>END_MODULE