SRR3551872_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551872_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	459915
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	15985	3.4756422382396748	Illumina PCR Primer Index 5 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	15339	3.3351815009295196	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	14803	3.218638226628834	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	4653	1.0117086853005446	RNA PCR Primer, Index 33 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCT	4516	0.9819205722796603	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3621	0.7873193959753434	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	3500	0.7610101866649273	RNA PCR Primer, Index 33 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	2881	0.6264200993661873	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTA	2372	0.5157474750769164	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTC	1900	0.41311981561810335	RNA PCR Primer, Index 33 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1269	0.2759205505365122	Illumina PCR Primer Index 5 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	962	0.20916908559190286	Illumina PCR Primer Index 5 (96% over 26bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGGTGATC	879	0.19112227259384884	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATG	811	0.17633693182435883	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	794	0.17264059663198633	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGT	763	0.16590022069295413	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	608	0.13219834099779307	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTAT	565	0.12284878727590968	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	534	0.11610841133687747	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	468	0.10175793353119598	Illumina PCR Primer Index 5 (95% over 24bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	35	1.2098099E-7	45.000004	4
CGAAAGG	45	3.8380676E-10	45.0	2
GCACCGC	45	3.8380676E-10	45.0	37
GTCGTAG	20	7.0288696E-4	45.0	1
TTGCGCG	25	3.8871432E-5	45.0	1
AGTTCGG	25	3.8871432E-5	45.0	2
TTACGCG	20	7.0288696E-4	45.0	1
GCTACGA	50	2.1827873E-11	45.0	10
TGTAACG	40	6.7993824E-9	45.0	1
AACACCG	25	3.8871432E-5	45.0	36
ACGGCTA	30	2.162651E-6	44.999996	30
CCGCGCA	30	2.162651E-6	44.999996	40
GACGAAT	30	2.162651E-6	44.999996	20
GCTCGAA	60	0.0	44.999996	42
CGTTTTT	1990	0.0	43.643215	1
CGAATGC	150	0.0	43.5	45
TACGGCT	1635	0.0	43.348625	7
ACGGCTG	1635	0.0	43.348625	8
TGATACC	1605	0.0	42.61682	4
GATGAAT	940	0.0	42.12766	20

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content