##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551872_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	459915
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.783446941282627	30.0	25.0	31.0	16.0	33.0
2	27.82699846710805	30.0	25.0	33.0	16.0	33.0
3	27.673761455921202	30.0	25.0	33.0	16.0	34.0
4	31.94340258526032	35.0	30.0	35.0	19.0	37.0
5	33.39143537392779	35.0	32.0	35.0	28.0	37.0
6	33.29300631638455	35.0	33.0	35.0	28.0	37.0
7	33.961236315406104	35.0	33.0	36.0	31.0	37.0
8	33.665059848015396	35.0	33.0	37.0	28.0	37.0
9	35.835145624735006	38.0	35.0	39.0	30.0	39.0
10	35.282439146364005	37.0	34.0	39.0	31.0	39.0
11	36.004737831990695	37.0	35.0	39.0	32.0	39.0
12	36.08463737864606	37.0	35.0	39.0	32.0	39.0
13	36.085250535425025	37.0	35.0	39.0	32.0	39.0
14	37.151106182664186	39.0	36.0	40.0	32.0	41.0
15	37.13365730624137	39.0	36.0	40.0	32.0	41.0
16	36.793742321950795	38.0	35.0	40.0	32.0	41.0
17	36.695132796277576	38.0	35.0	40.0	31.0	41.0
18	36.710092082232585	38.0	35.0	40.0	32.0	41.0
19	36.37181435700075	38.0	34.0	40.0	31.0	41.0
20	36.23252557537806	38.0	35.0	40.0	30.0	41.0
21	36.79952599936945	38.0	35.0	40.0	32.0	41.0
22	37.11897198395356	39.0	35.0	40.0	32.0	41.0
23	37.40018916538926	39.0	35.0	40.0	32.0	41.0
24	37.40433340943435	39.0	35.0	41.0	32.0	41.0
25	36.71244686518161	38.0	35.0	40.0	31.0	41.0
26	36.824032701694875	39.0	35.0	40.0	31.0	41.0
27	37.05910439972604	39.0	35.0	40.0	32.0	41.0
28	37.05273800593588	39.0	35.0	40.0	31.0	41.0
29	37.010891142928585	39.0	35.0	40.0	31.0	41.0
30	36.71162932280965	39.0	35.0	40.0	31.0	41.0
31	36.784360153506626	39.0	35.0	40.0	31.0	41.0
32	36.4922126914756	39.0	35.0	40.0	30.0	41.0
33	36.57547155452638	39.0	35.0	40.0	30.0	41.0
34	36.251450811563004	39.0	35.0	41.0	29.0	41.0
35	36.42018633877999	39.0	35.0	41.0	30.0	41.0
36	36.39535783786135	39.0	35.0	41.0	30.0	41.0
37	36.220977789374125	39.0	35.0	41.0	30.0	41.0
38	36.18282508724438	39.0	35.0	40.0	30.0	41.0
39	35.86842133872563	39.0	35.0	40.0	28.0	41.0
40	35.85741278279682	39.0	35.0	40.0	28.0	41.0
41	35.667208071056606	39.0	35.0	40.0	26.0	41.0
42	36.03427807312221	39.0	35.0	40.0	29.0	41.0
43	36.151634541165215	39.0	35.0	40.0	30.0	41.0
44	36.36118630616527	39.0	35.0	40.0	30.0	41.0
45	36.4276551101834	39.0	35.0	40.0	31.0	41.0
46	36.1857386691019	39.0	35.0	40.0	30.0	41.0
47	35.94139569268234	39.0	35.0	40.0	29.0	41.0
48	36.111938075513955	39.0	35.0	40.0	30.0	41.0
49	36.17779589706794	39.0	35.0	40.0	30.0	41.0
50	36.0180750790907	39.0	35.0	40.0	29.0	41.0
51	35.10646532511442	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	10.0
10	10.0
11	16.0
12	9.0
13	19.0
14	15.0
15	26.0
16	36.0
17	96.0
18	228.0
19	520.0
20	1106.0
21	1679.0
22	2246.0
23	2753.0
24	3754.0
25	4916.0
26	5695.0
27	5906.0
28	6373.0
29	7129.0
30	9178.0
31	12072.0
32	16877.0
33	24656.0
34	32641.0
35	39294.0
36	58289.0
37	91323.0
38	103000.0
39	30041.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.29864214039551	16.396290618918712	17.8893926051553	15.415674635530479
2	29.12603415848581	35.6611547786004	20.153724057706317	15.059087005207484
3	26.99911940249829	33.90843960297011	25.40643379754955	13.68600719698205
4	24.9554809040801	20.195036039268125	38.56669167128708	16.282791385364686
5	33.11111835882718	23.50956154941674	23.8948501353511	19.484469956404986
6	21.499190067729906	39.289868780100676	25.634301990585218	13.576639161584206
7	64.76218431666722	5.574073470097735	24.558016155159105	5.105726058075949
8	61.98667145015927	16.709826815824663	14.448321972538402	6.855179761477665
9	56.96617853298979	7.2959133752976095	14.530293641216312	21.207614450496287
10	37.658697802854874	22.966417707619886	23.510431275344356	15.864453214180882
11	30.322342171923072	21.581814030853526	28.357196438472325	19.738647358751074
12	23.753519672113324	18.657360599241162	32.10397573464662	25.485143993998893
13	24.22077992672559	20.4944391898503	38.05159649065588	17.233184392768226
14	19.953687094354393	29.501538327734472	28.213691660415513	22.331082917495625
15	16.37563462813781	23.727427894284816	39.378580824717616	20.51835665285977
16	17.980496396073185	25.70844612591457	27.69403041866432	28.617027059347922
17	18.064424948088234	27.14588565278367	33.26940847765348	21.520280921474622
18	17.610428013872127	25.858038985464702	31.899807573138517	24.63172542752465
19	18.47667503777872	26.487720557059458	29.722666144831113	25.312938260330714
20	20.54944935477208	27.04804148592675	36.187556396290624	16.214952763010558
21	20.517704358414054	31.741517454312213	30.440624898078994	17.300153289194743
22	17.668047356576757	22.278029635910983	34.245675831403624	25.80824717610863
23	20.075883587184588	28.26152658643445	32.24813280714915	19.414457019231815
24	21.61247186980203	24.733918224019654	28.39763869410652	25.2559712120718
25	17.346466194840353	35.19454681843384	26.576432601676398	20.882554385049414
26	17.64673907135014	25.852385766935193	31.819792787797745	24.68108237391692
27	24.210125784112282	27.141537023145577	27.00846895622017	21.639868236521966
28	17.16599806485981	26.810823739169194	33.65339247469641	22.36978572127458
29	22.013415522433494	26.075905330332777	29.86899753215268	22.041681615081046
30	23.875933596425426	28.66529684833067	29.373036321929053	18.08573323331485
31	26.037854820999534	24.875900981703143	25.351423632627768	23.73482056466956
32	24.35189111031386	27.844275572660166	29.865736059924114	17.938097257101855
33	25.172042660056746	23.040561842949238	24.61541806638183	27.171977430612177
34	21.86947588141287	30.556515877933965	27.142841612037007	20.431166628616158
35	23.564136851374712	23.24081623778307	29.848124109889874	23.34692280095235
36	23.814183055564612	28.83641542458933	24.334931454725332	23.01447006512073
37	22.688540273746234	23.77200134807519	34.17609775719426	19.363360620984313
38	26.885837600426164	23.36431731950469	25.429046671667592	24.320798408401554
39	22.96815715947512	26.986508376547842	30.160790580868202	19.884543883108837
40	23.65741495711164	22.9618516464999	32.69495450246241	20.685778893926052
41	19.464031397105988	29.59003294086951	25.670613048063228	25.275322613961276
42	19.470119478599308	22.385223356489785	36.548275224769796	21.596381940141114
43	23.67676635900112	23.047954513333984	26.131785221182174	27.143493906482718
44	23.48042573084157	22.59591446245502	29.680049574377875	24.243610232325537
45	21.736842677451268	21.333072415555048	28.356544144026614	28.57354076296707
46	26.6631877629562	25.911961992976963	27.415935553308763	20.008914690758075
47	19.385973495102355	22.811389061022144	37.55324353413131	20.249393909744192
48	21.37003576747877	26.355087353097854	27.35048867725558	24.92438820216779
49	20.093930400182643	20.289836165378386	37.271452333583376	22.34478110085559
50	22.435667460291576	20.44964830457802	30.950719154626398	26.163965080504003
51	20.917560853635997	21.152821717056412	27.829272800408773	30.100344628898817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118.0
1	314.0
2	510.0
3	3351.0
4	6192.0
5	4258.0
6	2324.0
7	2161.0
8	1998.0
9	1997.0
10	1996.0
11	1882.5
12	1769.0
13	1762.5
14	1756.0
15	1666.5
16	1577.0
17	1507.5
18	1438.0
19	1407.0
20	1376.0
21	1495.5
22	1615.0
23	1679.5
24	1744.0
25	2224.5
26	3098.0
27	3491.0
28	4210.0
29	4929.0
30	5767.0
31	6605.0
32	7536.5
33	8468.0
34	9203.5
35	9939.0
36	11007.5
37	12076.0
38	13450.5
39	14825.0
40	16790.5
41	18756.0
42	20231.5
43	21707.0
44	23756.0
45	25805.0
46	58234.5
47	90664.0
48	69123.0
49	47582.0
50	46227.5
51	44873.0
52	37249.0
53	29625.0
54	26332.0
55	23039.0
56	20563.0
57	18087.0
58	16152.5
59	14218.0
60	13174.5
61	12131.0
62	10857.0
63	9583.0
64	7897.5
65	6212.0
66	5026.0
67	3840.0
68	3122.5
69	2405.0
70	1996.0
71	1587.0
72	1313.0
73	1039.0
74	815.5
75	447.0
76	302.0
77	250.0
78	198.0
79	153.0
80	108.0
81	88.0
82	68.0
83	47.5
84	27.0
85	18.5
86	10.0
87	6.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	459915.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.28014942289658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.13587223449103	27.25940671687001
2	9.550468018754373	6.929848135580073
3	3.4950955911982504	3.804077708879387
4	1.971924960754605	2.8616692890766617
5	1.3695813784981898	2.48443085293655
6	1.17048734473879	2.5479273458839655
7	0.9317194730937718	2.3662045192845156
8	0.8251473101210804	2.3949174165675187
9	0.7224169854779408	2.3588456560900384
>10	4.734576045647368	27.10378806066846
>50	0.05418155292344382	1.3805870531697149
>100	0.02709077646172191	1.7440258850751738
>500	0.004816138037639452	1.2921298855484942
>1k	0.004816138037639452	5.3974161142113575
>5k	0.0	0.0
>10k+	0.0018060517641147944	10.074725360158112
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	15985	3.4756422382396748	Illumina PCR Primer Index 5 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	15339	3.3351815009295196	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	14803	3.218638226628834	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	4653	1.0117086853005446	RNA PCR Primer, Index 33 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCT	4516	0.9819205722796603	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3621	0.7873193959753434	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	3500	0.7610101866649273	RNA PCR Primer, Index 33 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	2881	0.6264200993661873	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTA	2372	0.5157474750769164	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTC	1900	0.41311981561810335	RNA PCR Primer, Index 33 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1269	0.2759205505365122	Illumina PCR Primer Index 5 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	962	0.20916908559190286	Illumina PCR Primer Index 5 (96% over 26bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGGTGATC	879	0.19112227259384884	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATG	811	0.17633693182435883	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	794	0.17264059663198633	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGT	763	0.16590022069295413	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	608	0.13219834099779307	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTAT	565	0.12284878727590968	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	534	0.11610841133687747	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	468	0.10175793353119598	Illumina PCR Primer Index 5 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21656175597664787	0.0
2	0.0	0.0	0.0	1.6842242588304361	0.0
3	0.0	0.0	0.0	2.022982507637281	0.0
4	0.0	0.0	0.0	3.1518867616842243	0.0
5	0.0	0.0	0.0	7.7170781557461705	0.0
6	0.0	0.0	0.0	8.537012274007154	0.0
7	0.0	0.0	0.0	9.73179826707109	0.0
8	0.0	0.0	0.0	10.818085950664797	0.0
9	0.0	0.0	0.0	11.159018514290684	0.0
10	0.0	0.0	0.0	15.2639074611613	0.0
11	0.0	0.0	0.0	16.584151419283998	0.0
12	0.0	0.0	0.0	21.11759781698792	0.0
13	0.0	0.0	0.0	21.750105997847427	0.0
14	0.0	0.0	0.0	22.082993596642858	0.0
15	0.0	0.0	0.0	22.986638835436985	0.0
16	0.0	0.0	0.0	23.793961927747517	0.0
17	0.0	0.0	0.0	24.632595153452268	0.0
18	0.0	0.0	0.0	25.38099431416675	0.0
19	0.0	0.0	0.0	26.945631257949838	0.0
20	0.0	0.0	0.0	27.63032299446637	0.0
21	0.0	0.0	0.0	28.248480697520193	0.0
22	0.0	0.0	0.0	29.017318417533676	0.0
23	0.0	0.0	0.0	29.68439820401596	0.0
24	0.0	0.0	0.0	30.221453964319494	0.0
25	0.0	0.0	0.0	30.712414250459325	0.0
26	0.0	0.0	0.0	31.174891012469697	0.0
27	0.0	0.0	0.0	31.705641259798007	0.0
28	0.0	0.0	0.0	32.194644662600695	0.0
29	0.0	0.0	0.0	32.66429666351391	0.0
30	0.0	0.0	0.0	33.172216605242276	0.0
31	0.0	0.0	0.0	33.6529576117326	0.0
32	0.0	0.0	0.0	34.1067371144668	0.0
33	0.0	0.0	0.0	34.567039561658135	0.0
34	0.0	0.0	0.0	35.038865877390386	0.0
35	2.1743148190426492E-4	0.0	0.0	35.52678212278356	0.0
36	2.1743148190426492E-4	0.0	0.0	35.99034604220345	0.0
37	2.1743148190426492E-4	0.0	0.0	36.46217235793571	0.0
38	2.1743148190426492E-4	0.0	0.0	36.94878401443745	0.0
39	2.1743148190426492E-4	0.0	0.0	37.43843971168585	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	35	1.2098099E-7	45.000004	4
CGAAAGG	45	3.8380676E-10	45.0	2
GCACCGC	45	3.8380676E-10	45.0	37
GTCGTAG	20	7.0288696E-4	45.0	1
TTGCGCG	25	3.8871432E-5	45.0	1
AGTTCGG	25	3.8871432E-5	45.0	2
TTACGCG	20	7.0288696E-4	45.0	1
GCTACGA	50	2.1827873E-11	45.0	10
TGTAACG	40	6.7993824E-9	45.0	1
AACACCG	25	3.8871432E-5	45.0	36
ACGGCTA	30	2.162651E-6	44.999996	30
CCGCGCA	30	2.162651E-6	44.999996	40
GACGAAT	30	2.162651E-6	44.999996	20
GCTCGAA	60	0.0	44.999996	42
CGTTTTT	1990	0.0	43.643215	1
CGAATGC	150	0.0	43.5	45
TACGGCT	1635	0.0	43.348625	7
ACGGCTG	1635	0.0	43.348625	8
TGATACC	1605	0.0	42.61682	4
GATGAAT	940	0.0	42.12766	20
>>END_MODULE