##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551871_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	305927
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.69548944682882	30.0	25.0	31.0	16.0	33.0
2	27.794460770052986	30.0	25.0	33.0	16.0	33.0
3	27.68967433407316	30.0	25.0	33.0	16.0	34.0
4	31.579543485864274	35.0	28.0	35.0	19.0	37.0
5	33.315379159080436	35.0	32.0	35.0	28.0	37.0
6	33.30667446809206	35.0	33.0	35.0	28.0	37.0
7	33.80577719521324	35.0	33.0	36.0	30.0	37.0
8	33.741810954901005	35.0	33.0	37.0	29.0	37.0
9	35.994181618490686	39.0	35.0	39.0	31.0	39.0
10	35.42578131384285	37.0	34.0	39.0	31.0	39.0
11	36.11360880209985	37.0	35.0	39.0	32.0	39.0
12	36.165147241008476	37.0	35.0	39.0	32.0	39.0
13	36.24680397611195	37.0	35.0	39.0	32.0	39.0
14	37.33329846662766	39.0	36.0	40.0	32.0	41.0
15	37.35119162414563	39.0	36.0	40.0	32.0	41.0
16	37.0092113478052	38.0	35.0	40.0	32.0	41.0
17	36.954930424578414	38.0	36.0	40.0	32.0	41.0
18	36.916133587424454	38.0	36.0	40.0	32.0	41.0
19	36.508134293475244	38.0	34.0	40.0	31.0	41.0
20	36.42902391747051	38.0	35.0	40.0	31.0	41.0
21	36.92557047923197	39.0	35.0	40.0	32.0	41.0
22	37.23924988641081	39.0	35.0	40.0	32.0	41.0
23	37.49835418253374	39.0	35.0	40.0	33.0	41.0
24	37.5338430409869	39.0	35.0	41.0	33.0	41.0
25	36.7836248516803	38.0	35.0	40.0	31.0	41.0
26	36.91220781428249	39.0	35.0	40.0	31.0	41.0
27	37.15519715487682	39.0	35.0	40.0	32.0	41.0
28	37.18529256979606	39.0	35.0	40.0	32.0	41.0
29	37.20791234510194	39.0	35.0	41.0	32.0	41.0
30	36.94911531182275	39.0	35.0	40.0	31.0	41.0
31	36.94163313470207	39.0	35.0	40.0	31.0	41.0
32	36.80614002686916	39.0	35.0	40.0	31.0	41.0
33	36.715200031380036	39.0	36.0	41.0	30.0	41.0
34	36.5153909265936	40.0	35.0	41.0	30.0	41.0
35	36.46496713268198	40.0	35.0	41.0	30.0	41.0
36	36.311133048080094	40.0	35.0	41.0	29.0	41.0
37	36.21838870057236	39.0	35.0	41.0	30.0	41.0
38	36.177705792558356	39.0	35.0	41.0	30.0	41.0
39	36.00490966799269	39.0	35.0	41.0	28.0	41.0
40	35.86676233219036	39.0	35.0	40.0	27.0	41.0
41	35.77341653400975	39.0	35.0	40.0	26.0	41.0
42	35.9464414713314	39.0	35.0	41.0	28.0	41.0
43	35.96231453908939	39.0	35.0	41.0	28.0	41.0
44	36.07766885564203	39.0	35.0	41.0	29.0	41.0
45	36.116691236798324	39.0	35.0	40.0	29.0	41.0
46	35.967302657169846	39.0	35.0	40.0	28.0	41.0
47	35.7229796650835	39.0	35.0	40.0	26.0	41.0
48	35.90785056565782	39.0	35.0	40.0	28.0	41.0
49	35.91729726372631	39.0	35.0	40.0	28.0	41.0
50	35.757903029154015	39.0	35.0	40.0	27.0	41.0
51	34.87259705746796	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	15.0
11	11.0
12	12.0
13	17.0
14	13.0
15	15.0
16	44.0
17	63.0
18	149.0
19	355.0
20	671.0
21	1013.0
22	1411.0
23	1875.0
24	2788.0
25	3888.0
26	4381.0
27	4477.0
28	4594.0
29	4737.0
30	5802.0
31	7564.0
32	10064.0
33	14617.0
34	20125.0
35	25245.0
36	37411.0
37	60198.0
38	72611.0
39	21758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.46099559698882	16.970715235987672	17.821571812883466	17.74671735414004
2	31.06427350315598	33.50799373706799	18.81298479702674	16.61474796274928
3	24.918362877418467	31.46208082320292	28.07499828390433	15.54455801547428
4	24.103789466114463	21.032141654708475	38.875287241727605	15.988781637449456
5	30.724976873567876	23.59288326953816	26.512860911263143	19.169278945630822
6	21.30116008067284	36.90063315758334	28.262624743811433	13.535582017932382
7	64.81742376449283	5.649386945251645	24.53297682126782	5.0002124689877
8	62.429926093479814	14.235095300512867	16.469288425016426	6.86569018099089
9	57.46861179300944	8.121872211344536	16.56669728399259	17.842818711653432
10	34.33368091080552	24.54212933150719	25.945732151787844	15.178457605899446
11	27.367313117181553	22.331471233333442	30.57396045461826	19.72725519486675
12	23.078708319304933	18.228858518535468	34.837722724702296	23.854710437457303
13	22.87669934330739	20.66015748855119	39.31853023760571	17.144612930535715
14	18.98753624230617	28.34172858230885	31.025048459272963	21.645686716112014
15	16.361746429703818	24.27932153749097	40.04027104505323	19.31866098775198
16	17.378328817005364	25.964364047632277	30.933196481513566	25.724110653848793
17	18.152696558329275	26.543587195638175	34.43599290026706	20.867723345765494
18	16.771975013647047	26.38014951279227	34.36996407639731	22.477911397163375
19	17.499599577677028	27.404250033504724	31.416318272006066	23.67983211681218
20	19.497788688151093	26.854772543776782	37.621066463568106	16.026372304504015
21	20.28228956581145	29.720161999431237	32.72578098696748	17.27176744778983
22	17.24986679828848	22.92311564523563	35.305154497641595	24.5218630588343
23	18.427925616241783	27.681767218977075	34.38500034321913	19.50530682156201
24	20.648063100020593	24.927188512292147	30.589977347537157	23.834771040150102
25	16.36926456311473	33.59265445678217	29.26351711355977	20.774563866543325
26	16.593174188613624	26.794954351855182	33.63678263115057	22.975088828380628
27	21.332213240413562	28.176329647268794	29.784556446472525	20.706900665845122
28	15.23108453977583	28.609112631444756	34.588316820679445	21.57148600809997
29	19.24347965364286	27.38627188839168	32.52344513560425	20.846803322361218
30	18.552465130570365	33.040235088763005	30.66842743530319	17.738872345363436
31	19.303624720930156	29.124921958506437	27.622275902421165	23.949177418142238
32	22.374618781604763	29.730295135767683	30.045402988294594	17.84968309433296
33	23.06530642931157	25.214838834100945	26.5903303729321	25.12952436365538
34	20.317265229940475	27.643522801191132	28.188424035799393	23.850787933069
35	23.239204123859615	27.57782085268708	26.934857008371278	22.24811801508203
36	22.51321393665809	32.335491800331454	25.47699287738578	19.674301385624677
37	18.287369208994303	28.467575598100197	33.98882740000065	19.25622779290484
38	23.266661654577725	28.59832574437693	25.907487734001904	22.227524867043442
39	22.769157347994785	24.927515387657838	30.515449764159424	21.787877500187953
40	25.151751888522426	23.949177418142238	29.57339496023561	21.32567573309973
41	22.537075838353594	27.2895167801469	25.650563696568135	24.522843684931374
42	23.435329343274702	24.5695868622253	31.764440536467852	20.230643258032146
43	25.879376452552407	24.71079702020417	27.484661373464913	21.92516515377852
44	21.025931022760332	25.171691285829628	29.85025839497658	23.952119296433462
45	20.480049162055	23.491551906173694	28.502878137594916	27.52552079417639
46	25.310286440882955	26.793319975026726	27.984126932242003	19.912266651848316
47	19.077426967871418	24.518267429811686	36.37599819564798	20.02830740666891
48	21.18250432292671	26.120283597067274	27.628813409735002	25.068398670271012
49	20.64152559270676	22.004595867641626	35.401909605886374	21.951968933765244
50	22.539363965913438	21.85292569796062	31.39932075299009	24.208389583135844
51	21.20538559852514	21.458714006936294	28.425081800560264	28.910818593978306
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118.0
1	284.0
2	450.0
3	3119.5
4	5789.0
5	3969.5
6	2150.0
7	2050.5
8	1951.0
9	1883.0
10	1815.0
11	1772.5
12	1730.0
13	1704.5
14	1679.0
15	1593.5
16	1508.0
17	1412.5
18	1317.0
19	1382.5
20	1448.0
21	1396.0
22	1344.0
23	1469.0
24	1594.0
25	2040.0
26	2670.0
27	2854.0
28	3453.0
29	4052.0
30	4601.5
31	5151.0
32	5916.5
33	6682.0
34	7494.0
35	8306.0
36	8867.5
37	9429.0
38	10471.0
39	11513.0
40	12646.5
41	13780.0
42	14561.5
43	15343.0
44	16947.5
45	18552.0
46	36797.0
47	55042.0
48	42698.5
49	30355.0
50	29398.5
51	28442.0
52	23771.0
53	19100.0
54	16402.5
55	13705.0
56	11804.5
57	9904.0
58	8899.0
59	7894.0
60	7288.5
61	6683.0
62	5769.5
63	4856.0
64	4156.5
65	3457.0
66	2678.0
67	1899.0
68	1587.0
69	1275.0
70	1059.0
71	843.0
72	675.0
73	507.0
74	452.5
75	314.5
76	231.0
77	178.5
78	126.0
79	108.0
80	90.0
81	68.5
82	47.0
83	35.5
84	24.0
85	14.0
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	305927.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.30678397765067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8175564402864	30.962686531296114
2	9.316975910092374	7.510746706661371
3	3.448704832312568	4.170186020361079
4	1.8834610580615874	3.0366503199042305
5	1.3551420248230062	2.731070842678852
6	0.9779831099607323	2.3651612368186927
7	0.8778733096163202	2.4768974895315177
8	0.677079208035539	2.1832708299237815
9	0.6396847435131177	2.3205271293523895
>10	3.921006884271152	25.076248875346234
>50	0.045517488706961845	1.2737807172501414
>100	0.02844843044185115	2.185215376558242
>500	0.002438436895015813	0.6758769597960499
>1k	0.005689686088370231	4.881843227300504
>5k	0.002438436895015813	8.149837737220812
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTC	8743	2.8578713222435415	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCG	8294	2.711104283047917	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGC	7839	2.562375991658141	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3404	1.1126837448149396	No Hit
GCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	2678	0.8753722293226817	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAATGCGGT	2300	0.7518133410911754	No Hit
GAACTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCT	1925	0.6292350789567446	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCT	1800	0.5883756582452677	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAATGCGGTCGTA	1412	0.4615480163568433	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTC	1382	0.45174175538608885	No Hit
CCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	787	0.25725091279945866	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCC	748	0.2445027735374779	No Hit
CTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCT	528	0.1725901930852785	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAATGCGG	456	0.14905516675546782	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATG	441	0.14415203627009057	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAATGCGGTCGT	395	0.12911576944826705	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTT	369	0.12061700994027987	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTAT	306	0.1000238619016955	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1892608367355611	0.0
2	0.0	0.0	0.0	1.573904885806091	0.0
3	0.0	0.0	0.0	1.9403321707466161	0.0
4	0.0	0.0	0.0	3.0425559038594177	0.0
5	0.0	0.0	0.0	7.07456353966796	0.0
6	0.0	0.0	0.0	7.847950654894795	0.0
7	0.0	0.0	0.0	9.043660742595456	0.0
8	0.0	0.0	0.0	10.300496523680486	0.0
9	0.0	0.0	0.0	10.661693802769943	0.0
10	0.0	0.0	0.0	14.265167834156514	0.0
11	0.0	0.0	0.0	15.771736394630091	0.0
12	0.0	0.0	0.0	19.499749940345247	0.0
13	0.0	0.0	0.0	20.171151941476236	0.0
14	3.268753656918154E-4	0.0	0.0	20.500642310093586	0.0
15	3.268753656918154E-4	0.0	0.0	21.321426353345732	0.0
16	3.268753656918154E-4	0.0	0.0	22.12227099929068	0.0
17	3.268753656918154E-4	0.0	0.0	22.99502822568783	0.0
18	3.268753656918154E-4	0.0	0.0	23.83640541697856	0.0
19	3.268753656918154E-4	0.0	0.0	25.250795124327045	0.0
20	3.268753656918154E-4	0.0	0.0	26.025162865650955	0.0
21	3.268753656918154E-4	0.0	0.0	26.712581759700843	0.0
22	3.268753656918154E-4	0.0	0.0	27.518002660765475	0.0
23	3.268753656918154E-4	0.0	0.0	28.272431004782188	0.0
24	3.268753656918154E-4	0.0	0.0	28.86865167180406	0.0
25	3.268753656918154E-4	0.0	0.0	29.417802286166307	0.0
26	3.268753656918154E-4	0.0	0.0	29.890464064956674	0.0
27	3.268753656918154E-4	0.0	0.0	30.418367780548955	0.0
28	3.268753656918154E-4	0.0	0.0	30.932215855416487	0.0
29	3.268753656918154E-4	0.0	0.0	31.45260143759786	0.0
30	3.268753656918154E-4	0.0	0.0	32.02659457975269	0.0
31	3.268753656918154E-4	0.0	0.0	32.55024891559098	0.0
32	3.268753656918154E-4	0.0	0.0	33.039581338031624	0.0
33	3.268753656918154E-4	0.0	0.0	33.5276062590095	0.0
34	3.268753656918154E-4	0.0	0.0	34.04733809045949	0.0
35	3.268753656918154E-4	0.0	0.0	34.5621667914241	0.0
36	3.268753656918154E-4	0.0	0.0	35.060978599469806	0.0
37	3.268753656918154E-4	0.0	0.0	35.586267312136556	0.0
38	3.268753656918154E-4	0.0	0.0	36.07461910848013	0.0
39	6.537507313836308E-4	0.0	0.0	36.60252282407241	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGG	20	7.025161E-4	45.000004	2
CACGGTT	20	7.025161E-4	45.000004	4
GTCGAAT	30	2.160261E-6	45.000004	43
GCACGGT	20	7.025161E-4	45.000004	3
TACGGTT	20	7.025161E-4	45.000004	33
GTGGCGT	20	7.025161E-4	45.000004	34
AAAACCG	20	7.025161E-4	45.000004	36
TACGCGG	20	7.025161E-4	45.000004	2
GTTCGCG	20	7.025161E-4	45.000004	1
TGCGATA	20	7.025161E-4	45.000004	32
TACGAAA	20	7.025161E-4	45.000004	20
TCGTACG	20	7.025161E-4	45.000004	30
ACATACG	20	7.025161E-4	45.000004	17
ACGGTTA	20	7.025161E-4	45.000004	34
CGAACCC	25	3.884072E-5	45.0	34
CGAACAC	25	3.884072E-5	45.0	34
CGCGGGC	25	3.884072E-5	45.0	4
GCGAACC	25	3.884072E-5	45.0	33
CCAGCGG	25	3.884072E-5	45.0	2
CGTTAGG	25	3.884072E-5	45.0	2
>>END_MODULE