FastQCFastQC Report
Sat 18 Jun 2016
SRR3551870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352899
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG87152.4695451106407216No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC87002.4652946027050233No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC79352.248518697984409No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32190.9121590030008586No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC25440.7208861458944343No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAACTAGT21560.6109396739577045No Hit
GAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT20690.5862867279306544No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT18150.5143114602194963No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC14500.41088243378417055No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTA14140.40068121473849455No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC6420.1819217396478879No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT5540.15698542642512447No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATG5280.14961787933658072No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGAACTAG5160.14621747298802207No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC4680.13261584759378747No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT4300.12184789415668505No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGT4300.12184789415668505No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG253.885293E-545.04
ATTTACG253.885293E-545.035
CGGGTGA253.885293E-545.06
TTTACGC253.885293E-545.036
CGTTGAT207.026635E-445.025
ATAGCGG253.885293E-545.02
TCTAGTA551.8189894E-1245.019
TTCCCGT207.026635E-445.038
TCGTGTA253.885293E-545.017
CGAATAC207.026635E-445.045
TGCTCGG406.7921064E-945.02
CGTAAGG207.026635E-445.02
TTACGCA253.885293E-545.037
TAAGGGC453.8380676E-1045.04
GCTAACG453.8380676E-1045.01
TATCGTG253.885293E-545.01
AGTACGG207.026635E-445.02
AGTCGAA351.2087366E-745.042
GTGCTCG302.1612104E-644.9999961
ATCGCGG302.1612104E-644.9999962
Produced by FastQC (version 0.11.5)