##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551870_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	352899
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.662169629270696	30.0	25.0	31.0	16.0	33.0
2	27.75324101230097	30.0	25.0	33.0	16.0	33.0
3	27.52664926792085	30.0	25.0	33.0	16.0	34.0
4	31.735255696389054	35.0	28.0	35.0	19.0	37.0
5	33.287977579987476	35.0	32.0	35.0	28.0	37.0
6	33.22573030810515	35.0	32.0	35.0	28.0	37.0
7	33.8622552061638	35.0	33.0	36.0	30.0	37.0
8	33.697958906089276	35.0	33.0	37.0	28.0	37.0
9	35.88950379570359	39.0	35.0	39.0	30.0	39.0
10	35.358706032037496	37.0	34.0	39.0	31.0	39.0
11	36.1024854136736	37.0	35.0	39.0	32.0	39.0
12	36.17632523753255	37.0	35.0	39.0	32.0	39.0
13	36.19107733374138	37.0	35.0	39.0	32.0	39.0
14	37.19293622254526	39.0	36.0	40.0	32.0	41.0
15	37.29777358394328	39.0	36.0	40.0	32.0	41.0
16	36.96621979659903	38.0	35.0	40.0	32.0	41.0
17	36.905239176081544	38.0	35.0	40.0	32.0	41.0
18	36.822031232732314	38.0	35.0	40.0	32.0	41.0
19	36.46249210113942	38.0	34.0	40.0	31.0	41.0
20	36.50244970940694	38.0	35.0	40.0	31.0	41.0
21	36.89853470823097	39.0	35.0	40.0	32.0	41.0
22	37.231689520231	39.0	35.0	40.0	32.0	41.0
23	37.51350669738367	39.0	36.0	40.0	33.0	41.0
24	37.5129427966642	39.0	36.0	41.0	33.0	41.0
25	36.75156064483039	38.0	35.0	40.0	31.0	41.0
26	36.960577955732376	39.0	35.0	40.0	32.0	41.0
27	37.19291071949765	39.0	35.0	40.0	32.0	41.0
28	37.092924604490236	39.0	35.0	40.0	31.0	41.0
29	37.170791076200274	39.0	35.0	41.0	31.0	41.0
30	36.81863082638375	39.0	35.0	40.0	31.0	41.0
31	36.88063723614972	39.0	35.0	40.0	31.0	41.0
32	36.79251287195486	39.0	35.0	40.0	31.0	41.0
33	36.78231448658115	39.0	35.0	41.0	31.0	41.0
34	36.739571378779765	40.0	35.0	41.0	31.0	41.0
35	36.71136500811847	40.0	35.0	41.0	30.0	41.0
36	36.543444441610774	40.0	35.0	41.0	30.0	41.0
37	36.53039254857622	40.0	35.0	41.0	30.0	41.0
38	36.3984794516278	39.0	35.0	41.0	30.0	41.0
39	36.38647885088935	39.0	35.0	41.0	30.0	41.0
40	36.26848475059436	39.0	35.0	40.0	29.0	41.0
41	36.034397943887626	39.0	35.0	40.0	28.0	41.0
42	36.1868920002607	39.0	35.0	41.0	29.0	41.0
43	36.225429938877696	39.0	35.0	41.0	30.0	41.0
44	36.37262786236288	39.0	35.0	41.0	31.0	41.0
45	36.37025891260672	39.0	35.0	41.0	30.0	41.0
46	36.22665125149122	39.0	35.0	40.0	30.0	41.0
47	35.94153851385241	39.0	35.0	40.0	28.0	41.0
48	36.12718653212392	39.0	35.0	40.0	30.0	41.0
49	36.10585181595867	39.0	35.0	40.0	30.0	41.0
50	35.92316498488236	39.0	35.0	40.0	29.0	41.0
51	35.068793054103296	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	7.0
11	20.0
12	10.0
13	9.0
14	15.0
15	27.0
16	48.0
17	72.0
18	167.0
19	383.0
20	749.0
21	1096.0
22	1595.0
23	2167.0
24	2818.0
25	3497.0
26	4306.0
27	4684.0
28	4841.0
29	5409.0
30	6712.0
31	8720.0
32	12114.0
33	18215.0
34	24582.0
35	29306.0
36	43306.0
37	69477.0
38	83317.0
39	25221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.16051618168371	17.697981575464937	19.312324489443156	16.829177753408196
2	30.115982193205422	32.170394362126274	20.0077075877234	17.705915856944905
3	27.762050898415698	30.870305668194014	25.445524073460113	15.922119359930178
4	25.791515419425952	21.06693416529942	35.435634558329724	17.705915856944905
5	31.361097651169317	23.836565136200445	24.686383356144393	20.11595385648585
6	22.408111102610096	36.715320814170624	26.043995590806436	14.832572492412844
7	66.02030609324481	6.485141641092778	21.936304721747582	5.558247543914831
8	64.17841932110888	14.13803949571974	14.660851971810631	7.022689211360758
9	57.99166333710212	9.23266997072817	15.276325520899746	17.49934117126997
10	34.4900382262347	27.60704904236056	22.60561803802221	15.297294693382527
11	29.86010161547638	23.16866865590438	27.99809577244481	18.97313395617443
12	26.402455093383658	19.183959149785064	31.63313015905401	22.78045559777727
13	24.554900977333457	21.36078594725403	35.57476785142491	18.5095452239876
14	18.81898220170644	29.142332508734793	29.31518649811986	22.72349879143891
15	16.61636898942757	25.505597918951317	38.37698604983296	19.501047041788162
16	18.341225109733948	26.32707941932394	30.398782654527217	24.932912816414895
17	18.468740347804896	27.002626813904264	32.54047191972774	21.9881609185631
18	17.719800849534852	26.482081275379077	32.61074698426462	23.187370890821455
19	19.541851917970867	26.941702866825917	29.61073848324875	23.905706731954467
20	21.54384115568477	26.862643419221932	34.29593169717114	17.297583727922152
21	20.915332715592847	29.684130586938473	31.39680191782918	18.003734779639498
22	18.760041824998087	23.596269754235628	32.645601149337345	24.998087271428936
23	20.472429788693084	28.214588309969706	32.23075157481319	19.08223032652402
24	21.198416544110355	25.015089303171727	29.87426997526204	23.912224177455872
25	18.691183596439775	32.61584759378746	27.87171400315671	20.821254806616057
26	19.941116296730794	25.50984842688701	31.29790676652527	23.251128509856926
27	23.51607683784879	27.447229943978307	27.623200972516216	21.41349224565669
28	17.786675507723174	27.905434699446584	32.63568329748738	21.67220649534286
29	24.5665190323577	24.39593198053834	29.332188529862652	21.70536045724131
30	22.081388725952753	28.461967871827348	29.643892445147195	19.8127509570727
31	21.44182896522801	28.267861342763794	26.408122437297926	23.88218725471027
32	24.10321366736658	32.32624631976855	24.96408320794335	18.60645680492152
33	21.618366728157348	25.33076035919626	26.141190538936073	26.909682373710325
34	23.742487227223652	25.934899220456842	29.614138889597307	20.708474662722196
35	23.97994893723133	27.25964086041615	26.181995415118774	22.57841478723374
36	25.22478102799951	29.193055236767464	25.652665493526477	19.92949824170655
37	21.553759007534733	29.303285075899904	30.539899517992403	18.603056398572964
38	20.228167265988287	29.098693960594957	25.28428813909929	25.388850634317468
39	22.484903612648377	27.084519933465383	29.820996942467957	20.60957951141828
40	19.66369981212755	26.86094321604765	30.685550256588996	22.789806715235805
41	21.93007064344189	27.0377643461727	28.916772220947067	22.11539278943834
42	20.244602563339654	28.160465175588484	31.25455158558114	20.34038067549072
43	26.19673050929586	24.840535110612386	26.245186299762825	22.717548080328935
44	22.97002825170941	23.728602234633705	29.264747137282903	24.036622376373977
45	20.53817097809855	23.645859013485445	28.63255492364671	27.183415084769297
46	25.136370462936984	28.004896585141925	26.335013700803913	20.523719251117175
47	18.70648542500829	24.90712640160499	35.35515827474717	21.031229898639552
48	21.19019889543467	25.60732674221236	28.611869118359646	24.590605243993323
49	19.793481987764206	23.503041946845983	34.69689627910535	22.006579786284462
50	21.43984539485802	22.830328224222797	30.590622246025067	25.139204134894122
51	20.640466535751024	22.024431919614393	28.588916375506873	28.74618516912771
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	156.0
1	291.0
2	426.0
3	2862.5
4	5299.0
5	3582.0
6	1865.0
7	1791.5
8	1718.0
9	1673.0
10	1628.0
11	1554.0
12	1480.0
13	1439.0
14	1398.0
15	1347.5
16	1297.0
17	1204.0
18	1111.0
19	1150.0
20	1189.0
21	1321.5
22	1454.0
23	1347.0
24	1240.0
25	1865.0
26	3271.5
27	4053.0
28	4988.0
29	5923.0
30	6176.5
31	6430.0
32	7102.0
33	7774.0
34	8957.5
35	10141.0
36	10221.5
37	10302.0
38	11443.5
39	12585.0
40	13785.0
41	14985.0
42	16044.5
43	17104.0
44	18963.5
45	20823.0
46	39433.0
47	58043.0
48	45169.5
49	32296.0
50	31822.5
51	31349.0
52	26743.5
53	22138.0
54	19587.0
55	17036.0
56	15760.0
57	14484.0
58	13110.5
59	11737.0
60	10998.0
61	10259.0
62	9426.0
63	8593.0
64	6779.0
65	4965.0
66	4038.5
67	3112.0
68	2541.0
69	1970.0
70	1631.5
71	1293.0
72	1130.5
73	968.0
74	807.5
75	557.0
76	467.0
77	378.0
78	289.0
79	226.0
80	163.0
81	145.0
82	127.0
83	103.0
84	79.0
85	43.5
86	8.0
87	5.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	352899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.06921967324231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.18793039521708	25.61594117411103
2	9.75729001228452	6.648465136881089
3	3.386372002194155	3.461131549142101
4	1.7573295518986627	2.3948338616766405
5	1.16877015394652	1.9909543561166607
6	0.9048275360821256	1.8496060855908314
7	0.7238066656325216	1.7261669804673994
8	0.6337373758976185	1.727275029968008
9	0.556185977055795	1.7053940008341706
>10	5.80117272845639	37.005281762728046
>50	0.07754909472190177	1.753971755293066
>100	0.03335444934275345	2.116186524972149
>500	0.0033354449342753454	0.636361634573448
>1k	0.005837028634981855	4.166748256378911
>5k	0.002501583700706509	7.201681891266476
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	8715	2.4695451106407216	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	8700	2.4652946027050233	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	7935	2.248518697984409	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3219	0.9121590030008586	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	2544	0.7208861458944343	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAACTAGT	2156	0.6109396739577045	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	2069	0.5862867279306544	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	1815	0.5143114602194963	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC	1450	0.41088243378417055	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTA	1414	0.40068121473849455	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	642	0.1819217396478879	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	554	0.15698542642512447	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATG	528	0.14961787933658072	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGAACTAG	516	0.14621747298802207	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC	468	0.13261584759378747	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT	430	0.12184789415668505	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGT	430	0.12184789415668505	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8336719571322105E-4	0.0	0.0	0.16066919996939635	0.0
2	2.8336719571322105E-4	0.0	0.0	1.2553166770095694	0.0
3	2.8336719571322105E-4	0.0	0.0	1.533016528808526	0.0
4	2.8336719571322105E-4	0.0	0.0	2.5049660101048743	0.0
5	2.8336719571322105E-4	0.0	0.0	6.042522081388726	0.0
6	2.8336719571322105E-4	0.0	0.0	6.763691594478874	0.0
7	2.8336719571322105E-4	0.0	0.0	7.840770305384827	0.0
8	2.8336719571322105E-4	0.0	0.0	9.002859175004746	0.0
9	2.8336719571322105E-4	0.0	0.0	9.356784802450559	0.0
10	2.8336719571322105E-4	0.0	0.0	12.623158467436859	0.0
11	2.8336719571322105E-4	0.0	0.0	13.800549165625291	0.0
12	2.8336719571322105E-4	0.0	0.0	17.055588142783062	0.0
13	2.8336719571322105E-4	0.0	0.0	17.654059660129384	0.0
14	2.8336719571322105E-4	0.0	0.0	17.927792371188357	0.0
15	2.8336719571322105E-4	0.0	0.0	18.667380751999865	0.0
16	2.8336719571322105E-4	0.0	0.0	19.35511293599585	0.0
17	2.8336719571322105E-4	0.0	0.0	20.08506683215311	0.0
18	2.8336719571322105E-4	0.0	0.0	20.75636371879773	0.0
19	2.8336719571322105E-4	0.0	0.0	21.912785244503386	0.0
20	2.8336719571322105E-4	0.0	0.0	22.590316209453697	0.0
21	2.8336719571322105E-4	0.0	0.0	23.1862374220386	0.0
22	2.8336719571322105E-4	0.0	0.0	23.937160490678636	0.0
23	2.8336719571322105E-4	0.0	0.0	24.521180281043584	0.0
24	2.8336719571322105E-4	0.0	0.0	24.978534934924724	0.0
25	2.8336719571322105E-4	0.0	0.0	25.428238674521605	0.0
26	2.8336719571322105E-4	0.0	0.0	25.838837741110062	0.0
27	2.8336719571322105E-4	0.0	0.0	26.33104656006393	0.0
28	2.8336719571322105E-4	0.0	0.0	26.74362919702238	0.0
29	2.8336719571322105E-4	0.0	0.0	27.193049569423547	0.0
30	2.8336719571322105E-4	0.0	0.0	27.693192669857382	0.0
31	2.8336719571322105E-4	0.0	0.0	28.18058424648412	0.0
32	2.8336719571322105E-4	0.0	0.0	28.635671962799556	0.0
33	2.8336719571322105E-4	0.0	0.0	29.073190912980767	0.0
34	2.8336719571322105E-4	0.0	0.0	29.510426495966268	0.0
35	2.8336719571322105E-4	0.0	0.0	30.020487448250066	0.0
36	2.8336719571322105E-4	0.0	0.0	30.513829735986782	0.0
37	2.8336719571322105E-4	0.0	0.0	30.98676958563215	0.0
38	2.8336719571322105E-4	0.0	0.0	31.43959036438188	0.0
39	2.8336719571322105E-4	0.0	0.0	31.901478893394426	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	25	3.885293E-5	45.0	4
ATTTACG	25	3.885293E-5	45.0	35
CGGGTGA	25	3.885293E-5	45.0	6
TTTACGC	25	3.885293E-5	45.0	36
CGTTGAT	20	7.026635E-4	45.0	25
ATAGCGG	25	3.885293E-5	45.0	2
TCTAGTA	55	1.8189894E-12	45.0	19
TTCCCGT	20	7.026635E-4	45.0	38
TCGTGTA	25	3.885293E-5	45.0	17
CGAATAC	20	7.026635E-4	45.0	45
TGCTCGG	40	6.7921064E-9	45.0	2
CGTAAGG	20	7.026635E-4	45.0	2
TTACGCA	25	3.885293E-5	45.0	37
TAAGGGC	45	3.8380676E-10	45.0	4
GCTAACG	45	3.8380676E-10	45.0	1
TATCGTG	25	3.885293E-5	45.0	1
AGTACGG	20	7.026635E-4	45.0	2
AGTCGAA	35	1.2087366E-7	45.0	42
GTGCTCG	30	2.1612104E-6	44.999996	1
ATCGCGG	30	2.1612104E-6	44.999996	2
>>END_MODULE