FastQCFastQC Report
Sat 18 Jun 2016
SRR3551868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353352
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCG95552.7041024247775587No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC93512.64636962575562No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGC90732.5676945368923905No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36791.0411714098123117No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC31040.8784441576671421No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCGTAAGT27100.7669406144581041No Hit
GAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT21240.6011003192284181No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT20750.5872331273064819No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTA19010.5379904457877697No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTC15150.42875093391292535No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC7710.21819601983291448No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT7150.20234780049355883No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC6640.18791460073807423No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATG5530.1565011659761371No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCGTAAG5350.15140709547420136No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGT5200.14716203672258824No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTAT4260.12055966854581267No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT3960.11206955104258642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG351.2087366E-745.0000042
CGAAAGG351.2087366E-745.0000042
CGAATAT700.045.00000414
GCGGCTA351.2087366E-745.00000424
TAACGCC207.026649E-445.012
TTATCGG207.026649E-445.02
TACATGA207.026649E-445.038
TCTCGAG207.026649E-445.01
CATCGTT207.026649E-445.036
ACGTGCG253.885305E-544.9999961
CCGTTGA253.885305E-544.99999616
CTAGCGG302.1612195E-644.9999962
CGTTTTT19100.043.821991
TACGGCT10350.043.260877
ACGGCTG10700.042.2663548
TACGAAT750.042.00000412
GCTACGA750.042.00000410
TAGCGGG650.041.5384643
GACCGAT650.041.5384649
TGATACC11150.041.3677144