##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551868_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	353352
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.695066109714958	30.0	25.0	31.0	16.0	33.0
2	27.864398673277638	30.0	25.0	33.0	16.0	33.0
3	27.76338608526342	30.0	25.0	33.0	16.0	34.0
4	31.72439380561027	35.0	28.0	35.0	19.0	37.0
5	33.348270280060675	35.0	32.0	35.0	28.0	37.0
6	33.27022062985352	35.0	33.0	35.0	28.0	37.0
7	33.83522096945822	35.0	33.0	36.0	30.0	37.0
8	33.516827412891395	35.0	33.0	37.0	28.0	37.0
9	35.73445176481243	38.0	35.0	39.0	28.0	39.0
10	35.33143154701261	37.0	34.0	39.0	31.0	39.0
11	36.10327944938758	37.0	35.0	39.0	32.0	39.0
12	36.14235380017659	37.0	35.0	39.0	32.0	39.0
13	36.20748432158301	37.0	35.0	39.0	32.0	39.0
14	37.249569834046504	39.0	36.0	40.0	32.0	41.0
15	37.32123208585207	39.0	36.0	40.0	32.0	41.0
16	36.89986755416695	38.0	35.0	40.0	32.0	41.0
17	36.863125155652156	38.0	35.0	40.0	32.0	41.0
18	36.817674726618215	38.0	35.0	40.0	32.0	41.0
19	36.45678813194775	38.0	34.0	40.0	31.0	41.0
20	36.42396533768027	38.0	35.0	40.0	31.0	41.0
21	36.83180794222192	38.0	35.0	40.0	32.0	41.0
22	37.1864995811542	39.0	35.0	40.0	32.0	41.0
23	37.42866320269872	39.0	35.0	40.0	32.0	41.0
24	37.46054925400168	39.0	35.0	41.0	33.0	41.0
25	36.70923611582784	38.0	35.0	40.0	31.0	41.0
26	36.91724682469605	39.0	35.0	40.0	32.0	41.0
27	37.13478344540288	39.0	35.0	40.0	32.0	41.0
28	37.03181247028459	39.0	35.0	40.0	31.0	41.0
29	37.16771944123707	39.0	35.0	41.0	32.0	41.0
30	36.69982906563427	39.0	35.0	40.0	31.0	41.0
31	36.992262672915395	39.0	35.0	40.0	32.0	41.0
32	36.846190201272385	39.0	35.0	40.0	31.0	41.0
33	36.68340068826553	39.0	35.0	41.0	30.0	41.0
34	36.56327401571238	39.0	35.0	41.0	30.0	41.0
35	36.43905227648351	39.0	35.0	41.0	30.0	41.0
36	36.30226516334986	39.0	35.0	41.0	30.0	41.0
37	36.24041465733886	39.0	35.0	41.0	30.0	41.0
38	36.262452172338065	39.0	35.0	41.0	30.0	41.0
39	36.17512848377822	39.0	35.0	41.0	30.0	41.0
40	35.92023534605719	39.0	35.0	40.0	27.0	41.0
41	35.747198261223936	39.0	35.0	40.0	26.0	41.0
42	35.972876904616356	39.0	35.0	40.0	28.0	41.0
43	35.966011229595416	39.0	35.0	40.0	28.0	41.0
44	36.13392594353506	39.0	35.0	40.0	29.0	41.0
45	36.19476612556318	39.0	35.0	40.0	30.0	41.0
46	36.01375682039439	39.0	35.0	40.0	29.0	41.0
47	35.74857366025946	39.0	35.0	40.0	26.0	41.0
48	35.90091466865901	39.0	35.0	40.0	29.0	41.0
49	35.93460628495098	39.0	35.0	40.0	29.0	41.0
50	35.742650388281376	39.0	35.0	40.0	28.0	41.0
51	34.805581969254455	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	11.0
10	8.0
11	9.0
12	10.0
13	15.0
14	20.0
15	17.0
16	40.0
17	65.0
18	166.0
19	416.0
20	777.0
21	1136.0
22	1628.0
23	2168.0
24	2988.0
25	4118.0
26	4699.0
27	5050.0
28	5235.0
29	5642.0
30	6917.0
31	9019.0
32	12559.0
33	18253.0
34	24147.0
35	29530.0
36	43218.0
37	69445.0
38	81736.0
39	24309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.581448527247616	16.09924381353438	19.088897190337114	17.230410468880887
2	30.967703593017724	32.44809708166361	19.781690778600375	16.802508546718286
3	26.55425751092395	30.76535579252417	26.41700061128846	16.26338608526342
4	25.293475061694853	20.011206955104257	37.42245692680387	17.27286105639702
5	31.338155720075168	24.09127442323802	25.396771491317438	19.173798365369375
6	21.42169847630691	37.68848060857162	27.015270891349136	13.874550023772327
7	63.14100387149358	6.5671058887455	24.019391428377368	6.272498811383549
8	60.50114333582377	15.609081029681452	15.97896714890534	7.910808485589441
9	55.18802780230479	8.821798093685617	17.008252394213137	18.981921709796463
10	34.77410627363083	23.701012022006385	25.39733749915099	16.127544205211798
11	29.4505195951912	22.723233489551493	28.50302248183115	19.32322443342616
12	24.93830514614322	18.50958817270031	32.82930335755847	23.722803323598
13	24.097217505490278	21.32151508976884	37.29595417600579	17.285313228735085
14	19.091444225588084	28.5287758382576	29.449670583440874	22.93010935271344
15	16.506769453689238	24.24692657746383	39.13066856845299	20.11563540039394
16	17.72396929973511	25.642984898911003	30.20812108039575	26.42492472095814
17	18.34686092055514	26.9564060766601	33.26512938939075	21.43160361339401
18	17.37049740768412	25.538556453621318	34.00659965133917	23.084346487355383
19	18.521191333288055	27.04357128302656	30.32047363535511	24.114763748330276
20	20.582025855237838	26.15890103919038	37.03502456473998	16.224048540831806
21	20.45863614752428	30.17302859471575	32.05641966084811	17.311915596911863
22	18.054234870610607	23.080101428603772	33.94631981706627	24.91934388371935
23	19.10814145667776	28.421800357716954	32.63374765106749	19.836310534537798
24	20.939742806040435	24.34484593266771	30.627532885055132	24.087878376236727
25	16.88939074916797	33.11428830175009	28.511229595417603	21.485091353664334
26	18.606658516153864	24.331827752496093	33.22465982929204	23.836853902058007
27	21.90110711132242	26.77811360909235	29.605888745500238	21.71489053408499
28	16.223482532998258	25.87702913808327	34.04282415268627	23.856664176232197
29	24.612850641852884	21.556691344608208	31.321741492902262	22.508716520636646
30	18.226584255926102	27.767778306051756	34.45770789467726	19.547929543344882
31	21.596594896873373	24.88708143720709	26.435961873712333	27.080361792207203
32	25.711471846770362	27.61750322624465	27.57109058389368	19.099934343091306
33	20.194876497090718	23.767234938531548	30.584799293622222	25.45308927075551
34	23.980902895696076	27.43722973125948	27.32883923113496	21.253028141909482
35	21.039360184744957	26.108809345921348	30.29245624759447	22.55937422173923
36	25.007924109669677	24.767370780411603	27.333933301636893	22.890771808281826
37	21.448017840566912	25.136973895718718	34.18998618940886	19.22502207430551
38	20.31515316171976	24.851422943693542	28.531888881342116	26.30153501324458
39	26.003531888881344	26.071452828907155	27.953711879372413	19.971303402839094
40	19.579908985940364	26.5460503973375	33.33644637641785	20.537594240304287
41	21.763567207770155	29.690789920532502	26.286252801738776	22.259390069958567
42	20.689567343612037	25.838540605401978	32.41272159206684	21.05917045891915
43	26.692646426226542	22.480416128959224	27.46156806810206	23.365369376712174
44	21.542824152686276	22.868414498856666	31.553238696823566	24.0355226516335
45	20.179877289501686	22.481265140709546	29.44316149335507	27.895696076433694
46	27.127340442391723	25.90872557676198	26.90971043039236	20.05422355045394
47	18.288279109782877	23.705257080757995	38.009124046276796	19.997339763182325
48	20.894745183273336	25.424222871244538	29.140064298489893	24.540967646992236
49	20.260533405782336	22.043175077543072	35.61858996128506	22.077701555389524
50	22.188073082931467	21.148033688786253	31.434377051778394	25.229516176503886
51	20.238176096357176	21.291233670674	29.452500622608618	29.01808961036021
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	131.0
1	311.0
2	491.0
3	3244.5
4	5998.0
5	4031.0
6	2064.0
7	2041.0
8	2018.0
9	1937.5
10	1857.0
11	1822.5
12	1788.0
13	1706.0
14	1624.0
15	1552.0
16	1480.0
17	1422.0
18	1364.0
19	1341.0
20	1318.0
21	1395.5
22	1473.0
23	1424.0
24	1375.0
25	1940.5
26	3243.5
27	3981.0
28	4301.5
29	4622.0
30	5297.5
31	5973.0
32	6604.5
33	7236.0
34	7871.5
35	8507.0
36	9668.5
37	10830.0
38	11794.0
39	12758.0
40	13434.0
41	14110.0
42	15774.5
43	17439.0
44	18702.5
45	19966.0
46	40584.5
47	61203.0
48	48206.5
49	35210.0
50	33720.0
51	32230.0
52	27407.5
53	22585.0
54	20048.0
55	17511.0
56	15281.0
57	13051.0
58	11980.0
59	10909.0
60	9964.5
61	9020.0
62	7924.0
63	6828.0
64	5716.0
65	4604.0
66	3733.5
67	2863.0
68	2362.5
69	1862.0
70	1648.0
71	1434.0
72	1250.5
73	1067.0
74	898.0
75	597.5
76	466.0
77	435.0
78	404.0
79	345.5
80	287.0
81	193.5
82	100.0
83	79.0
84	58.0
85	37.0
86	16.0
87	9.5
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	353352.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.02697130243808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.55873827937143	26.465937573599724
2	10.080386278343926	7.061708017780864
3	3.5670528300244677	3.7482917133464264
4	1.7682329322481154	2.4774337669552264
5	1.1449137589034875	2.0051430688439478
6	0.8419319340538097	1.7694195415625387
7	0.6449223280057773	1.581277311275194
8	0.5565892415384345	1.5596508312490014
9	0.5152657051325275	1.6243377360126803
>10	5.210974265771147	34.19158698827474
>50	0.06886392672689558	1.669453367240516
>100	0.029165898378449892	1.984728344463738
>500	0.004860983063074982	1.0664296709314738
>1k	0.005671146906920812	4.854837363037693
>5k	0.002430491531537491	7.939764705426211
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCG	9555	2.7041024247775587	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC	9351	2.64636962575562	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGC	9073	2.5676945368923905	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3679	1.0411714098123117	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	3104	0.8784441576671421	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCGTAAGT	2710	0.7669406144581041	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT	2124	0.6011003192284181	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT	2075	0.5872331273064819	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTA	1901	0.5379904457877697	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTC	1515	0.42875093391292535	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	771	0.21819601983291448	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT	715	0.20234780049355883	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC	664	0.18791460073807423	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATG	553	0.1565011659761371	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCGTAAG	535	0.15140709547420136	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGT	520	0.14716203672258824	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTAT	426	0.12055966854581267	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT	396	0.11206955104258642	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21083791799678508	0.0
2	0.0	0.0	0.0	1.5177500056600783	0.0
3	0.0	0.0	0.0	1.8222622201091263	0.0
4	0.0	0.0	0.0	2.8639996377549863	0.0
5	0.0	0.0	0.0	6.595123276506147	0.0
6	0.0	0.0	0.0	7.321877334782314	0.0
7	0.0	0.0	0.0	8.466345174217212	0.0
8	0.0	0.0	0.0	9.694016165183726	0.0
9	0.0	0.0	0.0	10.024281736059226	0.0
10	0.0	0.0	0.0	13.641920804183929	0.0
11	0.0	0.0	0.0	14.98958545586271	0.0
12	0.0	0.0	0.0	18.719577078946774	0.0
13	0.0	0.0	0.0	19.334827594013902	0.0
14	0.0	0.0	0.0	19.672734270642305	0.0
15	0.0	0.0	0.0	20.61994838008558	0.0
16	0.0	0.0	0.0	21.430471597726914	0.0
17	0.0	0.0	0.0	22.304953700559217	0.0
18	0.0	0.0	0.0	23.096232651859903	0.0
19	0.0	0.0	0.0	24.470499671715455	0.0
20	0.0	0.0	0.0	25.183103534152913	0.0
21	0.0	0.0	0.0	25.87504811066585	0.0
22	0.0	0.0	0.0	26.66434603454912	0.0
23	0.0	0.0	0.0	27.323745160633024	0.0
24	0.0	0.0	0.0	27.887205958930473	0.0
25	0.0	0.0	0.0	28.38868889945437	0.0
26	2.8300391677420813E-4	0.0	0.0	28.912246145486655	0.0
27	2.8300391677420813E-4	0.0	0.0	29.452500622608618	0.0
28	2.8300391677420813E-4	0.0	0.0	29.947191469129933	0.0
29	2.8300391677420813E-4	0.0	0.0	30.44669338223641	0.0
30	2.8300391677420813E-4	0.0	0.0	30.968835608684824	0.0
31	2.8300391677420813E-4	0.0	0.0	31.496071905635173	0.0
32	2.8300391677420813E-4	0.0	0.0	32.004629944078424	0.0
33	2.8300391677420813E-4	0.0	0.0	32.47951051642555	0.0
34	2.8300391677420813E-4	0.0	0.0	32.960051167108155	0.0
35	2.8300391677420813E-4	0.0	0.0	33.4686092055514	0.0
36	2.8300391677420813E-4	0.0	0.0	33.99301546333401	0.0
37	2.8300391677420813E-4	0.0	0.0	34.50383753311146	0.0
38	2.8300391677420813E-4	0.0	0.0	34.98041612895923	0.0
39	5.660078335484163E-4	0.0	0.0	35.50793542982635	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	35	1.2087366E-7	45.000004	2
CGAAAGG	35	1.2087366E-7	45.000004	2
CGAATAT	70	0.0	45.000004	14
GCGGCTA	35	1.2087366E-7	45.000004	24
TAACGCC	20	7.026649E-4	45.0	12
TTATCGG	20	7.026649E-4	45.0	2
TACATGA	20	7.026649E-4	45.0	38
TCTCGAG	20	7.026649E-4	45.0	1
CATCGTT	20	7.026649E-4	45.0	36
ACGTGCG	25	3.885305E-5	44.999996	1
CCGTTGA	25	3.885305E-5	44.999996	16
CTAGCGG	30	2.1612195E-6	44.999996	2
CGTTTTT	1910	0.0	43.82199	1
TACGGCT	1035	0.0	43.26087	7
ACGGCTG	1070	0.0	42.266354	8
TACGAAT	75	0.0	42.000004	12
GCTACGA	75	0.0	42.000004	10
TAGCGGG	65	0.0	41.538464	3
GACCGAT	65	0.0	41.538464	9
TGATACC	1115	0.0	41.367714	4
>>END_MODULE