SRR3551867_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551867_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	443903
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	12674	2.8551282600027483	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	12095	2.7246943589027333	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC	10558	2.3784475437201373	No Hit
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	3743	0.8432022311180596	Illumina PCR Primer Index 10 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3415	0.7693122146054431	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCATTAGCT	2895	0.6521695055000755	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCT	2653	0.597653090877962	No Hit
GAACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT	2558	0.5762520190221738	Illumina PCR Primer Index 10 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTA	1908	0.42982363264046425	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTC	1886	0.42486759494754484	No Hit
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	927	0.2088294064243765	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	720	0.1621975972228167	Illumina PCR Primer Index 10 (95% over 24bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCATTAGC	653	0.14710420970347127	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTT	625	0.14079652536702839	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATG	618	0.13921960428291766	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGT	548	0.12345039344181048	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	532	0.11984600239241455	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAG	55	1.8189894E-12	45.000004	26
TAGCGAC	20	7.0286036E-4	45.0	1
ACGATAG	20	7.0286036E-4	45.0	1
TACGCGG	20	7.0286036E-4	45.0	2
CATAGTC	20	7.0286036E-4	45.0	30
CCATAAG	25	3.8869235E-5	45.0	43
TTAAACG	20	7.0286036E-4	45.0	26
ATCGGAA	20	7.0286036E-4	45.0	38
CGCAACC	25	3.8869235E-5	45.0	12
GCTAAGT	20	7.0286036E-4	45.0	11
TACGTCC	20	7.0286036E-4	45.0	28
GATACCC	30	2.16248E-6	44.999996	35
TACGGCT	1005	0.0	43.880596	7
CGTTTTT	1985	0.0	43.753147	1
CCGATGA	665	0.0	43.308273	18
CGGCGAT	105	0.0	42.857143	31
GGCGACT	270	0.0	42.5	10
ACGGCTG	1050	0.0	41.999996	8
TGATACC	1405	0.0	41.63701	4
GAATGAC	325	0.0	41.538464	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content