##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551866_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	373080
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.619783960544655	28.0	25.0	31.0	16.0	33.0
2	27.854521818376757	31.0	25.0	33.0	16.0	34.0
3	27.696786212072478	31.0	25.0	33.0	16.0	34.0
4	31.694065615953683	35.0	28.0	35.0	19.0	37.0
5	33.331682212930204	35.0	32.0	35.0	28.0	37.0
6	33.1798917122333	35.0	32.0	35.0	28.0	37.0
7	34.01120671169722	35.0	33.0	37.0	31.0	37.0
8	34.06145062721132	35.0	35.0	37.0	31.0	37.0
9	36.371043743969125	39.0	35.0	39.0	32.0	39.0
10	35.510957435402595	37.0	34.0	39.0	31.0	39.0
11	36.10352203280798	37.0	35.0	39.0	32.0	39.0
12	36.17879811300525	37.0	35.0	39.0	32.0	39.0
13	36.28103087809585	37.0	35.0	39.0	32.0	39.0
14	37.31965262142168	39.0	36.0	40.0	32.0	41.0
15	37.38868607269218	39.0	36.0	40.0	32.0	41.0
16	37.04994371180444	38.0	36.0	40.0	32.0	41.0
17	37.03329848825989	38.0	36.0	40.0	32.0	41.0
18	36.97016993674279	38.0	36.0	40.0	32.0	41.0
19	36.626782459526105	38.0	35.0	40.0	31.0	41.0
20	36.66593492012437	38.0	35.0	40.0	31.0	41.0
21	36.99672188270612	39.0	35.0	40.0	32.0	41.0
22	37.31275865766055	39.0	36.0	40.0	32.0	41.0
23	37.61596976519781	39.0	36.0	40.0	33.0	41.0
24	37.64914763589579	39.0	36.0	41.0	33.0	41.0
25	36.844711590007506	39.0	35.0	40.0	31.0	41.0
26	37.03698670526428	39.0	35.0	40.0	32.0	41.0
27	37.29779403881205	39.0	35.0	40.0	33.0	41.0
28	37.296695078803474	39.0	35.0	40.0	32.0	41.0
29	37.40190039669776	39.0	36.0	41.0	33.0	41.0
30	37.00258657660555	39.0	35.0	40.0	31.0	41.0
31	37.089766269968905	39.0	35.0	40.0	32.0	41.0
32	37.033539723383726	39.0	36.0	40.0	32.0	41.0
33	37.00309585075587	39.0	36.0	41.0	31.0	41.0
34	36.8869250562882	40.0	36.0	41.0	31.0	41.0
35	36.796920231585716	40.0	36.0	41.0	31.0	41.0
36	36.649241449555056	40.0	35.0	41.0	30.0	41.0
37	36.68175994424788	40.0	36.0	41.0	31.0	41.0
38	36.51854025946178	39.0	35.0	41.0	30.0	41.0
39	36.44911279082234	39.0	35.0	41.0	30.0	41.0
40	36.2991771201887	39.0	35.0	41.0	30.0	41.0
41	36.210724241449554	39.0	35.0	41.0	29.0	41.0
42	36.37511793717165	39.0	35.0	41.0	30.0	41.0
43	36.374941031414174	39.0	35.0	41.0	30.0	41.0
44	36.50498820628283	39.0	35.0	41.0	31.0	41.0
45	36.533847968264176	39.0	35.0	41.0	31.0	41.0
46	36.35347378578321	39.0	35.0	41.0	30.0	41.0
47	36.22609896000858	39.0	35.0	41.0	30.0	41.0
48	36.32058003645331	39.0	35.0	41.0	30.0	41.0
49	36.34324273614238	39.0	35.0	41.0	30.0	41.0
50	36.19077141631821	39.0	35.0	41.0	30.0	41.0
51	35.333389621528895	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	11.0
11	9.0
12	17.0
13	14.0
14	13.0
15	32.0
16	38.0
17	66.0
18	174.0
19	402.0
20	727.0
21	1061.0
22	1546.0
23	2003.0
24	2829.0
25	3801.0
26	4541.0
27	4699.0
28	4928.0
29	5226.0
30	6491.0
31	8600.0
32	12204.0
33	17931.0
34	24590.0
35	30311.0
36	44557.0
37	73403.0
38	93099.0
39	29751.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57467567277796	17.56057681998499	20.229441406668812	16.635306100568243
2	30.443604588828133	29.987938243808298	22.336764232872305	17.23169293449126
3	28.91899860619706	28.189396376112363	27.342661091454918	15.548943926235658
4	26.120939208748794	21.757531896644153	34.325613809370644	17.79591508523641
5	29.344644580250883	26.141310174761443	25.604159965691004	18.909885279296663
6	21.98590114720703	36.876005146349314	27.077570494263963	14.060523212179692
7	70.63418033665701	5.526964726064115	19.01549265573067	4.823362281548194
8	70.75345770344163	11.343411600729066	12.462742575318966	5.440388120510346
9	65.40581108609413	7.673957328186984	13.03607805296451	13.88415353275437
10	37.14833279725528	26.14640291626461	21.921035702798328	14.784228583681783
11	28.63058861370216	23.14329366355741	29.61643615310389	18.60968156963654
12	24.971051785139917	19.91342339444623	32.37402165755334	22.741503162860514
13	24.53870483542404	21.104052750080413	35.80652943068511	18.550712983810445
14	20.518655516243165	27.418784174975876	30.32807976841428	21.734480540366675
15	18.254797898574033	24.961134341160076	37.015653479146565	19.76841428111933
16	20.388120510346305	25.475233193953038	30.09649404953361	24.040152246167043
17	20.08657660555377	26.265948322075694	32.65331832314785	20.994156749222686
18	19.670043958400342	25.79259140130803	32.308352096065185	22.22901254422644
19	20.873003109252707	27.310496408277046	29.909938887101962	21.906561595368284
20	22.71871984560952	26.502090704406562	34.06159536828562	16.717594081698294
21	22.533504878310282	28.970730138308138	30.92580679746971	17.56995818591187
22	20.277420392409134	23.075479789857404	33.26069475715664	23.38640506057682
23	21.135145277152354	27.49597941460277	32.00519995711375	19.363675351131125
24	21.67551195454058	25.19995711375576	30.105875415460492	23.018655516243165
25	19.1420070762303	30.783210035381153	28.89755548407848	21.177227404310067
26	19.31301597512598	25.628015439047925	32.843089953897284	22.21587863192881
27	23.004717486866088	26.88592259032915	29.179532539937814	20.929827382866943
28	17.694328294199636	27.05532325506594	34.189181944891175	21.06116650584325
29	22.27565133483435	23.69572209713734	32.61713305457275	21.41149351345556
30	22.618741288731638	27.796987241342343	30.491047496515495	19.093223973410527
31	22.320145813230408	26.881902004931916	27.551731532111074	23.246220649726602
32	25.53366570172617	28.06180979950681	27.54261820521068	18.86190629355634
33	24.249222686823202	24.670311997426825	27.47266001929881	23.60780529645116
34	21.95909724455881	25.029484292913047	29.92307279939959	23.08834566312855
35	23.545352203280796	25.964404417283156	27.8594403345127	22.630803044923343
36	20.75721024981237	28.384528787391446	30.890961724027015	19.967299238769165
37	24.242789750187626	25.64034523426611	29.77565133483435	20.34121368071191
38	23.12962367320682	28.025088452878737	26.38495764983382	22.46033022408063
39	24.733301168650154	24.426396483327974	28.09075801436689	22.74954433365498
40	23.31832314785033	25.50498552589257	29.60946713841535	21.56722418784175
41	19.18355312533505	26.403720381687574	30.08094778599764	24.331778706979737
42	20.536614131017476	27.137075158143027	31.306690254101	21.0196204567385
43	22.38929988206283	25.020639005039136	30.353543475930095	22.236517636967942
44	23.030717272434867	23.908277045137773	29.56390050391337	23.49710517851399
45	20.91883778278117	23.623083520960652	28.881473142489543	26.57660555376863
46	24.730620778385333	26.514420499624748	27.85488367106251	20.900075050927416
47	20.56288195561274	24.16934705693149	34.38351023909082	20.884260748364962
48	21.48413208963225	25.506057681998502	28.72145384367964	24.28835638468961
49	20.576015867910368	23.634609199099387	33.174386190629356	22.614988742360886
50	22.086147743111397	22.77313176798542	30.69529323469497	24.445427254208212
51	21.232979521818375	22.369733033129624	29.139862764018442	27.25742468103356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	58.0
1	216.5
2	375.0
3	2830.0
4	5285.0
5	3623.0
6	1961.0
7	1908.5
8	1856.0
9	1792.5
10	1729.0
11	1697.0
12	1665.0
13	1589.5
14	1514.0
15	1470.0
16	1426.0
17	1402.5
18	1379.0
19	1306.0
20	1233.0
21	1375.5
22	1518.0
23	1456.5
24	1395.0
25	1936.0
26	2947.0
27	3417.0
28	4127.5
29	4838.0
30	5441.0
31	6044.0
32	7084.0
33	8124.0
34	9394.5
35	10665.0
36	11237.0
37	11809.0
38	12895.5
39	13982.0
40	15335.0
41	16688.0
42	17730.0
43	18772.0
44	20731.5
45	22691.0
46	38035.0
47	53379.0
48	43957.5
49	34536.0
50	33693.5
51	32851.0
52	29025.5
53	25200.0
54	22711.0
55	20222.0
56	18191.0
57	16160.0
58	15039.0
59	13918.0
60	12897.5
61	11877.0
62	10241.0
63	8605.0
64	7205.0
65	5805.0
66	4609.0
67	3413.0
68	2755.5
69	2098.0
70	1739.0
71	1380.0
72	1257.0
73	1134.0
74	880.5
75	503.5
76	380.0
77	336.0
78	292.0
79	238.0
80	184.0
81	128.0
82	72.0
83	47.0
84	22.0
85	16.5
86	11.0
87	8.5
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	373080.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.99818158175945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.0932084890665	28.534053717244685
2	9.04063574264056	6.870554371268013
3	3.3060123038194904	3.768673674961915
4	1.961867286724928	2.9818955760115005
5	1.460473783128397	2.7747674003355995
6	1.1341720242780038	2.5857884714080357
7	0.9744157238815961	2.5918217928522123
8	0.8259166020396138	2.5106663212552793
9	0.7061541233608727	2.4149315343757136
>10	5.432056036004338	33.38195609354505
>50	0.04103366666556571	1.054076154320217
>100	0.015564494252455957	1.2586545663165665
>500	0.002122431034425812	0.533086716148174
>1k	0.004244862068851624	3.0227065236359403
>5k	0.002122431034425812	5.716367086321117
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCG	7274	1.9497158786319289	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTC	7028	1.883778278117294	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGC	6962	1.866087702369465	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3022	0.8100139380293772	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	2190	0.5870054679961403	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGGCATT	1983	0.5315213895143133	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCT	1606	0.43047067653050286	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCT	1377	0.36908973946606627	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTA	1066	0.2857296022300847	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTC	957	0.2565133483435188	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	525	0.14072048890318428	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT	501	0.13428755226761016	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGAGGCAT	462	0.12383403023480219	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTT	412	0.1104320789106894	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1388442157178085	0.0
2	0.0	0.0	0.0	0.9922804760373111	0.0
3	0.0	0.0	0.0	1.2190414924412996	0.0
4	0.0	0.0	0.0	1.8853865122761875	0.0
5	0.0	0.0	0.0	4.489117615524821	0.0
6	0.0	0.0	0.0	5.102122869089739	0.0
7	0.0	0.0	0.0	5.887209177656267	0.0
8	0.0	0.0	0.0	6.795861477431114	0.0
9	0.0	0.0	0.0	7.092848718773453	0.0
10	0.0	0.0	0.0	9.755012329795218	0.0
11	0.0	0.0	0.0	10.79125120617562	0.0
12	0.0	0.0	0.0	13.549640827704513	0.0
13	0.0	0.0	0.0	14.013348343518816	0.0
14	0.0	0.0	0.0	14.256459740538222	0.0
15	0.0	0.0	0.0	14.814785032700762	0.0
16	0.0	0.0	0.0	15.39696579822022	0.0
17	0.0	0.0	0.0	16.080197276723492	0.0
18	0.0	0.0	0.0	16.74198563310818	0.0
19	0.0	0.0	0.0	17.762946284979094	0.0
20	0.0	0.0	0.0	18.322611772274044	0.0
21	0.0	0.0	0.0	18.844751795861477	0.0
22	0.0	0.0	0.0	19.499303098531147	0.0
23	0.0	0.0	0.0	20.107483649619386	0.0
24	0.0	0.0	0.0	20.588881741181517	0.0
25	0.0	0.0	0.0	21.007826739573282	0.0
26	0.0	0.0	0.0	21.400235874343306	0.0
27	2.680390264822558E-4	0.0	0.0	21.843572424144956	0.0
28	2.680390264822558E-4	0.0	0.0	22.256352524927628	0.0
29	2.680390264822558E-4	0.0	0.0	22.720328079768414	0.0
30	2.680390264822558E-4	0.0	0.0	23.212179693363353	0.0
31	2.680390264822558E-4	0.0	0.0	23.68928916050177	0.0
32	2.680390264822558E-4	0.0	0.0	24.137986490833065	0.0
33	2.680390264822558E-4	0.0	0.0	24.60678674815053	0.0
34	2.680390264822558E-4	0.0	0.0	25.053071727243488	0.0
35	2.680390264822558E-4	0.0	0.0	25.522408062613916	0.0
36	2.680390264822558E-4	0.0	0.0	26.02310496408277	0.0
37	2.680390264822558E-4	0.0	0.0	26.502626782459526	0.0
38	2.680390264822558E-4	0.0	0.0	27.003591722954862	0.0
39	2.680390264822558E-4	0.0	0.0	27.499195882920553	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCAC	30	2.1615451E-6	45.000004	34
CGAAATA	20	7.027155E-4	45.0	12
CGACGGT	20	7.027155E-4	45.0	28
CCGATCG	20	7.027155E-4	45.0	40
CCCCGAC	25	3.885723E-5	45.0	38
TGCGAAG	25	3.885723E-5	45.0	1
CGGGCTA	20	7.027155E-4	45.0	6
CGTACTA	20	7.027155E-4	45.0	29
TACTCGC	20	7.027155E-4	45.0	45
ATGCGCG	25	3.885723E-5	45.0	1
GTAAGCG	25	3.885723E-5	45.0	1
CGATCGA	20	7.027155E-4	45.0	41
TTACACG	25	3.885723E-5	45.0	34
TCGAATA	20	7.027155E-4	45.0	44
ACGACTA	20	7.027155E-4	45.0	12
TACGTAG	20	7.027155E-4	45.0	1
CGTTTTT	1790	0.0	42.486034	1
ACGGCTG	760	0.0	42.039474	8
TACGGCT	760	0.0	41.74342	7
CCGATGA	520	0.0	41.53846	18
>>END_MODULE