SRR3551865_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551865_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329060
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG	8933	2.7147024858688384	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC	8802	2.67489211693916	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC	8020	2.437245487145202	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	3141	0.9545371664741993	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2899	0.8809943475354038	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTCTGTT	2280	0.6928827569440223	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT	2012	0.611438643408497	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT	1948	0.5919893028626998	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTA	1523	0.46283352580076587	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTC	1462	0.4442958730930529	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	781	0.23734273384793045	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	620	0.18841548653740958	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATG	484	0.1470856378775907	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCTTCTGT	478	0.14526226220142222	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	432	0.13128304868413057	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGT	412	0.12520512976356896	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTT	390	0.1185194189509512	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	374	0.1136570838145019	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	344	0.10454020543365951	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTAT	340	0.1033246216495472	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	35	1.208391E-7	45.000004	40
CGATCGA	35	1.208391E-7	45.000004	41
CTTCGAC	20	7.02594E-4	45.0	22
CTACTCA	20	7.02594E-4	45.0	29
TTGGACG	45	3.8380676E-10	45.0	1
ACGTTAA	25	3.8847178E-5	45.0	15
TCCGAGG	20	7.02594E-4	45.0	2
GTTTACG	20	7.02594E-4	45.0	1
TAGCGGT	20	7.02594E-4	45.0	2
CGCGCGG	20	7.02594E-4	45.0	2
CGCGATT	20	7.02594E-4	45.0	43
AGGAACG	20	7.02594E-4	45.0	2
TATGATG	20	7.02594E-4	45.0	16
ATCAACC	25	3.8847178E-5	45.0	10
CGTTAAT	25	3.8847178E-5	45.0	16
TCTACTC	20	7.02594E-4	45.0	28
CGCTATT	25	3.8847178E-5	45.0	12
CGAATGC	40	6.7902874E-9	45.0	45
CGGGACG	25	3.8847178E-5	45.0	6
GCGATAT	25	3.8847178E-5	45.0	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content