##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551865_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329060
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.6837871512794	30.0	25.0	31.0	16.0	33.0
2	27.74187990032213	30.0	25.0	33.0	16.0	34.0
3	27.69174314714642	30.0	25.0	33.0	16.0	34.0
4	31.80533945177171	35.0	28.0	35.0	19.0	37.0
5	33.34723758585061	35.0	32.0	35.0	28.0	37.0
6	33.184233878320065	35.0	32.0	35.0	28.0	37.0
7	33.845386859539296	35.0	33.0	36.0	30.0	37.0
8	33.60517838692032	35.0	33.0	37.0	28.0	37.0
9	35.7781103750076	39.0	35.0	39.0	28.0	39.0
10	35.29227800401143	37.0	34.0	39.0	31.0	39.0
11	36.051701817297754	37.0	35.0	39.0	32.0	39.0
12	36.10824165805628	37.0	35.0	39.0	32.0	39.0
13	36.118683522761806	37.0	35.0	39.0	32.0	39.0
14	37.10435482890658	39.0	36.0	40.0	32.0	41.0
15	37.18812374642922	39.0	36.0	40.0	32.0	41.0
16	36.817243055977634	38.0	35.0	40.0	32.0	41.0
17	36.80192670029782	38.0	35.0	40.0	32.0	41.0
18	36.72038837901902	38.0	35.0	40.0	32.0	41.0
19	36.359676046921535	38.0	34.0	40.0	30.0	41.0
20	36.44889381875646	38.0	35.0	40.0	31.0	41.0
21	36.8740017018173	38.0	35.0	40.0	32.0	41.0
22	37.21551996596365	39.0	35.0	40.0	32.0	41.0
23	37.501261168176015	39.0	36.0	40.0	33.0	41.0
24	37.49134808241658	39.0	35.0	41.0	33.0	41.0
25	36.73544946210418	38.0	35.0	40.0	31.0	41.0
26	36.93425211207683	39.0	35.0	40.0	32.0	41.0
27	37.172980611438646	39.0	35.0	40.0	32.0	41.0
28	37.082416580562814	39.0	35.0	40.0	31.0	41.0
29	37.21634048501793	39.0	35.0	41.0	32.0	41.0
30	36.85734820397496	39.0	35.0	40.0	31.0	41.0
31	37.04585485929618	39.0	35.0	40.0	31.0	41.0
32	36.95147389533824	39.0	35.0	40.0	31.0	41.0
33	36.880271075183856	39.0	35.0	41.0	31.0	41.0
34	36.877113596304625	40.0	35.0	41.0	31.0	41.0
35	36.796711845863975	40.0	35.0	41.0	31.0	41.0
36	36.5793958548593	40.0	35.0	41.0	30.0	41.0
37	36.805445815352826	40.0	35.0	41.0	31.0	41.0
38	36.652218440406	39.0	35.0	41.0	31.0	41.0
39	36.53392694341458	39.0	35.0	41.0	31.0	41.0
40	36.47891266030511	39.0	35.0	41.0	30.0	41.0
41	36.1545523612715	39.0	35.0	40.0	29.0	41.0
42	36.44099556311919	39.0	35.0	41.0	30.0	41.0
43	36.39619826171519	39.0	35.0	41.0	30.0	41.0
44	36.58812678538868	39.0	35.0	41.0	31.0	41.0
45	36.573554974776634	39.0	35.0	41.0	31.0	41.0
46	36.44189205615997	39.0	35.0	40.0	31.0	41.0
47	36.243545250106365	39.0	35.0	41.0	30.0	41.0
48	36.39876922141858	39.0	35.0	40.0	31.0	41.0
49	36.418346198261716	39.0	35.0	41.0	31.0	41.0
50	36.215185680423026	39.0	35.0	40.0	30.0	41.0
51	35.39317145809275	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	7.0
11	12.0
12	6.0
13	9.0
14	15.0
15	23.0
16	44.0
17	70.0
18	147.0
19	241.0
20	592.0
21	962.0
22	1395.0
23	1808.0
24	2424.0
25	3117.0
26	3596.0
27	3960.0
28	4231.0
29	4829.0
30	6080.0
31	8407.0
32	11676.0
33	17284.0
34	23532.0
35	27727.0
36	40769.0
37	64401.0
38	78297.0
39	23395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.41597277092324	16.023521546222575	19.709779371543185	16.850726311311007
2	29.379140582264636	32.61198565611135	20.48076338661642	17.528110375007596
3	27.065884641098886	30.240077797362186	26.292469458457425	16.401568103081505
4	25.772503494803377	20.765513888044733	36.03658907190178	17.425393545250106
5	31.17243055977633	24.42077432687048	24.706132620190846	19.70066249316234
6	21.562936850422414	38.307603476569625	26.03689296784781	14.092566705160154
7	65.16197653923297	6.29155777061934	22.494377924998478	6.0520877651492135
8	62.77517777912842	14.94621041755303	14.705524828298792	7.573086975019754
9	57.85571020482587	8.449826779310763	15.198747948702366	18.495715067161004
10	34.7392572783079	27.51443505743633	22.605907737190787	15.140399927064973
11	30.444599769039083	22.735671306144773	28.012824408922388	18.80690451589376
12	25.95392937458214	19.18799003221297	31.567495289612836	23.290585303592053
13	23.914483680787697	21.98504831945542	36.6352640855771	17.465203914179785
14	18.743998055065944	29.22597702546648	29.7684312891266	22.26159363034097
15	15.967604692153406	24.270953625478636	39.26153285115176	20.49990883121619
16	18.389047590105147	25.35950890415122	29.990883121619156	26.260560384124474
17	18.037136084604633	26.802102959946517	33.07755424542636	22.083206710022488
18	18.028626998115847	25.632407463684437	32.6569622561235	23.682003282076217
19	18.930894061873214	26.77870297210235	29.958366255394154	24.33203671063028
20	21.200388986810918	26.563848538260498	35.41846471768066	16.817297757247918
21	20.251018051419194	30.013371421625234	30.90956056646204	18.826049960493528
22	17.614416823679573	23.926031726736767	33.02315687108734	25.436394578496323
23	19.353309426852245	27.930164711602746	32.02668206406127	20.689843797483743
24	21.297331793593873	25.129459673007965	29.14210174436273	24.431106789035432
25	17.482222087157357	32.15614173706923	28.400899532000246	21.96073664377317
26	18.649182519905185	25.480763386616424	32.09961709110801	23.770437002370386
27	22.454871452014828	26.403087582811647	28.10034644137847	23.04169452379505
28	16.355072023339208	25.762171032638427	35.14799732571567	22.73475961830669
29	24.364553576855286	21.730383516683887	30.691059381267856	23.214003525192975
30	18.07694645353431	27.049778155959398	30.4512854798517	24.42198991065459
31	21.24749285844527	23.01160882513827	31.469640794991793	24.271257521424662
32	24.07402905245244	25.896796936728865	29.595818391782654	20.433355619036043
33	18.91873822403209	22.19990275329727	29.246034157904333	29.635324864766304
34	23.91721874430195	22.26037804655686	32.532364918251986	21.2900382908892
35	22.279219595210602	21.506716100407218	28.440102109037866	27.773962195344314
36	21.746793897769404	23.06053607244879	33.70661885370449	21.48605117607731
37	19.015984926761075	22.20841183978606	36.58056281529205	22.195040418160822
38	18.90779796997508	24.07889138758889	29.89910654591868	27.11420409651735
39	20.87522032456087	21.898741870783446	36.74618610587735	20.47985169877834
40	22.909803683218865	21.457484957150672	30.9913085759436	24.641402783686868
41	19.047894001094026	24.049717376770193	33.536741019874796	23.365647602260985
42	21.83461982617152	21.7218744301951	34.394943171458095	22.048562572175285
43	23.2510788306084	21.29945906521607	30.990396888105515	24.459065216070016
44	21.042363094876315	21.459004436880814	31.71579651127454	25.782835956968338
45	19.90640004862335	19.892116939160033	31.408557709840153	28.792925302376464
46	25.059867501367535	24.29739257278308	29.838935148605117	20.803804777244274
47	17.60651552908284	22.045523612715005	38.152920440041335	22.195040418160822
48	20.527259466358718	22.891266030511154	30.777669725885858	25.80380477724427
49	19.904272777001157	19.296176988998965	37.57855710204826	23.22099313195162
50	20.686500942077434	19.312891266030512	33.552847505014284	26.447760286877774
51	20.03646751352337	20.05348568650094	29.97751169999392	29.932535099981767
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	151.0
1	317.0
2	483.0
3	2584.0
4	4685.0
5	3106.0
6	1527.0
7	1464.5
8	1402.0
9	1389.0
10	1376.0
11	1299.5
12	1223.0
13	1178.0
14	1133.0
15	1083.0
16	1033.0
17	1007.0
18	981.0
19	951.0
20	921.0
21	994.5
22	1068.0
23	1313.0
24	1558.0
25	1842.0
26	2550.0
27	2974.0
28	3617.5
29	4261.0
30	4951.5
31	5642.0
32	6332.5
33	7023.0
34	7853.0
35	8683.0
36	9170.0
37	9657.0
38	10360.5
39	11064.0
40	12167.5
41	13271.0
42	14987.5
43	16704.0
44	18050.5
45	19397.0
46	39096.0
47	58795.0
48	45746.5
49	32698.0
50	31833.0
51	30968.0
52	25864.0
53	20760.0
54	18230.5
55	15701.0
56	14106.5
57	12512.0
58	11643.0
59	10774.0
60	9808.5
61	8843.0
62	7867.5
63	6892.0
64	5461.5
65	4031.0
66	3584.5
67	3138.0
68	2570.5
69	2003.0
70	1583.5
71	1164.0
72	1060.0
73	956.0
74	798.0
75	528.5
76	417.0
77	321.5
78	226.0
79	158.5
80	91.0
81	71.5
82	52.0
83	41.0
84	30.0
85	21.0
86	12.0
87	8.0
88	4.0
89	5.0
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	329060.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.68506917220062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.48489927070885	22.65003978160204
2	11.770798364725565	7.45917120796711
3	4.2517945574814835	4.041552139793607
4	2.1413614246703316	2.713967394534461
5	1.340594640295021	2.123841695481458
6	0.9086427723322417	1.7274245456500332
7	0.7560327737653747	1.676846551324462
8	0.6213824379564034	1.575083642323144
9	0.5535589036688316	1.5785596938271025
>10	6.030300974623453	36.701274859796804
>50	0.08976630623772232	1.8576123374343427
>100	0.03889873270301301	2.488404141707726
>500	0.0019948068052827186	0.44275516923196906
>1k	0.006981823818489515	4.824166779675951
>5k	0.0029922102079240776	8.139300059649795
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG	8933	2.7147024858688384	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC	8802	2.67489211693916	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC	8020	2.437245487145202	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	3141	0.9545371664741993	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2899	0.8809943475354038	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTCTGTT	2280	0.6928827569440223	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT	2012	0.611438643408497	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT	1948	0.5919893028626998	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTA	1523	0.46283352580076587	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTC	1462	0.4442958730930529	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	781	0.23734273384793045	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	620	0.18841548653740958	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATG	484	0.1470856378775907	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCTTCTGT	478	0.14526226220142222	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	432	0.13128304868413057	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGT	412	0.12520512976356896	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTT	390	0.1185194189509512	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	374	0.1136570838145019	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	344	0.10454020543365951	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTAT	340	0.1033246216495472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19327782167385887	0.0
2	0.0	0.0	0.0	1.6279705828724245	0.0
3	0.0	0.0	0.0	1.941287303227375	0.0
4	0.0	0.0	0.0	3.0061386981097673	0.0
5	0.0	0.0	0.0	6.810308150489273	0.0
6	0.0	0.0	0.0	7.599222026378168	0.0
7	0.0	0.0	0.0	8.84002917401082	0.0
8	0.0	0.0	0.0	10.209688202759375	0.0
9	0.0	0.0	0.0	10.609311371786301	0.0
10	0.0	0.0	0.0	14.304382179541724	0.0
11	0.0	0.0	0.0	15.642739925849389	0.0
12	0.0	0.0	0.0	19.21959521059989	0.0
13	0.0	0.0	0.0	19.883911748617273	0.0
14	0.0	0.0	0.0	20.187503798699325	0.0
15	0.0	0.0	0.0	21.052391661095243	0.0
16	0.0	0.0	0.0	21.837050993739744	0.0
17	0.0	0.0	0.0	22.677627180453413	0.0
18	0.0	0.0	0.0	23.485990396888106	0.0
19	0.0	0.0	0.0	24.79122348507871	0.0
20	0.0	0.0	0.0	25.53485686500942	0.0
21	0.0	0.0	0.0	26.231994165197836	0.0
22	0.0	0.0	0.0	27.076824895155898	0.0
23	0.0	0.0	0.0	27.756640126420713	0.0
24	0.0	0.0	0.0	28.36352033063879	0.0
25	0.0	0.0	0.0	28.884701878076946	0.0
26	0.0	0.0	0.0	29.390992524159728	0.0
27	0.0	0.0	0.0	29.93678964322616	0.0
28	0.0	0.0	0.0	30.454020543365953	0.0
29	0.0	0.0	0.0	30.97793715431836	0.0
30	0.0	0.0	0.0	31.499726493648573	0.0
31	0.0	0.0	0.0	32.05312101136571	0.0
32	0.0	0.0	0.0	32.553333738527925	0.0
33	0.0	0.0	0.0	33.0584088008266	0.0
34	0.0	0.0	0.0	33.573208533398166	0.0
35	0.0	0.0	0.0	34.1244757794931	0.0
36	0.0	0.0	0.0	34.65203914179785	0.0
37	0.0	0.0	0.0	35.13371421625236	0.0
38	0.0	0.0	0.0	35.650945116392144	0.0
39	0.0	0.0	0.0	36.16483316112563	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	35	1.208391E-7	45.000004	40
CGATCGA	35	1.208391E-7	45.000004	41
CTTCGAC	20	7.02594E-4	45.0	22
CTACTCA	20	7.02594E-4	45.0	29
TTGGACG	45	3.8380676E-10	45.0	1
ACGTTAA	25	3.8847178E-5	45.0	15
TCCGAGG	20	7.02594E-4	45.0	2
GTTTACG	20	7.02594E-4	45.0	1
TAGCGGT	20	7.02594E-4	45.0	2
CGCGCGG	20	7.02594E-4	45.0	2
CGCGATT	20	7.02594E-4	45.0	43
AGGAACG	20	7.02594E-4	45.0	2
TATGATG	20	7.02594E-4	45.0	16
ATCAACC	25	3.8847178E-5	45.0	10
CGTTAAT	25	3.8847178E-5	45.0	16
TCTACTC	20	7.02594E-4	45.0	28
CGCTATT	25	3.8847178E-5	45.0	12
CGAATGC	40	6.7902874E-9	45.0	45
CGGGACG	25	3.8847178E-5	45.0	6
GCGATAT	25	3.8847178E-5	45.0	9
>>END_MODULE