SRR3551863_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551863_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468367
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	16959	3.6208784991256855	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCG	15840	3.3819632894717175	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGC	12388	2.644934421084321	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	6122	1.3070946501354708	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	4195	0.8956651514730969	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3852	0.822431981757895	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCT	3709	0.791900368727942	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTTCTGGT	3564	0.7609417401311365	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTC	2875	0.6138348773504538	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTA	2524	0.5388936453678419	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	2281	0.48701125399526435	No Hit
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	1508	0.32196973740677715	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	869	0.18553826379740673	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATG	831	0.17742496802720945	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTTTCTGG	780	0.16653607107247093	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTT	713	0.15223104958291253	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCC	675	0.14411775381271524	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGT	648	0.13835304366020665	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTAT	558	0.11913734315184461	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	516	0.11017001624794232	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGGC	30	2.1627366E-6	45.000004	4
GTCGTAG	25	3.887253E-5	45.000004	1
AGCGATG	30	2.1627366E-6	45.000004	1
AACGACG	25	3.887253E-5	45.000004	1
ACGTCGG	25	3.887253E-5	45.000004	2
CGGTCGA	30	2.1627366E-6	45.000004	41
CTCCGAT	20	7.0290023E-4	45.0	31
CAACGAA	20	7.0290023E-4	45.0	15
CGACGAA	20	7.0290023E-4	45.0	19
GGCAACG	35	1.2098644E-7	45.0	1
CGTAAGG	45	3.8380676E-10	45.0	2
ACTCCGA	20	7.0290023E-4	45.0	30
CCACCGT	20	7.0290023E-4	45.0	15
CGTTTTT	2110	0.0	44.146923	1
TACGGCT	1385	0.0	44.02527	7
ACGGCTG	1430	0.0	42.797203	8
ACGAAGG	80	0.0	42.1875	2
CGGCTGT	1470	0.0	41.785713	9
CGATGAA	325	0.0	41.538464	19
CGAATAT	65	0.0	41.53846	14

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content