##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551863_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468367
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.71341704261829	30.0	25.0	31.0	16.0	33.0
2	27.811274064996038	30.0	25.0	33.0	16.0	33.0
3	27.61806660161796	30.0	25.0	33.0	16.0	34.0
4	31.7527387710919	35.0	28.0	35.0	19.0	37.0
5	33.30605273215235	35.0	32.0	35.0	28.0	37.0
6	33.29280030403508	35.0	33.0	35.0	28.0	37.0
7	33.7809303388155	35.0	33.0	36.0	30.0	37.0
8	33.626107304741794	35.0	33.0	37.0	28.0	37.0
9	35.569553363067854	37.0	35.0	39.0	28.0	39.0
10	35.19409138560146	37.0	34.0	39.0	31.0	39.0
11	35.99922283166833	37.0	35.0	39.0	32.0	39.0
12	36.042466698123484	37.0	35.0	39.0	32.0	39.0
13	36.06964837403147	37.0	35.0	39.0	32.0	39.0
14	37.12172932764264	39.0	36.0	40.0	32.0	41.0
15	37.15724207725992	39.0	36.0	40.0	32.0	41.0
16	36.66120798433707	38.0	35.0	40.0	32.0	41.0
17	36.711871246266284	38.0	35.0	40.0	32.0	41.0
18	36.64592723227725	38.0	35.0	40.0	32.0	41.0
19	36.19512476327324	37.0	34.0	40.0	30.0	41.0
20	36.33072996176075	38.0	35.0	40.0	30.0	41.0
21	36.83398915807476	38.0	35.0	40.0	32.0	41.0
22	37.18950950856914	39.0	35.0	40.0	32.0	41.0
23	37.46837202450215	39.0	36.0	40.0	33.0	41.0
24	37.44168141649604	39.0	35.0	41.0	32.0	41.0
25	36.72802951531598	38.0	35.0	40.0	31.0	41.0
26	36.94383677756973	39.0	35.0	40.0	32.0	41.0
27	37.198094656540704	39.0	35.0	40.0	32.0	41.0
28	37.101014802494625	39.0	35.0	40.0	31.0	41.0
29	37.305162404695466	39.0	36.0	41.0	32.0	41.0
30	36.947968153179026	39.0	35.0	40.0	31.0	41.0
31	37.0710148238454	39.0	35.0	40.0	31.0	41.0
32	37.011326587910766	39.0	36.0	40.0	31.0	41.0
33	37.00062984796111	39.0	36.0	41.0	31.0	41.0
34	36.963579842303155	40.0	36.0	41.0	31.0	41.0
35	36.88034169785659	40.0	36.0	41.0	31.0	41.0
36	36.625048306136	39.0	35.0	41.0	30.0	41.0
37	36.729654309547854	39.0	35.0	41.0	31.0	41.0
38	36.71890846280801	39.0	35.0	41.0	31.0	41.0
39	36.61298084621675	39.0	35.0	41.0	31.0	41.0
40	36.47132056699127	39.0	35.0	40.0	30.0	41.0
41	36.24584353722615	39.0	35.0	40.0	30.0	41.0
42	36.478432511257196	39.0	35.0	40.0	30.0	41.0
43	36.503852747951925	39.0	35.0	40.0	31.0	41.0
44	36.66407966402415	39.0	35.0	40.0	31.0	41.0
45	36.73949701836381	39.0	36.0	40.0	31.0	41.0
46	36.62366264062156	39.0	35.0	40.0	31.0	41.0
47	36.39852508823209	39.0	35.0	40.0	30.0	41.0
48	36.60558280152103	39.0	35.0	40.0	31.0	41.0
49	36.62479850202939	39.0	35.0	41.0	31.0	41.0
50	36.47049642694724	39.0	35.0	41.0	31.0	41.0
51	35.64045929794371	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	8.0
11	9.0
12	9.0
13	14.0
14	13.0
15	23.0
16	42.0
17	93.0
18	167.0
19	384.0
20	746.0
21	1269.0
22	1775.0
23	2335.0
24	3243.0
25	4226.0
26	5073.0
27	5313.0
28	5765.0
29	6803.0
30	8779.0
31	12025.0
32	16703.0
33	24887.0
34	33407.0
35	40200.0
36	59617.0
37	94299.0
38	110355.0
39	30774.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.7627586913681	15.514543082668078	19.217195062846017	17.50550316311781
2	27.71651290547797	33.687258068992904	20.407287447663904	18.18894157786522
3	25.368354303356128	32.57872565744384	25.8098883994816	16.243031639718424
4	24.92660669944723	19.27270708653684	38.39147506122336	17.409211152792576
5	31.248572166698334	22.781280491580322	25.22807968964509	20.74206765207626
6	21.314055003875165	37.16060268977105	26.985675762809937	14.539666543543845
7	62.41900048466267	6.1101657460922745	24.789961718054432	6.680872051190626
8	58.071127982970616	15.230150715144319	16.95913674533005	9.739584556555009
9	53.427760709016646	8.446581420125671	17.242248066153252	20.88340980470443
10	33.55104010316696	23.85992181344971	25.771029982898025	16.818008100485304
11	28.72789927556809	21.486996308450422	28.874152107215068	20.91095230876642
12	24.71267190045413	17.49205217276196	33.77906641586619	24.016209510917722
13	22.645062525754376	20.58940958692649	39.29290492284896	17.47262296447017
14	18.653321006817304	27.62150194185329	29.526631893365675	24.198545157963732
15	15.69815977641465	23.287080430517097	41.704048321081544	19.31071147198671
16	17.37761200084549	25.772524537382008	28.63011271075887	28.219750751013628
17	17.17029594313861	26.54819831456956	33.38749314106246	22.894012601229377
18	16.526783483891904	25.795156362425192	34.23511904126465	23.442941112418254
19	16.723424152427476	27.090294576688795	29.853725817574677	26.33255545330905
20	18.425294694118076	27.352268626952796	36.928519729186725	17.293916949742403
21	19.470415293989543	30.03862355802181	32.03705641089146	18.45390473709719
22	16.52763751502561	22.430700711194426	33.292695685221204	27.748966088558756
23	17.847115616599805	27.636020471126276	33.67039095410223	20.84647295817169
24	22.37006450070137	23.14595178567235	28.79664878183134	25.687334931794936
25	15.877506314492695	33.13512694105263	27.20857788870693	23.778788855747738
26	16.454190837526983	23.140827598870118	32.525562219370705	27.879419344232193
27	21.57581554635574	25.072005499960504	29.54606110165746	23.806117852026297
28	13.805840291907842	23.655594864710793	36.570894191947765	25.9676706514336
29	19.638659427329426	20.29434183023142	31.848529038126085	28.21846970431307
30	16.81544600708419	25.920058415729546	35.890871901735174	21.37362367545109
31	19.135208074010336	21.512617242461573	33.58968501196711	25.762489671560978
32	21.98745855280154	24.108658381141286	32.09769262138451	21.80619044467266
33	18.90696825352768	20.423727546987724	29.467703745140028	31.201600454344565
34	19.599801010745846	20.198690343256466	35.03620024467992	25.16530840131777
35	22.434757359079523	19.587844574873976	32.10708696385527	25.87031110219123
36	22.917925472973117	21.17121829676301	31.977487739315535	23.933368490948336
37	17.705986971755056	20.150864599768983	42.62149126646412	19.52165716201184
38	19.68840674086774	22.203101414062047	30.25020977139722	27.858282073672996
39	22.165951059745883	19.846402500603162	36.6069770073468	21.380669432304156
40	22.48151556364987	19.94077294087756	32.52278661818617	25.0549248772864
41	22.341454457722257	23.085315575179294	30.523926749749663	24.04930321734879
42	23.294766710720438	19.38351762613506	35.33511114147666	21.98660452166784
43	26.050298163619555	19.577169185702665	28.842552955267987	25.529979695409793
44	20.744629745477372	18.897360403273503	33.079828425145244	27.278181426103888
45	19.19541726893654	17.948318305943843	31.900838445065517	30.955425980054102
46	26.03321754094545	22.415114643004312	30.059760828581005	21.49190698746923
47	16.270360635996987	19.60535221311493	41.012283102780515	23.112004048107572
48	20.800782292518473	21.002120132289424	30.89820589409587	27.298891681096233
49	20.01913029739499	17.233067231465924	39.185724015568994	23.5620784555701
50	22.018630689181773	16.87864431097836	33.89649569675062	27.206229303089245
51	19.755234677080153	17.05179912333704	30.32963466683178	32.863331532751026
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	220.0
1	389.0
2	558.0
3	3422.0
4	6286.0
5	4165.0
6	2044.0
7	2004.0
8	1964.0
9	1854.0
10	1744.0
11	1738.5
12	1733.0
13	1615.0
14	1497.0
15	1485.0
16	1473.0
17	1444.5
18	1416.0
19	1424.5
20	1433.0
21	1482.0
22	1531.0
23	1715.5
24	1900.0
25	2408.5
26	3363.0
27	3809.0
28	4503.5
29	5198.0
30	5783.5
31	6369.0
32	7275.5
33	8182.0
34	9209.0
35	10236.0
36	11231.0
37	12226.0
38	12949.0
39	13672.0
40	15195.0
41	16718.0
42	19037.5
43	21357.0
44	24469.5
45	27582.0
46	62749.5
47	97917.0
48	75150.5
49	52384.0
50	51603.5
51	50823.0
52	40401.5
53	29980.0
54	25754.5
55	21529.0
56	19080.5
57	16632.0
58	14705.0
59	12778.0
60	11635.0
61	10492.0
62	9106.0
63	7720.0
64	6792.0
65	5864.0
66	4650.0
67	3436.0
68	2808.0
69	2180.0
70	1858.5
71	1537.0
72	1256.0
73	975.0
74	872.0
75	643.5
76	518.0
77	424.5
78	331.0
79	275.0
80	219.0
81	172.0
82	125.0
83	76.0
84	27.0
85	29.5
86	32.0
87	23.5
88	15.0
89	8.5
90	2.0
91	8.0
92	14.0
93	8.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	468367.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.943998046957994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3523124968484	24.898707521328745
2	11.5141308032104	7.816712669931852
3	4.1948090155730355	4.271657670959187
4	2.1344607902284074	2.898085315792859
5	1.32969631146699	2.256760449974137
6	0.9414615011220465	1.9174180413223731
7	0.6903626848309091	1.640356873791514
8	0.5842627488372719	1.5865770884354133
9	0.5001534812154443	1.5279487910600653
>10	4.614952360692215	29.225868874175298
>50	0.08717575869837114	1.9412084070648767
>100	0.04358787934918557	2.5628325285805156
>500	0.005053667170920067	1.198647409200559
>1k	0.005053667170920067	5.255179016938694
>5k	6.317083963650083E-4	1.3127226188060506
>10k+	0.001895125189095025	9.689316722637864
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	16959	3.6208784991256855	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCG	15840	3.3819632894717175	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGC	12388	2.644934421084321	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	6122	1.3070946501354708	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	4195	0.8956651514730969	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3852	0.822431981757895	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCT	3709	0.791900368727942	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTTCTGGT	3564	0.7609417401311365	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTC	2875	0.6138348773504538	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTA	2524	0.5388936453678419	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	2281	0.48701125399526435	No Hit
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	1508	0.32196973740677715	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	869	0.18553826379740673	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATG	831	0.17742496802720945	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTTTCTGG	780	0.16653607107247093	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTT	713	0.15223104958291253	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCC	675	0.14411775381271524	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGT	648	0.13835304366020665	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTAT	558	0.11913734315184461	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	516	0.11017001624794232	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.329656017610122	0.0
2	0.0	0.0	0.0	2.490141278100293	0.0
3	0.0	0.0	0.0	3.0083246684757894	0.0
4	0.0	0.0	0.0	4.581236508976935	0.0
5	0.0	0.0	0.0	9.918504079066203	0.0
6	0.0	0.0	0.0	11.023406858297019	0.0
7	0.0	0.0	0.0	12.807264388823295	0.0
8	0.0	0.0	0.0	14.739936844397663	0.0
9	0.0	0.0	0.0	15.296978651356735	0.0
10	0.0	0.0	0.0	19.935221738508478	0.0
11	0.0	0.0	0.0	21.719506284601604	0.0
12	0.0	0.0	0.0	25.896145543985806	0.0
13	0.0	0.0	0.0	26.755087356709588	0.0
14	0.0	0.0	0.0	27.14964974048129	0.0
15	0.0	0.0	0.0	28.17811673324551	0.0
16	0.0	0.0	0.0	29.149577147834925	0.0
17	0.0	0.0	0.0	30.138972216232144	0.0
18	0.0	0.0	0.0	31.187081925071578	0.0
19	0.0	0.0	0.0	32.69658195389513	0.0
20	0.0	0.0	0.0	33.61893557829651	0.0
21	0.0	0.0	0.0	34.54150271048131	0.0
22	0.0	0.0	0.0	35.53623547346419	0.0
23	0.0	0.0	0.0	36.37468053897905	0.0
24	0.0	0.0	0.0	37.045735502287734	0.0
25	0.0	0.0	0.0	37.712306802144475	0.0
26	0.0	0.0	0.0	38.282372583892545	0.0
27	0.0	0.0	0.0	38.923749965304985	0.0
28	0.0	0.0	0.0	39.5266959457007	0.0
29	0.0	0.0	0.0	40.14714956433737	0.0
30	0.0	0.0	0.0	40.769524753024875	0.0
31	0.0	0.0	0.0	41.400226745265996	0.0
32	0.0	0.0	0.0	41.95363891990682	0.0
33	0.0	0.0	0.0	42.53929076984502	0.0
34	0.0	0.0	0.0	43.120672464114676	0.0
35	0.0	0.0	0.0	43.705470282919165	0.0
36	0.0	0.0	0.0	44.24863408395553	0.0
37	0.0	0.0	0.0	44.806316414264884	0.0
38	0.0	0.0	0.0	45.36826890024276	0.0
39	0.0	0.0	0.0	45.92317562936757	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGGC	30	2.1627366E-6	45.000004	4
GTCGTAG	25	3.887253E-5	45.000004	1
AGCGATG	30	2.1627366E-6	45.000004	1
AACGACG	25	3.887253E-5	45.000004	1
ACGTCGG	25	3.887253E-5	45.000004	2
CGGTCGA	30	2.1627366E-6	45.000004	41
CTCCGAT	20	7.0290023E-4	45.0	31
CAACGAA	20	7.0290023E-4	45.0	15
CGACGAA	20	7.0290023E-4	45.0	19
GGCAACG	35	1.2098644E-7	45.0	1
CGTAAGG	45	3.8380676E-10	45.0	2
ACTCCGA	20	7.0290023E-4	45.0	30
CCACCGT	20	7.0290023E-4	45.0	15
CGTTTTT	2110	0.0	44.146923	1
TACGGCT	1385	0.0	44.02527	7
ACGGCTG	1430	0.0	42.797203	8
ACGAAGG	80	0.0	42.1875	2
CGGCTGT	1470	0.0	41.785713	9
CGATGAA	325	0.0	41.538464	19
CGAATAT	65	0.0	41.53846	14
>>END_MODULE