FastQCFastQC Report
Sat 18 Jun 2016
SRR3551862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467413
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCG17830.38146136286324944No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15700.33589138513477373No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTC15660.335035610905131No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC14860.31792012631227634No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC11470.24539326035005446No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5680.12151994060926846No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5120.10953910139427016No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC5050.10804149649239538No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTC1250.045.0000045
TGTAGCG351.2098644E-745.0000041
TAGTGCG351.2098644E-745.0000041
AGCCGAT207.028987E-445.044
ACACGAA207.028987E-445.045
CCGATTC207.028987E-445.045
CGGCATG253.887241E-545.031
CACGCTT207.028987E-445.029
TTCACGC207.028987E-445.027
ATAGAGT253.887241E-545.011
CGATTGC253.887241E-545.010
CGCGAGG1100.045.02
AAACGTC207.028987E-445.011
AGACGGC253.887241E-545.012
GCCGATT253.887241E-545.014
TACGATC207.028987E-445.013
CTTGCGT207.028987E-445.025
ACGGACC207.028987E-445.020
CGAATAT800.045.014
ACGCTTC207.028987E-445.030