##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551862_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	467413
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.23420615173305	28.0	25.0	31.0	16.0	33.0
2	27.71935739913096	31.0	25.0	33.0	16.0	34.0
3	27.545817082537287	31.0	25.0	33.0	16.0	34.0
4	31.6441733541857	35.0	28.0	37.0	19.0	37.0
5	33.18219219405536	35.0	32.0	35.0	28.0	37.0
6	32.86849531356638	35.0	32.0	35.0	28.0	37.0
7	34.150716817889105	35.0	33.0	37.0	31.0	37.0
8	34.05612595285112	35.0	35.0	37.0	31.0	37.0
9	36.42262410330906	39.0	37.0	39.0	32.0	39.0
10	35.476574250181315	37.0	34.0	39.0	31.0	39.0
11	35.97593990753359	37.0	35.0	39.0	31.0	39.0
12	35.93271261175877	37.0	35.0	39.0	31.0	39.0
13	36.05689401022222	37.0	35.0	39.0	32.0	39.0
14	36.916968505368914	39.0	36.0	40.0	32.0	41.0
15	37.20477821541121	39.0	36.0	40.0	32.0	41.0
16	36.713366979523464	38.0	35.0	40.0	32.0	41.0
17	36.974906560151304	38.0	35.0	40.0	32.0	41.0
18	36.706976485463606	38.0	35.0	40.0	32.0	41.0
19	36.309991378074635	38.0	34.0	40.0	30.0	41.0
20	36.86450740565624	38.0	35.0	40.0	32.0	41.0
21	36.896284442238446	39.0	35.0	40.0	32.0	41.0
22	37.29895189051224	39.0	35.0	40.0	33.0	41.0
23	37.67327609630028	39.0	36.0	40.0	33.0	41.0
24	37.74159041361708	39.0	36.0	41.0	33.0	41.0
25	36.599775787151835	38.0	35.0	40.0	31.0	41.0
26	36.97370205792308	39.0	35.0	40.0	32.0	41.0
27	37.30922759957468	39.0	35.0	40.0	33.0	41.0
28	37.38554126650307	39.0	35.0	41.0	33.0	41.0
29	37.5147481991301	39.0	35.0	41.0	33.0	41.0
30	37.084766576881684	39.0	35.0	40.0	32.0	41.0
31	37.22295272061325	39.0	35.0	40.0	33.0	41.0
32	37.19018298592465	39.0	35.0	40.0	33.0	41.0
33	37.29632680306282	39.0	35.0	41.0	33.0	41.0
34	37.340919058733924	40.0	35.0	41.0	33.0	41.0
35	37.29709913930507	40.0	35.0	41.0	33.0	41.0
36	37.16743864633632	40.0	35.0	41.0	33.0	41.0
37	37.22063999075764	40.0	35.0	41.0	33.0	41.0
38	37.05871894876694	39.0	35.0	41.0	33.0	41.0
39	37.00009199572969	39.0	35.0	41.0	32.0	41.0
40	36.86730792682275	39.0	35.0	41.0	32.0	41.0
41	36.71194853373783	39.0	35.0	40.0	31.0	41.0
42	36.83124987965675	39.0	35.0	41.0	32.0	41.0
43	36.773465864235696	39.0	35.0	41.0	32.0	41.0
44	36.79567962380165	39.0	35.0	41.0	32.0	41.0
45	36.8147206004112	39.0	35.0	41.0	32.0	41.0
46	36.635048661462136	39.0	35.0	40.0	32.0	41.0
47	36.62253510278918	39.0	35.0	41.0	32.0	41.0
48	36.60901173052525	39.0	35.0	40.0	32.0	41.0
49	36.58203130850019	39.0	35.0	40.0	32.0	41.0
50	36.4732624039126	39.0	35.0	40.0	32.0	41.0
51	35.55819585676907	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	11.0
10	18.0
11	12.0
12	20.0
13	31.0
14	44.0
15	52.0
16	68.0
17	111.0
18	195.0
19	360.0
20	564.0
21	869.0
22	1207.0
23	1647.0
24	2284.0
25	3179.0
26	3882.0
27	4683.0
28	5186.0
29	6100.0
30	7998.0
31	11373.0
32	16960.0
33	28536.0
34	39127.0
35	38248.0
36	56480.0
37	91073.0
38	112568.0
39	34525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10665942111152	21.69751376191933	23.01711762402843	13.178709192940719
2	33.179864488150734	23.424466157338372	24.643516547464447	18.752152807046446
3	33.21773249781243	22.090314133325347	26.18583565283807	18.50611771602416
4	29.053962983485697	25.72949404488108	25.765008675411256	19.45153429622197
5	26.510601972987484	28.81948084456359	23.957399558848387	20.71251762360054
6	27.020215526739737	34.14111289159693	23.68376574892012	15.154905832743207
7	79.02048081675093	6.115148701469578	9.886117844390293	4.978252637389204
8	78.97533872613727	7.623022894100079	8.631338880176633	4.770299499586019
9	71.99179312513773	9.026706574271575	11.009749407911205	7.971750892679494
10	43.97716794355313	24.381435689636362	18.254947979624013	13.386448387186492
11	34.39570572491565	24.912443599129677	22.73556790247597	17.9562827734787
12	31.17564124232745	23.410345882549265	26.48899367368901	18.925019201434278
13	26.13021032791129	26.991974977161526	27.107718441720706	19.77009625320648
14	19.265189457717266	29.05909762886355	28.966887955619548	22.708824957799635
15	19.669542781223456	26.66742260056952	33.26159092708162	20.401443691125408
16	21.82288468656199	25.2453397744607	31.9255134110519	21.00626212792541
17	22.10785750503302	26.39400273419866	27.941670428507553	23.55646933226076
18	22.859227278659343	27.023638623658307	27.88091045820292	22.236223639479434
19	25.927391835485963	26.965445976042602	25.823629210141778	21.28353297832966
20	27.26881794045095	25.65226042065582	28.12523400076592	18.95368763812731
21	26.08314274528094	27.153716306564	26.318052771317873	20.445088176837185
22	23.72056404079476	25.0731152107451	27.614978616341435	23.591342132118704
23	24.13711214707336	27.142591241578646	27.202281494096226	21.518015117251764
24	23.84978594947081	25.59257016813824	28.365064728623295	22.192579153767653
25	22.224456743821843	28.18406847905386	26.9314289504143	22.66004582671
26	21.640176888533265	28.634205723845934	27.586952010320637	22.13866537730016
27	23.498276684645056	28.174013131855553	28.14448892093288	20.18322126256651
28	20.206755053881682	28.866334483636525	30.40095162094336	20.525958841538426
29	22.117057078001682	25.918406206074714	29.15900927017434	22.805527445749263
30	21.86246424468297	27.679589570679465	28.459413837441407	21.99853234719616
31	23.318991983534904	28.300667717842682	25.304602139863462	23.075738158758956
32	23.612950431417186	28.6395543127812	25.87797087372409	21.869524382077522
33	23.882305370197233	28.490221709708546	26.356562611651796	21.270910308442424
34	21.67248236570228	27.97739900259514	26.27226885003198	24.077849781670597
35	22.92020119252139	25.734200803144113	26.64003782522095	24.705560179113547
36	23.298881289138297	27.70012815219089	26.75043270084486	22.250557857825946
37	22.302332198719334	28.50070494402167	27.69777477305937	21.499188084199627
38	22.08197033458633	26.672771189504786	27.44467954464253	23.800578931266354
39	22.9925141149262	27.010802010213663	27.63808452054179	22.358599354318343
40	22.53531673273957	26.159306651719145	28.735828913616007	22.569547701925277
41	20.840883758046953	26.960953161336974	27.73136391157285	24.466799169043224
42	21.927717029693223	26.40106287159322	27.276947795632555	24.394272303081003
43	23.814913149612867	25.390393506385145	27.36979929954879	23.424894044453193
44	21.824168347906454	27.345409734003972	26.967585411616707	23.862836506472863
45	21.91744773893751	26.939986692710725	25.577808062676905	25.56475750567485
46	21.597602120608542	28.78653353672234	26.95881372576287	22.65705061690625
47	21.998960234310985	26.33024755409028	29.486128969455276	22.184663242143458
48	22.34447907952924	25.106918292815987	29.140610124237025	23.40799250341775
49	21.884500431096267	25.00165806256993	29.301923566524678	23.811917939809117
50	20.637637378506803	26.870241092994846	28.86226955604572	23.629851972452627
51	20.775630973036694	25.83368455734008	26.428661590499196	26.96202287912403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	174.0
1	550.0
2	926.0
3	2044.0
4	3162.0
5	2295.5
6	1429.0
7	1464.5
8	1500.0
9	1501.0
10	1502.0
11	1492.0
12	1482.0
13	1411.0
14	1340.0
15	1252.5
16	1165.0
17	1113.0
18	1061.0
19	1179.5
20	1298.0
21	1333.5
22	1369.0
23	1595.0
24	1821.0
25	2249.5
26	3622.0
27	4566.0
28	5391.5
29	6217.0
30	7134.0
31	8051.0
32	8668.0
33	9285.0
34	10543.0
35	11801.0
36	12911.5
37	14022.0
38	15383.5
39	16745.0
40	19274.0
41	21803.0
42	24089.5
43	26376.0
44	28488.0
45	30600.0
46	36257.0
47	41914.0
48	39458.5
49	37003.0
50	36890.5
51	36778.0
52	33709.5
53	30641.0
54	28723.0
55	26805.0
56	25712.5
57	24620.0
58	24171.0
59	23722.0
60	22777.0
61	21832.0
62	19728.0
63	17624.0
64	15143.0
65	12662.0
66	10703.0
67	8744.0
68	7219.5
69	5695.0
70	4344.0
71	2993.0
72	2667.5
73	2342.0
74	1984.5
75	1314.5
76	1002.0
77	748.5
78	495.0
79	409.5
80	324.0
81	230.5
82	137.0
83	99.5
84	62.0
85	36.0
86	10.0
87	8.5
88	7.0
89	3.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	467413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.758911434471038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.04665727946545	18.580735410353867
2	10.091860359186223	4.997269536842815
3	3.445245725134992	2.559016013358216
4	1.6608823657732104	1.6448655758901458
5	0.9012250328171049	1.1156675385023478
6	0.5635145468168125	0.8371204054004125
7	0.3696083888847126	0.640577095608389
8	0.3237306095975918	0.6412173993323277
9	0.24409616701995077	0.543919984266673
>10	5.722409635671031	39.05143444334031
>50	1.5571948149944808	24.519257665954868
>100	0.06665036173128126	2.910764422856385
>500	0.0025967673401797895	0.3396820505247659
>1k	0.0043279455669663165	1.6184724577684744
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCG	1783	0.38146136286324944	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1570	0.33589138513477373	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTC	1566	0.335035610905131	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC	1486	0.31792012631227634	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	1147	0.24539326035005446	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	568	0.12151994060926846	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	512	0.10953910139427016	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	505	0.10804149649239538	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10076741554043213	0.0
2	0.0	0.0	0.0	0.5973304122906294	0.0
3	0.0	0.0	0.0	0.7774708876304254	0.0
4	0.0	0.0	0.0	1.1165714261263593	0.0
5	0.0	0.0	0.0	2.123389807301038	0.0
6	0.0	0.0	0.0	2.5134089124607146	0.0
7	0.0	0.0	0.0	2.9479282775618136	0.0
8	0.0	0.0	0.0	3.49733533299245	0.0
9	0.0	0.0	0.0	3.70379086589376	0.0
10	0.0	0.0	0.0	4.574327200997833	0.0
11	0.0	0.0	0.0	5.392447364536288	0.0
12	0.0	0.0	0.0	6.279457353560984	0.0
13	0.0	0.0	0.0	6.568709043180228	0.0
14	0.0	0.0	0.0	6.683168846394944	0.0
15	0.0	0.0	0.0	6.959369979012137	0.0
16	0.0	0.0	0.0	7.416353417641358	0.0
17	0.0	0.0	0.0	7.878471501648435	0.0
18	0.0	0.0	0.0	8.393861531450773	0.0
19	0.0	0.0	0.0	8.789657112660539	0.0
20	0.0	0.0	0.0	9.1433058130604	0.0
21	0.0	0.0	0.0	9.511930562479007	0.0
22	0.0	0.0	0.0	9.950300911613498	0.0
23	0.0	0.0	0.0	10.349733533299245	0.0
24	0.0	0.0	0.0	10.678350837482055	0.0
25	0.0	0.0	0.0	10.970383793347638	0.0
26	0.0	0.0	0.0	11.242947885488851	0.0
27	0.0	0.0	0.0	11.53391112356738	0.0
28	0.0	0.0	0.0	11.830222950581177	0.0
29	0.0	0.0	0.0	12.110916897903994	0.0
30	0.0	0.0	0.0	12.4534405333185	0.0
31	0.0	0.0	0.0	12.777565022795686	0.0
32	0.0	0.0	0.0	13.116879504849031	0.0
33	0.0	0.0	0.0	13.437366953850235	0.0
34	0.0	0.0	0.0	13.746515394308673	0.0
35	0.0	0.0	0.0	14.082620723000858	0.0
36	0.0	0.0	0.0	14.397973526624206	0.0
37	0.0	0.0	0.0	14.723809564560678	0.0
38	0.0	0.0	0.0	15.070398127565985	0.0
39	0.0	0.0	0.0	15.401796697995135	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGTC	125	0.0	45.000004	5
TGTAGCG	35	1.2098644E-7	45.000004	1
TAGTGCG	35	1.2098644E-7	45.000004	1
AGCCGAT	20	7.028987E-4	45.0	44
ACACGAA	20	7.028987E-4	45.0	45
CCGATTC	20	7.028987E-4	45.0	45
CGGCATG	25	3.887241E-5	45.0	31
CACGCTT	20	7.028987E-4	45.0	29
TTCACGC	20	7.028987E-4	45.0	27
ATAGAGT	25	3.887241E-5	45.0	11
CGATTGC	25	3.887241E-5	45.0	10
CGCGAGG	110	0.0	45.0	2
AAACGTC	20	7.028987E-4	45.0	11
AGACGGC	25	3.887241E-5	45.0	12
GCCGATT	25	3.887241E-5	45.0	14
TACGATC	20	7.028987E-4	45.0	13
CTTGCGT	20	7.028987E-4	45.0	25
ACGGACC	20	7.028987E-4	45.0	20
CGAATAT	80	0.0	45.0	14
ACGCTTC	20	7.028987E-4	45.0	30
>>END_MODULE