##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551858_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	377049
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.545576304406058	28.0	25.0	31.0	16.0	33.0
2	27.712459123350015	31.0	25.0	33.0	16.0	34.0
3	27.584085888041077	31.0	25.0	33.0	16.0	34.0
4	31.545990043734367	35.0	28.0	37.0	19.0	37.0
5	33.11869544807173	35.0	32.0	35.0	28.0	37.0
6	32.891486252449944	35.0	32.0	35.0	28.0	37.0
7	34.09511230635806	35.0	33.0	37.0	31.0	37.0
8	34.348315470933485	35.0	35.0	37.0	31.0	37.0
9	36.63649021745184	39.0	37.0	39.0	32.0	39.0
10	35.47493827062265	37.0	34.0	39.0	31.0	39.0
11	35.89396868841981	37.0	35.0	39.0	31.0	39.0
12	35.86648419701418	37.0	35.0	39.0	31.0	39.0
13	35.9632912433132	37.0	35.0	39.0	31.0	39.0
14	36.760622624645606	39.0	36.0	40.0	31.0	41.0
15	37.14865972327204	39.0	36.0	40.0	32.0	41.0
16	36.68301732666046	38.0	35.0	40.0	31.0	41.0
17	36.92977835772008	38.0	35.0	40.0	32.0	41.0
18	36.79446172778604	38.0	35.0	40.0	32.0	41.0
19	36.3031011884397	38.0	34.0	40.0	30.0	41.0
20	36.81421512853767	38.0	35.0	40.0	32.0	41.0
21	36.80075533949169	38.0	35.0	40.0	32.0	41.0
22	37.20184909653653	39.0	35.0	40.0	32.0	41.0
23	37.577012536832086	39.0	35.0	40.0	33.0	41.0
24	37.63716121777276	39.0	35.0	41.0	33.0	41.0
25	36.51209259273993	38.0	35.0	40.0	31.0	41.0
26	36.877909767695975	38.0	35.0	40.0	32.0	41.0
27	37.281016525703556	39.0	35.0	40.0	33.0	41.0
28	37.36617256643036	39.0	35.0	40.0	33.0	41.0
29	37.507865025500664	39.0	35.0	41.0	33.0	41.0
30	37.06877090245565	39.0	35.0	40.0	32.0	41.0
31	37.20383557574745	39.0	35.0	40.0	33.0	41.0
32	37.14563889574034	39.0	35.0	40.0	33.0	41.0
33	37.240647767266324	39.0	35.0	41.0	33.0	41.0
34	37.24977920641614	40.0	35.0	41.0	33.0	41.0
35	37.18145652156616	40.0	35.0	41.0	33.0	41.0
36	37.073637643913656	40.0	35.0	41.0	33.0	41.0
37	37.1235674938801	40.0	35.0	41.0	33.0	41.0
38	36.955178239433074	39.0	35.0	41.0	32.0	41.0
39	36.93919357961432	39.0	35.0	41.0	32.0	41.0
40	36.80291155791422	39.0	35.0	41.0	32.0	41.0
41	36.68312341366772	39.0	35.0	41.0	31.0	41.0
42	36.81005121350275	39.0	35.0	41.0	32.0	41.0
43	36.79052059546637	39.0	35.0	41.0	32.0	41.0
44	36.82075008818482	39.0	35.0	41.0	32.0	41.0
45	36.79637129391671	39.0	35.0	41.0	32.0	41.0
46	36.645931961097894	39.0	35.0	40.0	32.0	41.0
47	36.599187373524394	39.0	35.0	41.0	32.0	41.0
48	36.6246429509162	39.0	35.0	41.0	32.0	41.0
49	36.623595341719515	39.0	35.0	40.0	32.0	41.0
50	36.54992852387886	39.0	35.0	41.0	32.0	41.0
51	35.63246421552636	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	14.0
11	11.0
12	18.0
13	10.0
14	23.0
15	39.0
16	45.0
17	90.0
18	138.0
19	260.0
20	410.0
21	659.0
22	968.0
23	1434.0
24	2023.0
25	2717.0
26	3474.0
27	3867.0
28	4203.0
29	5063.0
30	6361.0
31	9014.0
32	13899.0
33	23459.0
34	32894.0
35	30176.0
36	43550.0
37	72215.0
38	91493.0
39	28513.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.50122663102143	22.575049927197792	25.729281870526112	14.194441571254664
2	32.82782874374418	24.528111730836045	24.564446530822252	18.079612994597518
3	32.67957215109972	22.140358414954026	27.4073131078454	17.772756326100854
4	29.190900917387395	24.72251617163817	27.406517455290956	18.68006545568348
5	27.754748056618634	28.2106569703142	24.600516113290315	19.434078859776847
6	25.785507984373385	33.60438563688008	25.15641203132749	15.453694347419036
7	78.82344204599401	6.174263822474002	10.861983455731218	4.140310675800758
8	80.60702985553601	6.2023768793976375	8.8465955353283	4.343997729738044
9	75.04700980509165	6.388824794655338	10.659092054348374	7.905073345904643
10	45.5951348498471	20.774488196494357	18.830709005991263	14.799667947667281
11	33.68925524268729	24.919042352585475	24.64799004903872	16.743712355688515
12	30.354675387018666	22.69970216072712	28.371378786311592	18.57424366594262
13	23.94092014565746	27.42667398667017	29.626122864667458	19.006283003004913
14	20.09579656755488	29.887362120042756	29.279218350930513	20.737622961471853
15	18.810287257093904	26.98057812114606	35.68263010908396	18.52650451267607
16	21.428249378727966	25.75076448949606	32.15364581261321	20.66734031916276
17	20.778731676784716	26.552782264374127	29.51367063697291	23.154815421868243
18	22.20268453171869	26.46419961331286	31.31821063044856	20.01490522451989
19	24.145668069667337	27.053247721118474	28.18519608857204	20.615888120642147
20	24.85883797596599	26.828608483247535	30.066649162310473	18.24590437847601
21	23.49906776042371	27.986548167479558	29.355335778638853	19.15904829345788
22	22.475858575410623	25.015581529191167	29.98177955650327	22.526780338894945
23	21.779132155237118	26.637121435145033	29.71046203543837	21.873284374179484
24	21.825014785876636	25.40916432612207	32.04702837031792	20.71879251768338
25	19.888926903399824	28.971300812361257	29.694814201867665	21.444958082371258
26	19.806444255255947	29.894522993032734	29.186922654615184	21.112110097096135
27	20.640288132311717	29.47017496399672	29.377348832645094	20.51218807104647
28	18.367639219305715	28.047282979135336	32.57905471172182	21.006023089837132
29	20.377192354309386	27.039456410174807	30.332397115494274	22.250954120021536
30	20.436335860856282	29.452405390280838	28.551991916170046	21.559266832692835
31	23.450532954602718	27.817339390901445	26.467647441048776	22.264480213447058
32	22.936276186914696	28.895183384652924	26.58248662640665	21.58605380202573
33	22.47081944256582	28.526531034427887	26.306925625051385	22.69572389795491
34	20.969423072332773	28.25972221117149	27.25481303491058	23.51604168158515
35	20.235300982100473	27.86932202445836	27.893191601091633	24.002185392349535
36	21.946749626706342	28.32947441844429	28.420709244687032	21.303066710162337
37	20.28197926529443	28.65701805335646	28.73499200369183	22.326010677657283
38	20.743457746871098	29.726375086527216	26.30215170972473	23.228015456876957
39	21.116353577386494	27.975409031717362	28.066643857960106	22.841593532936038
40	20.565496792194118	29.04715302255145	28.288365703131426	22.09898448212301
41	18.896748168009996	27.598269720911606	28.714835472312615	24.790146638765783
42	20.63020986662211	27.037069452511474	29.326427069160772	23.00629361170564
43	21.24233189850656	25.99768199889139	28.992518213813057	23.76746788878899
44	20.666014231572024	27.28292609183422	27.666695840593665	24.384363836000094
45	20.760696885550683	28.144352590777327	27.363021782314767	23.731928741357226
46	22.399741147702287	28.483300578969846	27.52878273115696	21.588175542170912
47	19.163822208784534	27.50623924211442	30.705027728491523	22.62491082060952
48	20.656996835955006	26.49469962789982	29.61312720627823	23.235176329866942
49	20.156796596728807	25.465390439969344	31.525345512121767	22.852467451180086
50	20.233709676991584	26.673986670167537	29.850762102538397	23.24154155030248
51	20.02047479240099	26.71801277818002	27.269134780890546	25.992377648528443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	147.0
1	417.0
2	687.0
3	1890.0
4	3093.0
5	2213.0
6	1333.0
7	1336.0
8	1339.0
9	1412.5
10	1486.0
11	1421.0
12	1356.0
13	1267.0
14	1178.0
15	1069.5
16	961.0
17	948.0
18	935.0
19	1039.0
20	1143.0
21	1204.5
22	1266.0
23	1395.5
24	1525.0
25	1862.0
26	2920.5
27	3642.0
28	4456.5
29	5271.0
30	6345.5
31	7420.0
32	7959.0
33	8498.0
34	10093.0
35	11688.0
36	12206.0
37	12724.0
38	13799.5
39	14875.0
40	16199.0
41	17523.0
42	20273.5
43	23024.0
44	25680.0
45	28336.0
46	33910.0
47	39484.0
48	37482.0
49	35480.0
50	33918.5
51	32357.0
52	29491.5
53	26626.0
54	24732.0
55	22838.0
56	19919.0
57	17000.0
58	15146.0
59	13292.0
60	12549.0
61	11806.0
62	10380.0
63	8954.0
64	7582.0
65	6210.0
66	5124.5
67	4039.0
68	3345.5
69	2652.0
70	2249.5
71	1847.0
72	1546.5
73	1246.0
74	949.5
75	485.5
76	318.0
77	335.0
78	352.0
79	223.0
80	94.0
81	76.0
82	58.0
83	59.0
84	60.0
85	38.5
86	17.0
87	12.0
88	7.0
89	5.5
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	377049.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.756448469291012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.80887342858198	19.748633184682767
2	10.616464543811118	5.681177729850746
3	3.6336197367057745	2.916682777265005
4	1.7487167657219231	1.8715780011769556
5	0.9968363135784617	1.3335899728290064
6	0.6168986476382037	0.9903610125784147
7	0.47592180757348446	0.891378412382624
8	0.40193396348533367	0.8603460305642581
9	0.3099490763179212	0.7463822839744325
>10	6.426944072810245	45.28132778936833
>50	0.9178492001928754	15.168146637654978
>100	0.038993593472246346	2.1134122019343256
>500	0.0029995071901727957	0.5307607352705952
>1k	0.003999342920230394	1.8662232304675765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTC	1963	0.5206219881235595	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCG	1740	0.46147848157666504	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGC	1644	0.4360175998345043	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1629	0.43203933706229164	No Hit
GCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	839	0.22251749772575977	No Hit
CCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	587	0.1556826831525876	No Hit
GAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT	558	0.14799137512630983	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGCACATT	425	0.1127174452126912	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC	406	0.10767831236788852	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT	396	0.10502613718641343	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6521751814750867E-4	0.0	0.0	0.09335656638792306	0.0
2	2.6521751814750867E-4	0.0	0.0	0.6572090099695265	0.0
3	2.6521751814750867E-4	0.0	0.0	0.8834395529493514	0.0
4	2.6521751814750867E-4	0.0	0.0	1.3984919731918133	0.0
5	2.6521751814750867E-4	0.0	0.0	2.8205883054987546	0.0
6	2.6521751814750867E-4	0.0	0.0	3.4000885826510614	0.0
7	2.6521751814750867E-4	0.0	0.0	3.9785279897307775	0.0
8	2.6521751814750867E-4	0.0	0.0	4.74155878944116	0.0
9	2.6521751814750867E-4	0.0	0.0	4.998289347007948	0.0
10	2.6521751814750867E-4	0.0	0.0	6.0917811743301264	0.0
11	2.6521751814750867E-4	0.0	0.0	7.226116499447022	0.0
12	2.6521751814750867E-4	0.0	0.0	8.42702142161894	0.0
13	2.6521751814750867E-4	0.0	0.0	8.802569427315813	0.0
14	2.6521751814750867E-4	0.0	0.0	8.957721675432106	0.0
15	2.6521751814750867E-4	0.0	0.0	9.294547923479442	0.0
16	2.6521751814750867E-4	0.0	0.0	9.852035146625505	0.0
17	2.6521751814750867E-4	0.0	0.0	10.490413712806559	0.0
18	2.6521751814750867E-4	0.0	0.0	11.143644460003872	0.0
19	2.6521751814750867E-4	0.0	0.0	11.651536007256352	0.0
20	2.6521751814750867E-4	0.0	0.0	12.06474490053017	0.0
21	2.6521751814750867E-4	0.0	0.0	12.562823399611192	0.0
22	2.6521751814750867E-4	0.0	0.0	13.055597548329262	0.0
23	2.6521751814750867E-4	0.0	0.0	13.582054321852066	0.0
24	2.6521751814750867E-4	0.0	0.0	14.024702359640258	0.0
25	2.6521751814750867E-4	0.0	0.0	14.412715588690064	0.0
26	2.6521751814750867E-4	0.0	0.0	14.756702709727383	0.0
27	2.6521751814750867E-4	0.0	0.0	15.120846362143912	0.0
28	2.6521751814750867E-4	0.0	0.0	15.503555240830767	0.0
29	2.6521751814750867E-4	0.0	0.0	15.861333672811757	0.0
30	2.6521751814750867E-4	0.0	0.0	16.28249909163	0.0
31	2.6521751814750867E-4	0.0	0.0	16.67873406374238	0.0
32	2.6521751814750867E-4	0.0	0.0	17.078416863590675	0.0
33	2.6521751814750867E-4	0.0	0.0	17.4693474853401	0.0
34	2.6521751814750867E-4	0.0	0.0	17.846752013664005	0.0
35	2.6521751814750867E-4	0.0	0.0	18.27216091277261	0.0
36	2.6521751814750867E-4	0.0	0.0	18.675556757874972	0.0
37	2.6521751814750867E-4	0.0	0.0	19.048717805908517	0.0
38	2.6521751814750867E-4	0.0	0.0	19.43567016488573	0.0
39	2.6521751814750867E-4	0.0	0.0	19.82209208882665	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCGAA	30	2.161607E-6	45.000004	2
GCGCCCA	30	2.161607E-6	45.000004	1
TATAGCG	45	3.8380676E-10	45.000004	1
GTACCGG	20	7.02725E-4	45.0	1
CGAACGG	25	3.885802E-5	45.0	2
GCGACAT	25	3.885802E-5	45.0	12
CTCGTAG	25	3.885802E-5	45.0	1
GGTAACG	25	3.885802E-5	45.0	9
TAAAGCG	20	7.02725E-4	45.0	27
TAGCGTG	35	1.2090277E-7	45.0	1
CGTGACT	25	3.885802E-5	45.0	15
TAGCGCG	20	7.02725E-4	45.0	1
CCCAGTA	20	7.02725E-4	45.0	29
CACGCAT	20	7.02725E-4	45.0	22
GCGCCTT	25	3.885802E-5	45.0	10
TAACGGA	20	7.02725E-4	45.0	34
CTGGTAA	20	7.02725E-4	45.0	10
AATGCGT	20	7.02725E-4	45.0	14
CGATGGA	20	7.02725E-4	45.0	10
CCGCGCA	20	7.02725E-4	45.0	21
>>END_MODULE