##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551852_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	361872
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.247236591944112	28.0	25.0	31.0	16.0	33.0
2	27.877462196577795	31.0	25.0	33.0	16.0	34.0
3	27.679834306052967	31.0	25.0	33.0	16.0	34.0
4	31.612619931909627	35.0	28.0	37.0	19.0	37.0
5	33.18684507229076	35.0	32.0	35.0	28.0	37.0
6	32.86839545474643	35.0	32.0	35.0	28.0	37.0
7	34.06440951496662	35.0	33.0	37.0	31.0	37.0
8	34.26077729141796	35.0	35.0	37.0	31.0	37.0
9	36.49367455896007	39.0	37.0	39.0	32.0	39.0
10	35.4681268514834	37.0	34.0	39.0	31.0	39.0
11	35.93533625149224	37.0	35.0	39.0	31.0	39.0
12	35.80620219304063	37.0	35.0	39.0	31.0	39.0
13	35.91890779059999	37.0	35.0	39.0	31.0	39.0
14	36.67023422646682	38.0	35.0	40.0	31.0	41.0
15	37.07355915903966	39.0	35.0	40.0	32.0	41.0
16	36.62291362691781	38.0	35.0	40.0	31.0	41.0
17	36.91552261573153	38.0	35.0	40.0	32.0	41.0
18	36.68376939912455	38.0	35.0	40.0	32.0	41.0
19	36.197895941106246	38.0	34.0	40.0	30.0	41.0
20	36.805527921475	38.0	35.0	40.0	32.0	41.0
21	36.75341004554097	38.0	35.0	40.0	32.0	41.0
22	37.162601693416455	39.0	35.0	40.0	32.0	41.0
23	37.55425951717734	39.0	35.0	40.0	33.0	41.0
24	37.639516182517575	39.0	35.0	41.0	33.0	41.0
25	36.5926819427864	38.0	35.0	40.0	31.0	41.0
26	36.87183589777601	38.0	35.0	40.0	32.0	41.0
27	37.229921077065924	39.0	35.0	40.0	33.0	41.0
28	37.33048978644383	39.0	35.0	40.0	33.0	41.0
29	37.41579895653712	39.0	35.0	41.0	33.0	41.0
30	37.002879471194234	39.0	35.0	40.0	32.0	41.0
31	37.07363929787328	39.0	35.0	40.0	33.0	41.0
32	37.02039118804439	39.0	35.0	40.0	33.0	41.0
33	37.164411725693064	39.0	35.0	41.0	33.0	41.0
34	37.15150384666401	39.0	35.0	41.0	33.0	41.0
35	37.08034885263297	39.0	35.0	41.0	33.0	41.0
36	36.97358181898572	39.0	35.0	41.0	33.0	41.0
37	37.012656408895964	39.0	35.0	41.0	33.0	41.0
38	36.84570511119954	39.0	35.0	41.0	32.0	41.0
39	36.78712086041473	39.0	35.0	41.0	32.0	41.0
40	36.657337401069995	39.0	35.0	40.0	32.0	41.0
41	36.5308645045762	39.0	35.0	40.0	31.0	41.0
42	36.660675598001504	39.0	35.0	40.0	32.0	41.0
43	36.626069438917625	39.0	35.0	40.0	32.0	41.0
44	36.62761418402087	39.0	35.0	40.0	32.0	41.0
45	36.62243003050803	39.0	35.0	40.0	32.0	41.0
46	36.486882101958706	39.0	35.0	40.0	32.0	41.0
47	36.46725361453774	39.0	35.0	40.0	32.0	41.0
48	36.43985442366362	39.0	35.0	40.0	32.0	41.0
49	36.41757859132511	38.0	35.0	40.0	32.0	41.0
50	36.30984160145024	38.0	35.0	40.0	31.0	41.0
51	35.376763054339655	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	16.0
10	13.0
11	27.0
12	21.0
13	18.0
14	32.0
15	39.0
16	51.0
17	76.0
18	137.0
19	267.0
20	399.0
21	595.0
22	852.0
23	1313.0
24	1781.0
25	2603.0
26	3273.0
27	3801.0
28	4143.0
29	4970.0
30	6617.0
31	9008.0
32	14030.0
33	23972.0
34	33117.0
35	29866.0
36	42870.0
37	68907.0
38	83529.0
39	25525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.00214440465137	20.484591236680373	23.291384799044966	14.221879559623293
2	34.88747402396427	21.76598355219525	25.220519962859793	18.12602246098068
3	33.101207056638806	20.481551487818898	28.116018923818366	18.301222531723923
4	29.86415085997259	25.04725427775567	25.612371225184592	19.47622363708715
5	26.075794756156874	28.838926471238445	24.606767033647255	20.478511738957422
6	26.565747004465667	33.361243754697796	24.36413980634036	15.708869434496176
7	78.2304240173321	6.117356413317416	10.492936729009154	5.159282840341336
8	78.98870318786754	5.8692023698987486	9.87752575496308	5.2645686872706365
9	72.38692134235309	7.982104169430075	12.180550028739443	7.450424459477384
10	44.30240527037184	22.660498739885927	19.644515187690676	13.392580802051555
11	35.31524959101561	24.03943935977362	23.222299597647787	17.423011451562985
12	31.88834726090993	21.945881416633505	27.43235177079188	18.733419551664674
13	24.801587301587304	26.923332006897464	28.871534686297917	19.403546005217315
14	19.57487730468232	29.733994340540303	29.79368395454746	20.897444400229915
15	20.118439669275322	26.237177786620684	34.82971879559623	18.81466374850776
16	22.208405181942787	23.674393155590927	34.58930229473405	19.52789936773224
17	22.86609629924393	24.10880090197639	28.94587036300128	24.079232435778398
18	22.473692355307954	26.900119379228016	30.180561082371664	20.445627183092363
19	25.722354865808907	26.648096564531105	27.16623557500995	20.463312994650042
20	27.87670778617854	26.230545607286555	27.708968917186187	18.18377768934872
21	25.74888358314542	26.034067294512976	28.02482645797409	20.192222664367513
22	22.42063492063492	25.32746385462263	28.781171242870407	23.470729981872044
23	22.88129504355131	25.959178936198434	29.140137949330146	22.019388070920105
24	23.670248043507097	24.17595171773445	30.385882300924084	21.767917937834373
25	22.333863907680062	25.590816642348674	28.80659459698457	23.26872485298669
26	20.415229694477606	28.206382367245876	29.05861741168148	22.31977052659504
27	21.717900252022815	28.233463766193573	28.418059424326835	21.63057655745678
28	20.294745103240924	27.84354688950789	30.048470177300263	21.81323782995092
29	21.15886280231684	26.7315514878189	29.947053101649203	22.16253260821506
30	23.006477428483	26.23551974178715	28.056329309811208	22.701673519918643
31	24.598200468674005	27.19138258831852	26.2098200468674	22.00059689614007
32	25.766016713091922	26.887407702170936	26.448025821284876	20.89854976345227
33	25.517586328867665	27.401401600565944	25.276617146394305	21.804394924172083
34	21.77233939072379	26.41237785736393	28.70075606844409	23.11452668346819
35	22.152031657602688	26.521256134765885	27.52547862227528	23.801233585356147
36	23.689591899898307	26.520427112349115	27.130311270283414	22.65966971746916
37	22.879084317106603	28.36942344254322	28.430218419772736	20.321273820577442
38	23.680472653313878	27.61722376973073	26.962572401291062	21.739731175664325
39	23.91038820356369	25.77181987000928	28.934540389972145	21.38325153645488
40	23.631560330724678	24.453121545739933	30.100974930362117	21.814343193173276
41	20.40970287836583	26.123878056329307	28.59160145023655	24.874817615068313
42	20.910432418092586	25.961666003448734	28.489355352168722	24.63854622628996
43	22.26781845514436	25.03205553344829	29.46483839589689	23.235287615510455
44	22.310651280010614	25.465910598222575	28.53826767475793	23.68517044700889
45	22.588097448821685	26.39911349869567	27.24361100057479	23.769178051907858
46	22.008610779502146	26.481186717955516	28.412256267409468	23.097946235132866
47	21.027048238051023	26.837390016359375	30.047088473272314	22.088473272317284
48	22.802537913958528	23.93829862492815	30.32867975416722	22.930483706946102
49	21.859663085289828	23.916467701286642	30.594795949949155	23.62907326347438
50	20.19719679886811	25.437723836052527	29.89261396294822	24.472465402131142
51	20.834715037361278	26.061148693460673	27.367411681478533	25.736724587699516
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	183.0
1	452.5
2	722.0
3	1767.0
4	2812.0
5	2008.0
6	1204.0
7	1252.0
8	1300.0
9	1334.0
10	1368.0
11	1386.0
12	1404.0
13	1344.5
14	1285.0
15	1108.5
16	932.0
17	989.0
18	1046.0
19	1125.5
20	1205.0
21	1114.5
22	1024.0
23	1361.0
24	1698.0
25	1892.5
26	2307.0
27	2527.0
28	3221.0
29	3915.0
30	4444.0
31	4973.0
32	5829.0
33	6685.0
34	6912.0
35	7139.0
36	8097.5
37	9056.0
38	11027.0
39	12998.0
40	14371.5
41	15745.0
42	18374.0
43	21003.0
44	23383.5
45	25764.0
46	28533.0
47	31302.0
48	32529.5
49	33757.0
50	33049.0
51	32341.0
52	28883.0
53	25425.0
54	23358.0
55	21291.0
56	20566.0
57	19841.0
58	19336.0
59	18831.0
60	17385.5
61	15940.0
62	14627.5
63	13315.0
64	11038.0
65	8761.0
66	6976.5
67	5192.0
68	4171.0
69	3150.0
70	2563.5
71	1977.0
72	1510.5
73	1044.0
74	911.5
75	624.0
76	469.0
77	353.5
78	238.0
79	143.0
80	48.0
81	32.5
82	17.0
83	21.5
84	26.0
85	34.5
86	43.0
87	24.0
88	5.0
89	4.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	361872.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.44616881107132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41602899718083	20.424348941062036
2	10.648409182440597	5.84516071981253
3	3.6890857833266213	3.037538135031171
4	1.7579540877970197	1.9299641862315957
5	1.0239629480467176	1.4051929964186232
6	0.6282722513089005	1.0346199761241544
7	0.4510672573499798	0.8666047663262147
8	0.3493757551349174	0.7671220763142769
9	0.2778896496173983	0.6864305610823717
>10	5.596053161498188	40.83018304814962
>50	1.0984695932339912	18.772383605252685
>100	0.05638340716874748	2.679953132599372
>500	0.006041079339508659	1.2819449971260557
>1k	0.0010068465565847764	0.438552858469293
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1587	0.438552858469293	No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	978	0.2702613078657647	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	830	0.22936286863863464	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	790	0.21830923641508598	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	763	0.21084803466419064	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	663	0.18321395410531902	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT	615	0.16994959543706062	No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG	406	0.11219436706901888	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	389	0.10749657337401071	No Hit
TCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	380	0.10500950612371227	No Hit
CGTGAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC	367	0.10141707565105894	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17575275235442367	0.0
2	0.0	0.0	0.0	0.8666047663262147	0.0
3	0.0	0.0	0.0	1.1379714374143344	0.0
4	0.0	0.0	0.0	1.5654706636600788	0.0
5	0.0	0.0	0.0	2.726654728743865	0.0
6	0.0	0.0	0.0	3.2099747977185302	0.0
7	0.0	0.0	0.0	3.7955409647610203	0.0
8	0.0	0.0	0.0	4.672923022505195	0.0
9	0.0	0.0	0.0	4.955066985011275	0.0
10	0.0	0.0	0.0	5.857872396869611	0.0
11	0.0	0.0	0.0	6.954669054251227	0.0
12	0.0	0.0	0.0	7.929875757173807	0.0
13	0.0	0.0	0.0	8.333886014944511	0.0
14	0.0	0.0	0.0	8.46321351196003	0.0
15	0.0	0.0	0.0	8.776583985497634	0.0
16	0.0	0.0	0.0	9.320975372507405	0.0
17	0.0	0.0	0.0	9.994970597338286	0.0
18	0.0	0.0	0.0	10.698534288367158	0.0
19	0.0	0.0	0.0	11.165550249812089	0.0
20	0.0	0.0	0.0	11.598299951364018	0.0
21	0.0	0.0	0.0	12.104832648008136	0.0
22	0.0	0.0	0.0	12.686530043772384	0.0
23	0.0	0.0	0.0	13.225118273864792	0.0
24	0.0	0.0	0.0	13.644050935137287	0.0
25	0.0	0.0	0.0	14.025953928460892	0.0
26	0.0	0.0	0.0	14.37082725383561	0.0
27	0.0	0.0	0.0	14.738360525268604	0.0
28	0.0	0.0	0.0	15.112249635230137	0.0
29	0.0	0.0	0.0	15.494981650970509	0.0
30	0.0	0.0	0.0	15.887109254100897	0.0
31	0.0	0.0	0.0	16.29802803201132	0.0
32	0.0	0.0	0.0	16.703419993809966	0.0
33	0.0	0.0	0.0	17.086428350355927	0.0
34	0.0	0.0	0.0	17.467502321262767	0.0
35	0.0	0.0	0.0	17.869301852588762	0.0
36	0.0	0.0	0.0	18.27054870230358	0.0
37	0.0	0.0	0.0	18.693073794048725	0.0
38	0.0	0.0	0.0	19.104545253570322	0.0
39	0.0	0.0	0.0	19.50966087456338	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGA	30	2.1613632E-6	45.000004	14
GTTGTAG	55	1.8189894E-12	45.000004	1
TTCTAGG	20	7.0268736E-4	45.0	2
TTGTGCG	35	1.2088458E-7	45.0	1
CGTGCGG	25	3.88549E-5	45.0	2
ATAATCG	20	7.0268736E-4	45.0	41
GACCGTT	25	3.88549E-5	45.0	7
ATTACAC	20	7.0268736E-4	45.0	28
TAACGGA	25	3.88549E-5	45.0	35
TAACGAA	20	7.0268736E-4	45.0	35
TCGATCA	20	7.0268736E-4	45.0	17
CCGCTAC	25	3.88549E-5	45.0	20
TAGTTGG	70	0.0	45.0	2
TGTTGCG	45	3.8380676E-10	45.0	1
GCACAAT	20	7.0268736E-4	45.0	10
ACGGCTA	25	3.88549E-5	45.0	5
GTTCGCA	20	7.0268736E-4	45.0	12
ACTACGG	25	3.88549E-5	45.0	2
ACTACGA	20	7.0268736E-4	45.0	31
CTGTTAC	20	7.0268736E-4	45.0	41
>>END_MODULE