##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551850_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	833155
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.23748162106691	28.0	25.0	31.0	16.0	33.0
2	27.69933685808763	31.0	25.0	33.0	16.0	34.0
3	27.479493011504463	31.0	25.0	33.0	16.0	34.0
4	31.637827295041138	35.0	28.0	37.0	19.0	37.0
5	33.17716751384796	35.0	32.0	35.0	28.0	37.0
6	32.912893759264485	35.0	32.0	35.0	28.0	37.0
7	34.20994532830026	35.0	33.0	37.0	32.0	37.0
8	34.24486920200923	35.0	35.0	37.0	31.0	37.0
9	36.63114786564325	39.0	37.0	39.0	32.0	39.0
10	35.55511879542222	37.0	34.0	39.0	31.0	39.0
11	36.04123002322497	37.0	35.0	39.0	31.0	39.0
12	36.02511417443333	37.0	35.0	39.0	31.0	39.0
13	36.23606291746434	37.0	35.0	39.0	32.0	39.0
14	37.12761971061807	39.0	36.0	40.0	32.0	41.0
15	37.36531017637775	39.0	36.0	40.0	32.0	41.0
16	36.84527728933992	38.0	35.0	40.0	32.0	41.0
17	37.09662667810912	38.0	36.0	40.0	32.0	41.0
18	36.87886647742617	38.0	35.0	40.0	32.0	41.0
19	36.47354453853124	38.0	34.0	40.0	30.0	41.0
20	37.06360281100155	38.0	35.0	40.0	32.0	41.0
21	37.062474569557885	39.0	35.0	40.0	32.0	41.0
22	37.509969933565785	39.0	36.0	40.0	33.0	41.0
23	37.860492945490336	39.0	36.0	40.0	34.0	41.0
24	37.92067262394152	40.0	36.0	41.0	34.0	41.0
25	36.822105130497924	39.0	35.0	40.0	31.0	41.0
26	37.17835456787753	39.0	35.0	40.0	32.0	41.0
27	37.54972004008858	39.0	36.0	40.0	33.0	41.0
28	37.63201085032197	39.0	36.0	41.0	33.0	41.0
29	37.74232765811884	40.0	36.0	41.0	33.0	41.0
30	37.29474347510367	39.0	36.0	41.0	33.0	41.0
31	37.454268413440474	39.0	36.0	40.0	33.0	41.0
32	37.45889060258895	39.0	36.0	40.0	33.0	41.0
33	37.61528887181857	40.0	36.0	41.0	33.0	41.0
34	37.66153956946787	40.0	36.0	41.0	33.0	41.0
35	37.597461456751745	40.0	36.0	41.0	33.0	41.0
36	37.50682646086263	40.0	36.0	41.0	33.0	41.0
37	37.551828891382755	40.0	36.0	41.0	33.0	41.0
38	37.42675972658149	40.0	36.0	41.0	33.0	41.0
39	37.386834382557865	40.0	36.0	41.0	33.0	41.0
40	37.27612148999886	40.0	35.0	41.0	33.0	41.0
41	37.15369169002167	39.0	35.0	41.0	33.0	41.0
42	37.26196326013767	39.0	35.0	41.0	33.0	41.0
43	37.22983478464391	39.0	35.0	41.0	33.0	41.0
44	37.240850742058804	39.0	35.0	41.0	33.0	41.0
45	37.246365922307376	39.0	35.0	41.0	33.0	41.0
46	37.08142182427039	39.0	35.0	41.0	33.0	41.0
47	37.04990187900211	39.0	35.0	41.0	33.0	41.0
48	37.04385738548049	39.0	35.0	41.0	33.0	41.0
49	37.013145213075596	39.0	35.0	41.0	33.0	41.0
50	36.92543764365574	39.0	35.0	41.0	32.0	41.0
51	35.96143694750677	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	27.0
10	33.0
11	32.0
12	29.0
13	36.0
14	55.0
15	75.0
16	107.0
17	164.0
18	274.0
19	513.0
20	751.0
21	1334.0
22	1850.0
23	2579.0
24	3549.0
25	4683.0
26	5958.0
27	6798.0
28	7791.0
29	9822.0
30	12910.0
31	18276.0
32	27905.0
33	45667.0
34	62649.0
35	65785.0
36	100491.0
37	168974.0
38	215479.0
39	68548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3178160126267	20.302824804508166	24.802467728093813	12.57689145477132
2	31.535908684458473	23.45649969093386	25.848491577197517	19.159100047410146
3	32.70303845022835	22.306773649561006	27.09975934850058	17.890428551710066
4	30.29400291662416	24.640553078358767	25.447965864695043	19.617478140322028
5	26.736441598502083	28.79296169380247	24.176053675486553	20.294543032208892
6	27.240309426217213	33.51261169890357	23.87070833158296	15.376370543296266
7	80.00492105310536	6.326073779788875	9.079463005083088	4.589542162022673
8	80.84510085158225	6.644982026153596	7.940299224033944	4.569617898230222
9	74.58840191801045	8.453289003846823	9.796736501611344	7.161572576531378
10	41.749854468856334	28.14998409659667	17.08121538009134	13.018946054455654
11	30.00414088614964	25.631365112134	25.934549993698653	18.429944008017713
12	30.189460544556535	22.45572552526241	27.794708067526454	19.56010586265461
13	25.653929941007377	25.17934838055344	28.485575913245437	20.68114576519375
14	19.831123860506146	27.67912333239313	27.91869460064454	24.571058206456183
15	19.145057042207032	27.494283776728217	32.89255900762763	20.468100173437115
16	22.01163048892463	26.7392021892685	30.327970185619723	20.921197136187146
17	21.989545762793238	26.731160468340224	28.441886563724637	22.8374072051419
18	22.12721522405795	27.29684152408615	28.93963308147944	21.63631017037646
19	23.781049144516928	28.466731880622454	26.66874711188194	21.08347186297868
20	26.687591144504925	26.64810269397651	28.5665932509557	18.097712910562862
21	24.894167351813287	27.806710636076122	27.31196476045874	19.987157251651855
22	23.135190930859203	26.132592374768198	27.684044385498495	23.048172308874097
23	22.976877051689062	27.446993656642523	28.80472421098115	20.771405080687266
24	23.425293012704717	25.438123758484316	29.508194753677287	21.62838847513368
25	21.249707437391603	28.114576519375145	27.48660213285643	23.14911391037682
26	20.692548205315937	29.174283296625482	28.856095204373737	21.277073293684847
27	21.044223463821258	28.85813564102718	29.44302080645258	20.654620088698984
28	20.703470542696138	28.83172999021791	29.30091039482449	21.163889072261462
29	22.05339942747748	26.78937292580612	28.705102892018893	22.452124754697504
30	21.744693364379977	27.745857613529296	28.328222239559263	22.181226782531464
31	23.84058188452329	28.096092563808657	25.30645558149444	22.756869970173618
32	24.20930079036914	28.930631155067182	26.262460166475627	20.597607888088053
33	23.16579748066086	27.870804352131355	26.229093025907545	22.734305141300236
34	22.238118957456894	27.333329332477152	27.129405692818263	23.29914601724769
35	22.037195959935428	26.750004500963204	27.568579676050675	23.64421986305069
36	22.65508818887242	28.371071409281583	26.823460220487185	22.150380181358813
37	22.71666136553222	28.662493773667563	28.661653593869087	19.95919126693112
38	21.84083393846283	28.337344191657017	26.845905023675066	22.975916846205088
39	22.160702390311528	27.92925686096825	26.91239925344024	22.997641495279993
40	22.595315397495064	26.09802497734515	29.172482911343028	22.134176713816757
41	19.718299716139253	27.00793969909561	29.444221063307545	23.82953952145759
42	21.13316249677431	26.831741992786455	29.0936260359717	22.941469474467535
43	22.55450666442619	26.544160450336374	27.91461372733765	22.986719157899792
44	21.536328774357713	27.193859485929988	26.908318380133352	24.36149335957895
45	21.64759258481315	26.73104044265473	26.88419321734851	24.73717375518361
46	21.75777616409912	28.623125348824647	26.643661743613134	22.975436743463103
47	20.744999429877993	26.521475595777495	30.51485017793808	22.218674796406432
48	21.50488204475758	25.190030666562645	29.671069608896307	23.634017679783472
49	20.9299590112284	25.410397825134577	29.738043941403458	23.92159922223356
50	19.969753527254834	25.96587669761329	29.65006511393438	24.414304661197498
51	19.67052949331157	25.794960121465994	27.681883923159557	26.852626462062883
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	168.0
1	761.5
2	1355.0
3	2584.0
4	3813.0
5	2847.5
6	1882.0
7	1912.0
8	1942.0
9	1938.0
10	1934.0
11	1846.5
12	1759.0
13	1819.0
14	1879.0
15	1869.5
16	1860.0
17	1750.5
18	1641.0
19	2000.0
20	2359.0
21	2288.5
22	2218.0
23	2631.0
24	3044.0
25	4740.5
26	7492.0
27	8547.0
28	9452.5
29	10358.0
30	13608.0
31	16858.0
32	18561.5
33	20265.0
34	22810.5
35	25356.0
36	28042.0
37	30728.0
38	32211.5
39	33695.0
40	36708.0
41	39721.0
42	44269.0
43	48817.0
44	53097.0
45	57377.0
46	65631.0
47	73885.0
48	71504.5
49	69124.0
50	68523.0
51	67922.0
52	63292.0
53	58662.0
54	53066.5
55	47471.0
56	44769.5
57	42068.0
58	40329.0
59	38590.0
60	36591.5
61	34593.0
62	32183.5
63	29774.0
64	24299.0
65	18824.0
66	15315.0
67	11806.0
68	9520.0
69	7234.0
70	5385.0
71	3536.0
72	2932.5
73	2329.0
74	1839.0
75	1228.5
76	1108.0
77	820.0
78	532.0
79	346.5
80	161.0
81	144.0
82	127.0
83	79.5
84	32.0
85	21.5
86	11.0
87	6.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	833155.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.78221219968789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.87736869988257	17.331850469754997
2	10.984650948989788	5.2247859961675625
3	3.7954102689518985	2.7078975720326564
4	1.8145336078019343	1.7261449321684341
5	1.0497939207771894	1.2483210894932728
6	0.6635377238874685	0.9468236971193813
7	0.4578793173980103	0.7622568161745408
8	0.38009463929103404	0.7231593094066558
9	0.3111754535462871	0.6660396600814731
>10	5.966662641499214	38.6507299230572
>50	1.6155738737917176	24.3623974855539
>100	0.07214197801201558	2.612569783321064
>500	0.007620631480142491	1.1833468278151273
>1k	0.0035562946907331627	1.853676437853756
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG	3196	0.38360209084744135	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC	2988	0.3586367482641285	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC	2326	0.27917974446531557	No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	1958	0.23501029220253133	TruSeq Adapter, Index 16 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1903	0.22840887950021307	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1783	0.21400579724060947	No Hit
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	1188	0.1425905143700752	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT	872	0.10466239775311917	TruSeq Adapter, Index 13 (95% over 22bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	848	0.10178178130119846	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10754301420503988	0.0
2	0.0	0.0	0.0	0.6024089155079186	0.0
3	0.0	0.0	0.0	0.794329986617136	0.0
4	0.0	0.0	0.0	1.142524500243052	0.0
5	0.0	0.0	0.0	2.2468808324981544	0.0
6	0.0	0.0	0.0	2.652207572420498	0.0
7	0.0	0.0	0.0	3.155355246022649	0.0
8	0.0	0.0	0.0	3.8289393930301086	0.0
9	0.0	0.0	0.0	4.067310404426547	0.0
10	0.0	0.0	0.0	5.0291962479970715	0.0
11	0.0	0.0	0.0	5.939711098175009	0.0
12	0.0	0.0	0.0	6.8897143988813605	0.0
13	0.0	0.0	0.0	7.189298509881114	0.0
14	0.0	0.0	0.0	7.2987619350541015	0.0
15	0.0	0.0	0.0	7.565338982542264	0.0
16	0.0	0.0	0.0	8.010634275735008	0.0
17	0.0	0.0	0.0	8.576675408537428	0.0
18	0.0	0.0	0.0	9.145357106420773	0.0
19	0.0	0.0	0.0	9.591972682153981	0.0
20	0.0	0.0	0.0	9.974134464775462	0.0
21	0.0	0.0	0.0	10.441874561156087	0.0
22	0.0	0.0	0.0	10.965066524236187	0.0
23	0.0	0.0	0.0	11.451650653239794	0.0
24	0.0	0.0	0.0	11.82853130569942	0.0
25	0.0	0.0	0.0	12.176365742268846	0.0
26	0.0	0.0	0.0	12.50247552976337	0.0
27	0.0	0.0	0.0	12.840467860122066	0.0
28	0.0	0.0	0.0	13.16693772467308	0.0
29	0.0	0.0	0.0	13.490406947086676	0.0
30	0.0	0.0	0.0	13.86848785640127	0.0
31	0.0	0.0	0.0	14.238527044787585	0.0
32	0.0	0.0	0.0	14.623689469546482	0.0
33	0.0	0.0	0.0	14.988447527770942	0.0
34	1.2002568549669629E-4	0.0	0.0	15.341443068816726	0.0
35	1.2002568549669629E-4	0.0	0.0	15.719764029502313	0.0
36	1.2002568549669629E-4	0.0	0.0	16.087402704178693	0.0
37	1.2002568549669629E-4	0.0	0.0	16.46320312546885	0.0
38	1.2002568549669629E-4	0.0	0.0	16.833482365226157	0.0
39	1.2002568549669629E-4	0.0	0.0	17.191038882320818	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAA	20	7.032169E-4	45.0	39
TCGTTAC	20	7.032169E-4	45.0	17
TCGCAAT	20	7.032169E-4	45.0	34
GCGCGTA	20	7.032169E-4	45.0	1
GACGTAA	20	7.032169E-4	45.0	28
GCGCCGT	20	7.032169E-4	45.0	37
ATTCACG	20	7.032169E-4	45.0	1
CTCGGAA	20	7.032169E-4	45.0	17
GTCACGC	20	7.032169E-4	45.0	22
CGTCGTT	20	7.032169E-4	45.0	20
CGTCGAA	20	7.032169E-4	45.0	17
TCGGTAA	20	7.032169E-4	45.0	30
AATCGGT	20	7.032169E-4	45.0	28
AGTCGAC	20	7.032169E-4	45.0	25
CGTAAGG	135	0.0	41.666668	2
TTGAGCG	65	0.0	41.53846	1
TGCGTCG	65	0.0	41.53846	1
TACGAAT	255	0.0	41.47059	12
CGTTCGG	45	1.9272193E-8	40.0	2
CGAATAT	265	0.0	39.905663	14
>>END_MODULE