FastQCFastQC Report
Sat 18 Jun 2016
SRR3551849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458265
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23940.5224051585872803No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC21700.47352514374870436No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG20360.444284420586342No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC18320.3997686927869246No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC13170.2873883015285915RNA PCR Primer, Index 40 (96% over 27bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC6090.13289254034237832No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGAACCT5620.1226364658003557No Hit
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC5530.12067253663273433Illumina PCR Primer Index 8 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5440.11870860746511298No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCC5140.11216217690637514No Hit
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT4860.10605217505155315Illumina PCR Primer Index 8 (95% over 24bp)
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA4820.10517931764372143No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT4630.10103324495652079No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA207.028842E-445.020
ATCTCCG207.028842E-445.038
CGAACTA253.8871214E-545.014
TCCGCAA207.028842E-445.013
CAACGTG207.028842E-445.023
CGTGCTA253.8871214E-545.033
GACGTAC207.028842E-445.032
ATCAGCG207.028842E-445.020
CCGTGCG207.028842E-445.036
TCCTCGA207.028842E-445.027
ATACTCG502.1827873E-1145.044
AGACGTA207.028842E-445.031
CTAACGG207.028842E-445.02
CCGTAGG253.8871214E-545.02
TAACCGT207.028842E-445.030
ACTACGG253.8871214E-545.01
GGTATCG253.8871214E-545.01
GGTGCGT253.8871214E-545.08
GCGATAT253.8871214E-545.09
CGTACCT207.028842E-445.034