##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551849_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458265
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.322546997916053	28.0	25.0	31.0	16.0	33.0
2	27.733261322597187	31.0	25.0	33.0	16.0	34.0
3	27.62903778381504	31.0	25.0	33.0	16.0	34.0
4	31.696810797246133	35.0	28.0	37.0	19.0	37.0
5	33.28685149422277	35.0	32.0	35.0	28.0	37.0
6	32.99704755981801	35.0	32.0	35.0	28.0	37.0
7	34.09935517658997	35.0	33.0	37.0	31.0	37.0
8	34.098427765594145	35.0	35.0	37.0	31.0	37.0
9	36.426545775915685	39.0	37.0	39.0	32.0	39.0
10	35.56085452740227	37.0	34.0	39.0	31.0	39.0
11	36.08712862644976	37.0	35.0	39.0	31.0	39.0
12	36.02660251164719	37.0	35.0	39.0	31.0	39.0
13	36.10588633214407	37.0	35.0	39.0	32.0	39.0
14	36.985964452882065	39.0	36.0	40.0	32.0	41.0
15	37.24268927367353	39.0	36.0	40.0	32.0	41.0
16	36.771808887870556	38.0	35.0	40.0	32.0	41.0
17	36.99644310606308	38.0	35.0	40.0	32.0	41.0
18	36.80233707570947	38.0	35.0	40.0	32.0	41.0
19	36.35846289810481	38.0	34.0	40.0	30.0	41.0
20	36.89334555333704	38.0	35.0	40.0	32.0	41.0
21	36.86099309351576	38.0	35.0	40.0	32.0	41.0
22	37.29072261682651	39.0	35.0	40.0	33.0	41.0
23	37.66678668456024	39.0	35.0	40.0	33.0	41.0
24	37.721641408355424	39.0	35.0	41.0	33.0	41.0
25	36.685860801064884	38.0	35.0	40.0	31.0	41.0
26	36.99863179601322	39.0	35.0	40.0	32.0	41.0
27	37.37402594568645	39.0	35.0	40.0	33.0	41.0
28	37.4478347680927	39.0	35.0	41.0	33.0	41.0
29	37.558065747984244	39.0	35.0	41.0	33.0	41.0
30	37.1154419386163	39.0	35.0	40.0	32.0	41.0
31	37.20448648707625	39.0	35.0	40.0	33.0	41.0
32	37.165434846649866	39.0	35.0	40.0	33.0	41.0
33	37.2520812193818	39.0	35.0	41.0	33.0	41.0
34	37.24075589451518	40.0	35.0	41.0	33.0	41.0
35	37.17348040980655	40.0	35.0	41.0	33.0	41.0
36	37.073053800748475	40.0	35.0	41.0	33.0	41.0
37	37.13208732938365	40.0	35.0	41.0	33.0	41.0
38	36.96284900657916	39.0	35.0	41.0	32.0	41.0
39	36.882749064405964	39.0	35.0	41.0	32.0	41.0
40	36.752926799995635	39.0	35.0	41.0	32.0	41.0
41	36.6263253794202	39.0	35.0	40.0	31.0	41.0
42	36.77378372775578	39.0	35.0	41.0	32.0	41.0
43	36.748143541400715	39.0	35.0	41.0	32.0	41.0
44	36.74465647605643	39.0	35.0	41.0	32.0	41.0
45	36.73830207412742	39.0	35.0	41.0	32.0	41.0
46	36.55988783782309	39.0	35.0	40.0	32.0	41.0
47	36.53890871002586	39.0	35.0	41.0	31.0	41.0
48	36.55311010005128	39.0	35.0	41.0	32.0	41.0
49	36.53625740564957	39.0	35.0	41.0	32.0	41.0
50	36.455155859600886	39.0	35.0	41.0	32.0	41.0
51	35.545463869158674	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	8.0
10	16.0
11	21.0
12	18.0
13	26.0
14	34.0
15	42.0
16	52.0
17	95.0
18	156.0
19	251.0
20	456.0
21	747.0
22	1122.0
23	1638.0
24	2310.0
25	3274.0
26	4219.0
27	4829.0
28	5216.0
29	6081.0
30	7862.0
31	10817.0
32	16393.0
33	27907.0
34	39169.0
35	37009.0
36	54332.0
37	89328.0
38	110479.0
39	34353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.9740434028346	20.403696551122167	23.50234034892475	13.11991969711848
2	33.89043457388192	24.218301637698712	24.063151233456626	17.82811255496274
3	33.28838117683	22.07696420193556	26.403500158205407	18.231154463029036
4	29.376888917984132	25.031150098741996	26.152335439101833	19.43962554417204
5	26.23678439330955	28.90030877330802	23.73932113515106	21.123585698231373
6	26.35025585632767	34.67316945435501	24.387199546114147	14.589375143203167
7	77.72882502482187	6.355274786422703	11.50818849355722	4.407711695198193
8	77.50428245665717	7.54890729163257	9.729523310748148	5.217286940962107
9	70.3610356453144	9.07946275626548	12.16675940776625	8.392742190653879
10	43.08358700751748	24.066424448735994	19.52603842754738	13.323950116199143
11	33.46600765932376	25.420226288282983	23.49993999105321	17.613826061340056
12	31.17432053506159	22.948730538007485	27.12666252059398	18.750286406336944
13	25.197211220581977	26.44059659803825	29.41245785735328	18.94973432402649
14	20.736037009154092	28.580406533337698	29.20689993780891	21.4766565196993
15	19.746653137376846	26.731258114846213	33.90592779287094	19.616160954906004
16	22.33620285206158	25.476962019792044	31.348237373572058	20.838597754574316
17	22.525831123913022	25.252419451627333	28.697587640339105	23.524161784120544
18	22.75953869485996	26.307922272047833	30.098741994260962	20.833797038831246
19	24.67458784764274	27.34465865819995	27.08672929418568	20.894024199971632
20	26.5492673453133	26.193796165973836	28.882851625151385	18.374084863561478
21	24.67873392032994	28.11997425070647	28.50555901061613	18.695732818347462
22	22.67007081055721	25.501620241563288	28.992831658538183	22.83547728934132
23	22.535868984103086	27.242097912779723	29.056768463661854	21.165264639455337
24	22.516011478074912	25.417389501707525	30.505493546310543	21.56110547390702
25	20.555355525732928	27.136700380784045	29.143181347037196	23.16476274644583
26	20.396495477507557	27.802254154255724	29.820736909866564	21.980513458370158
27	21.401372568273818	28.120410679410384	29.1488549201881	21.3293618321277
28	18.996213980993527	28.176928196567484	31.640862819547642	21.18599500289134
29	20.75720380129401	26.397390156350582	30.138675220669263	22.70673082168614
30	20.85409097356333	28.508832225895496	28.152051760444287	22.485025040096886
31	23.434039256761917	29.036038100225852	25.259838739593903	22.270083903418328
32	24.149345902480007	28.823933750122745	26.086434704810536	20.940285642586712
33	23.01441305794682	29.083390614600724	25.36109019890238	22.541106128550076
34	21.345727908524545	28.44227684854833	27.768867358406162	22.443127884520962
35	21.838237700893586	26.262970115544498	27.040031422866683	24.85876076069523
36	21.37518684603886	28.974283438621757	27.017991773318933	22.63253794202045
37	22.233642106641355	30.53386141206507	26.59160092959314	20.640895551700435
38	21.744841958255595	30.234471321178795	25.157059779821715	22.86362694074389
39	22.81649264072098	28.617284758818588	27.07734607705149	21.488876523408944
40	21.47556544793951	27.928600264039368	27.32043686513262	23.275397422888503
41	18.98268469117214	27.481042628173658	27.786760935266713	25.749511745387494
42	20.560374455827958	25.33054018962827	28.018504577046034	26.090580777497735
43	22.06321669776221	24.62308926058067	28.037925654370287	25.275768387286835
44	20.486399790514223	27.56701908284508	27.210675046097784	24.735906080542915
45	21.319323971937635	27.628555530097216	26.598583788855795	24.453536709109358
46	22.031139188024397	29.074880254874362	26.094945064536894	22.799035492564347
47	20.250073647343786	27.101786084470774	29.47835859164457	23.169781676540865
48	21.314741470546515	25.663535290716073	29.08251775719289	23.93920548154452
49	21.02473459679443	25.54984561334599	29.874199426096254	23.551220363763324
50	20.060881804196264	26.512389119832413	29.19620743456297	24.230521641408355
51	19.753854210991456	26.64506344582283	26.594219501816635	27.006862841369077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	180.0
1	639.5
2	1099.0
3	2733.5
4	4368.0
5	3049.0
6	1730.0
7	1684.0
8	1638.0
9	1682.0
10	1726.0
11	1689.0
12	1652.0
13	1628.0
14	1604.0
15	1582.5
16	1561.0
17	1467.5
18	1374.0
19	1382.5
20	1391.0
21	1441.0
22	1491.0
23	1845.5
24	2200.0
25	2494.5
26	2923.0
27	3057.0
28	4588.0
29	6119.0
30	6764.0
31	7409.0
32	8320.5
33	9232.0
34	10303.5
35	11375.0
36	12561.5
37	13748.0
38	15453.5
39	17159.0
40	19400.5
41	21642.0
42	23664.0
43	25686.0
44	29123.0
45	32560.0
46	38632.5
47	44705.0
48	42212.0
49	39719.0
50	38813.5
51	37908.0
52	34230.5
53	30553.0
54	28632.5
55	26712.0
56	24904.0
57	23096.0
58	21823.0
59	20550.0
60	19530.0
61	18510.0
62	16740.5
63	14971.0
64	12629.5
65	10288.0
66	8571.0
67	6854.0
68	5897.0
69	4940.0
70	3937.0
71	2934.0
72	2308.5
73	1683.0
74	1307.5
75	724.5
76	517.0
77	450.5
78	384.0
79	231.5
80	79.0
81	78.5
82	78.0
83	47.0
84	16.0
85	28.0
86	40.0
87	23.0
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	458265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.561200067751905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8580438748818	19.617582799735338
2	10.740722747413646	5.7057297153261555
3	3.805901862052044	3.0326796238858154
4	1.741286949298522	1.8500268414273366
5	0.9907471227818081	1.3157716272378586
6	0.6821379566361149	1.0871041644011592
7	0.45305897662305017	0.842365308440304
8	0.339373395494967	0.721133172433129
9	0.2784162863614438	0.6655563615750135
>10	6.021359168935437	42.684139448584396
>50	1.0156409956048247	16.641066653669366
>100	0.0650735106673002	3.095203391394432
>500	0.004118576624512671	0.6086700950632156
>1k	0.004118576624512671	2.1329707968264873
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2394	0.5224051585872803	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	2170	0.47352514374870436	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	2036	0.444284420586342	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	1832	0.3997686927869246	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1317	0.2873883015285915	RNA PCR Primer, Index 40 (96% over 27bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	609	0.13289254034237832	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGAACCT	562	0.1226364658003557	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	553	0.12067253663273433	Illumina PCR Primer Index 8 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	544	0.11870860746511298	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCC	514	0.11216217690637514	No Hit
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	486	0.10605217505155315	Illumina PCR Primer Index 8 (95% over 24bp)
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	482	0.10517931764372143	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT	463	0.10103324495652079	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1821435195792826E-4	0.0	0.0	0.11128931949854341	0.0
2	2.1821435195792826E-4	0.0	0.0	0.6391498368847719	0.0
3	2.1821435195792826E-4	0.0	0.0	0.8313966809597068	0.0
4	2.1821435195792826E-4	0.0	0.0	1.2030157223440585	0.0
5	2.1821435195792826E-4	0.0	0.0	2.3866103673638617	0.0
6	2.1821435195792826E-4	0.0	0.0	2.822166213871886	0.0
7	2.1821435195792826E-4	0.0	0.0	3.3471899446826616	0.0
8	2.1821435195792826E-4	0.0	0.0	4.074280165406479	0.0
9	2.1821435195792826E-4	0.0	0.0	4.326754170621802	0.0
10	2.1821435195792826E-4	0.0	0.0	5.404078426238094	0.0
11	2.1821435195792826E-4	0.0	0.0	6.420302663306166	0.0
12	2.1821435195792826E-4	0.0	0.0	7.645794463901891	0.0
13	2.1821435195792826E-4	0.0	0.0	8.015013147414706	0.0
14	2.1821435195792826E-4	0.0	0.0	8.148123902109042	0.0
15	2.1821435195792826E-4	0.0	0.0	8.503376867096549	0.0
16	2.1821435195792826E-4	0.0	0.0	9.04891274699137	0.0
17	2.1821435195792826E-4	0.0	0.0	9.693081513971174	0.0
18	2.1821435195792826E-4	0.0	0.0	10.368236718929005	0.0
19	2.1821435195792826E-4	0.0	0.0	10.899152237242644	0.0
20	2.1821435195792826E-4	0.0	0.0	11.323142723096899	0.0
21	2.1821435195792826E-4	0.0	0.0	11.850348597427253	0.0
22	2.1821435195792826E-4	0.0	0.0	12.37733625740565	0.0
23	2.1821435195792826E-4	0.0	0.0	12.89974141599293	0.0
24	2.1821435195792826E-4	0.0	0.0	13.320458686567816	0.0
25	2.1821435195792826E-4	0.0	0.0	13.70866201870097	0.0
26	2.1821435195792826E-4	0.0	0.0	14.058023196185614	0.0
27	2.1821435195792826E-4	0.0	0.0	14.398219370888024	0.0
28	2.1821435195792826E-4	0.0	0.0	14.768529126160628	0.0
29	2.1821435195792826E-4	0.0	0.0	15.158696387461402	0.0
30	2.1821435195792826E-4	0.0	0.0	15.58028651544412	0.0
31	2.1821435195792826E-4	0.0	0.0	16.024352721678504	0.0
32	2.1821435195792826E-4	0.0	0.0	16.450525351052338	0.0
33	2.1821435195792826E-4	0.0	0.0	16.842656541520736	0.0
34	2.1821435195792826E-4	0.0	0.0	17.261846311631917	0.0
35	2.1821435195792826E-4	0.0	0.0	17.69958430165952	0.0
36	2.1821435195792826E-4	0.0	0.0	18.11637371389916	0.0
37	2.1821435195792826E-4	0.0	0.0	18.549092773831735	0.0
38	2.1821435195792826E-4	0.0	0.0	18.933586461981605	0.0
39	2.1821435195792826E-4	0.0	0.0	19.318080150131475	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAA	20	7.028842E-4	45.0	20
ATCTCCG	20	7.028842E-4	45.0	38
CGAACTA	25	3.8871214E-5	45.0	14
TCCGCAA	20	7.028842E-4	45.0	13
CAACGTG	20	7.028842E-4	45.0	23
CGTGCTA	25	3.8871214E-5	45.0	33
GACGTAC	20	7.028842E-4	45.0	32
ATCAGCG	20	7.028842E-4	45.0	20
CCGTGCG	20	7.028842E-4	45.0	36
TCCTCGA	20	7.028842E-4	45.0	27
ATACTCG	50	2.1827873E-11	45.0	44
AGACGTA	20	7.028842E-4	45.0	31
CTAACGG	20	7.028842E-4	45.0	2
CCGTAGG	25	3.8871214E-5	45.0	2
TAACCGT	20	7.028842E-4	45.0	30
ACTACGG	25	3.8871214E-5	45.0	1
GGTATCG	25	3.8871214E-5	45.0	1
GGTGCGT	25	3.8871214E-5	45.0	8
GCGATAT	25	3.8871214E-5	45.0	9
CGTACCT	20	7.028842E-4	45.0	34
>>END_MODULE