##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551848_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	546823
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.286001137479587	28.0	25.0	31.0	16.0	33.0
2	27.78136069623992	31.0	25.0	33.0	16.0	34.0
3	27.444555185133034	31.0	25.0	33.0	16.0	34.0
4	31.707230676105432	35.0	28.0	37.0	19.0	37.0
5	33.181354844254905	35.0	32.0	35.0	28.0	37.0
6	32.80614385276406	35.0	32.0	35.0	28.0	37.0
7	34.15388343211606	35.0	33.0	37.0	31.0	37.0
8	34.164724234350054	35.0	35.0	37.0	31.0	37.0
9	36.60465635132392	39.0	37.0	39.0	32.0	39.0
10	35.51899060573531	37.0	34.0	39.0	31.0	39.0
11	36.026108996878335	37.0	35.0	39.0	31.0	39.0
12	35.97646770527209	37.0	35.0	39.0	31.0	39.0
13	36.11466050257579	37.0	35.0	39.0	32.0	39.0
14	36.96546780219559	39.0	36.0	40.0	32.0	41.0
15	37.255751861205546	39.0	36.0	40.0	32.0	41.0
16	36.75193252661282	38.0	35.0	40.0	32.0	41.0
17	37.01453121028194	38.0	35.0	40.0	32.0	41.0
18	36.78397580204198	38.0	35.0	40.0	32.0	41.0
19	36.375114067989095	38.0	34.0	40.0	30.0	41.0
20	36.9820362347597	38.0	35.0	40.0	32.0	41.0
21	36.94234880390913	39.0	35.0	40.0	32.0	41.0
22	37.35053024470441	39.0	35.0	40.0	33.0	41.0
23	37.74363185162292	39.0	36.0	40.0	34.0	41.0
24	37.80551476437531	39.0	36.0	41.0	34.0	41.0
25	36.659944808466356	38.0	35.0	40.0	31.0	41.0
26	37.03318806999705	39.0	35.0	40.0	32.0	41.0
27	37.389811694094796	39.0	35.0	40.0	33.0	41.0
28	37.466461725274904	39.0	35.0	41.0	33.0	41.0
29	37.59082006426211	39.0	36.0	41.0	33.0	41.0
30	37.16478275420017	39.0	35.0	40.0	32.0	41.0
31	37.306678760769024	39.0	35.0	40.0	33.0	41.0
32	37.2980123367159	39.0	35.0	40.0	33.0	41.0
33	37.42983927157417	40.0	35.0	41.0	33.0	41.0
34	37.453722685402774	40.0	35.0	41.0	33.0	41.0
35	37.40210269136448	40.0	35.0	41.0	33.0	41.0
36	37.289583649553876	40.0	35.0	41.0	33.0	41.0
37	37.34080863460388	40.0	35.0	41.0	33.0	41.0
38	37.19622254367501	39.0	35.0	41.0	33.0	41.0
39	37.12899055087295	39.0	35.0	41.0	33.0	41.0
40	37.00453163089336	39.0	35.0	41.0	32.0	41.0
41	36.88437574864261	39.0	35.0	41.0	32.0	41.0
42	36.97341370059416	39.0	35.0	41.0	33.0	41.0
43	36.93858524604854	39.0	35.0	41.0	33.0	41.0
44	36.934872893056806	39.0	35.0	41.0	33.0	41.0
45	36.912476249170204	39.0	35.0	41.0	33.0	41.0
46	36.75498653129075	39.0	35.0	40.0	32.0	41.0
47	36.7544251064787	39.0	35.0	41.0	32.0	41.0
48	36.742090219321426	39.0	35.0	41.0	32.0	41.0
49	36.692172787172446	39.0	35.0	40.0	32.0	41.0
50	36.61192012771957	39.0	35.0	40.0	32.0	41.0
51	35.70201326571852	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	22.0
10	30.0
11	33.0
12	21.0
13	33.0
14	49.0
15	69.0
16	85.0
17	122.0
18	213.0
19	368.0
20	568.0
21	832.0
22	1253.0
23	1801.0
24	2529.0
25	3425.0
26	4220.0
27	4838.0
28	5591.0
29	6866.0
30	9015.0
31	12595.0
32	19476.0
33	32903.0
34	45698.0
35	44820.0
36	66527.0
37	107789.0
38	132838.0
39	42186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32525515569023	21.39266270804264	24.65093823778444	12.63114389848269
2	32.84408300309241	22.7905556276894	25.014309932098687	19.35105143711951
3	34.19973190593665	22.129281321378215	26.463407720596976	17.207579052088153
4	30.723835683575857	25.34000947290074	24.40881235792935	19.52734248559406
5	27.035439255481208	28.88411789555304	23.79417105717938	20.286271791786373
6	26.89389436801305	35.15122809391704	22.514597959485975	15.44027957858393
7	79.78267190663158	6.662119186647233	8.870329155869449	4.684879750851738
8	80.9521545362942	6.8075044392792545	7.748028155362888	4.492312869063664
9	74.09253085550534	8.976579258736374	10.153011120600269	6.777878765158013
10	44.31854548912536	24.280068687674074	17.49798380828897	13.90340201491159
11	33.76046728100317	26.672981933825024	22.798784981611966	16.767765803559836
12	30.933592771335515	24.02989632842803	26.521561821649787	18.514949078586675
13	26.10076752440918	26.77594029512292	26.762407579783588	20.36088460068432
14	20.84751372930546	29.051265217446964	27.147907092422958	22.953313960824616
15	20.720415929834697	27.39003297227805	31.780301852701882	20.10924924518537
16	23.336070355489802	25.547206317217817	30.632581292301165	20.484142034991212
17	23.37685137603941	26.03987030538218	27.765291511147115	22.81798680743129
18	23.443051956483178	26.86774330999245	27.94304555587457	21.746159177649808
19	26.081565698589852	27.392044592125785	25.798110174590317	20.728279534694042
20	27.93463332742039	26.293517280728864	27.037633749860557	18.734215641990186
21	25.52068951013399	27.819970996099286	26.6131819619877	20.04615753177902
22	24.509210475784666	25.739041700879444	27.513290406584943	22.238457416750943
23	24.715858696506913	26.905598338036256	28.1683469788213	20.21019598663553
24	23.287425730080848	25.347141579633632	29.707967660467833	21.657465029817693
25	22.33574666756885	27.762914142236156	27.701285425082705	22.200053765112294
26	21.666060132803487	29.348619205849058	27.38985009774644	21.59547056360102
27	22.560133717857514	29.032063391627638	27.736397335152326	20.671405555362522
28	21.009357689782618	28.807310592275748	29.68638846573754	20.496943252204094
29	22.511123343385336	26.805748843775774	28.740561388237147	21.942566424601743
30	21.20320469329198	27.813021763898004	28.464237970970498	22.519535571839516
31	24.33675979247398	28.15993475036712	25.518677890286252	21.984627566872643
32	24.482876543232454	28.89691911276592	25.800121794438052	20.82008254956357
33	23.822699484110945	28.52860980609813	24.81077057841386	22.83792013137706
34	21.67758122829508	28.602491116869626	26.303209630904334	23.41671802393096
35	22.666932444319276	27.209170060513184	27.119195790959782	23.00470170420776
36	23.037436245366415	29.659140160527265	25.982447702455826	21.320975891650498
37	23.33186424126271	30.089626807943336	27.07329428352502	19.505214667268934
38	23.002872958891636	29.42542650912635	25.493075455860488	22.078625076121526
39	23.373559634470386	27.716098262143323	25.9255737231243	22.984768380261986
40	22.255830497254138	27.546939320401663	28.14036717548457	22.056863006859622
41	21.05653931893867	27.053360959579244	27.685741089895632	24.204358631586455
42	21.797546921032946	27.278662382525976	27.899887166413993	23.023903530027084
43	23.472494756072805	25.65619953805893	27.486042101374668	23.38526360449359
44	22.256927744443814	27.940668186963606	26.3694102113481	23.432993857244483
45	22.56452270661622	27.450015818646982	25.882781082726954	24.102680392009844
46	22.322762575824353	28.268196473081787	26.17464883518067	23.234392115913195
47	22.01023000129841	27.151747457586822	28.70014611675076	22.13787642436401
48	21.766823999722032	26.08046845140018	28.689356519385615	23.463351029492173
49	21.35700217437818	26.559233975161984	29.712356649226535	22.371407201233307
50	21.065865920050914	26.73150178394106	29.00792395345477	23.19470834255326
51	20.705237343710852	27.227091764611217	26.66237521099149	25.40529568068644
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	204.0
1	705.0
2	1206.0
3	2121.0
4	3036.0
5	2186.5
6	1337.0
7	1401.0
8	1465.0
9	1485.5
10	1506.0
11	1477.0
12	1448.0
13	1414.0
14	1380.0
15	1423.0
16	1466.0
17	1409.0
18	1352.0
19	1439.5
20	1527.0
21	1624.0
22	1721.0
23	1788.5
24	1856.0
25	2594.0
26	4270.0
27	5208.0
28	6890.0
29	8572.0
30	9539.0
31	10506.0
32	10791.5
33	11077.0
34	13459.5
35	15842.0
36	15906.0
37	15970.0
38	17216.0
39	18462.0
40	22464.0
41	26466.0
42	29384.5
43	32303.0
44	34807.0
45	37311.0
46	40798.5
47	44286.0
48	44859.5
49	45433.0
50	44338.5
51	43244.0
52	40070.5
53	36897.0
54	34417.5
55	31938.0
56	30384.5
57	28831.0
58	28238.0
59	27645.0
60	25899.0
61	24153.0
62	22183.0
63	20213.0
64	17454.0
65	14695.0
66	12080.0
67	9465.0
68	8111.5
69	6758.0
70	5348.5
71	3939.0
72	2949.5
73	1960.0
74	1674.0
75	1051.0
76	714.0
77	504.5
78	295.0
79	268.5
80	242.0
81	190.5
82	139.0
83	82.0
84	25.0
85	14.5
86	4.0
87	3.0
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	546823.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.427380343086536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.93012211315309	17.319890895556703
2	9.958383705195072	4.665976853280006
3	3.421641401753909	2.4048028354962177
4	1.593379441071553	1.4931482478735163
5	0.8793768462256567	1.0300747920716191
6	0.6001302527060182	0.8435687811321915
7	0.4957525837695004	0.8129929035225167
8	0.3641417424906412	0.6824709680098022
9	0.30305130443122685	0.6389728355140972
>10	6.904325869317445	43.75677250905445
>50	1.4706047102100583	21.417551633610472
>100	0.06969319446682386	2.815998848586437
>500	0.005481487205255808	0.9271699140557558
>1k	0.003915348003754149	1.1906079822362199
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1572	0.28747876369501646	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1385	0.25328122628345917	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC	1309	0.23938276188090113	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG	1152	0.210671460417722	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC	1072	0.1960414978887135	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	912	0.16678157283069658	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	788	0.14410513091073346	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	759	0.13880176949396789	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	759	0.13880176949396789	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	674	0.12325743430689638	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	627	0.1146623313211039	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07936754671987097	0.0
2	0.0	0.0	0.0	0.40177534595289516	0.0
3	0.0	0.0	0.0	0.521558164159152	0.0
4	0.0	0.0	0.0	0.755454690091675	0.0
5	1.828745316126059E-4	0.0	0.0	1.4593387622685952	0.0
6	1.828745316126059E-4	0.0	0.0	1.7704083405416378	0.0
7	1.828745316126059E-4	0.0	0.0	2.1052516079243193	0.0
8	1.828745316126059E-4	0.0	0.0	2.578713770269356	0.0
9	1.828745316126059E-4	0.0	0.0	2.7352543693297466	0.0
10	1.828745316126059E-4	0.0	0.0	3.3687317468358136	0.0
11	1.828745316126059E-4	0.0	0.0	4.050122251624383	0.0
12	1.828745316126059E-4	0.0	0.0	4.807771436095409	0.0
13	1.828745316126059E-4	0.0	0.0	5.0548349283040395	0.0
14	1.828745316126059E-4	0.0	0.0	5.137859965656163	0.0
15	1.828745316126059E-4	0.0	0.0	5.339570574024867	0.0
16	1.828745316126059E-4	0.0	0.0	5.710257249603619	0.0
17	1.828745316126059E-4	0.0	0.0	6.14257264233582	0.0
18	1.828745316126059E-4	0.0	0.0	6.6213381660976225	0.0
19	1.828745316126059E-4	0.0	0.0	6.956730057075141	0.0
20	1.828745316126059E-4	0.0	0.0	7.248780684060473	0.0
21	1.828745316126059E-4	0.0	0.0	7.620198857765676	0.0
22	1.828745316126059E-4	0.0	0.0	7.993080027723779	0.0
23	1.828745316126059E-4	0.0	0.0	8.347856619052234	0.0
24	1.828745316126059E-4	0.0	0.0	8.660389193578178	0.0
25	1.828745316126059E-4	0.0	0.0	8.940552976008691	0.0
26	1.828745316126059E-4	0.0	0.0	9.209744286542445	0.0
27	1.828745316126059E-4	0.0	0.0	9.459916645788491	0.0
28	1.828745316126059E-4	0.0	0.0	9.725267591158381	0.0
29	1.828745316126059E-4	0.0	0.0	10.006162871715345	0.0
30	1.828745316126059E-4	0.0	0.0	10.311929088571622	0.0
31	1.828745316126059E-4	0.0	0.0	10.620804172465313	0.0
32	3.657490632252118E-4	0.0	0.0	10.936994237623509	0.0
33	3.657490632252118E-4	0.0	0.0	11.229776362735292	0.0
34	3.657490632252118E-4	0.0	0.0	11.52786184926384	0.0
35	3.657490632252118E-4	0.0	0.0	11.843869039890421	0.0
36	3.657490632252118E-4	0.0	0.0	12.13921140844478	0.0
37	3.657490632252118E-4	0.0	0.0	12.432725031683013	0.0
38	3.657490632252118E-4	0.0	0.0	12.741600115576704	0.0
39	3.657490632252118E-4	0.0	0.0	13.043891716332341	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	40	6.8030204E-9	45.000004	1
CTATGCG	20	7.030038E-4	45.000004	1
TCGCACC	20	7.030038E-4	45.000004	41
CGATTGA	40	6.8030204E-9	45.000004	10
GTTAAGC	20	7.030038E-4	45.000004	37
TTGCGTA	20	7.030038E-4	45.000004	41
TTGCGAA	20	7.030038E-4	45.000004	1
TCCCGAT	20	7.030038E-4	45.000004	44
GTATACG	20	7.030038E-4	45.000004	1
ACGTATA	20	7.030038E-4	45.000004	40
ACCGTAC	20	7.030038E-4	45.000004	45
CATCCGT	20	7.030038E-4	45.000004	18
CGATATT	20	7.030038E-4	45.000004	10
TGCGTAA	20	7.030038E-4	45.000004	42
CGTATAC	25	3.888114E-5	45.0	17
ATTACGG	25	3.888114E-5	45.0	2
CATCGTA	25	3.888114E-5	45.0	14
CGATAAT	35	1.2103737E-7	45.0	10
CGAAAGG	75	0.0	42.0	2
TAGGGCG	210	0.0	40.714287	5
>>END_MODULE