##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551847_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	360653
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.265742971776195	28.0	25.0	31.0	16.0	33.0
2	27.63772379544881	31.0	25.0	33.0	16.0	34.0
3	27.565457655974026	31.0	25.0	33.0	16.0	34.0
4	31.739896798307516	35.0	28.0	37.0	19.0	37.0
5	33.183018025636834	35.0	32.0	35.0	28.0	37.0
6	32.86409928657186	35.0	32.0	35.0	28.0	37.0
7	34.09366066551505	35.0	33.0	37.0	31.0	37.0
8	34.094769764843214	35.0	35.0	37.0	31.0	37.0
9	36.50178980904082	39.0	37.0	39.0	32.0	39.0
10	35.429764898669916	37.0	34.0	39.0	31.0	39.0
11	35.93222294005595	37.0	35.0	39.0	31.0	39.0
12	35.8881806057346	37.0	35.0	39.0	31.0	39.0
13	35.87058474489329	37.0	35.0	39.0	31.0	39.0
14	36.681186625371204	39.0	35.0	40.0	31.0	41.0
15	37.013578148525035	39.0	35.0	40.0	32.0	41.0
16	36.61970370411448	38.0	35.0	40.0	31.0	41.0
17	36.78081147252345	38.0	35.0	40.0	32.0	41.0
18	36.61817037429329	38.0	35.0	40.0	31.0	41.0
19	36.26119289178241	38.0	34.0	40.0	30.0	41.0
20	36.586874364000856	38.0	35.0	40.0	31.0	41.0
21	36.73087427527291	38.0	35.0	40.0	31.0	41.0
22	37.085852606244785	39.0	35.0	40.0	32.0	41.0
23	37.41104330201052	39.0	35.0	40.0	33.0	41.0
24	37.47517697066155	39.0	35.0	41.0	33.0	41.0
25	36.45984367244969	38.0	35.0	40.0	31.0	41.0
26	36.76421657382581	38.0	35.0	40.0	31.0	41.0
27	37.01349496607543	39.0	35.0	40.0	32.0	41.0
28	37.09622823045975	39.0	35.0	40.0	32.0	41.0
29	37.26746762123149	39.0	35.0	41.0	33.0	41.0
30	36.83291418621223	39.0	35.0	40.0	31.0	41.0
31	37.02568674044026	39.0	35.0	40.0	32.0	41.0
32	36.896970217910294	39.0	35.0	40.0	32.0	41.0
33	36.93627115260375	39.0	35.0	41.0	32.0	41.0
34	36.932164712341226	39.0	35.0	41.0	32.0	41.0
35	36.884004846764064	39.0	35.0	41.0	32.0	41.0
36	36.75438718102996	39.0	35.0	41.0	31.0	41.0
37	36.7543927265266	39.0	35.0	41.0	32.0	41.0
38	36.69594041918409	39.0	35.0	41.0	31.0	41.0
39	36.58617840417243	39.0	35.0	41.0	31.0	41.0
40	36.42319903064719	39.0	35.0	41.0	31.0	41.0
41	36.30961339570169	39.0	35.0	40.0	30.0	41.0
42	36.398995710558346	39.0	35.0	40.0	31.0	41.0
43	36.38853135839713	39.0	35.0	40.0	31.0	41.0
44	36.47755321597214	39.0	35.0	40.0	31.0	41.0
45	36.51222643371884	39.0	35.0	40.0	32.0	41.0
46	36.355740836759985	39.0	35.0	40.0	31.0	41.0
47	36.283377651093986	39.0	35.0	40.0	31.0	41.0
48	36.32564542649028	38.0	35.0	40.0	31.0	41.0
49	36.30121751378749	38.0	35.0	40.0	31.0	41.0
50	36.1625385065423	38.0	35.0	40.0	31.0	41.0
51	35.25047344677571	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	13.0
10	13.0
11	16.0
12	22.0
13	25.0
14	25.0
15	41.0
16	47.0
17	97.0
18	204.0
19	427.0
20	746.0
21	926.0
22	1279.0
23	1663.0
24	2208.0
25	3007.0
26	3465.0
27	3749.0
28	4222.0
29	5071.0
30	6743.0
31	9626.0
32	14206.0
33	23414.0
34	32430.0
35	29156.0
36	42075.0
37	67424.0
38	82077.0
39	26235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.10623785189642	18.79951088719628	23.381477486670015	12.712773774237288
2	32.082084441277345	27.04538711725675	23.694243497212	17.178284944253893
3	32.34743645554037	24.940316592403224	24.690214693902448	18.02203225815396
4	28.10152695250005	23.86282659509279	28.462261508985094	19.57338494342207
5	29.17374872800171	26.7525848946217	23.77603957266403	20.29762680471256
6	25.42083387632988	36.5689457733611	23.06372052915129	14.946499821157733
7	75.92478088356398	6.306616054767325	13.17526819408129	4.593334867587403
8	76.53229004056531	9.578459072848416	8.935181462513828	4.954069424072446
9	70.96987963499541	8.178498445874565	10.243364120082184	10.608257799047838
10	45.01418260765889	22.262396264553463	18.340343765336765	14.383077362450889
11	35.56798362969392	25.503184501445986	21.906098105381073	17.02273376347902
12	31.785677645825768	22.305651138351823	26.142302989299964	19.766368226522445
13	26.095998092349156	26.685484385267834	27.69199202557583	19.52652549680718
14	20.034492989105875	31.25608271662789	26.57651537627581	22.132908917990424
15	17.912231424665816	26.53436960180561	35.94729559992569	19.60610337360288
16	21.927170992616173	23.619933842225073	31.664508544223953	22.788386620934805
17	21.97375316439902	24.991335161498725	28.41041111539347	24.624500558708785
18	22.03558545194411	26.210512597981996	29.227262770585575	22.526639179488317
19	24.829683934418956	27.74162422051113	26.073261556121814	21.355430288948103
20	28.243491666504923	25.908283031057554	28.166963812861674	17.681261489575853
21	25.228959692557666	28.10624062464474	26.518010386715208	20.146789296082385
22	22.6999359495138	23.90608146889115	28.663285762214652	24.7306968193804
23	23.077306995921287	28.148663673946977	27.375898717049353	21.39813061308238
24	23.70089809318098	26.11568460542405	28.374642662060207	21.808774639334764
25	20.8793494023341	29.40499593792371	27.832847640252538	21.882807019489647
26	20.654756788381075	27.253897790951413	29.480691967070843	22.610653453596672
27	22.839682464862346	29.837267401075273	27.105278480977564	20.21777165308482
28	19.20544124130397	30.092914796216863	30.232938586397452	20.46870537608172
29	22.40852010103895	25.873346402220417	30.353275863503143	21.364857633237488
30	20.427391426107643	31.467920688307043	27.341239363044256	20.763448522541058
31	23.443309774215106	27.788760941958056	23.710325437470367	25.057603846356468
32	27.90854366939967	28.413738413377953	24.160619764704578	19.517098152517796
33	25.342919648526426	29.61655663477082	23.416136840675108	21.62438687602765
34	22.620635347550138	28.97355629926827	26.499432972968478	21.906375380213113
35	24.363862216590462	27.313789154672218	26.14618483694853	22.176163791788785
36	23.601356428478343	28.941669693583584	25.331274105580707	22.12569977235736
37	23.911626965531966	29.691698114253867	26.95139094919493	19.445283971019233
38	22.53883927209811	28.570121418648952	24.241029465996398	24.650009843256537
39	24.466453904445547	29.038993159629893	25.66566755302188	20.82888538290268
40	23.690361649563428	27.416658117359344	27.987844271363333	20.905135961713892
41	21.827906602745575	27.6734146118291	26.80609893720557	23.692579848219758
42	24.011168630234604	25.794045800256754	27.3864351606669	22.80835040884174
43	24.71905127643469	26.89898600593923	25.59246699736312	22.789495720262966
44	22.417115620832213	28.095426906195154	26.428727890798083	23.058729582174557
45	21.121410330705693	27.54004541761749	25.57527595777659	25.763268293900232
46	24.040282487598883	28.510507329760188	25.450779558190284	21.99843062445065
47	20.65364768905291	28.959692557666234	30.05354177006707	20.333117983213782
48	23.565587975145082	25.90107388542449	27.00130041896227	23.532037720468153
49	21.86894327788761	24.631155154677764	30.331093876939885	23.16880769049474
50	21.44415823520115	26.65165685575886	28.155595544747996	23.74858936429199
51	20.327017936908884	27.982853324386596	26.49166927767134	25.198459461033178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	104.0
1	287.5
2	471.0
3	1547.0
4	2623.0
5	1882.5
6	1142.0
7	1179.5
8	1217.0
9	1270.5
10	1324.0
11	1288.5
12	1253.0
13	1203.5
14	1154.0
15	1078.0
16	1002.0
17	958.0
18	914.0
19	957.5
20	1001.0
21	1125.0
22	1249.0
23	1149.0
24	1049.0
25	1244.5
26	2079.0
27	2718.0
28	3604.0
29	4490.0
30	5108.5
31	5727.0
32	6382.0
33	7037.0
34	7581.5
35	8126.0
36	9329.0
37	10532.0
38	11444.5
39	12357.0
40	14133.5
41	15910.0
42	18233.0
43	20556.0
44	23303.0
45	26050.0
46	33432.5
47	40815.0
48	35030.5
49	29246.0
50	28259.5
51	27273.0
52	25296.0
53	23319.0
54	22648.5
55	21978.0
56	20596.0
57	19214.0
58	18107.0
59	17000.0
60	16788.5
61	16577.0
62	14939.0
63	13301.0
64	10963.0
65	8625.0
66	6927.5
67	5230.0
68	4224.5
69	3219.0
70	2828.0
71	2437.0
72	1909.5
73	1382.0
74	1065.5
75	548.0
76	347.0
77	329.0
78	311.0
79	218.0
80	125.0
81	76.5
82	28.0
83	21.0
84	14.0
85	10.5
86	7.0
87	4.5
88	2.0
89	3.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	360653.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.135665029820906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6760793143855	18.264925011021674
2	9.651218895756267	4.658771727949026
3	3.227029386761023	2.336595009607573
4	1.3739861682328884	1.3264827964830461
5	0.8501252211474393	1.0259168785508508
6	0.5916412012039611	0.8567792310059809
7	0.39289571031408677	0.6637959479056046
8	0.29984146313443466	0.5789498493011288
9	0.26422810927555546	0.573958902324395
>10	6.091032327734761	39.66804657108079
>50	1.5106954943363278	23.06011595633476
>100	0.05973852905360384	2.1946302956026984
>500	0.0045952714656618335	0.7608421391198742
>1k	0.00689290719849275	4.0301896837125994
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG	3787	1.0500397889383977	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC	3687	1.0223123057343206	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC	3398	0.9421798792745382	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1406	0.38984841384932334	No Hit
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	1248	0.3460389903868816	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGAAGGT	1009	0.2797703055291374	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCT	769	0.2132243458393525	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTA	767	0.21266979617527096	No Hit
GAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	706	0.19575603142078396	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTC	502	0.1391919656844668	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07846877746753805	0.0
2	0.0	0.0	0.0	0.6061227828411243	0.0
3	0.0	0.0	0.0	0.7444829240294688	0.0
4	0.0	0.0	0.0	1.1412632086798113	0.0
5	0.0	0.0	0.0	2.626347209090178	0.0
6	0.0	0.0	0.0	2.932181348831148	0.0
7	0.0	0.0	0.0	3.4271169240239234	0.0
8	0.0	0.0	0.0	3.9702983199917927	0.0
9	0.0	0.0	0.0	4.136663219216255	0.0
10	0.0	0.0	0.0	5.624242693114988	0.0
11	0.0	0.0	0.0	6.261974806808761	0.0
12	0.0	0.0	0.0	7.780886336728101	0.0
13	0.0	0.0	0.0	8.067033963394177	0.0
14	0.0	0.0	0.0	8.195134935797013	0.0
15	0.0	0.0	0.0	8.589419746958988	0.0
16	0.0	0.0	0.0	8.975940862823823	0.0
17	0.0	0.0	0.0	9.418471494760892	0.0
18	0.0	0.0	0.0	9.839374689798781	0.0
19	0.0	0.0	0.0	10.398915300857057	0.0
20	0.0	0.0	0.0	10.703640341269864	0.0
21	0.0	0.0	0.0	11.030824643077972	0.0
22	0.0	0.0	0.0	11.434259523697294	0.0
23	0.0	0.0	0.0	11.809966921112537	0.0
24	0.0	0.0	0.0	12.11275103770106	0.0
25	0.0	0.0	0.0	12.367843883178567	0.0
26	0.0	0.0	0.0	12.619054881007505	0.0
27	0.0	0.0	0.0	12.871097703332566	0.0
28	0.0	0.0	0.0	13.118426853512933	0.0
29	0.0	0.0	0.0	13.355496834907791	0.0
30	0.0	0.0	0.0	13.637208064261214	0.0
31	0.0	0.0	0.0	13.933337584880759	0.0
32	0.0	0.0	0.0	14.220871585707037	0.0
33	0.0	0.0	0.0	14.525319351287804	0.0
34	0.0	0.0	0.0	14.807307855473267	0.0
35	0.0	0.0	0.0	15.101219177436484	0.0
36	0.0	0.0	0.0	15.397071423223986	0.0
37	0.0	0.0	0.0	15.7045692119572	0.0
38	0.0	0.0	0.0	16.000976007408784	0.0
39	2.7727483204077047E-4	0.0	0.0	16.30570104782159	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAC	25	3.8854632E-5	45.000004	10
ATATGCA	25	3.8854632E-5	45.000004	28
CGTTCAT	25	3.8854632E-5	45.000004	26
GTACTAT	25	3.8854632E-5	45.000004	13
GACTCGA	25	3.8854632E-5	45.000004	9
AGTGTCG	25	3.8854632E-5	45.000004	1
TATCACG	20	7.026843E-4	45.0	1
AACGTCA	20	7.026843E-4	45.0	45
AGCCGTA	20	7.026843E-4	45.0	9
CGAACGA	20	7.026843E-4	45.0	34
GCGACGC	20	7.026843E-4	45.0	19
CGTATCG	20	7.026843E-4	45.0	2
GCGAACC	35	1.2088276E-7	45.0	33
GCGAACA	35	1.2088276E-7	45.0	33
CGGCACT	20	7.026843E-4	45.0	26
TAGCGAC	20	7.026843E-4	45.0	41
CCGGAAA	20	7.026843E-4	45.0	35
CACGCAT	20	7.026843E-4	45.0	20
ACCGAAC	20	7.026843E-4	45.0	40
CGCGCGA	20	7.026843E-4	45.0	40
>>END_MODULE