FastQCFastQC Report
Sat 18 Jun 2016
SRR3551846_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551846_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626623
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCG40310.6432895058113092No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC37260.5946159014271739No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTC35930.573391018203928No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.23586749927787523No Hit
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC14350.2290053189876529No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG11380.18160839930867523No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTACCGT11190.17857627313392582No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCT8010.12782805610390938No Hit
GAACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT7830.12495551551730466No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTA6850.1093161278791235No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAT207.0308277E-445.043
GGCGTAT207.0308277E-445.025
GTAATCG207.0308277E-445.034
TGGCACG207.0308277E-445.02
ATATACG207.0308277E-445.01
TCGTCGT207.0308277E-445.029
GTCGTAA207.0308277E-445.031
AACGAGC406.8066583E-945.015
ATACGCA207.0308277E-445.038
CCCGCCA207.0308277E-445.040
TCGCCGT253.8887687E-544.99999613
TACGAAT900.044.99999612
CGTATGG603.6379788E-1241.2499962
GGCCGAT1150.041.0869568
TACGGGA1550.040.645164
CTAGCGG501.0804797E-940.4999962
CGAATAT950.040.26315314
CGTAAGG900.039.9999962
CCGATAA800.039.37511
ACGGGAC1600.039.3755